Standard name
Human Ortholog
Description Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0.05 0.07 0.06 0 0 0 0.1 0.14 0.14 0.08 0.07 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.16 0.09 0.12 0.1 0.56 0.23 0.37 0.34 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.95 0.92 0.94 0.93 0.87 0.89 0.87 0.82 0.72 0.83 0.8 0.82 0.88 0.85 0.83 0.89 0.85 0.84 0.85 0.86 0.85 0.69 0.6 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.06 0.13 0.19 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0
Bud 0 0 0 0 5 0 0 6 9 9 6 0 0 0 2 0 0 1 4 6 3 3 0 3
Bud Neck 0 0 1 0 1 0 0 0 2 1 1 0 1 0 0 0 0 0 0 0 0 0 1 3
Bud Site 1 0 0 2 1 6 3 4 22 20 23 25 0 0 0 0 2 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 25 19 20 27 25 34 56 41 19 16 12 51 71 71 28 27 28 3 4 1 6 6 5
Endoplasmic Reticulum 1 2 0 0 0 0 0 1 0 0 0 0 0 0 1 5 8 9 0 0 1 1 2 0
Endosome 1 0 0 0 0 5 4 6 0 2 1 0 3 0 1 0 3 1 1 5 4 7 6 9
Golgi 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 7 0 2 1 0
Mitochondria 12 28 1 38 71 62 86 73 385 161 206 199 0 1 1 18 8 3 0 6 4 7 11 12
Nucleus 271 588 430 427 380 587 630 616 495 591 444 489 446 442 429 319 311 224 256 575 402 285 215 247
Nuclear Periphery 0 5 0 0 0 6 10 7 9 17 6 4 2 5 1 12 10 8 0 0 1 3 4 13
Nucleolus 7 6 1 2 4 2 7 19 31 25 28 13 1 4 5 8 6 5 13 18 16 29 29 52
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
SpindlePole 0 1 4 2 4 3 3 10 6 5 5 2 1 3 6 2 3 3 3 2 4 5 6 8
Vac/Vac Membrane 6 16 5 7 8 18 16 29 12 16 9 12 5 4 7 7 18 11 12 34 28 55 66 93
Unique Cell Count 286 642 457 460 436 658 728 748 688 709 555 594 504 519 517 358 365 266 300 671 476 413 358 458
Labelled Cell Count 304 671 462 499 501 715 793 827 1012 866 745 756 511 530 525 399 396 294 300 671 476 413 358 458


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.7 6.7 5.8 5.2 5.4 5.3 5.6 4.2 5.0 4.6 4.8 6.7 6.5 6.2 6.7 8.1 8.2 6.9 7.4 7.2
Std Deviation (1e-4) 1.2 1.0 1.3 1.2 1.4 1.3 1.2 1.4 1.1 0.9 1.0 0.8 1.5 1.3 1.3 1.4 2.0 2.3 1.5 1.5 1.5
Intensity Change (Log2) – – – -0.21 -0.35 -0.3 -0.33 -0.25 -0.66 -0.43 -0.54 -0.49 0.0 -0.04 -0.1 -0.0 0.28 0.29 0.06 0.14 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 1.4 -0.3 0.4 2.3 1.3 -1.4 -1.1 -2.0 3.6 5.1 5.1 2.2 2.0 3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.0 8.8 6.5 7.4 6.7 0 10.8 0 0 0 0 0 4.5 0 0
Nucleus -0.8 -3.6 -2.8 -4.1 -5.8 -9.3 -5.4 -6.5 -5.7 -3.1 -4.5 -5.4 -2.6 -4.2 -4.4
Nuclear Periphery 0 0 0 0 0 0 3.3 0 0 0 0 0 3.9 0 0
Nucleolus 0 0 0 0 3.1 0 3.7 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.7 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 0.9 1.9 1.4 2.8 0.9 1.5 0.7 1.2 0 0 0.4 1.0 3.3 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6596 4.2869 3.764 3.5856 3.1801 3.7068 7.5824 6.9073 7.486 7.6629 7.044 7.3078 4.5848 4.4793 4.0759 4.6715 3.7827 4.4499
