Standard name | |
---|---|
Human Ortholog | |
Description | Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p; essential for cell cycle progression from G2/M to G1 under nitrogen starvation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 3 | 14 | 12 | 5 | 18 | – | 6 | 5 | 3 | 7 | 4 | 3 | 5 | 1 | 0 | 0 | 2 | 2 | 0 | 3 | 3 | 5 |
Bud | 1 | 2 | 2 | 6 | 10 | 8 | 25 | – | 32 | 19 | 19 | 12 | 18 | 9 | 5 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 8 | 12 |
Bud Neck | 1 | 0 | 0 | 0 | 2 | 0 | 0 | – | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 3 | 2 | 2 | 3 | 4 | 5 | – | 5 | 3 | 3 | 1 | 1 | 1 | 1 | 6 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 306 | 424 | 286 | 122 | 169 | 227 | 330 | – | 219 | 228 | 183 | 164 | 369 | 411 | 444 | 325 | 246 | 219 | 284 | 408 | 267 | 311 | 427 | 481 |
Endoplasmic Reticulum | 6 | 6 | 3 | 6 | 3 | 2 | 4 | – | 1 | 2 | 2 | 6 | 2 | 0 | 12 | 6 | 10 | 6 | 0 | 4 | 0 | 30 | 39 | 25 |
Endosome | 8 | 9 | 1 | 44 | 60 | 101 | 74 | – | 52 | 30 | 24 | 24 | 23 | 48 | 76 | 5 | 1 | 4 | 19 | 18 | 8 | 12 | 31 | 29 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 3 | 0 | 2 | 2 | 4 |
Mitochondria | 2 | 3 | 0 | 1 | 2 | 3 | 9 | – | 27 | 38 | 32 | 8 | 2 | 0 | 1 | 4 | 0 | 2 | 3 | 2 | 6 | 13 | 13 | 10 |
Nucleus | 3 | 1 | 1 | 0 | 0 | 2 | 5 | – | 2 | 5 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 2 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 3 | 8 | 2 | – | 6 | 2 | 3 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 0 | 1 | 1 | 2 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 35 | 58 | 32 | 29 | – | 20 | 22 | 13 | 14 | 0 | 0 | 6 | 4 | 1 | 0 | 3 | 1 | 0 | 2 | 2 | 7 |
Vac/Vac Membrane | 1 | 2 | 3 | 3 | 29 | 56 | 59 | – | 40 | 48 | 30 | 34 | 1 | 8 | 6 | 10 | 11 | 11 | 3 | 10 | 7 | 25 | 34 | 31 |
Unique Cell Count | 320 | 444 | 296 | 200 | 304 | 364 | 463 | 332 | 340 | 258 | 235 | 402 | 464 | 537 | 346 | 258 | 230 | 331 | 457 | 297 | 417 | 575 | 623 | |
Labelled Cell Count | 334 | 453 | 302 | 235 | 352 | 451 | 560 | 413 | 404 | 318 | 273 | 423 | 487 | 561 | 364 | 278 | 250 | 331 | 457 | 297 | 417 | 575 | 623 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 7.9 | 7.1 | 7.0 | 8.1 | 6.5 | 6.3 | – | 5.3 | 4.9 | 4.7 | 5.6 | 6.3 | 6.9 | 7.2 | 13.7 | 15.0 | 12.5 | 5.9 | 6.4 | 7.0 |
Std Deviation (1e-4) | 1.0 | 1.2 | 1.1 | 1.6 | 1.5 | 1.4 | 1.1 | – | 1.1 | 1.0 | 0.9 | 1.4 | 1.2 | 1.4 | 1.2 | 3.1 | 2.9 | 2.8 | 1.4 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.18 | -0.12 | -0.18 | – | -0.43 | -0.54 | -0.59 | -0.34 | -0.18 | -0.04 | 0.02 | 0.95 | 1.08 | 0.82 | -0.27 | -0.14 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.6 | 2.3 | 0 | 2.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2.3 | 0 | 3.4 | – | 5.0 | 0 | 0 | 3.2 | 3.0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -10.2 | -11.7 | -10.5 | -8.7 | – | -9.7 | -9.4 | -8.4 | -8.5 | -2.6 | -3.9 | -5.8 | -1.6 | -0.8 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 2.2 | 0 |
