Standard name
Human Ortholog
Description Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.39 0.36 0.49 0.37 0.54 0.46 0.43 0.38 0.38 0.44 0.41 0.26 0.39 0.45 0.55 0.5 0.45 0.38 0.07 0.05 0.09 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.35 0.53 0.46 0.42 0.26 0.29 0.25 0.26 0.35 0.15 0.2 0.46 0.43 0.41 0.33 0.37 0.42 0.27 0.59 0.62 0.55 0.64 0.59
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.18 0.25 0.3 0.34 0.23 0.38 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.08 0 0 0.05 0 0.06 0 0 0 0.13 0.13 0.09 0.05 0 0.06 0 0 0.05 0.06 0.06 0.09
Vac/Vac Membrane 0.14 0.46 0.31 0.18 0.23 0.09 0.09 0.11 0.16 0.16 0.12 0.13 0.46 0.33 0.25 0.3 0.33 0.38 0.23 0.24 0.22 0.24 0.17 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 11 2 0 0 2 1 1 1 0 1 4 0 3 5 7 4 3 1 9 2 1 1 0
Bud 3 5 13 1 2 15 13 23 12 25 18 20 1 2 3 0 1 0 1 8 3 5 3 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 1
Bud Site 0 0 0 0 0 1 0 1 2 3 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 1 1 0 1 0 3 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 82 259 124 78 74 212 202 208 151 221 208 226 94 200 230 224 115 108 54 50 17 29 19 36
Endoplasmic Reticulum 2 15 7 1 6 11 7 6 5 1 8 10 3 0 2 14 7 6 1 3 0 0 4 7
Endosome 24 236 181 72 84 101 129 124 106 207 72 111 164 220 205 133 85 101 38 432 224 186 230 289
Golgi 0 3 0 0 1 0 1 3 0 0 2 0 1 0 1 8 3 1 1 5 1 0 0 5
Mitochondria 3 3 4 4 19 69 111 144 138 132 180 187 0 0 0 10 5 1 2 5 2 5 5 2
Nucleus 0 1 0 0 1 2 1 2 0 0 0 1 0 1 0 0 0 1 0 5 0 2 2 1
Nuclear Periphery 1 1 1 0 0 0 0 0 0 1 2 4 0 0 0 0 0 1 1 7 6 2 4 8
Nucleolus 3 17 18 4 2 8 12 19 9 21 20 10 16 18 26 11 8 7 0 2 0 1 0 1
Peroxisomes 0 0 1 4 0 0 5 1 6 2 1 3 3 0 0 0 1 0 0 0 0 0 0 1
SpindlePole 5 25 21 13 3 10 22 20 23 24 8 12 46 69 47 19 9 14 3 23 18 21 21 44
Vac/Vac Membrane 17 305 107 28 47 37 39 56 65 94 59 71 163 171 129 121 75 91 32 173 77 79 60 79
Unique Cell Count 124 668 344 158 201 391 439 487 401 585 473 546 356 514 506 406 230 242 144 734 360 339 361 493
Labelled Cell Count 142 881 479 205 239 469 544 609 518 732 579 663 491 684 649 547 313 334 144 734 360 339 361 493