Actin 0.0246 0 0.0004 0.0026 0.0433 0.0004 0.0165 0.0004 0.0015 0.0001 0.0002 0.0035 0.0003 0 0 0 0 0
Bud 0.0003 0.0001 0 0.0004 0.0005 0.0001 0.0002 0.0008 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0
Bud Neck 0.0058 0.0002 0.0006 0.0012 0.0009 0.0014 0.0006 0.0009 0.0058 0.0007 0.0011 0.0012 0.0004 0.0001 0.0001 0.0003 0.0002 0.0009
Bud Periphery 0.0007 0.0001 0.0001 0.0009 0.0007 0.0001 0.0004 0.0014 0.0002 0.0005 0.0001 0.0005 0.0001 0 0 0.0001 0.0001 0.0001
Bud Site 0.0049 0.0002 0.0002 0.0049 0.0019 0.0002 0.0008 0.0035 0.0035 0.0004 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Cell Periphery 0.0004 0 0.0001 0.0004 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0
Cytoplasm 0.0016 0.0001 0.0003 0.0026 0.0004 0.0001 0.0063 0.0046 0.0003 0.0001 0.0007 0.0003 0.0008 0.0001 0 0.0001 0 0
Cytoplasmic Foci 0.0157 0 0.0023 0.0309 0.0026 0.0014 0.0068 0.0003 0.0068 0 0.015 0.0001 0.0001 0 0 0 0 0
Eisosomes 0.0004 0 0.0002 0.0001 0.0002 0 0.0002 0 0 0 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0006 0 0.0007 0.0049 0.0044 0 0.0019 0.0001 0.0001 0.0001 0.0002 0.0013 0.0006 0 0 0 0 0
Endosome 0.0088 0 0.0026 0.0388 0.0209 0.0008 0.0165 0.0007 0.0032 0.0001 0.0009 0.0005 0.0008 0 0 0 0 0
Golgi 0.0057 0 0.0006 0.0076 0.0124 0.0006 0.0073 0.0002 0.0021 0 0.0002 0.0001 0.0001 0 0 0 0 0
Lipid Particles 0.014 0 0.0068 0.0739 0.0023 0.0125 0.0209 0.0002 0.0074 0 0.0014 0.0001 0.0002 0 0 0 0 0
Mitochondria 0.0103 0.0003 0.0017 0.0032 0.005 0.0087 0.0091 0.0054 0.0039 0.001 0.0012 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001
None 0.0039 0 0.0024 0.0016 0.0005 0.0001 0.0027 0.0002 0.0004 0.0001 0.0325 0.0002 0.0002 0 0 0 0 0
Nuclear Periphery 0.0019 0.001 0.003 0.0398 0.0108 0.0004 0.0105 0.0104 0.001 0.0491 0.0054 0.0033 0.0146 0.0007 0.0005 0.0025 0.0004 0.0003
Nucleolus 0.0527 0.0614 0.0779 0.1272 0.0795 0.1039 0.0389 0.1011 0.0351 0.0593 0.1086 0.0871 0.0254 0.0469 0.0453 0.1041 0.1139 0.1011
Nucleus 0.8127 0.9358 0.8951 0.625 0.8072 0.858 0.8331 0.8611 0.9173 0.8868 0.7988 0.895 0.9515 0.9502 0.9531 0.8899 0.8844 0.896
Peroxisomes 0.0176 0 0.0018 0.0132 0.0017 0.01 0.003 0.0005 0.0083 0 0.0005 0 0 0 0 0 0 0
Punctate Nuclear 0.015 0.0005 0.0015 0.0127 0.0015 0.0008 0.0202 0.001 0.0019 0.0005 0.0319 0.0057 0.0037 0.0016 0.0005 0.002 0.0006 0.001
Vacuole 0.0018 0.0002 0.001 0.006 0.0009 0.0003 0.0021 0.0056 0.0008 0.0006 0.0003 0.0005 0.0003 0.0001 0.0001 0.0005 0.0001 0.0002
Vacuole Periphery 0.0006 0.0001 0.0007 0.0021 0.0021 0.0002 0.0018 0.0012 0.0002 0.0004 0.0001 0.0002 0.0003 0 0 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.2191 45.3506 46.2717 46.1473 47.6871 27.8867 49.5796 42.9408 42.2044 47.9414
