Standard name
Human Ortholog
Description Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 1.0 0.95 0.92 0.9 0.78 0.77 – 0.69 0.7 0.73 0.97 0.97 0.98 0.97 0.96 0.95 0.96 0.97 0.97 0.88 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.13 0.13 – 0.13 0.27 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.06 0 0.11 0.11 – 0.16 0.1 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 1 2 1 – 1 3 4 1 0 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 0 0 2 4 1 1 11 – 13 7 11 3 3 5 9 11 7 0 0 0 0 0 0
Cytoplasm 382 316 248 242 295 323 331 331 – 251 175 267 251 309 322 250 265 228 366 302 234 163 244 290
Endoplasmic Reticulum 3 3 1 1 1 5 9 4 – 20 5 7 2 7 6 5 5 6 1 0 1 4 3 1
Endosome 0 0 0 0 1 0 0 1 – 0 0 0 0 0 0 1 0 0 2 1 0 2 1 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0
Mitochondria 0 0 0 1 7 21 57 55 – 47 67 68 0 0 0 4 2 1 0 0 0 3 1 3
Nucleus 11 1 0 1 2 6 6 16 – 7 2 7 2 1 4 2 0 5 1 2 0 0 1 1
Nuclear Periphery 1 0 0 0 1 1 0 0 – 1 0 0 0 0 0 0 1 0 1 0 0 2 0 0
Nucleolus 0 0 0 0 0 0 0 1 – 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 0 13 19 16 48 46 – 60 25 26 3 0 2 3 13 4 3 1 0 3 4 4
Unique Cell Count 391 319 249 254 320 359 424 431 365 250 364 258 317 329 259 276 239 383 312 242 186 264 305
Labelled Cell Count 404 321 249 260 330 374 454 466 402 285 392 262 320 339 275 297 252 383 312 242 186 264 305


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.0 12.2 9.9 9.5 9.0 8.7 8.2 8.7 – 8.6 8.6 8.8 11.8 11.1 11.3 12.8 13.2 12.7 11.8 11.9 12.3
Std Deviation (1e-4) 1.8 1.8 1.4 1.3 1.7 1.4 1.5 1.5 – 1.6 1.9 1.8 1.6 1.7 1.8 2.5 2.2 2.3 1.9 1.6 1.9
Intensity Change (Log2) – – – -0.05 -0.14 -0.19 -0.26 -0.18 – -0.21 -0.2 -0.17 0.25 0.16 0.19 0.37 0.42 0.36 0.25 0.27 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 3.0 0 0 0 0 0 0 3.2 0
Cytoplasm -3.1 -4.2 -4.9 -7.8 -8.1 – -9.6 -9.2 -8.7 -2.1 -2.0 -1.8 -2.5 -2.7 -3.0
Endoplasmic Reticulum 0 0 0 0 0 – 3.4 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 6.0 5.9 – 5.9 8.8 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 3.1 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 3.6 3.9 3.4 5.5 5.3 – 6.7 5.1 4.3 0 0 0 0 3.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2436 8.1562 7.7581 6.5507 6.298 7.3455 8.7565 10.9206 9.8641 8.7253 8.6963 9.6342 5.3492 7.9886 7.3147 7.8498 6.4301 7.04
Actin 0.0197 0.0004 0.0079 0.003 0.0046 0.0086 0.0238 0.0005 0.0076 0.0114 0.0004 0.0024 0.0222 0.0002 0.0113 0.0003 0.0137 0.0031
Bud 0.0011 0.0002 0.0005 0.0005 0.0004 0.0039 0.0001 0 0.0001 0.037 0 0.0007 0.0009 0 0.0003 0.0001 0.0003 0.0002
Bud Neck 0.0049 0.0002 0.0009 0.0016 0.0006 0.0015 0.0002 0.0001 0.0006 0.0006 0.0004 0.0008 0.0023 0.0001 0.0003 0.0005 0.0007 0.0012