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.6 7.2 7.0 6.5 6.4 5.6 6.0 6.1 5.9 5.8 6.0 8.8 8.7 8.8 11.4 10.7 10.8 9.4 9.6 9.8
Std Deviation (1e-4) 2.4 1.1 1.0 1.2 1.2 1.0 0.9 1.0 1.3 1.1 1.1 1.1 1.1 1.1 1.0 2.0 2.1 1.7 1.3 1.4 1.5
Intensity Change (Log2) – – – -0.04 -0.15 -0.18 -0.37 -0.26 -0.25 -0.29 -0.31 -0.28 0.28 0.27 0.28 0.65 0.57 0.58 0.38 0.4 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.9 0 -0.6 0.7 -0.6 0.4 0 -0.1 -3.3 -3.7 -3.4 -4.0 -2.5 -3.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 0.2 4.9 2.8 1.9 0.5 0.5 2.3 1.6 -2.8 0.8 2.7 5.2 3.3 2.1
Endoplasmic Reticulum 0 0 0.7 -0.5 -0.9 -0.9 0 -0.4 -0.2 0 0 0 1.2 0.8 0.4
Endosome -1.5 -2.4 -7.5 -6.6 -8.0 -7.3 -5.1 -11.4 -10.0 -1.7 -2.8 -3.5 -5.5 -3.7 -2.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.6 7.5 9.5 10.5 11.5 8.9 12.5 11.7 0 0 0 1.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.4 -2.5 -2.3 -1.8 -0.9 -2.2 -1.2 -0.7 -2.8 -0.5 -1.2 -0.1 -1.8 -1.0 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.9 -2.5 -2.4 -0.7 -1.3 -0.2 -1.4 -3.4 -3.0 3.1 3.4 1.7 -0.9 -1.2 -0.2
Vacuole -3.1 -1.9 -7.4 -7.9 -7.0 -4.8 -5.4 -6.5 -6.6 4.0 0.7 -1.8 -0.4 0.4 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1686 4.0149 3.7482 3.7297 3.3287 3.6133 5.9904 6.756 6.8184 6.36 6.5941 6.7711 3.8426 5.0096 4.6721 4.7037 4.0827 4.3499
Actin 0.0038 0.0002 0.0002 0.0005 0.0002 0.0002 0.0048 0.0002 0.0002 0.0007 0 0.0004 0.0212 0.0001 0.0145 0.0073 0.0026 0.004
Bud 0.0072 0.0088 0.0049 0.0151 0.0007 0.0012 0.0003 0.001 0.0007 0.0037 0.0005 0.0004 0.0007 0.0004 0.0005 0.0054 0.0009 0.0005
Bud Neck 0.0022 0.0011 0.0015 0.0004 0.0012 0.0034 0.0006 0.0012 0.0009 0.0004 0.0007 0.0012 0.0031 0.001 0.0015 0.0013 0.0008 0.0014
Bud Periphery 0.0029 0.0011 0.0016 0.0054 0.0002 0.0005 0.0001 0.0004 0.0005 0.0014 0.0002 0.0003 0.0009 0.0002 0.0003 0.0038 0.0017 0.0004
Bud Site 0.0139 0.0065 0.005 0.0018 0.0008 0.0026 0.0009 0.0049 0.0014 0.0009 0.0026 0.0007 0.0049 0.0035 0.0038 0.0063 0.0023 0.0007
Cell Periphery 0.0003 0.0001 0.0002 0.0003 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001
Cytoplasm 0.2626 0.3255 0.3033 0.2027 0.1679 0.2782 0.1694 0.2967 0.2316 0.2492 0.1217 0.1616 0.1294 0.2854 0.2327 0.2282 0.1304 0.1986
Cytoplasmic Foci 0.1816 0.1855 0.2124 0.2217 0.2639 0.2281 0.1981 0.1999 0.2319 0.2809 0.2178 0.2729 0.1784 0.145 0.1571 0.1786 0.2067 0.1982
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0042 0.0035 0.0031 0.0016 0.0015 0.0028 0.0042 0.0036 0.003 0.0024 0.0049 0.0033 0.0041 0.0038 0.0032 0.006 0.0033 0.0041
Endosome 0.3589 0.3294 0.3417 0.3949 0.4253 0.3473 0.5103 0.375 0.439 0.4074 0.53 0.4395 0.4232 0.3526 0.4361 0.3663 0.4461 0.4133
Golgi 0.0072 0.008 0.0101 0.0072 0.0114 0.0076 0.0115 0.0106 0.0133 0.0151 0.0102 0.0149 0.0238 0.0096 0.0125 0.0064 0.0098 0.0107
Lipid Particles 0.01 0.0099 0.0058 0.0047 0.0069 0.0049 0.0082 0.0057 0.0101 0.0064 0.0027 0.0072 0.0551 0.0226 0.0118 0.0024 0.0097 0.0088
Mitochondria 0.0013 0.0014 0.0023 0.0021 0.001 0.0014 0.0008 0.0023 0.0011 0.0012 0.0031 0.0026 0.0104 0.0018 0.0012 0.0025 0.0103 0.0089
None 0.0467 0.0135 0.0184 0.0688 0.0093 0.0224 0.0073 0.0131 0.0106 0.0056 0.0012 0.017 0.0154 0.0091 0.0028 0.006 0.0022 0.0022
Nuclear Periphery 0.0057 0.0088 0.0076 0.0045 0.0043 0.0058 0.0039 0.0034 0.0054 0.0009 0.0025 0.0035 0.0048 0.0041 0.0035 0.0217 0.0347 0.0106
Nucleolus 0.0011 0.0035 0.0044 0.0021 0.0009 0.0017 0.0003 0.0011 0.0005 0.0001 0.0002 0.0005 0.0018 0.0052 0.0032 0.0007 0.0067 0.001
Nucleus 0.015 0.014 0.019 0.0076 0.0089 0.0151 0.0082 0.0074 0.0047 0.0011 0.0016 0.0059 0.0073 0.0097 0.0166 0.0119 0.017 0.0171
Peroxisomes 0.0104 0.0071 0.0095 0.007 0.0111 0.0075 0.0051 0.0053 0.0096 0.0074 0.0273 0.012 0.0146 0.0035 0.008 0.0023 0.0023 0.0037
Punctate Nuclear 0.0125 0.0072 0.0132 0.0093 0.0202 0.0138 0.0076 0.0107 0.004 0.001 0.0349 0.0082 0.0134 0.0132 0.0056 0.0179 0.0075 0.0057
Vacuole 0.0464 0.0584 0.0305 0.0366 0.0548 0.0458 0.052 0.05 0.026 0.0109 0.0296 0.0357 0.0783 0.1176 0.0755 0.108 0.0928 0.0923
Vacuole Periphery 0.0063 0.0064 0.0053 0.0058 0.0096 0.0095 0.0064 0.0073 0.0054 0.0033 0.008 0.0121 0.0085 0.0113 0.0093 0.017 0.012 0.0179