Endosome | 8.2 | 7.9 | 9.7 | 7.0 | – | 6.9 | 0 | 0 | 5.3 | 3.9 | 5.5 | 6.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7.2 | 7.7 | 5.0 | 4.1 | – | 4.0 | 0 | 0 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.6 | 6.4 | 5.8 | – | 5.5 | 6.1 | 5.3 | 6.0 | 0 | 0 | 0 | 1.7 | 2.4 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8588 | 5.1824 | 4.4671 | 4.4732 | 3.2326 | 4.9136 | 1.9433 | 3.5697 | 2.7612 | 2.7549 | 2.5493 | 3.1229 | 1.9386 | 3.3603 | 2.6073 | 2.8428 | 2.2969 | 2.9061 |
Actin | 0.0705 | 0.0002 | 0.0147 | 0.0054 | 0.0001 | 0.001 | 0.0338 | 0.0092 | 0.0027 | 0.0028 | 0.0166 | 0.0006 | 0.0043 | 0.0036 | 0.0048 | 0.0008 | 0.0017 | 0.002 |
Bud | 0.0006 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0007 | 0.0051 | 0.0027 | 0.0019 | 0.0012 | 0.0006 | 0.0102 | 0.0352 | 0.0233 | 0.0004 | 0.0681 | 0.0079 |
Bud Neck | 0.0039 | 0.0001 | 0.0004 | 0.0009 | 0.0001 | 0.0017 | 0.0163 | 0.0003 | 0.0031 | 0.0004 | 0.0011 | 0.0007 | 0.0013 | 0.0002 | 0.0006 | 0.0003 | 0.0009 | 0.0011 |
Bud Periphery | 0.0012 | 0.0001 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0007 | 0.0017 | 0.0035 | 0.005 | 0.0008 | 0.0004 | 0.0066 | 0.0109 | 0.0091 | 0.0002 | 0.0264 | 0.0047 |
Bud Site | 0.002 | 0.0006 | 0.001 | 0.0044 | 0.0001 | 0.0001 | 0.0145 | 0.0079 | 0.0059 | 0.0034 | 0.0011 | 0.0005 | 0.0064 | 0.0171 | 0.0304 | 0.0014 | 0.0047 | 0.0004 |
Cell Periphery | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0007 | 0.0007 | 0.0005 | 0.0006 | 0.0002 |
Cytoplasm | 0.6007 | 0.8445 | 0.7336 | 0.7341 | 0.7559 | 0.8118 | 0.5963 | 0.8546 | 0.8209 | 0.8604 | 0.5755 | 0.8259 | 0.6247 | 0.7827 | 0.7089 | 0.7238 | 0.659 | 0.7961 |
Cytoplasmic Foci | 0.0184 | 0.0289 | 0.0447 | 0.0275 | 0.0349 | 0.021 | 0.0665 | 0.0502 | 0.0346 | 0.0177 | 0.0969 | 0.0551 | 0.0414 | 0.0319 | 0.0695 | 0.0506 | 0.0622 | 0.0351 |
Eisosomes | 0.001 | 0 | 0.0004 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0012 | 0 | 0 |
Endoplasmic Reticulum | 0.012 | 0.0031 | 0.0036 | 0.0043 | 0.0019 | 0.0027 | 0.0054 | 0.0017 | 0.0034 | 0.0013 | 0.0032 | 0.0035 | 0.0081 | 0.002 | 0.0039 | 0.0046 | 0.0031 | 0.0032 |
Endosome | 0.0391 | 0.0269 | 0.0442 | 0.0748 | 0.0385 | 0.0338 | 0.0729 | 0.0292 | 0.0528 | 0.0226 | 0.118 | 0.0553 | 0.0465 | 0.0227 | 0.0449 | 0.0947 | 0.0475 | 0.0272 |
Golgi | 0.005 | 0.0009 | 0.0021 | 0.0017 | 0.0013 | 0.0008 | 0.012 | 0.0021 | 0.0058 | 0.0038 | 0.0306 | 0.0036 | 0.0017 | 0.0011 | 0.0019 | 0.0014 | 0.0007 | 0.0006 |
Lipid Particles | 0.0042 | 0.0005 | 0.0022 | 0.0008 | 0.0001 | 0.0004 | 0.0195 | 0.0001 | 0.004 | 0.0017 | 0.047 | 0.0003 | 0.0019 | 0.0002 | 0.0007 | 0.0022 | 0.0005 | 0.0003 |
