Standard name | |
---|---|
Human Ortholog | |
Description | Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 4 | 8 | 0 | 4 | 5 | 11 | 4 | 13 | 6 | 6 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 7 | 5 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 11 | 1 | 6 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 14 | 60 | 74 | 86 | 102 | 108 | 131 | 83 | 87 | 69 | 96 | 7 | 48 | 60 | 120 | 92 | 100 | 15 | 29 | 74 | 9 | 26 | 18 |
Endoplasmic Reticulum | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 9 | 14 | 11 | 0 | 0 | 2 | 1 | 0 | 2 |
Endosome | 5 | 0 | 0 | 1 | 8 | 2 | 5 | 3 | 0 | 0 | 1 | 0 | 0 | 7 | 4 | 26 | 30 | 26 | 3 | 1 | 7 | 1 | 5 | 2 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 21 | 99 | 145 | 103 | 174 | 274 | 352 | 181 | 234 | 198 | 284 | 7 | 10 | 26 | 4 | 1 | 7 | 1 | 3 | 25 | 0 | 5 | 2 |
Nucleus | 16 | 4 | 25 | 29 | 52 | 42 | 98 | 102 | 50 | 53 | 37 | 44 | 1 | 25 | 21 | 37 | 33 | 44 | 33 | 30 | 82 | 8 | 26 | 18 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 11 | 1 | 4 | 4 |
Vac/Vac Membrane | 25 | 14 | 43 | 30 | 61 | 61 | 72 | 95 | 30 | 48 | 29 | 59 | 9 | 66 | 75 | 179 | 155 | 195 | 21 | 18 | 89 | 5 | 28 | 28 |
Unique Cell Count | 47 | 45 | 208 | 228 | 269 | 314 | 441 | 543 | 266 | 333 | 266 | 375 | 20 | 139 | 166 | 323 | 265 | 322 | 89 | 99 | 312 | 30 | 109 | 92 |
Labelled Cell Count | 58 | 54 | 235 | 290 | 316 | 385 | 572 | 714 | 353 | 443 | 347 | 499 | 24 | 156 | 189 | 376 | 327 | 387 | 89 | 99 | 312 | 30 | 109 | 92 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 3.8 | 3.7 | 3.5 | 4.2 | 3.9 | 3.7 | 3.8 | 3.5 | 3.6 | 3.6 | 3.3 | 4.6 | 5.4 | 4.8 | 7.4 | 7.7 | 8.0 | – | 4.8 | 6.4 |
Std Deviation (1e-4) | 1.0 | 0.6 | 0.6 | 0.8 | 1.3 | 1.0 | 1.0 | 1.0 | 1.1 | 1.4 | 1.2 | 1.0 | 0.8 | 1.6 | 1.2 | 1.1 | 1.4 | 1.6 | – | 0.8 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.17 | 0.05 | 0.0 | 0.02 | -0.09 | -0.05 | -0.06 | -0.18 | 0.32 | 0.54 | 0.36 | 0.98 | 1.04 | 1.1 | – | 0.38 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 7.9 | 8.3 | 9.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0573 | 2.2562 | 2.2106 | 1.8922 | 1.9444 | 1.9046 | 1.5016 | 2.6117 | 2.2397 | 1.9465 | 2.1548 | 1.9797 | 3.5829 | 3.5078 | 2.884 | 3.5849 | 2.8282 | 3.2776 |
Actin | 0.0527 | 0.0004 | 0.0122 | 0.0252 | 0.0389 | 0.0003 | 0.0306 | 0.0005 | 0.0134 | 0.0006 | 0.0233 | 0.0006 | 0.0391 | 0.0003 | 0.0025 | 0.0002 | 0.0001 | 0.0094 |
Bud | 0.0015 | 0.0003 | 0.0004 | 0.0005 | 0.0019 | 0.0002 | 0.001 | 0.0003 | 0.0009 | 0.0001 | 0.0006 | 0.0002 | 0.0062 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0005 |