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7019 6.2232 8.4661 8.4433 12.0346 12.7558 10.7493 7.0781 10.1604 9.4995
Translational Efficiency 2.8595 3.6219 1.9269 2.2133 2.138 1.8574 2.3377 3.405 1.9294 2.0252

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1743 2430 469 556 1685 2181 1341 1525 3428 4611 1810 2081

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 777.02 954.94 943.15 1089.32 839.84 873.22 987.17 1029.70 807.90 916.29 975.76 1045.63
Standard Deviation 115.65 145.28 126.57 163.43 113.41 120.89 129.49 147.34 118.78 140.36 130.17 154.08
Intensity Change Log 2 — 0.297458 0.279536 0.487404 — 0.056231 0.233184 0.294038 — 0.177198 0.255645 0.390202

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000111 0.000098 0.000178 0.000274 0.000053 0.000181 0.000065 0.000186 0.000083 0.000137 0.000095 0.000209
Bud Neck 0.009562 0.013328 0.033332 0.018210 0.009275 0.013429 0.021274 0.016549 0.009421 0.013376 0.024398 0.016993
Bud Site 0.020012 0.020563 0.023478 0.037670 0.015922 0.017151 0.029118 0.027939 0.018002 0.018949 0.027656 0.030539
Cell Periphery 0.000091 0.000051 0.000074 0.000057 0.000049 0.000052 0.000036 0.000068 0.000070 0.000051 0.000046 0.000065
Cytoplasm 0.087733 0.057900 0.076492 0.052704 0.068548 0.033934 0.084106 0.042136 0.078303 0.046564 0.082133 0.044959
Cytoplasmic Foci 0.156913 0.129463 0.037784 0.066319 0.097206 0.169518 0.072408 0.056360 0.127564 0.148409 0.063436 0.059021
Eisosomes 0.000026 0.000010 0.000033 0.000011 0.000011 0.000015 0.000010 0.000013 0.000019 0.000012 0.000016 0.000012
Endoplasmic Reticulum 0.001505 0.000973 0.002662 0.000384 0.000973 0.001036 0.000536 0.000827 0.001244 0.001003 0.001087 0.000708
Endosome 0.289890* 0.388649* 0.220583* 0.223421* 0.422238* 0.480900* 0.281084* 0.289744* 0.354944* 0.432283* 0.265407* 0.272023*
Golgi 0.017976 0.014816 0.002484 0.014384 0.011958 0.015687 0.005109 0.016358 0.015018 0.015228 0.004429 0.015831
Lipid Particles 0.003351 0.001844 0.002125 0.001624 0.002436 0.003823 0.001391 0.001143 0.002901 0.002780 0.001581 0.001271
Mitochondria 0.019399 0.006668 0.001340 0.003204 0.007066 0.007042 0.001479 0.003325 0.013337 0.006845 0.001443 0.003292
Mitotic Spindle 0.005865 0.043607 0.034032 0.036415 0.004097 0.042318 0.043558 0.026541 0.004996 0.042998 0.041090 0.029179
None 0.000986 0.000516 0.002056 0.000153 0.000310 0.000797 0.000320 0.000597 0.000654 0.000649 0.000770 0.000478
Nuclear Periphery 0.000382 0.001242 0.002401 0.001251 0.000300 0.000639 0.001462 0.001298 0.000341 0.000956 0.001706 0.001286
Nuclear Periphery Foci 0.000678 0.006817 0.008050 0.004484 0.001070 0.007640 0.002753 0.004134 0.000871 0.007206 0.004126 0.004227
Nucleolus 0.001280 0.002891 0.023336 0.004221 0.000818 0.003933 0.006425 0.006000 0.001053 0.003384 0.010807 0.005525
Nucleus 0.005769 0.003101 0.066547 0.006023 0.002180 0.003189 0.009931 0.005895 0.004005 0.003143 0.024601 0.005929
Peroxisomes 0.003862 0.006379 0.005614 0.007646 0.004305 0.012481 0.007761 0.005451 0.004080 0.009265 0.007205 0.006037
Vacuole 0.289616* 0.246464* 0.429144* 0.481007* 0.298758* 0.155859 0.407270* 0.449452* 0.294110* 0.203608* 0.412938* 0.457883*
Vacuole Periphery 0.084994 0.054620 0.028256 0.040538 0.052427 0.030378 0.023902 0.045986 0.068986 0.043153 0.025030 0.044530

