Standard name
Human Ortholog
Description Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.31 0.29 0.32 0.32 0.32 0.24 0.24 0.31 0.26 0.26 0.26 0.35 0.35 0.36 0.37 0.35 0.31 0.18 0.3 0.24 0.31 0.24 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0.11 0.08 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.47 0.48 0.64 0.38 0.55 0.62 0.65 0.68 0.7 0.74 0.76 0.35 0.07 0.16 0 0 0 0 0 0.08 0 0.05 0
Nucleus 0.34 0.09 0.12 0.13 0.19 0.13 0.22 0.19 0.19 0.16 0.14 0.12 0.05 0.18 0.13 0.11 0.12 0.14 0.38 0.31 0.26 0.29 0.24 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05
Vac/Vac Membrane 0.53 0.31 0.21 0.13 0.23 0.19 0.16 0.17 0.11 0.14 0.11 0.16 0.45 0.47 0.45 0.55 0.58 0.61 0.24 0.19 0.29 0.18 0.26 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0
Bud 0 0 4 8 0 4 5 11 4 13 6 6 0 0 2 0 0 0 2 3 1 1 7 5
Bud Neck 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2
Bud Site 0 0 0 1 1 0 3 11 1 6 6 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 12 14 60 74 86 102 108 131 83 87 69 96 7 48 60 120 92 100 15 29 74 9 26 18
Endoplasmic Reticulum 0 1 2 0 1 0 0 1 1 0 0 1 0 0 1 9 14 11 0 0 2 1 0 2
Endosome 5 0 0 1 8 2 5 3 0 0 1 0 0 7 4 26 30 26 3 1 7 1 5 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0
Mitochondria 0 21 99 145 103 174 274 352 181 234 198 284 7 10 26 4 1 7 1 3 25 0 5 2
Nucleus 16 4 25 29 52 42 98 102 50 53 37 44 1 25 21 37 33 44 33 30 82 8 26 18
Nuclear Periphery 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 0 3 4 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 0 2 2 1 2 1 0 0 0 0 0 1 2 4 4 11 1 4 4
Vac/Vac Membrane 25 14 43 30 61 61 72 95 30 48 29 59 9 66 75 179 155 195 21 18 89 5 28 28
Unique Cell Count 47 45 208 228 269 314 441 543 266 333 266 375 20 139 166 323 265 322 89 99 312 30 109 92
Labelled Cell Count 58 54 235 290 316 385 572 714 353 443 347 499 24 156 189 376 327 387 89 99 312 30 109 92


