Standard name
Human Ortholog
Description Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05
Bud 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.78 0.63 0.69 0.67 0.5 0.56 0.5 0.5 0.49 0.42 0.35 0.79 0.84 0.85 0.38 0.32 0.27 0.28 0.4 0.36 0.33 0.38 0.3
Endoplasmic Reticulum 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.2 0.25 0.2 0.05 0.07 0 0.07 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.16 0.28 0.32 0.4 0.45 0.62 0.57 0.61 0.57 0.67 0.75 0.75 0.14 0.09 0.05 0.17 0.06 0.14 0.43 0.34 0.42 0.4 0.36 0.34
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.07 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0.16 0.2 0.2 0 0 0 0.05 0.05 0.07
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0.19 0.12 0.07 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 9 3 2 0 7 13 23
Bud 1 0 1 1 6 2 0 4 4 3 0 3 4 6 1 8 1 10 0 2 0 5 9 12
Bud Neck 0 0 1 0 0 0 2 1 2 0 0 0 0 1 1 2 2 0 2 2 0 2 6 20
Bud Site 1 0 0 0 1 0 0 2 1 0 0 0 0 0 0 0 1 2 – – – – – –
Cell Periphery 1 1 0 0 0 0 0 1 0 0 0 0 1 3 1 6 5 5 0 0 0 1 2 6
Cytoplasm 112 93 12 43 56 54 60 65 57 42 47 45 254 368 456 70 54 44 56 57 17 94 155 137
Endoplasmic Reticulum 0 1 2 0 1 0 0 2 0 0 0 1 3 1 1 12 7 11 7 0 0 3 2 7
Endosome 12 1 0 1 0 1 0 0 0 0 0 0 18 16 27 37 42 32 11 9 1 20 21 21
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 1 0 3 3 5 8 1 0 0 1 3 1
Mitochondria 25 33 6 25 38 68 62 79 66 58 85 95 45 38 29 32 10 22 87 49 20 116 149 158
Nucleus 1 1 0 0 3 1 0 1 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 1 1 1 0 1 0 1 0 0 0 0 0 2 0 0 0 2 0
Nucleolus 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 1 2 1
Peroxisomes 4 0 0 0 0 0 0 0 0 0 0 0 7 10 18 20 19 12 2 0 0 2 5 3
SpindlePole 7 0 0 0 2 3 4 11 7 2 5 4 5 5 4 30 34 33 3 4 1 15 20 31
Vac/Vac Membrane 8 3 0 2 1 1 2 3 4 3 0 1 16 10 15 16 32 20 14 7 1 9 11 26
Unique Cell Count 157 120 19 62 84 109 108 129 115 86 113 127 323 437 537 186 171 162 204 144 49 290 413 466
Labelled Cell Count 173 133 22 72 110 130 132 170 143 108 138 149 357 459 558 238 213 208 204 144 49 290 413 466


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.7 3.4 3.5 3.5 3.5 2.7 3.1 2.9 2.6 2.7 2.4 4.5 4.2 4.4 5.4 6.3 6.5 4.0 4.3 5.1
Std Deviation (1e-4) 2.8 1.0 0.8 1.4 1.3 1.5 1.2 1.4 1.3 1.8 1.1 1.1 1.5 1.1 1.7 1.9 2.1 2.0 1.5 2.1 1.9
Intensity Change (Log2) – – – 0.06 0.04 0.04 -0.34 -0.11 -0.22 -0.37 -0.3 -0.51 0.4 0.32 0.39 0.68 0.9 0.95 0.25 0.36 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7146 1.7178 1.296 1.6301 1.6273 1.961 2.2161 3.8062 2.3719 2.6172 2.3209 2.8229 1.8376 3.7141 2.5468 3.0914 3.4909 2.869
Actin 0.0724 0.0167 0.0119 0.0046 0.021 0.0207 0.0241 0.0408 0.0276 0.0189 0.0483 0.0174 0.0226 0.0194 0.0483 0.0094 0.0589 0.0237
Bud 0.005 0.0033 0.0022 0.0059 0.0202 0.0015 0.002 0.0138 0.0042 0.0068 0.0041 0.0017 0.0016 0.0052 0.0055 0.002 0.0031 0.0006