Mitochondria | 0.0021 | 0.0001 | 0.001 | 0.0031 | 0.0003 | 0.0004 | 0.0126 | 0.0004 | 0.0094 | 0.0495 | 0.0388 | 0.0024 | 0.0017 | 0.0042 | 0.0043 | 0.005 | 0.0016 | 0.0008 |
None | 0.2067 | 0.0875 | 0.1284 | 0.1072 | 0.1639 | 0.1144 | 0.0752 | 0.03 | 0.0329 | 0.0147 | 0.0281 | 0.0262 | 0.2266 | 0.0758 | 0.0781 | 0.0727 | 0.0988 | 0.1077 |
Nuclear Periphery | 0.0119 | 0.0006 | 0.0015 | 0.004 | 0.0002 | 0.0026 | 0.0201 | 0.0004 | 0.0011 | 0.0006 | 0.0075 | 0.0072 | 0.0027 | 0.0007 | 0.0021 | 0.0023 | 0.0017 | 0.0021 |
Nucleolus | 0.0006 | 0 | 0.0002 | 0.0009 | 0 | 0.0002 | 0.0038 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0.0005 | 0.0004 | 0.0007 | 0.001 | 0.0161 | 0.0006 | 0.0003 |
Nucleus | 0.0038 | 0.0008 | 0.0016 | 0.006 | 0.0004 | 0.0018 | 0.0162 | 0.0012 | 0.0017 | 0.0011 | 0.0019 | 0.0065 | 0.0033 | 0.0023 | 0.0035 | 0.0042 | 0.0037 | 0.0026 |
Peroxisomes | 0.0024 | 0.0001 | 0.0049 | 0.0006 | 0.0002 | 0.0002 | 0.0085 | 0.0008 | 0.0027 | 0.0013 | 0.0073 | 0.0012 | 0.0005 | 0.0005 | 0.0007 | 0.0006 | 0.0005 | 0.0003 |
Punctate Nuclear | 0.0061 | 0.0003 | 0.0028 | 0.002 | 0.0001 | 0.0017 | 0.0153 | 0.0006 | 0.0007 | 0.0004 | 0.0116 | 0.0024 | 0.0035 | 0.001 | 0.0032 | 0.0024 | 0.0038 | 0.0019 |
Vacuole | 0.0056 | 0.0038 | 0.0105 | 0.0174 | 0.0015 | 0.0043 | 0.0072 | 0.0037 | 0.0095 | 0.0084 | 0.0078 | 0.0053 | 0.0067 | 0.0056 | 0.007 | 0.0122 | 0.0121 | 0.0045 |
Vacuole Periphery | 0.0016 | 0.0004 | 0.0011 | 0.0032 | 0.0004 | 0.0008 | 0.0021 | 0.0005 | 0.0015 | 0.0023 | 0.004 | 0.0018 | 0.0008 | 0.0007 | 0.0013 | 0.0024 | 0.0019 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.9389 | 3.6614 | 8.1507 | 11.0391 | 4.7793 | 8.187 | 13.9751 | 15.8662 | 14.6729 | 11.0618 |
Translational Efficiency | 0.6391 | 1.2717 | 0.7452 | 0.6315 | 0.7743 | 0.7762 | 0.553 | 0.4591 | 0.6091 | 0.5935 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1760 | 2314 | 1130 | 1706 | 976 | 2315 | 1850 | 971 | 2736 | 4629 | 2980 | 2677 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 760.73 | 914.76 | 1001.84 | 925.63 | 754.04 | 824.29 | 841.72 | 913.78 | 758.34 | 869.52 | 902.44 | 921.33 |
Standard Deviation | 93.60 | 116.35 | 131.11 | 137.86 | 125.66 | 232.39 | 97.41 | 180.67 | 106.20 | 189.27 | 135.81 | 154.86 |
Intensity Change Log 2 | — | 0.266009 | 0.397196 | 0.283051 | — | 0.128511 | 0.158699 | 0.277206 | — | 0.199201 | 0.283396 | 0.280144 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000169 | 0.000119 | 0.000300 | 0.000929 | 0.000152 | 0.000270 | 0.000139 | 0.000383 | 0.000163 | 0.000195 | 0.000200 | 0.000731 |
Bud Neck | 0.018829 | 0.007385 | 0.006089 | 0.007391 | 0.008770 | 0.010744 | 0.008581 | 0.006957 | 0.015241 | 0.009065 | 0.007636 | 0.007234 |
Bud Site | 0.012825 | 0.014987 | 0.014802 | 0.052752 | 0.013501 | 0.019827 | 0.010246 | 0.060476 | 0.013066 | 0.017407 | 0.011973 | 0.055554 |