Bud Neck | 0.0055 | 0.0011 | 0.0096 | 0.0047 | 0.002 | 0.0044 | 0.0058 | 0.0008 | 0.0014 | 0.0011 | 0.0021 | 0.0041 | 0.0121 | 0.0003 | 0.0007 | 0.0006 | 0.0006 | 0.0049 |
Bud Periphery | 0.0029 | 0.0001 | 0.0003 | 0.0007 | 0.0032 | 0.0005 | 0.0013 | 0.0001 | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0023 | 0 | 0.0002 | 0.0001 | 0 | 0.0005 |
Bud Site | 0.0102 | 0.0021 | 0.0037 | 0.0022 | 0.0063 | 0.0002 | 0.0136 | 0.0024 | 0.0065 | 0.0002 | 0.0046 | 0.0003 | 0.024 | 0.0006 | 0.0011 | 0.0001 | 0.0001 | 0.0009 |
Cell Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.1579 | 0.2869 | 0.201 | 0.1447 | 0.2892 | 0.1538 | 0.1839 | 0.2612 | 0.178 | 0.1505 | 0.1489 | 0.1476 | 0.1605 | 0.3454 | 0.2545 | 0.0909 | 0.3131 | 0.2132 |
Cytoplasmic Foci | 0.0288 | 0.0048 | 0.0127 | 0.0174 | 0.0066 | 0.0027 | 0.0198 | 0.0049 | 0.0166 | 0.0022 | 0.0164 | 0.0032 | 0.0231 | 0.0012 | 0.007 | 0.0004 | 0.0007 | 0.014 |
Eisosomes | 0.0011 | 0 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0178 | 0.0086 | 0.0108 | 0.0101 | 0.0128 | 0.0061 | 0.0087 | 0.0032 | 0.0033 | 0.0052 | 0.0032 | 0.0022 | 0.0062 | 0.0047 | 0.0078 | 0.0009 | 0.0028 | 0.0049 |
Endosome | 0.0428 | 0.0054 | 0.0191 | 0.018 | 0.027 | 0.0037 | 0.0305 | 0.003 | 0.0124 | 0.003 | 0.0203 | 0.0061 | 0.0201 | 0.0024 | 0.0115 | 0.0005 | 0.0012 | 0.0119 |
Golgi | 0.0121 | 0.0002 | 0.0031 | 0.0146 | 0.0107 | 0.0002 | 0.0073 | 0.0001 | 0.0044 | 0.0001 | 0.006 | 0.0016 | 0.0093 | 0 | 0.0056 | 0 | 0 | 0.0019 |
Lipid Particles | 0.015 | 0.0003 | 0.0094 | 0.0091 | 0.0054 | 0.0003 | 0.0109 | 0.0002 | 0.0033 | 0.0002 | 0.0117 | 0.0011 | 0.0081 | 0 | 0.001 | 0 | 0 | 0.0041 |
Mitochondria | 0.0167 | 0.0159 | 0.0025 | 0.0042 | 0.0434 | 0.0081 | 0.0163 | 0.001 | 0.0057 | 0.0011 | 0.0056 | 0.0006 | 0.0037 | 0.0004 | 0.002 | 0.0003 | 0.0004 | 0.0027 |
None | 0.0614 | 0.0348 | 0.0334 | 0.0127 | 0.0642 | 0.0089 | 0.1114 | 0.1086 | 0.1097 | 0.1372 | 0.092 | 0.0536 | 0.0473 | 0.02 | 0.0912 | 0.0873 | 0.0563 | 0.0643 |
Nuclear Periphery | 0.0589 | 0.0234 | 0.0355 | 0.0218 | 0.0333 | 0.0198 | 0.0272 | 0.0188 | 0.0153 | 0.042 | 0.0322 | 0.0107 | 0.0433 | 0.0281 | 0.0354 | 0.0073 | 0.0112 | 0.0209 |
Nucleolus | 0.0066 | 0.0031 | 0.0041 | 0.0022 | 0.0017 | 0.0042 | 0.0035 | 0.0023 | 0.0025 | 0.0019 | 0.0091 | 0.0038 | 0.003 | 0.0011 | 0.0021 | 0.001 | 0.0016 | 0.0029 |
Nucleus | 0.454 | 0.577 | 0.6021 | 0.6701 | 0.4295 | 0.7613 | 0.4738 | 0.5748 | 0.5894 | 0.6163 | 0.5481 | 0.7353 | 0.5315 | 0.5861 | 0.5545 | 0.8053 | 0.6041 | 0.5863 |