Bud Neck 0.006 0.0022 0.0072 0.001 0.0012 0.006 0.0088 0.0011 0.0018 0.0055 0.0026 0.004 0.0053 0.0025 0.0027 0.0055 0.0025 0.0021
Bud Periphery 0.0192 0.0091 0.0051 0.0085 0.0354 0.0047 0.0058 0.0141 0.0064 0.0184 0.0181 0.0066 0.0043 0.0192 0.0161 0.0063 0.0076 0.0016
Bud Site 0.0068 0.0408 0.014 0.0021 0.0086 0.0022 0.0287 0.0446 0.0119 0.0022 0.0035 0.0008 0.0134 0.0589 0.0138 0.0073 0.003 0.0007
Cell Periphery 0.004 0.007 0.0083 0.0008 0.0011 0.0011 0.0017 0.0027 0.0031 0.0005 0.0022 0.001 0.0096 0.0023 0.0056 0.0024 0.0011 0.0018
Cytoplasm 0.0595 0.1292 0.0821 0.1926 0.1627 0.0747 0.1301 0.2722 0.1179 0.2452 0.1332 0.1344 0.0693 0.0684 0.0822 0.0695 0.0665 0.0772
Cytoplasmic Foci 0.1109 0.0782 0.0579 0.0742 0.0761 0.106 0.0724 0.0926 0.0991 0.1215 0.1476 0.0798 0.1302 0.1167 0.0713 0.1246 0.0982 0.125
Eisosomes 0.0009 0.0008 0.0024 0.0003 0.0003 0.0006 0.0017 0.0007 0.0045 0.0008 0.0011 0.0005 0.0038 0.0013 0.0026 0.0014 0.0013 0.001
Endoplasmic Reticulum 0.0106 0.009 0.0141 0.0023 0.018 0.0064 0.008 0.001 0.0023 0.0072 0.0034 0.0024 0.0126 0.0013 0.0088 0.0018 0.0068 0.0023
Endosome 0.1325 0.1035 0.1159 0.0178 0.1718 0.0807 0.1072 0.0506 0.0584 0.0719 0.1351 0.103 0.082 0.0715 0.0949 0.0889 0.0768 0.0656
Golgi 0.0509 0.0547 0.0552 0.0156 0.0578 0.0629 0.047 0.0525 0.0554 0.0169 0.0883 0.0728 0.0304 0.0786 0.0587 0.0358 0.0947 0.094
Lipid Particles 0.0538 0.0094 0.022 0.0073 0.0148 0.04 0.0309 0.0125 0.0061 0.0033 0.0145 0.0204 0.04 0.0251 0.0182 0.0113 0.0214 0.0249
Mitochondria 0.2529 0.4184 0.4491 0.4915 0.2835 0.4096 0.2755 0.2764 0.3235 0.2599 0.2441 0.405 0.2652 0.2917 0.3672 0.3699 0.3866 0.3729
None 0.1285 0.0682 0.1024 0.1616 0.071 0.1111 0.1902 0.1 0.1895 0.1391 0.0924 0.0802 0.1789 0.1375 0.1463 0.1486 0.1332 0.1225
Nuclear Periphery 0.0044 0.001 0.0018 0.0006 0.0021 0.0016 0.0067 0.0003 0.0004 0.0067 0.0008 0.0006 0.0033 0.0005 0.0012 0.0006 0.0037 0.0004
Nucleolus 0.0007 0.0011 0.001 0.0002 0.0007 0.001 0.0014 0.0008 0.0005 0.0003 0.0005 0.0004 0.0041 0.0007 0.0005 0.001 0.0005 0.0003
Nucleus 0.0013 0.0015 0.0011 0.0012 0.0016 0.0114 0.0026 0.0011 0.0006 0.0019 0.0007 0.0006 0.0065 0.0008 0.0011 0.0008 0.0013 0.0005
Peroxisomes 0.0606 0.0301 0.0321 0.0047 0.0246 0.047 0.0315 0.0171 0.081 0.0639 0.0492 0.0567 0.0811 0.0925 0.0433 0.1011 0.0211 0.074
Punctate Nuclear 0.0094 0.0004 0.0006 0.0016 0.0011 0.0011 0.0043 0.0006 0.0005 0.0011 0.0007 0.0005 0.007 0.0006 0.0008 0.003 0.004 0.0013
Vacuole 0.0066 0.0119 0.009 0.0038 0.0207 0.0059 0.0134 0.0034 0.0039 0.0048 0.0063 0.0074 0.0247 0.0037 0.0075 0.0059 0.0042 0.0049
Vacuole Periphery 0.0031 0.0034 0.0045 0.0017 0.0057 0.0038 0.006 0.0011 0.0015 0.0033 0.0031 0.0035 0.0042 0.0015 0.0036 0.003 0.0035 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.2143 27.4709 26.7548 24.1141 21.1666 21.1153 28.4268 28.4131 25.6874 28.5909