Bud Periphery 0.0006 0 0.0003 0.0003 0.0003 0.0018 0 0 0.0001 0.0185 0 0.0002 0.0008 0 0.0004 0 0.0003 0.0001
Bud Site 0.0029 0.0005 0.0028 0.003 0.0006 0.0004 0.0002 0.0001 0.0007 0.0012 0.0001 0.0001 0.0191 0.0001 0.0003 0 0.0006 0.0001
Cell Periphery 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0 0 0.0003 0 0 0 0.0001 0
Cytoplasm 0.7864 0.9732 0.9044 0.8917 0.7579 0.8959 0.9267 0.9882 0.9422 0.8889 0.9538 0.9579 0.861 0.9793 0.9396 0.9915 0.7657 0.9388
Cytoplasmic Foci 0.021 0.0029 0.0191 0.0152 0.028 0.0099 0.0021 0.0009 0.0121 0.0044 0.0033 0.0078 0.0289 0.0009 0.0023 0.0007 0.0142 0.0021
Eisosomes 0.0001 0 0 0.0004 0.0001 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0138 0.0017 0.0014 0.0051 0.0025 0.0035 0.0019 0.0014 0.0014 0.0009 0.002 0.0023 0.0028 0.0004 0.0012 0.0006 0.0014 0.0023
Endosome 0.0297 0.0012 0.0152 0.0091 0.044 0.017 0.0014 0.0003 0.0039 0.0006 0.0017 0.006 0.0061 0.0001 0.0149 0.0001 0.0335 0.0035
Golgi 0.0056 0.0001 0.0066 0.0011 0.0134 0.006 0.0004 0.0001 0.0024 0.0002 0.0001 0.0008 0.0035 0 0.0057 0 0.0206 0.0002
Lipid Particles 0.0099 0 0.0023 0.0056 0.0469 0.0021 0.0002 0 0.0007 0 0.0001 0.0002 0.0184 0 0.0007 0 0.0648 0.0001
Mitochondria 0.0061 0.0002 0.003 0.0049 0.024 0.0083 0.0002 0 0.0004 0.0009 0.0001 0.0009 0.001 0 0.0103 0 0.0106 0.0011
None 0.0178 0.0055 0.0046 0.0191 0.0127 0.0049 0.0104 0.0035 0.0106 0.0077 0.0302 0.003 0.0062 0.0158 0.0049 0.0011 0.0028 0.0024
Nuclear Periphery 0.0229 0.0017 0.0017 0.0067 0.0075 0.0053 0.0021 0.0005 0.001 0.0022 0.0014 0.0043 0.0016 0.0002 0.0009 0.0004 0.0233 0.0118
Nucleolus 0.0004 0 0.0001 0.0009 0.0005 0.0001 0 0 0.0007 0.0001 0 0.0001 0.0002 0 0 0 0.0004 0.0003
Nucleus 0.0308 0.0108 0.0202 0.0214 0.0122 0.0245 0.0283 0.0041 0.0084 0.0217 0.0048 0.0101 0.0065 0.0025 0.0041 0.0043 0.0127 0.0285
Peroxisomes 0.0154 0.0001 0.0051 0.0016 0.0199 0.0015 0.0007 0 0.0016 0.0005 0.0001 0.0003 0.0155 0 0.0004 0 0.015 0.0001
Punctate Nuclear 0.0063 0.0006 0.0017 0.007 0.0196 0.0031 0.0008 0.0001 0.005 0.0026 0.001 0.0012 0.0017 0.0001 0.0003 0.0002 0.0171 0.003
Vacuole 0.0027 0.0006 0.0015 0.0014 0.0019 0.0009 0.0002 0.0001 0.0003 0.0006 0.0003 0.0005 0.0007 0.0001 0.0011 0.0001 0.0007 0.0005
Vacuole Periphery 0.0016 0.0001 0.0005 0.0005 0.0023 0.0007 0 0 0.0001 0.0002 0.0001 0.0004 0.0002 0 0.0008 0 0.0015 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.9673 23.5905 18.5992 22.6712 36.3534 31.32 24.5977 25.1542 26.5232 34.1102
Translational Efficiency 2.3821 1.1086 1.5073 1.2643 1.1071 1.6307 1.4437 1.6456 1.43 1.3725

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1538 897 1730 1747 1812 1759 2511 1839 3350 2656 4241 3586

