Standard name
Human Ortholog
Description Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.1 0.09 0.09 0.13 0.08 0.13 0.08 0.11 0.08 0.08 0.05 0.13 0.15 0.26 0.2 0.13 0.16 0.05 0.06 0 0.15 0.14 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.17 0 0.07 0 0.11 0.13 0.15 0.59 0.33 0.4 0.52 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Nucleus 0.8 0.74 0.86 0.82 0.78 0.82 0.73 0.75 0.61 0.71 0.69 0.63 0.75 0.82 0.68 0.53 0.6 0.7 0.77 0.73 0.79 0.62 0.56 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.15 0.07 0.05 0.08 0.07 0.06 0.08 0.05 0.07 0.09 0.1 0.16 0.08 0.09 0.3 0.35 0.22 0.1 0.14 0.11 0.15 0.17 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 0 0 0 1 1 3 2 4 5 0 0 0 0 0 0 10 7 2 0 3 7
Bud Neck 0 0 0 0 0 0 0 4 3 1 2 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 2 2 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 29 38 17 17 30 21 44 35 41 29 20 15 19 28 60 24 20 21 14 26 6 14 32 26
Endoplasmic Reticulum 1 1 1 0 1 0 0 1 0 0 0 0 0 3 1 8 7 5 0 0 0 0 1 1
Endosome 0 1 2 2 6 1 2 4 0 0 0 1 3 0 1 2 3 0 2 2 1 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0
Mitochondria 2 66 0 12 2 30 44 64 226 119 99 143 3 0 2 8 7 1 0 5 1 0 1 4
Nucleus 242 287 158 150 176 220 241 314 232 255 169 173 107 159 156 63 90 92 251 328 164 58 132 130
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Nucleolus 0 1 0 0 2 0 2 1 1 0 0 4 0 0 0 0 0 0 0 0 0 0 3 4
Peroxisomes 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 1 0 1 4 1 6 0 3 1 0 0 1 0 0 8 9 3 2 5 9
Vac/Vac Membrane 59 59 12 10 17 18 21 32 21 25 21 27 23 15 21 35 53 29 31 62 23 14 40 58
Unique Cell Count 303 387 184 182 225 268 330 417 382 361 246 276 142 193 229 118 151 132 326 453 208 94 236 256
Labelled Cell Count 333 453 191 193 235 290 357 460 530 440 317 372 156 205 241 141 180 148 326 453 208 94 236 256


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.4 6.2 5.7 6.2 5.1 4.9 5.1 4.0 4.4 4.4 4.2 5.8 5.9 5.4 5.3 6.4 6.8 6.6 6.5 6.3
Std Deviation (1e-4) 0.8 0.8 1.2 1.2 1.3 1.2 1.3 1.2 0.8 0.7 0.9 1.0 1.1 0.8 1.1 1.4 1.1 1.5 1.7 1.7 1.6
Intensity Change (Log2) – – – -0.13 0.0 -0.28 -0.34 -0.27 -0.64 -0.5 -0.5 -0.55 -0.09 -0.08 -0.19 -0.23 0.04 0.13 0.08 0.07 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.4 6.6 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9015 3.4338 3.4556 2.7346 2.734 2.8927 4.9487 4.2218 4.4209 4.8104 4.4695 4.3635 1.9413 2.2686 2.1829 1.9453 1.7279 2.3195
Actin 0.0102 0.0001 0.0298 0.0435 0.0001 0.0001 0.0251 0.0002 0.0003 0.0139 0.0013 0.0006 0.1173 0.0003 0.011 0.0413 0.0001 0.0124
Bud 0.0003 0.0001 0.0003 0.0005 0.0004 0.0002 0.0011 0.0001 0.0001 0.0003 0.0002 0 0.0012 0.0001 0.0001 0.0006 0 0.0001