Translational Efficiency 0.6482 1.1152 0.5815 0.6174 0.7058 1.4569 0.8031 0.4557 0.5259 0.4783

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1295 1443 2215 707 1071 1458 179 1107 2366 2901 2394 1814

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 636.98 681.78 948.94 902.68 691.25 644.83 1001.54 889.63 661.55 663.21 952.87 894.72
Standard Deviation 87.87 109.15 117.62 131.20 74.63 95.55 141.48 150.54 86.47 104.19 120.37 143.46
Intensity Change Log 2 — 0.098058 0.575069 0.502967 — -0.100289 0.534941 0.363998 — -0.001761 0.554324 0.432315

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000221 0.001617 0.001247 0.017153 0.000329 0.001925 0.007433 0.014563 0.000270 0.001772 0.001710 0.015572
Bud Neck 0.018834 0.055577 0.020284 0.018494 0.028777 0.061868 0.011179 0.020008 0.023335 0.058739 0.019604 0.019418
Bud Site 0.007190 0.039434 0.061772 0.144471* 0.010085 0.048100 0.044724 0.153360* 0.008501 0.043789 0.060497 0.149895*
Cell Periphery 0.000277 0.000265 0.000115 0.000566 0.000212 0.000353 0.000248 0.000462 0.000248 0.000309 0.000125 0.000503
Cytoplasm 0.394343* 0.158096* 0.323935* 0.172166* 0.322436* 0.149590* 0.144004* 0.172176* 0.361794* 0.153821* 0.310482* 0.172172*
Cytoplasmic Foci 0.227097* 0.284715* 0.151106 0.137775 0.306227* 0.265797* 0.108302 0.122733 0.262916* 0.275207* 0.147905 0.128595
Eisosomes 0.000190 0.000254 0.000034 0.000144 0.000186 0.000257 0.000147 0.000152 0.000189 0.000256 0.000043 0.000149
Endoplasmic Reticulum 0.000935 0.000843 0.002009 0.002281 0.000731 0.000700 0.006603 0.001771 0.000843 0.000771 0.002353 0.001970
Endosome 0.009358 0.071041 0.051898 0.051786 0.028271 0.070756 0.076478 0.039747 0.017919 0.070898 0.053736 0.044439
Golgi 0.010026 0.044491 0.041488 0.049443 0.020833 0.043617 0.015143 0.054024 0.014918 0.044052 0.039518 0.052239
Lipid Particles 0.015072 0.014265 0.001728 0.002630 0.014160 0.017123 0.011156 0.002244 0.014659 0.015701 0.002433 0.002394
Mitochondria 0.070284 0.131740* 0.045250 0.067208 0.076963 0.153212* 0.046987 0.097508 0.073308 0.142532* 0.045380 0.085699
Mitotic Spindle 0.004051 0.012440 0.084088 0.073033 0.005801 0.015719 0.106596* 0.082940 0.004843 0.014088 0.085771 0.079079
None 0.010964 0.003211 0.001779 0.002080 0.005379 0.003635 0.000924 0.001600 0.008436 0.003424 0.001715 0.001787
Nuclear Periphery 0.000516 0.000300 0.000796 0.000449 0.000422 0.000463 0.003213 0.000321 0.000473 0.000382 0.000977 0.000371
Nuclear Periphery Foci 0.000509 0.000345 0.001084 0.000993 0.000369 0.000247 0.004101 0.000695 0.000445 0.000296 0.001310 0.000811
Nucleolus 0.002489 0.003427 0.001647 0.000916 0.003694 0.002714 0.004599 0.000518 0.003035 0.003069 0.001868 0.000673
Nucleus 0.189482* 0.038837 0.052361 0.033961 0.108271 0.033006 0.126715 0.031599 0.152720* 0.035906 0.057920 0.032519