Cell Periphery | 0.000147 | 0.000035 | 0.000074 | 0.000083 | 0.000138 | 0.000084 | 0.000090 | 0.000157 | 0.000144 | 0.000059 | 0.000084 | 0.000110 |
Cytoplasm | 0.486911* | 0.620301* | 0.602920* | 0.567483* | 0.540330* | 0.501706* | 0.634477* | 0.346501* | 0.505967* | 0.560990* | 0.622511* | 0.487329* |
Cytoplasmic Foci | 0.249291* | 0.162431 | 0.079383 | 0.091352 | 0.225413* | 0.253354* | 0.049692 | 0.112853 | 0.240773* | 0.207902* | 0.060951 | 0.099151 |
Eisosomes | 0.000142 | 0.000017 | 0.000021 | 0.000026 | 0.000068 | 0.000054 | 0.000036 | 0.000015 | 0.000115 | 0.000036 | 0.000030 | 0.000022 |
Endoplasmic Reticulum | 0.001384 | 0.000273 | 0.001401 | 0.000495 | 0.001913 | 0.000474 | 0.000832 | 0.000475 | 0.001573 | 0.000373 | 0.001048 | 0.000488 |
Endosome | 0.068508 | 0.042031 | 0.026658 | 0.022091 | 0.049726 | 0.058697 | 0.014767 | 0.130230 | 0.061808 | 0.050366 | 0.019276 | 0.061315 |
Golgi | 0.008368 | 0.009011 | 0.000584 | 0.003302 | 0.004703 | 0.014348 | 0.000415 | 0.003258 | 0.007061 | 0.011680 | 0.000479 | 0.003287 |
Lipid Particles | 0.005353 | 0.001577 | 0.002182 | 0.001820 | 0.004463 | 0.002819 | 0.001572 | 0.001972 | 0.005036 | 0.002198 | 0.001804 | 0.001875 |
Mitochondria | 0.001534 | 0.002304 | 0.000231 | 0.001751 | 0.001076 | 0.002696 | 0.000296 | 0.001024 | 0.001371 | 0.002500 | 0.000271 | 0.001487 |
Mitotic Spindle | 0.000933 | 0.009713 | 0.032692 | 0.037264 | 0.000913 | 0.003825 | 0.004254 | 0.025566 | 0.000926 | 0.006768 | 0.015037 | 0.033021 |
None | 0.001921 | 0.000558 | 0.002851 | 0.001916 | 0.002452 | 0.001150 | 0.003951 | 0.005350 | 0.002110 | 0.000854 | 0.003534 | 0.003161 |
Nuclear Periphery | 0.000071 | 0.000137 | 0.000958 | 0.000393 | 0.000220 | 0.000069 | 0.000586 | 0.000258 | 0.000124 | 0.000103 | 0.000727 | 0.000344 |
Nuclear Periphery Foci | 0.000393 | 0.000228 | 0.009892 | 0.001451 | 0.001645 | 0.000338 | 0.001715 | 0.002290 | 0.000840 | 0.000283 | 0.004815 | 0.001755 |
Nucleolus | 0.000727 | 0.000380 | 0.000912 | 0.000292 | 0.001244 | 0.000472 | 0.000854 | 0.000792 | 0.000911 | 0.000426 | 0.000876 | 0.000474 |
Nucleus | 0.014448 | 0.008240 | 0.087910 | 0.035859 | 0.027200 | 0.010946 | 0.121754 | 0.007679 | 0.018997 | 0.009593 | 0.108921 | 0.025638 |
Peroxisomes | 0.004483 | 0.005362 | 0.005697 | 0.011388 | 0.003042 | 0.006522 | 0.001797 | 0.009102 | 0.003969 | 0.005942 | 0.003276 | 0.010559 |
Vacuole | 0.119969 | 0.110705 | 0.124134 | 0.161428 | 0.109512 | 0.107792 | 0.143051 | 0.281678* | 0.116239 | 0.109248 | 0.135878 | 0.205045* |
Vacuole Periphery | 0.003593 | 0.004207 | 0.000309 | 0.000531 | 0.003515 | 0.003815 | 0.000895 | 0.002984 | 0.003566 | 0.004011 | 0.000673 | 0.001421 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.08 | -5.55 | -3.35 | -3.88 | -1.58 | -2.45 | 0.09 | -2.51 | -1.09 | -2.45 | -1.12 | -2.57 | -4.12 | -3.57 | -3.20 |