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 844.35 1099.98 1235.17 1377.90 959.46 1076.49 1266.30 1323.68 906.61 1084.42 1253.60 1350.09
Standard Deviation 127.93 186.54 178.53 242.23 209.57 155.23 181.09 214.13 185.90 166.83 180.70 229.86
Intensity Change Log 2 — 0.381564 0.548797 0.706558 — 0.166040 0.400325 0.464260 — 0.270943 0.471729 0.582756

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000235 0.000368 0.000167 0.003588 0.000135 0.000543 0.000103 0.001257 0.000181 0.000484 0.000129 0.002392
Bud Neck 0.002478 0.001493 0.000841 0.005219 0.001050 0.001522 0.002095 0.003177 0.001706 0.001512 0.001584 0.004172
Bud Site 0.013106 0.006172 0.001539 0.028469 0.003635 0.007301 0.005588 0.031791 0.007983 0.006920 0.003936 0.030172
Cell Periphery 0.000146 0.000078 0.000057 0.000090 0.000087 0.000048 0.000042 0.000130 0.000114 0.000058 0.000048 0.000110
Cytoplasm 0.744197* 0.802781* 0.573727* 0.596218* 0.788266* 0.822470* 0.759176* 0.621831* 0.768034* 0.815820* 0.683527* 0.609353*
Cytoplasmic Foci 0.138972 0.035408 0.002462 0.010506 0.035206 0.045559 0.003516 0.019074 0.082846 0.042131 0.003086 0.014900
Eisosomes 0.000024 0.000011 0.000003 0.000011 0.000014 0.000010 0.000003 0.000016 0.000019 0.000010 0.000003 0.000013
Endoplasmic Reticulum 0.002082 0.000866 0.000363 0.000791 0.005282 0.000389 0.000189 0.001739 0.003813 0.000550 0.000260 0.001277
Endosome 0.004004 0.002105 0.000198 0.005414 0.001790 0.003429 0.000750 0.016159 0.002807 0.002982 0.000525 0.010924
Golgi 0.002547 0.000262 0.000083 0.009876 0.000708 0.002889 0.000263 0.015343 0.001552 0.002002 0.000189 0.012679
Lipid Particles 0.001298 0.000517 0.000190 0.000730 0.000707 0.000450 0.000236 0.000872 0.000978 0.000472 0.000217 0.000803
Mitochondria 0.001080 0.000202 0.000275 0.003037 0.000901 0.000376 0.000491 0.011401 0.000983 0.000317 0.000403 0.007326
Mitotic Spindle 0.001730 0.003125 0.003658 0.098405 0.001839 0.008476 0.016303 0.085759 0.001789 0.006668 0.011145 0.091920
None 0.003168 0.004876 0.011024 0.010933 0.016462 0.003899 0.010604 0.009460 0.010359 0.004229 0.010775 0.010177
Nuclear Periphery 0.000101 0.000177 0.000329 0.000485 0.000346 0.000157 0.000325 0.000733 0.000234 0.000164 0.000327 0.000612
Nuclear Periphery Foci 0.001427 0.003730 0.000799 0.002341 0.002734 0.001516 0.000656 0.001976 0.002134 0.002264 0.000715 0.002154
Nucleolus 0.000291 0.000322 0.000102 0.000374 0.001197 0.000279 0.000127 0.000267 0.000781 0.000294 0.000117 0.000319
Nucleus 0.051445 0.095615 0.392753* 0.162389* 0.110703 0.056119 0.178302* 0.086037 0.083497 0.069458 0.265781* 0.123234