Translational Efficiency 1.4763 1.463 1.4013 1.548 1.4002 2.4211 1.3207 1.5587 1.4933 1.52

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2311 1504 2622 1504 2031 2285 2342 1571 4342 3789 4964 3075

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.83 822.63 927.02 999.14 726.00 903.58 928.97 1056.02 740.81 871.45 927.94 1028.20
Standard Deviation 105.98 127.39 145.95 168.03 97.60 117.39 147.04 172.24 103.08 127.75 146.47 172.55
Intensity Change Log 2 — 0.126004 0.298361 0.406448 — 0.315683 0.355662 0.540596 — 0.222177 0.326757 0.473819

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000137 0.000428 0.000498 0.000823 0.000072 0.000150 0.000564 0.000923 0.000106 0.000260 0.000529 0.000874
Bud Neck 0.005334 0.010539 0.030786 0.028305 0.002654 0.007763 0.026963 0.040029 0.004080 0.008865 0.028982 0.034295
Bud Site 0.001501 0.002031 0.001968 0.003101 0.000313 0.001510 0.001498 0.004589 0.000945 0.001717 0.001746 0.003861
Cell Periphery 0.000664 0.000229 0.000104 0.000345 0.000168 0.000267 0.000136 0.000134 0.000432 0.000252 0.000119 0.000237
Cytoplasm 0.018453 0.005837 0.001646 0.004140 0.007011 0.008622 0.002249 0.003444 0.013101 0.007517 0.001931 0.003785
Cytoplasmic Foci 0.001453 0.001191 0.000065 0.001564 0.001236 0.001552 0.000135 0.001045 0.001351 0.001409 0.000098 0.001299
Eisosomes 0.000021 0.000025 0.000034 0.000031 0.000015 0.000021 0.000037 0.000036 0.000018 0.000022 0.000036 0.000033
Endoplasmic Reticulum 0.006464 0.008582 0.004593 0.004350 0.002769 0.006933 0.005527 0.004944 0.004735 0.007587 0.005034 0.004653
Endosome 0.000655 0.001013 0.000306 0.001768 0.000410 0.001161 0.000494 0.001637 0.000541 0.001102 0.000395 0.001701
Golgi 0.000312 0.000499 0.000058 0.000889 0.000281 0.000261 0.000148 0.002153 0.000297 0.000356 0.000100 0.001535
Lipid Particles 0.000312 0.000296 0.000179 0.000386 0.000467 0.000713 0.000185 0.000753 0.000385 0.000547 0.000182 0.000574
Mitochondria 0.001512 0.001862 0.001611 0.003653 0.001403 0.002786 0.001615 0.004138 0.001461 0.002419 0.001613 0.003900
Mitotic Spindle 0.000373 0.001120 0.001639 0.011924 0.000885 0.001830 0.001264 0.007121 0.000612 0.001549 0.001462 0.009470
None 0.006423 0.004435 0.001945 0.004324 0.008679 0.006696 0.003928 0.001571 0.007478 0.005798 0.002881 0.002917
Nuclear Periphery 0.001029 0.000833 0.001359 0.003137 0.000895 0.001710 0.001074 0.001966 0.000966 0.001362 0.001225 0.002539
Nuclear Periphery Foci 0.000246 0.000296 0.000270 0.001973 0.000301 0.000334 0.000625 0.001524 0.000272 0.000319 0.000437 0.001744
Nucleolus 0.010987 0.019855 0.018717 0.011809 0.016858 0.017385 0.017942 0.014130 0.013733 0.018366 0.018351 0.012995
Nucleus 0.941128* 0.938568* 0.931293* 0.910796* 0.954633* 0.935062* 0.932639* 0.902396* 0.947445* 0.936454* 0.931928* 0.906504*