Bud Neck | 8.88 | 7.00 | 8.75 | -0.33 | 1.55 | -1.66 | -0.31 | 1.71 | 5.06 | 2.20 | 6.59 | 5.63 | 8.55 | 3.40 | 2.40 |
Bud Site | -1.90 | -1.93 | -6.39 | -5.41 | -4.65 | -4.36 | 2.62 | -9.35 | -7.89 | -10.17 | -4.96 | 1.26 | -11.14 | -9.05 | -11.18 |
Cell Periphery | 18.19* | 9.08 | 12.83 | -9.55 | 3.92 | 3.83 | 5.17 | 0.37 | -0.98 | -1.22 | 11.75 | 9.57 | 3.99 | -1.66 | -0.42 |
Cytoplasm | -14.83 | -0.52 | 0.28 | 13.82 | 0.72 | 3.61 | -4.60 | 13.94 | 12.50 | 19.48 | -7.86 | -6.29 | 8.66 | 16.24 | 14.18 |
Cytoplasmic Foci | 14.53 | 27.20 | 28.59 | 15.35 | -0.47 | -3.84 | 26.03 | 15.71 | 22.50 | -8.65 | 6.95 | 40.67 | 32.27 | 28.20 | -6.74 |
Eisosomes | 15.48 | 14.35 | 14.32 | -6.82 | -0.44 | 2.85 | 7.98 | 13.74 | 10.26 | 12.75 | 14.13 | 15.30 | 17.27 | 6.29 | 8.35 |
Endoplasmic Reticulum | 4.75 | -1.35 | 3.73 | -3.89 | 8.86 | 4.17 | 3.14 | 4.40 | 0.74 | 3.20 | 6.15 | 2.00 | 5.68 | -1.88 | 7.56 |
Endosome | 7.66 | 12.69 | 16.72 | 11.65 | 3.56 | -2.36 | 10.16 | -9.09 | -8.16 | -16.04 | 4.42 | 17.54 | 4.05 | 0.46 | -11.73 |
Golgi | -0.75 | 17.64 | 11.54 | 12.29 | -1.33 | -10.76 | 9.40 | 3.09 | 12.23 | -3.60 | -8.08 | 19.46 | 11.37 | 17.44 | -3.26 |
Lipid Particles | 12.53 | 6.32 | 7.06 | -3.54 | 1.71 | 3.57 | 6.13 | 4.88 | 1.70 | -1.75 | 10.72 | 10.00 | 8.60 | -1.20 | -0.89 |
Mitochondria | -1.48 | 5.08 | 2.18 | 3.04 | -1.53 | -4.00 | 3.20 | 0.67 | 3.87 | -1.29 | -3.43 | 5.98 | 2.19 | 4.98 | -2.00 |
Mitotic Spindle | -5.78 | -10.77 | -11.21 | -7.39 | 2.75 | -2.86 | -4.04 | -8.24 | -7.42 | -6.67 | -6.43 | -11.29 | -13.91 | -10.84 | -3.83 |
None | 3.31 | -1.62 | 0.38 | -6.70 | 3.68 | 2.34 | -0.89 | -1.09 | -2.09 | -0.52 | 3.70 | -1.64 | -0.78 | -3.33 | 0.65 |
Nuclear Periphery | -0.88 | -17.35 | -19.66 | -9.33 | 8.03 | 3.95 | -9.37 | -2.94 | -7.75 | 6.62 | 0.46 | -20.64 | -17.86 | -12.87 | 7.39 |
Nuclear Periphery Foci | 2.38 | -10.78 | -10.95 | -11.40 | 9.24 | 5.80 | -2.85 | -2.89 | -5.24 | -1.07 | 6.02 | -11.57 | -7.28 | -9.81 | 7.45 |
Nucleolus | 1.06 | -2.38 | -0.10 | -5.01 | 3.93 | 3.37 | -0.64 | 1.06 | -2.11 | 1.87 | 2.12 | -2.32 | 0.37 | -4.11 | 4.39 |
Nucleus | 8.97 | -20.31 | -16.39 | -20.35 | 12.00 | 5.46 | -19.98 | 6.73 | 2.64 | 31.11 | 7.76 | -31.93 | -7.54 | -16.23 | 26.97 |
Peroxisomes | -1.75 | -0.15 | -3.52 | -2.21 | -2.74 | -5.83 | 1.04 | -5.35 | -1.34 | -6.25 | -5.21 | 1.75 | -5.93 | -2.30 | -6.80 |
Vacuole | 1.60 | -10.72 | -23.44 | -25.14 | -8.97 | 0.27 | -11.07 | -19.58 | -20.88 | -12.49 | 1.71 | -15.34 | -30.51 | -32.95 | -16.20 |
Vacuole Periphery | -1.62 | 13.48 | 13.50 | 17.22 | 1.07 | -0.61 | 5.42 | 2.11 | 4.27 | -4.55 | -1.51 | 10.47 | 10.53 | 16.78 | -2.47 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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