Peroxisomes | 0.0137 | 0.0003 | 0.008 | 0.0203 | 0.0012 | 0 | 0.0097 | 0.0001 | 0.0051 | 0.0001 | 0.0259 | 0.0003 | 0.0071 | 0 | 0.0005 | 0 | 0 | 0.0035 |
Punctate Nuclear | 0.0196 | 0.0276 | 0.0242 | 0.0177 | 0.0118 | 0.0202 | 0.0383 | 0.0145 | 0.0278 | 0.0362 | 0.0453 | 0.0262 | 0.047 | 0.0068 | 0.0197 | 0.0044 | 0.0064 | 0.0494 |
Vacuole | 0.0145 | 0.007 | 0.0061 | 0.0026 | 0.0078 | 0.0035 | 0.0044 | 0.0026 | 0.0024 | 0.001 | 0.0021 | 0.0021 | 0.0035 | 0.0019 | 0.0017 | 0.0004 | 0.0009 | 0.0029 |
Vacuole Periphery | 0.0055 | 0.0007 | 0.0012 | 0.0008 | 0.0023 | 0.0006 | 0.0012 | 0.0004 | 0.0007 | 0.0007 | 0.0008 | 0.0003 | 0.0017 | 0.0005 | 0.0007 | 0.0001 | 0.0002 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.8623 | 5.1427 | 6.1058 | 11.5257 | 7.4589 | 11.1161 | 10.2263 | 14.1551 | 16.8539 | 12.4176 |
Translational Efficiency | 0.7227 | 0.8178 | 0.8602 | 0.9001 | 0.793 | 0.9213 | 0.7072 | 0.564 | 0.6773 | 0.5858 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1158 | 295 | 119 | 1418 | 1794 | 1288 | 486 | 62 | 2952 | 1583 | 605 | 1480 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 623.66 | 839.73 | 1151.29 | 840.13 | 683.77 | 744.88 | 1057.42 | 980.40 | 660.19 | 762.56 | 1075.88 | 846.01 |
Standard Deviation | 66.61 | 113.91 | 120.82 | 129.15 | 88.40 | 104.92 | 122.14 | 91.83 | 85.74 | 112.87 | 127.47 | 130.85 |
Intensity Change Log 2 | — | 0.429166 | 0.884420 | 0.429853 | — | 0.123497 | 0.628965 | 0.519859 | — | 0.277394 | 0.756470 | 0.477625 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000029 | 0.000353 | 0.003622 | 0.000680 | 0.000072 | 0.000154 | 0.001019 | 0.001132 | 0.000055 | 0.000191 | 0.001531 | 0.000699 |
Bud Neck | 0.002579 | 0.004894 | 0.006311 | 0.012325 | 0.003756 | 0.006114 | 0.010709 | 0.003217 | 0.003294 | 0.005887 | 0.009844 | 0.011944 |
Bud Site | 0.000529 | 0.002553 | 0.003511 | 0.017098 | 0.000728 | 0.004473 | 0.002783 | 0.001801 | 0.000650 | 0.004115 | 0.002926 | 0.016457 |
Cell Periphery | 0.000126 | 0.000114 | 0.000289 | 0.000362 | 0.000074 | 0.000110 | 0.000120 | 0.000159 | 0.000095 | 0.000111 | 0.000153 | 0.000353 |
Cytoplasm | 0.518582* | 0.236056* | 0.011955 | 0.221091* | 0.345863* | 0.328044* | 0.018877 | 0.048791 | 0.413617* | 0.310902* | 0.017515 | 0.213873* |
Cytoplasmic Foci | 0.099702 | 0.054331 | 0.001262 | 0.015478 | 0.042934 | 0.052605 | 0.000154 | 0.002332 | 0.065203 | 0.052927 | 0.000372 | 0.014927 |