Peroxisomes 0.000480 0.000478 0.000173 0.000403 0.000245 0.000594 0.000191 0.000197 0.000370 0.000548 0.000181 0.000297
Vacuole 0.002188 0.001511 0.001769 0.004482 0.000563 0.003874 0.001735 0.004964 0.001428 0.002936 0.001753 0.004728
Vacuole Periphery 0.000329 0.000373 0.000986 0.001799 0.000142 0.000776 0.001053 0.002306 0.000242 0.000616 0.001017 0.002058

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.86 -14.32 -11.99 -7.35 -6.05 -3.64 -26.63* -5.01 -4.55 -2.15 -8.64 -26.21 -8.86 -7.19 -4.23
Bud Neck -5.57 -18.47 -18.33 -13.30 -0.56 -8.76 -20.57 -22.58 -19.59 -7.62 -9.17 -27.23 -28.80 -23.93 -6.08
Bud Site -1.09 -1.38 -3.63 -2.17 -2.48 -5.09 -9.05 -5.61 -4.07 -4.13 -2.83 -3.65 -6.51 -4.79 -4.74
Cell Periphery 3.01 4.44 2.22 -0.08 -0.97 -2.54 1.65 1.90 4.10 0.54 2.36 4.64 2.59 0.94 -0.90
Cytoplasm 7.29 11.03 8.11 0.72 -3.49 -1.32 5.50 3.65 4.84 -2.01 5.16 12.27 8.99 4.03 -4.02
Cytoplasmic Foci 0.51 5.68 1.17 0.42 -2.18 -0.46 3.08 1.19 1.72 -1.91 -0.05 5.83 1.71 1.52 -2.91
Eisosomes -2.15 -6.55 -7.02 -4.69 -0.01 -3.16 -14.53 -8.85 -5.54 0.86 -3.18 -13.20 -11.00 -7.40 0.58
Endoplasmic Reticulum -2.55 2.40 2.08 7.42 -0.87 -8.69 -5.01 -8.32 3.58 0.70 -5.66 -0.73 -0.71 7.15 0.16
Endosome -0.80 2.47 -1.87 -0.50 -3.21 -4.33 -0.33 -2.84 -0.41 -2.58 -2.69 1.74 -3.26 -0.64 -4.10
Golgi -0.57 1.67 -1.18 -0.30 -2.91 0.06 0.59 -1.93 -2.07 -2.29 -0.31 1.44 -2.22 -2.08 -3.00
Lipid Particles 0.20 1.02 -0.09 -0.21 -0.89 -1.13 2.41 -0.15 0.32 -0.85 -1.33 2.43 -0.19 0.41 -1.08
Mitochondria -0.55 -0.36 -2.17 -1.89 -2.42 -1.84 -0.59 -3.49 -1.86 -3.89 -1.87 -0.66 -4.04 -2.43 -4.51
Mitotic Spindle -1.38 -2.58 -5.84 -5.42 -4.94 -1.23 -0.68 -4.56 -3.78 -4.22 -1.88 -2.36 -7.44 -6.50 -6.45
None 2.38 6.04 2.68 0.50 -3.38 1.49 4.12 6.42 5.81 3.19 2.06 6.78 6.75 4.78 -0.23
Nuclear Periphery 1.46 -1.06 -2.88 -3.25 -2.00 -4.51 -1.19 -4.15 -1.45 -2.76 -3.20 -1.51 -4.53 -3.25 -3.08
Nuclear Periphery Foci -0.65 -0.25 -2.25 -2.14 -2.19 -0.19 -0.80 -1.86 -1.77 -1.15 -0.49 -0.84 -2.92 -2.75 -2.39
Nucleolus -5.84 -6.49 -2.32 2.99 3.18 -0.28 -0.91 0.08 0.32 0.88 -4.31 -5.19 -1.60 2.14 2.81
Nucleus 0.65 3.56 7.43 6.79 5.06 4.85 6.85 12.03 8.08 7.44 3.76 7.12 13.61 10.39 8.84
Peroxisomes 0.03 1.40 0.63 1.20 -1.88 -2.10 1.41 0.98 2.76 -0.82 -1.17 1.75 0.92 2.84 -2.03
Vacuole 2.04 0.86 -4.64 -5.46 -4.91 -5.54 -3.67 -6.08 -2.24 -4.24 -3.88 -1.70 -7.48 -4.75 -6.47
Vacuole Periphery -0.45 -1.62 -2.38 -2.30 -0.52 -5.64 -2.09 -3.68 -2.20 -1.34 -4.44 -2.61 -4.32 -3.00 -1.37
Standard name
Human Ortholog
Description Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Tan1

Tan1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tan1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available