Bud Neck 0.0017 0.0004 0.002 0.0002 0.0036 0.0024 0.0018 0.0006 0.0001 0.0004 0.0004 0.001 0.0176 0.0003 0.0004 0.0016 0.0007 0.0014
Bud Periphery 0.0008 0 0.0003 0.001 0.0004 0.0002 0.0061 0.0001 0.0002 0.0003 0.0001 0 0.0021 0.0001 0.0001 0.0016 0 0.0002
Bud Site 0.0024 0.0005 0.0125 0.0012 0.0083 0.0002 0.0014 0.0061 0.0003 0.0137 0.0003 0.0001 0.0077 0.0006 0.0006 0.0018 0.0002 0.0001
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0003 0 0.0017 0.0003 0.0001 0.0004 0 0 0.0008 0 0.0001 0.0003 0 0
Cytoplasm 0.0192 0.0438 0.0163 0.0128 0.0113 0.0212 0.0154 0.1567 0.0434 0.0547 0.1485 0.0177 0.026 0.0401 0.0198 0.0275 0.0158 0.0063
Cytoplasmic Foci 0.0155 0.0003 0.009 0.0008 0.0434 0.0001 0.0179 0.0006 0.0008 0.0044 0.0027 0.0004 0.0155 0.0008 0.0072 0.0036 0.0005 0.002
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0006 0 0.0001 0.0002 0 0 0.0008 0 0.0001 0.0005 0 0
Endoplasmic Reticulum 0.0028 0.0019 0.0026 0.0008 0.0019 0.0004 0.0051 0.0054 0.0034 0.0065 0.0045 0.0006 0.0062 0.002 0.001 0.0013 0.0003 0.0017
Endosome 0.0106 0.0009 0.014 0.0056 0.0317 0.0008 0.0262 0.0017 0.0014 0.0037 0.0067 0.0012 0.015 0.0009 0.0018 0.0045 0.0006 0.0077
Golgi 0.0051 0 0.0066 0.0026 0.004 0 0.0228 0 0.0002 0.0013 0.0004 0.0001 0.0073 0 0.0015 0.0021 0 0.0045
Lipid Particles 0.0126 0 0.004 0.0052 0.024 0 0.0115 0.0001 0.0003 0.0007 0.0001 0.0001 0.0154 0.0002 0.0053 0.0115 0 0.0025
Mitochondria 0.0099 0.0002 0.0039 0.0169 0.0016 0.0009 0.0448 0.0006 0.0019 0.0011 0.0007 0.0002 0.0039 0.0002 0.001 0.0049 0.0002 0.008
None 0.0181 0.0113 0.0101 0.0358 0.0007 0.0003 0.0961 0.0188 0.0728 0.099 0.0961 0.026 0.0325 0.0462 0.0253 0.0608 0.0133 0.0183
Nuclear Periphery 0.0106 0.012 0.0065 0.0059 0.0088 0.0037 0.0392 0.0423 0.0094 0.0067 0.0195 0.0113 0.0147 0.0109 0.0052 0.0078 0.0041 0.0206
Nucleolus 0.0045 0.0033 0.0036 0.0021 0.0129 0.005 0.0058 0.0046 0.0023 0.0031 0.0021 0.0045 0.0073 0.0026 0.0023 0.0087 0.0046 0.0033
Nucleus 0.8237 0.918 0.8662 0.857 0.8196 0.9594 0.6196 0.7502 0.8595 0.7805 0.7053 0.9216 0.6436 0.8862 0.9016 0.8023 0.942 0.9001
Peroxisomes 0.0227 0 0.0068 0.0008 0.0083 0 0.0025 0 0.0001 0.0005 0.0001 0 0.0339 0 0.0101 0.0048 0 0.0048
Punctate Nuclear 0.0212 0.0051 0.0032 0.004 0.0033 0.0017 0.0349 0.0074 0.0019 0.0077 0.0092 0.0141 0.0282 0.0069 0.0043 0.0103 0.0169 0.0046
Vacuole 0.0064 0.0017 0.0017 0.0013 0.0141 0.0027 0.0114 0.0035 0.0011 0.0007 0.0011 0.0003 0.0025 0.0012 0.0012 0.0015 0.0006 0.0005
Vacuole Periphery 0.0011 0.0002 0.0004 0.0016 0.0011 0.0008 0.009 0.0006 0.0004 0.0002 0.0005 0.0001 0.0007 0.0002 0.0001 0.0006 0.0001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.034 14.2477 13.8254 11.6754 9.5374 15.9292 11.9125 12.7146 11.7122 14.4049
Translational Efficiency 1.6033 1.1536 1.0509 1.2467 1.9777 1.2013 1.276 1.4659 1.5437 1.3863