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 3.8 3.7 3.5 4.2 3.9 3.7 3.8 3.5 3.6 3.6 3.3 4.6 5.4 4.8 7.4 7.7 8.0 – 4.8 6.4
Std Deviation (1e-4) 1.0 0.6 0.6 0.8 1.3 1.0 1.0 1.0 1.1 1.4 1.2 1.0 0.8 1.6 1.2 1.1 1.4 1.6 – 0.8 1.6
Intensity Change (Log2) – – – -0.09 0.17 0.05 0.0 0.02 -0.09 -0.05 -0.06 -0.18 0.32 0.54 0.36 0.98 1.04 1.1 – 0.38 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.9 8.3 9.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0573 2.2562 2.2106 1.8922 1.9444 1.9046 1.5016 2.6117 2.2397 1.9465 2.1548 1.9797 3.5829 3.5078 2.884 3.5849 2.8282 3.2776
Actin 0.0527 0.0004 0.0122 0.0252 0.0389 0.0003 0.0306 0.0005 0.0134 0.0006 0.0233 0.0006 0.0391 0.0003 0.0025 0.0002 0.0001 0.0094
Bud 0.0015 0.0003 0.0004 0.0005 0.0019 0.0002 0.001 0.0003 0.0009 0.0001 0.0006 0.0002 0.0062 0.0001 0.0002 0.0001 0 0.0005
Bud Neck 0.0055 0.0011 0.0096 0.0047 0.002 0.0044 0.0058 0.0008 0.0014 0.0011 0.0021 0.0041 0.0121 0.0003 0.0007 0.0006 0.0006 0.0049
Bud Periphery 0.0029 0.0001 0.0003 0.0007 0.0032 0.0005 0.0013 0.0001 0.0009 0 0.0007 0.0001 0.0023 0 0.0002 0.0001 0 0.0005
Bud Site 0.0102 0.0021 0.0037 0.0022 0.0063 0.0002 0.0136 0.0024 0.0065 0.0002 0.0046 0.0003 0.024 0.0006 0.0011 0.0001 0.0001 0.0009
Cell Periphery 0.0007 0.0001 0.0002 0.0002 0.0005 0.0007 0.0005 0.0001 0.0001 0 0.0002 0 0.0006 0 0.0001 0 0 0.0001
Cytoplasm 0.1579 0.2869 0.201 0.1447 0.2892 0.1538 0.1839 0.2612 0.178 0.1505 0.1489 0.1476 0.1605 0.3454 0.2545 0.0909 0.3131 0.2132
Cytoplasmic Foci 0.0288 0.0048 0.0127 0.0174 0.0066 0.0027 0.0198 0.0049 0.0166 0.0022 0.0164 0.0032 0.0231 0.0012 0.007 0.0004 0.0007 0.014
Eisosomes 0.0011 0 0.0003 0.0003 0.0004 0.0001 0.0003 0.0001 0.0001 0 0.0008 0 0.0002 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0178 0.0086 0.0108 0.0101 0.0128 0.0061 0.0087 0.0032 0.0033 0.0052 0.0032 0.0022 0.0062 0.0047 0.0078 0.0009 0.0028 0.0049
Endosome 0.0428 0.0054 0.0191 0.018 0.027 0.0037 0.0305 0.003 0.0124 0.003 0.0203 0.0061 0.0201 0.0024 0.0115 0.0005 0.0012 0.0119
Golgi 0.0121 0.0002 0.0031 0.0146 0.0107 0.0002 0.0073 0.0001 0.0044 0.0001 0.006 0.0016 0.0093 0 0.0056 0 0 0.0019
Lipid Particles 0.015 0.0003 0.0094 0.0091 0.0054 0.0003 0.0109 0.0002 0.0033 0.0002 0.0117 0.0011 0.0081 0 0.001 0 0 0.0041
Mitochondria 0.0167 0.0159 0.0025 0.0042 0.0434 0.0081 0.0163 0.001 0.0057 0.0011 0.0056 0.0006 0.0037 0.0004 0.002 0.0003 0.0004 0.0027
None 0.0614 0.0348 0.0334 0.0127 0.0642 0.0089 0.1114 0.1086 0.1097 0.1372 0.092 0.0536 0.0473 0.02 0.0912 0.0873 0.0563 0.0643
Nuclear Periphery 0.0589 0.0234 0.0355 0.0218 0.0333 0.0198 0.0272 0.0188 0.0153 0.042 0.0322 0.0107 0.0433 0.0281 0.0354 0.0073 0.0112 0.0209
Nucleolus 0.0066 0.0031 0.0041 0.0022 0.0017 0.0042 0.0035 0.0023 0.0025 0.0019 0.0091 0.0038 0.003 0.0011 0.0021 0.001 0.0016 0.0029
Nucleus 0.454 0.577 0.6021 0.6701 0.4295 0.7613 0.4738 0.5748 0.5894 0.6163 0.5481 0.7353 0.5315 0.5861 0.5545 0.8053 0.6041 0.5863
Peroxisomes 0.0137 0.0003 0.008 0.0203 0.0012 0 0.0097 0.0001 0.0051 0.0001 0.0259 0.0003 0.0071 0 0.0005 0 0 0.0035
Punctate Nuclear 0.0196 0.0276 0.0242 0.0177 0.0118 0.0202 0.0383 0.0145 0.0278 0.0362 0.0453 0.0262 0.047 0.0068 0.0197 0.0044 0.0064 0.0494
Vacuole 0.0145 0.007 0.0061 0.0026 0.0078 0.0035 0.0044 0.0026 0.0024 0.001 0.0021 0.0021 0.0035 0.0019 0.0017 0.0004 0.0009 0.0029
Vacuole Periphery 0.0055 0.0007 0.0012 0.0008 0.0023 0.0006 0.0012 0.0004 0.0007 0.0007 0.0008 0.0003 0.0017 0.0005 0.0007 0.0001 0.0002 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8623 5.1427 6.1058 11.5257 7.4589 11.1161 10.2263 14.1551 16.8539 12.4176
Translational Efficiency 0.7227 0.8178 0.8602 0.9001 0.793 0.9213 0.7072 0.564 0.6773 0.5858

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
Localization
Cell Percentages nucleus (20%), cytoplasm (13%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vid30

Vid30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vid30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available