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.29 -3.11 -1.11 -1.17 -0.13 -1.09 -3.04 -2.70 0.01 -1.86 -0.90 -2.81 -2.41 -0.78 -1.48
Bud Neck -3.69 -9.27 -7.04 -5.17 3.19 -3.46 -11.00 -6.39 -3.33 5.25 -5.04 -14.49 -9.30 -5.66 6.10
Bud Site -0.41 -1.65 -5.25 -5.13 -3.72 -0.94 -7.12 -5.97 -5.23 0.68 -1.00 -6.67 -7.41 -6.87 -1.33
Cell Periphery 1.89 0.84 1.64 -0.86 1.83 -0.33 2.15 -1.52 -1.18 -2.59 1.70 2.02 0.65 -1.42 -1.91
Cytoplasm 6.24 -1.15 0.62 -3.93 1.47 7.84 -7.01 2.47 -5.69 9.55 9.69 -6.08 3.78 -4.96 8.93
Cytoplasmic Foci 5.01 25.89 19.01 15.37 -4.39 -14.56 8.91 11.84 24.86 3.01 -6.24 22.57 23.34 29.39 0.82
Eisosomes 3.35 -1.56 3.03 -1.46 9.43 -3.05 -0.93 -1.20 2.02 -0.44 2.66 0.39 2.65 0.05 6.22
Endoplasmic Reticulum 2.35 -5.37 9.79 2.38 9.68 -0.14 1.09 0.52 0.61 -0.58 1.10 -0.28 2.73 1.23 3.57
Endosome -11.06 9.28 11.51 21.04 0.68 -5.54 21.33 16.78 23.62 -5.05 -11.05 20.29 16.49 27.82 -4.09
Golgi 2.69 15.52 4.14 2.53 -4.94 -3.00 8.71 -1.41 0.73 -7.20 -0.03 16.60 1.37 1.46 -8.52
Lipid Particles 3.76 2.27 3.02 -1.15 0.64 -2.44 1.24 3.13 6.06 5.78 0.33 2.99 5.19 5.89 4.71
Mitochondria 6.73 10.06 8.28 3.46 -1.12 0.09 5.94 4.01 4.20 -4.08 5.75 11.53 9.57 5.90 -3.68
Mitotic Spindle -13.46* -4.99 -7.33 -0.04 -1.26 -13.38* -12.32 -8.58 3.71 5.40 -18.99 -13.15 -11.07 3.45 4.09
None 1.66 -1.58 2.38 1.58 2.98 -1.65 -0.96 -1.51 0.61 -1.08 -0.04 -0.74 0.65 0.68 1.41
Nuclear Periphery -4.69 -9.23 -7.48 -2.35 4.30 -4.29 -7.29 -4.76 -3.44 1.39 -5.93 -10.58 -6.50 -3.10 2.86
Nuclear Periphery Foci -6.86 -6.31 -5.89 1.97 2.55 -6.59 -5.44 -3.12 3.11 -0.35 -9.49 -8.84 -4.93 3.62 0.91
Nucleolus -4.49 -8.32 -5.63 -3.01 6.53 -5.84 -9.33 -6.03 -2.42 0.98 -7.40 -12.05 -7.17 -3.60 4.77
Nucleus 3.44 -10.77 -3.60 -5.60 9.80 -2.45 -11.14 -8.18 -6.49 5.80 1.75 -13.65 -7.07 -8.76 10.89
Peroxisomes -4.52 -1.94 -3.05 0.12 -1.70 -9.44 -3.10 -1.30 8.00 1.80 -10.17 -3.94 -2.71 6.00 1.12
Vacuole 5.79 -9.68 -16.15 -20.12 -4.31 18.68 -12.85 -17.22 -35.88 -4.04 16.72 -16.87 -23.88 -38.24 -5.70
Vacuole Periphery 8.57 16.42 14.18 7.67 -2.58 9.09 15.39 4.05 -4.98 -11.33 11.79 23.91 12.80 2.02 -11.13
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages vacuolar membrane (27%), endosome (7%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vam7

Vam7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vam7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available