Peroxisomes 0.023651 0.072969 0.017360 0.093748 0.030251 0.069190 0.090005 0.107474* 0.026639 0.071070 0.022791 0.102124*
Vacuole 0.011795 0.057363 0.122473 0.124952 0.031506 0.053852 0.180337 0.089959 0.020717 0.055598 0.126799 0.103597
Vacuole Periphery 0.002714 0.008773 0.017543 0.005751 0.005097 0.007876 0.011108 0.006147 0.003793 0.008322 0.017062 0.005993

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.46 -6.98 -7.51 -6.64 -6.94 -3.34 -2.63 -8.52 -7.07 -2.43 -4.80 -6.27 -11.37 -9.74 -10.02
Bud Neck -10.29 -1.05 0.75 11.32 2.14 -8.22 6.20 2.91 11.49 -4.17 -13.24 1.61 2.25 15.86 0.78
Bud Site -12.90 -20.18 -15.65 -10.90 -8.27 -11.94 -3.92 -19.80 -12.63 -10.01 -17.39 -20.64 -25.32 -16.95 -13.79
Cell Periphery 0.39 5.56 -1.06 -1.13 -1.97 -3.24 -0.79 -4.29 -1.10 -3.38 -2.03 5.05 -2.44 -1.63 -3.99
Cytoplasm 23.94 6.00 16.53 -2.83 12.20 16.69 9.56 11.19 -4.65 -2.36 29.00 4.97 20.30 -5.32 14.44
Cytoplasmic Foci -6.75 11.56 12.02 19.54 2.53 4.21 19.02 22.54 20.86 -1.72 -2.00 20.66 24.32 29.06 4.41
Eisosomes -2.68 14.67 3.57 4.92 -14.79 -3.61 2.00 3.05 6.61 0.56 -4.36 18.35 4.65 7.89 -18.83
Endoplasmic Reticulum 0.37 -3.94 -5.47 -7.55 -2.41 0.25 -5.00 -6.91 -6.73 4.02 0.49 -7.05 -7.70 -10.06 0.58
Endosome -19.48 -19.00 -10.19 6.35 1.61 -12.25 -4.10 -2.24 10.20 3.31 -22.81 -15.05 -9.73 11.99 4.65
Golgi -12.84 -11.54 -6.51 1.24 -0.10 -7.30 2.84 -6.09 -0.23 -7.92 -14.33 -9.32 -9.59 0.60 -1.89
Lipid Particles 0.53 10.38 8.68 8.78 -4.00 -1.52 1.82 10.32 9.15 4.34 -0.73 13.97 13.27 12.74 -1.80
Mitochondria -7.68 4.84 2.21 9.30 -1.58 -8.89 3.37 -0.49 8.36 -3.68 -11.80 6.88 0.62 11.87 -5.68
Mitotic Spindle -4.83 -20.17* -9.68 -8.34 2.11 -4.61 -5.76 -13.23 -10.73 1.16 -6.65 -21.14 -16.46 -13.80 2.21
None 5.52 7.52 6.01 1.41 -0.13 1.86 5.25 3.90 2.24 -0.37 5.71 8.94 7.32 2.61 0.01
Nuclear Periphery 1.87 -2.90 -1.13 -3.98 1.96 -0.32 -2.43 -1.35 -0.32 2.36 1.03 -4.75 -1.27 -2.48 4.30
Nuclear Periphery Foci 0.63 -2.74 -3.65 -5.88 -1.85 3.06 -6.52 -4.00 -4.83 5.47 1.12 -5.99 -4.55 -7.39 1.25
Nucleolus -1.47 1.69 3.28 4.08 2.89 1.09 -0.18 3.73 4.13 3.55 -0.05 2.53 5.08 5.93 6.10
Nucleus 22.79 20.19 19.09 -1.95 1.83 14.15 -2.15 10.92 -2.90 5.89 26.45 18.45 22.08 -3.30 4.79
Peroxisomes -12.23 3.00 -8.54 -0.77 -10.01 -9.65 -3.20 -10.25 -3.62 -1.40 -15.61 2.75 -13.59 -3.34 -15.04
Vacuole -15.93 -32.94 -19.13 -13.47 -5.39 -7.18 -10.65 -16.38 -12.35 4.66 -16.58 -31.95 -25.55 -18.06 -2.67
Vacuole Periphery -8.28 -12.47 -2.44 4.47 9.47 -3.32 -1.81 -0.08 2.60 1.68 -8.30 -11.66 -1.71 4.63 9.52
Standard name
Human Ortholog
Description Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
Localization
Cell Percentages mitochondrion (14%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Adh4

Adh4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adh4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available