Eisosomes | 0.000074 | 0.000060 | 0.000094 | 0.000062 | 0.000032 | 0.000043 | 0.000037 | 0.000083 | 0.000048 | 0.000046 | 0.000048 | 0.000062 |
Endoplasmic Reticulum | 0.000366 | 0.003207 | 0.024351 | 0.003427 | 0.001258 | 0.001708 | 0.012860 | 0.014462 | 0.000908 | 0.001987 | 0.015120 | 0.003889 |
Endosome | 0.002602 | 0.004204 | 0.001624 | 0.011737 | 0.001523 | 0.003019 | 0.000758 | 0.001365 | 0.001946 | 0.003240 | 0.000928 | 0.011303 |
Golgi | 0.000142 | 0.000827 | 0.000301 | 0.005942 | 0.000145 | 0.002153 | 0.000087 | 0.000114 | 0.000144 | 0.001906 | 0.000129 | 0.005698 |
Lipid Particles | 0.004693 | 0.004690 | 0.002063 | 0.004262 | 0.003237 | 0.004312 | 0.000462 | 0.001873 | 0.003808 | 0.004383 | 0.000777 | 0.004162 |
Mitochondria | 0.002369 | 0.001761 | 0.001438 | 0.006133 | 0.001766 | 0.003885 | 0.001290 | 0.000886 | 0.002003 | 0.003489 | 0.001319 | 0.005913 |
Mitotic Spindle | 0.000136 | 0.008601 | 0.000380 | 0.045734 | 0.000912 | 0.006194 | 0.002804 | 0.001188 | 0.000607 | 0.006643 | 0.002327 | 0.043868 |
None | 0.010359 | 0.006657 | 0.001353 | 0.017967 | 0.008367 | 0.009279 | 0.006817 | 0.003726 | 0.009149 | 0.008790 | 0.005742 | 0.017370 |
Nuclear Periphery | 0.000550 | 0.002024 | 0.001283 | 0.002104 | 0.002105 | 0.001865 | 0.002065 | 0.002427 | 0.001495 | 0.001895 | 0.001911 | 0.002118 |
Nuclear Periphery Foci | 0.000475 | 0.002832 | 0.002469 | 0.001444 | 0.000931 | 0.000886 | 0.000670 | 0.001717 | 0.000752 | 0.001249 | 0.001024 | 0.001455 |
Nucleolus | 0.001011 | 0.002752 | 0.005391 | 0.003637 | 0.002411 | 0.004524 | 0.002233 | 0.004234 | 0.001862 | 0.004194 | 0.002854 | 0.003662 |
Nucleus | 0.339335* | 0.630574* | 0.906049* | 0.594533* | 0.573706* | 0.549943* | 0.925255* | 0.859571* | 0.481768* | 0.564969* | 0.921478* | 0.605636* |
Peroxisomes | 0.000928 | 0.003590 | 0.000886 | 0.002002 | 0.001477 | 0.003498 | 0.000162 | 0.000323 | 0.001262 | 0.003515 | 0.000304 | 0.001932 |
Vacuole | 0.015205 | 0.029568 | 0.025035 | 0.030447 | 0.008460 | 0.016574 | 0.010704 | 0.050400 | 0.011106 | 0.018995 | 0.013523 | 0.031283 |
Vacuole Periphery | 0.000207 | 0.000355 | 0.000332 | 0.003537 | 0.000242 | 0.000617 | 0.000133 | 0.000199 | 0.000228 | 0.000568 | 0.000172 | 0.003397 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.97 | -10.09 | -6.30 | -3.30 | 7.50 | -3.13 | -16.18 | -9.79 | -9.22 | -1.58 | -5.82 | -16.83 | -6.59 | -5.44 | 5.78 |
Bud Neck | -2.33 | -6.48 | -10.77 | -6.30 | -5.10 | -2.36 | -7.77 | -3.44 | -1.25 | 4.59 | -3.44 | -8.98 | -10.02 | -6.70 | -0.06 |