Peroxisomes 0.000988 0.000417 0.000022 0.001053 0.000336 0.000950 0.000124 0.001059 0.000635 0.000770 0.000082 0.001056
Vacuole 0.029202 0.040779 0.011354 0.059165 0.027772 0.043008 0.020865 0.081137 0.028429 0.042255 0.016985 0.070433
Vacuole Periphery 0.001479 0.000695 0.000054 0.000908 0.000829 0.000612 0.000241 0.010785 0.001127 0.000640 0.000165 0.005973

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.31 -0.75 -1.33 -0.79 -1.26 -1.58 -0.98 -3.21 0.54 -2.88 -1.78 -1.02 -2.12 -0.17 -1.98
Bud Neck 1.82 3.08 -1.50 -2.73 -4.12 -2.29 -4.12 -5.86 -2.77 -1.30 0.57 -0.28 -4.49 -4.17 -4.09
Bud Site 4.19 8.16 3.27 -1.00 -5.36 -3.18 0.11 -4.82 -2.14 -5.00 1.25 6.57 -0.97 -2.14 -6.99
Cell Periphery 3.33 3.68 3.06 -0.91 -1.69 4.06 4.43 1.69 -2.56 -2.78 5.42 5.52 3.41 -3.47 -3.83
Cytoplasm -2.12 27.74 28.42 28.08 1.96 -2.29 17.37 25.24 27.15 11.95 -3.94 29.95 37.94 40.52 11.74
Cytoplasmic Foci 21.15 31.49 30.92 15.89 -4.87 -4.13 18.46 14.72 18.57 -8.44 14.49 33.60 31.84 24.56 -9.22
Eisosomes 5.73 13.87 7.75 0.36 -13.85 2.05 5.67 0.52 -2.75 -13.57 5.81 13.03 5.24 -1.92 -19.35
Endoplasmic Reticulum 2.96 4.02 4.18 3.66 0.86 3.31 3.40 3.14 -1.23 -1.99 4.00 4.25 4.01 0.20 -1.82
Endosome 4.87 14.36 8.46 2.55 -2.96 -3.04 6.83 -2.46 -0.15 -4.95 0.16 14.95 1.92 1.44 -5.63
Golgi 9.56 10.24 2.48 -1.70 -1.98 -2.38 3.12 -2.79 -0.30 -3.70 -0.25 9.61 -0.83 -0.41 -4.16
Lipid Particles 2.79 3.95 0.47 -6.16 -13.09 1.81 1.40 -1.37 -3.40 -3.82 3.44 4.10 -0.36 -6.40 -10.68
Mitochondria 2.42 1.96 0.54 -2.79 -2.11 1.35 1.24 -1.85 -3.01 -2.95 2.50 2.20 -1.34 -3.71 -3.48
Mitotic Spindle -1.34 -3.68 -10.98 -9.94 -9.38 -3.62 -6.77 -9.76 -7.21 -6.18 -3.93 -7.76 -14.65 -11.58 -10.67
None -1.82 -12.13 -10.74 -8.79 -0.44 6.12 2.99 4.64 -4.13 5.60 5.35 -0.12 1.27 -8.78 3.69
Nuclear Periphery -5.84 -18.63 -29.23 -22.34 -14.49 1.92 -4.78 -5.96 -13.42 -2.59 0.37 -10.25 -17.71 -24.67 -12.01
Nuclear Periphery Foci -5.88 1.63 -0.15 5.67 -1.53 2.96 5.84 3.91 0.10 -5.39 -0.67 6.11 3.40 3.49 -4.46
Nucleolus -0.30 1.88 -0.54 0.13 -4.13 3.18 3.58 3.49 0.72 -1.07 2.67 3.87 3.28 0.36 -4.09
Nucleus -11.93 -63.80 -35.16 -17.75 31.96 13.97 -23.95 -4.53 -20.69 20.58 2.83 -56.41 -26.18 -29.40 32.48
Peroxisomes 2.29 4.76 3.92 1.76 -2.29 -2.25 2.25 0.85 2.90 -3.08 -0.51 5.17 3.77 3.38 -3.52
Vacuole -8.47 -0.58 -30.18 -20.28 -29.46 -9.55 -13.37 -30.20 -23.56 -22.29 -12.95 -11.89 -42.66 -31.62 -35.33
Vacuole Periphery 2.29 5.06 4.61 1.29 -2.45 0.77 2.41 -1.90 -2.50 -3.47 2.22 5.09 0.14 -1.68 -3.64
Standard name
Human Ortholog
Description Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ypt32

Ypt32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypt32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available