Standard name
Human Ortholog
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.08 0.05 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0.06 0.05 0 0 0 0.12 0.16 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.28 0 0 0 0.1 0.27 0.29 0.29 0.23 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.87 0.94 0.98 0.95 0.89 0.87 0.79 0.78 0.79 0.88 0.83 0.77 0.88 0.84 0.84 0.87 0.82 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.1 0.12 0.26 0.23 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0.09 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 0 0 1 0 0 0 1 1 0 0 0 0 2 0 1 2 0 3
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 0 0 2 1 5 2 8 5 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 4 4 1 1 11 5 5 1 3 42 64 88 1 4 0 1 5 5
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1
Endosome 0 1 0 0 1 0 0 0 0 0 0 2 4 1 1 0 0 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0
Mitochondria 20 47 1 0 2 18 29 34 30 25 2 0 1 2 3 0 2 1 7
Nucleus 97 144 72 83 122 157 94 92 81 84 309 341 336 105 148 72 185 279 267
Nuclear Periphery 0 3 0 0 1 3 2 1 0 2 0 0 0 0 0 0 0 1 1
Nucleolus 2 3 0 1 3 5 11 14 27 25 0 0 4 0 0 1 4 9 20
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 2 0 0 1 0 1 3 4 0 0 1 1 3 5
Vac/Vac Membrane 0 3 1 0 1 2 0 0 1 0 1 3 7 4 13 3 11 31 55
Unique Cell Count 105 166 77 85 128 177 108 117 104 107 353 410 436 120 176 86 212 342 387
Labelled Cell Count 120 208 79 86 134 199 146 149 150 144 357 416 448 120 176 86 212 342 387


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.1 6.0 6.3 5.9 5.5 5.3 4.9 5.1 5.3 6.5 6.9 6.9 6.9 7.2 7.4
Std Deviation (1e-4) 0.7 5.4 1.1 0.8 1.1 1.4 1.2 1.0 1.4 1.5 1.3 1.5 2.2 1.4 1.7 1.8
Intensity Change (Log2) 0.08 -0.03 -0.13 -0.17 -0.3 -0.24 -0.19 0.12 0.2 0.2 0.21 0.26 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 1.7 2.4 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.5 4.6 0 4.9 4.2 0 0 0
Nucleus 1.3 0.6 -1.2 -1.4 -2.8 -2.9 -2.8 -1.5 -2.3 -3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 4.8 4.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7396 1.5246 1.3781 1.3975 1.1355 1.5299 5.7084 5.1886 5.3343 5.0059 5.1836 5.3762 3.0844 2.4271 2.6348 1.9115 2.1852 2.4511
Actin 0.0195 0 0.0023 0 0 0.0002 0.0068 0 0.0148 0.0044 0.0035 0.0001 0.0235 0 0.0083 0 0.022 0.0001
Bud 0.0001 0 0 0 0 0 0.0005 0 0.0008 0.001 0.0004 0 0.0002 0 0.0002 0 0.0004 0.0001
Bud Neck 0.0001 0.0002 0.0001 0 0.0002 0.001 0.0031 0.0001 0.0031 0.0002 0.004 0.0008 0.0022 0.0001 0.0007 0.0001 0.0025 0.0011
Bud Periphery 0.0002 0 0 0 0 0.0001 0.001 0 0.0015 0.0033 0.0011 0.0001 0.0002 0 0.0003 0 0.0005 0.0001
Bud Site 0.0002 0.0001 0.0001 0 0 0.0002 0.0043 0.0001 0.0079 0.0031 0.0009 0.0001 0.0013 0.0001 0.0007 0 0.0058 0.0002
Cell Periphery 0 0 0 0 0 0 0.0004 0 0.0005 0.0002 0.0003 0 0.0001 0 0.0001 0 0.0002 0
Cytoplasm 0.0019 0.0005 0.0002 0 0.0002 0.0001 0.0055 0.0023 0.0033 0.0012 0.0024 0.0002 0.0189 0.0006 0.005 0.0001 0.0027 0.0018
Cytoplasmic Foci 0.0015 0.0013 0.0006 0 0 0.0009 0.0063 0 0.0039 0.0005 0.0067 0 0.0228 0 0.0047 0 0.0115 0.0001
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0009 0 0 0 0 0.0001 0.0051 0.0001 0.0028 0.0036 0.0017 0.0001 0.0037 0.0001 0.0016 0 0.0015 0.0002
Endosome 0.0041 0.0003 0.0002 0 0 0.0011 0.0268 0.0002 0.0136 0.0046 0.0173 0.0004 0.02 0.0001 0.0112 0 0.0095 0.0009
Golgi 0.0015 0.0001 0.0003 0 0 0.0002 0.0103 0 0.0057 0.0016 0.0072 0 0.004 0 0.0039 0 0.0032 0
Lipid Particles 0.0036 0.0001 0.0022 0 0 0.0022 0.0163 0 0.0064 0.001 0.0205 0.0002 0.0067 0 0.0049 0 0.0091 0.0001
Mitochondria 0.0011 0.0001 0.0003 0 0.0005 0.0003 0.0203 0.0002 0.017 0.0166 0.0044 0.0005 0.0011 0.0001 0.0019 0.0001 0.0016 0.0002
None 0.0014 0.0001 0.0001 0 0.0001 0.0002 0.0027 0.0022 0.0006 0.0003 0.0006 0.0001 0.0149 0.0001 0.0086 0 0.0017 0.0013
Nuclear Periphery 0.0064 0.0011 0.0017 0.0003 0.0021 0.0012 0.0479 0.0034 0.0058 0.0024 0.0372 0.0033 0.0112 0.0045 0.0039 0.0006 0.0168 0.0026
Nucleolus 0.0065 0.0118 0.006 0.0081 0.0373 0.0248 0.0207 0.0081 0.0062 0.0034 0.0158 0.0188 0.009 0.0101 0.0081 0.0049 0.0181 0.017
Nucleus 0.9462 0.9787 0.9815 0.9908 0.9584 0.9624 0.7988 0.9794 0.8864 0.9499 0.8426 0.9734 0.828 0.9826 0.9225 0.9938 0.8696 0.9688
Peroxisomes 0.0013 0.0001 0.003 0 0 0.0005 0.0053 0 0.0038 0.0007 0.0014 0 0.011 0 0.0031 0 0.0052 0
Punctate Nuclear 0.0016 0.0053 0.0015 0.0007 0.0009 0.0043 0.0092 0.0024 0.0108 0.0005 0.0257 0.0015 0.0196 0.0014 0.0082 0.0003 0.0159 0.0047
Vacuole 0.0009 0.0001 0 0 0.0001 0.0002 0.0058 0.0011 0.0034 0.0012 0.0036 0.0002 0.0009 0.0001 0.0014 0 0.0015 0.0007
Vacuole Periphery 0.0007 0 0 0 0.0001 0.0001 0.0025 0.0004 0.0014 0.0005 0.0029 0.0002 0.0004 0.0001 0.0005 0 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2963 9.8885 12.8409 9.5032 8.0674 9.6736 13.4351 10.544 10.7876 11.1738
Translational Efficiency 1.9042 2.2117 1.2086 1.5896 2.1842 2.0509 1.2526 2.0353 1.7213 1.5821

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hap2

Hap2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hap2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available