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2080 442 192 1411 1750 1983 337 1389 3830 2425 529 2800

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 772.34 764.41 941.49 921.02 715.42 846.35 999.85 934.56 746.33 831.41 978.67 927.74
Standard Deviation 87.50 136.11 118.82 138.56 81.79 102.88 117.45 128.43 89.55 114.16 121.24 133.81
Intensity Change Log 2 — -0.014889 0.285710 0.253996 — 0.242464 0.482921 0.385497 — 0.114600 0.383911 0.318728

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000047 0.000364 0.001056 0.001357 0.000038 0.000206 0.001430 0.001022 0.000043 0.000234 0.001294 0.001191
Bud Neck 0.001876 0.004319 0.003445 0.009885 0.001285 0.004650 0.011999 0.015059 0.001606 0.004589 0.008895 0.012452
Bud Site 0.000486 0.002663 0.001261 0.008574 0.000316 0.002506 0.008520 0.014994 0.000408 0.002535 0.005886 0.011759
Cell Periphery 0.000073 0.000212 0.000101 0.000180 0.000132 0.000115 0.000217 0.000169 0.000100 0.000133 0.000175 0.000174
Cytoplasm 0.094406 0.120996* 0.016555 0.088413 0.118860* 0.075440 0.005580 0.085147 0.105580* 0.083744 0.009563 0.086793
Cytoplasmic Foci 0.011085 0.011134 0.000771 0.003876 0.014526 0.007990 0.000465 0.002344 0.012657 0.008563 0.000576 0.003116
Eisosomes 0.000016 0.000032 0.000047 0.000027 0.000027 0.000017 0.000037 0.000030 0.000021 0.000020 0.000040 0.000028
Endoplasmic Reticulum 0.001161 0.004876 0.012165 0.007780 0.000794 0.001564 0.009464 0.007359 0.000994 0.002168 0.010444 0.007571
Endosome 0.000766 0.001383 0.000596 0.004215 0.000690 0.001924 0.001817 0.002546 0.000731 0.001825 0.001374 0.003387
Golgi 0.000868 0.000216 0.000069 0.005727 0.000220 0.001790 0.001656 0.002093 0.000572 0.001503 0.001080 0.003924
Lipid Particles 0.001285 0.001239 0.000582 0.000809 0.001765 0.001456 0.000336 0.000464 0.001504 0.001417 0.000425 0.000638
Mitochondria 0.002380 0.001350 0.000657 0.004793 0.000832 0.004770 0.001359 0.002854 0.001673 0.004147 0.001104 0.003831
Mitotic Spindle 0.003697 0.001289 0.000320 0.028463 0.000952 0.002765 0.000315 0.020460 0.002442 0.002496 0.000317 0.024493
None 0.003699 0.021544 0.001877 0.007839 0.008614 0.006860 0.001976 0.007426 0.005945 0.009536 0.001940 0.007634
Nuclear Periphery 0.001510 0.000869 0.000795 0.002450 0.000886 0.001162 0.000921 0.002012 0.001225 0.001109 0.000875 0.002233
Nuclear Periphery Foci 0.000231 0.000549 0.000471 0.003449 0.000182 0.000520 0.000184 0.000666 0.000209 0.000525 0.000288 0.002068
Nucleolus 0.001446 0.004730 0.001475 0.001105 0.001545 0.002540 0.001050 0.001334 0.001491 0.002939 0.001205 0.001219
Nucleus 0.871372* 0.812972* 0.948055* 0.786631* 0.844195* 0.878160* 0.945384* 0.806948* 0.858954* 0.866279* 0.946353* 0.796709*