Bud Site | -4.21 | -6.26 | -8.07 | -6.29 | -5.24 | -3.00 | -5.30 | -6.33 | -0.23 | -1.27 | -3.49 | -6.97 | -8.08 | -5.00 | -5.84 |
Cell Periphery | 0.15 | -5.38 | -4.97 | -6.50 | 1.96 | -3.95 | -5.96 | -5.74 | -3.57 | -2.49 | -1.62 | -5.78 | -9.08 | -8.35 | -4.65 |
Cytoplasm | 18.92 | 67.99* | 39.01 | 4.76 | -24.08 | 1.77 | 51.23 | 19.40 | 17.48 | -2.85 | 12.46 | 77.16 | 34.51 | 17.51 | -24.28 |
Cytoplasmic Foci | 6.12 | 24.99 | 22.46 | 7.36 | -8.37 | -2.61 | 20.35 | 19.07 | 18.40 | -3.81 | 3.71 | 31.44 | 25.32 | 16.87 | -9.78 |
Eisosomes | 0.58 | -3.06 | 0.99 | 0.06 | 3.59 | -4.14 | -5.26 | -6.57 | -5.54 | -4.82 | -0.13 | -3.66 | -5.93 | -5.52 | -1.26 |
Endoplasmic Reticulum | -6.45 | -12.46 | -18.92 | -5.31 | 8.95 | -2.37 | -15.57 | -7.90 | -7.73 | -2.76 | -6.72 | -19.52 | -18.48 | -14.12 | 10.13 |
Endosome | -1.91 | 1.23 | -3.79 | -1.90 | -4.56 | -4.77 | 2.64 | -2.23 | 0.54 | -3.28 | -4.17 | 3.46 | -4.67 | -3.18 | -5.52 |
Golgi | -1.28 | -4.59 | -3.37 | -2.30 | -3.09 | -2.55 | 0.96 | -1.03 | 2.51 | -1.70 | -2.70 | -1.74 | -3.37 | -0.91 | -3.33 |
Lipid Particles | -0.40 | 3.18 | 1.84 | 1.97 | -0.67 | -1.88 | 6.80 | 1.64 | 3.43 | -3.65 | -1.37 | 8.33 | 1.26 | 2.13 | -2.90 |
Mitochondria | 0.58 | 0.66 | -1.57 | -3.27 | -3.88 | -2.99 | -0.05 | 0.41 | 3.65 | 0.98 | -2.14 | 0.49 | -2.74 | -0.91 | -3.72 |
Mitotic Spindle | -2.00 | -2.53 | -11.59 | -7.42 | -11.42 | -3.45 | -2.35 | -1.89 | -1.14 | -1.27 | -4.14 | -2.56 | -11.56 | -9.54 | -10.02 |
None | 3.05 | 8.55 | -1.13 | -3.78 | -8.04 | -0.92 | 3.37 | 1.43 | 1.78 | -0.19 | 0.57 | 5.58 | -2.01 | -2.31 | -5.32 |
Nuclear Periphery | -7.79 | -6.34 | -12.66 | -6.53 | -8.26 | 0.51 | -2.45 | -3.95 | -4.81 | -2.56 | -1.66 | -4.47 | -8.17 | -8.08 | -4.48 |
Nuclear Periphery Foci | -2.60 | -3.91 | -4.85 | 0.58 | 1.06 | 0.16 | -0.25 | -2.46 | -2.52 | -2.40 | -2.19 | -2.91 | -4.23 | -2.63 | -2.29 |
Nucleolus | -1.97 | -4.71 | -7.13 | -0.54 | 1.45 | -3.18 | -1.85 | -0.13 | 2.29 | 1.06 | -4.37 | -4.69 | -4.57 | 0.83 | -0.06 |
Nucleus | -14.81 | -47.51 | -27.66 | -1.09 | 19.15 | 2.39 | -37.20 | -6.57 | -7.34 | 4.68 | -8.45 | -57.29 | -16.90 | -7.67 | 25.66 |
Peroxisomes | -2.44 | 0.14 | -0.39 | 2.17 | -0.46 | -2.59 | 3.88 | 3.61 | 4.68 | -1.72 | -3.46 | 4.40 | 0.58 | 3.55 | -2.20 |
Vacuole | -4.52 | -3.65 | -12.11 | -2.40 | -3.93 | -6.20 | -4.14 | -4.67 | -4.14 | -3.91 | -6.85 | -4.73 | -14.21 | -9.78 | -7.97 |
Vacuole Periphery | -2.25 | -3.51 | -4.10 | -3.73 | -3.69 | -4.21 | 3.14 | -0.39 | 3.88 | -2.66 | -4.60 | 0.95 | -4.06 | -3.14 | -4.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|