Peroxisomes 0.000647 0.001590 0.000195 0.000761 0.000449 0.001414 0.000133 0.000326 0.000556 0.001446 0.000155 0.000545
Vacuole 0.002665 0.007322 0.009405 0.028311 0.003521 0.003664 0.007010 0.024392 0.003056 0.004331 0.007879 0.026367
Vacuole Periphery 0.000282 0.000352 0.000104 0.005356 0.000171 0.000485 0.000147 0.002355 0.000231 0.000461 0.000131 0.003867

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.50 -9.92 -4.39 -2.93 1.02 -1.49 -4.27 -5.89 -3.98 1.11 -2.10 -6.56 -6.87 -4.71 1.31
Bud Neck -3.21 -4.87 -11.15 -6.39 -5.64 -4.14 -4.83 -10.50 -7.13 -2.07 -4.60 -5.73 -14.98 -9.09 -3.37
Bud Site -2.48 -3.65 -6.27 -2.80 -3.38 -3.39 -1.93 -6.77 -4.93 -1.95 -3.85 -2.37 -9.02 -5.65 -2.36
Cell Periphery -4.09 -2.97 -7.23 2.07 -0.51 0.99 -0.21 -0.71 -2.40 0.03 -2.61 -0.94 -4.49 -0.92 -0.05
Cytoplasm -2.38 13.29 5.24 4.96 -8.50 7.83 24.74 7.33 -0.05 -17.95 5.22 28.76 8.78 2.83 -19.25
Cytoplasmic Foci -0.28 8.92 8.36 4.39 -1.64 4.31 11.23 9.94 7.00 -4.52 3.48 14.40 12.89 8.37 -4.39
Eisosomes -4.37 -7.16 -13.06 -2.68 2.71 2.98 -3.90 -5.57 -14.47 -0.09 0.65 -8.18 -12.03 -16.04 1.75
Endoplasmic Reticulum -6.32 -10.73 -21.07 -6.86 3.70 -5.44 -13.87 -21.83 -19.35 2.29 -7.22 -17.33 -30.24 -23.71 4.15
Endosome -1.85 -1.17 -3.46 -1.33 -2.03 -2.40 -0.71 -3.06 0.39 -0.71 -2.58 -0.96 -4.59 -0.08 -1.43
Golgi 2.00 2.39 -1.17 -2.18 -2.35 -3.02 -0.77 -1.27 1.44 -0.02 -2.03 0.07 -1.57 0.32 -1.21
Lipid Particles -0.14 1.42 1.52 2.59 0.14 0.98 6.32 5.12 2.28 -3.67 0.33 4.39 3.64 2.63 -2.53
Mitochondria 1.59 2.74 -0.60 -2.18 -3.23 -4.13 -1.27 -3.32 2.63 -2.36 -3.04 1.41 -1.84 1.91 -3.66
Mitotic Spindle 1.89 1.55 -5.61 -6.68 -6.46 -1.80 0.84 -6.06 -5.28 -6.43 -0.07 2.22 -8.13 -8.07 -9.30
None -5.14 3.36 -3.35 4.35 -5.37 1.29 6.01 2.27 1.19 -6.40 -3.44 7.11 -0.03 3.59 -8.43
Nuclear Periphery 1.65 0.80 -5.16 -12.48 -9.81 -1.30 -4.34 -15.52 -8.88 -8.89 0.44 -0.94 -10.03 -11.52 -12.82
Nuclear Periphery Foci -2.25 -2.84 -2.15 -1.61 -1.05 -1.82 -1.56 -6.75 -1.73 -5.35 -2.05 -3.09 -3.17 -2.00 -2.20
Nucleolus -2.55 -0.28 1.58 3.00 2.09 -2.70 2.98 1.84 3.58 -0.80 -3.46 1.36 2.21 4.90 0.86
Nucleus 4.39 -6.60 7.90 0.42 12.02 -4.62 -11.31 6.37 10.59 16.02 -1.31 -12.74 10.24 10.31 20.07
Peroxisomes -1.84 3.05 1.87 2.44 -0.49 -3.18 3.67 2.10 4.04 -2.01 -3.35 4.83 2.69 4.48 -1.49
Vacuole -4.04 -5.69 -15.52 -12.82 -9.20 -0.15 -4.25 -16.59 -16.47 -9.62 -2.32 -6.20 -22.72 -21.72 -12.62
Vacuole Periphery -0.47 1.22 -2.25 -2.17 -2.51 -2.90 0.83 -1.82 -1.07 -1.93 -2.17 1.45 -2.86 -2.26 -3.16
Standard name
Human Ortholog
Description Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (74%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Fzf1

Fzf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fzf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available