Standard name
Human Ortholog
Description Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.79 0.67 0.65 0.81 0.72 0.69 – 0.54 0.62 0.47 0.58 – – – 0.52 0.46 0.45 0.66 0.59 0.54 0.4 0.35 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.14 0.23 – 0.46 0.37 0.53 0.29 – – – 0 0 0 0 0 0 0 0 0.14
Nucleus 0 0.08 0.1 0.07 0.06 0.11 0.12 – 0.1 0.09 0.1 0.09 – – – 0.18 0.1 0.15 0.18 0.21 0.17 0.25 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.26 0.21 0.27 0.28 0.19 0.15 0.11 – 0.1 0.1 0.1 0.13 – – – 0.42 0.52 0.49 0.07 0.14 0.18 0.35 0.62 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 1 – – – 0 0 0 0 0 1 0 0 0
Bud 0 2 0 2 1 1 1 – 6 5 7 4 – – – 0 0 0 0 2 4 0 0 0
Bud Neck 0 0 4 1 0 0 5 – 0 2 1 3 – – – 0 0 0 0 3 2 0 0 0
Bud Site 0 0 0 0 0 1 1 – 1 1 2 3 – – – 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 2 0 0 – 2 2 1 3 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 55 250 151 132 221 246 332 – 240 349 205 248 – – – 131 73 57 77 234 159 2 2 2
Endoplasmic Reticulum 0 4 1 0 0 1 1 – 0 0 1 9 – – – 10 3 4 1 0 1 0 0 0
Endosome 0 1 0 6 1 2 0 – 2 4 2 2 – – – 15 7 5 3 3 1 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Mitochondria 6 5 0 9 4 46 113 – 204 209 231 124 – – – 3 4 1 0 2 10 0 0 0
Nucleus 0 24 23 15 16 37 57 – 46 49 44 38 – – – 45 16 19 20 82 49 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 0 2 3 0 – – – 0 1 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 1 1 1 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 0 – 1 3 1 4 – – – 2 0 0 1 4 6 0 0 0
Vac/Vac Membrane 20 66 61 57 52 51 51 – 46 54 45 54 – – – 106 83 62 8 53 54 2 4 1
Unique Cell Count 76 318 225 204 272 340 482 443 565 440 426 254 159 126 118 395 297 7 7 5
Labelled Cell Count 83 353 241 222 297 387 561 549 682 544 493 313 187 148 118 395 297 7 7 5


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.0 5.8 5.3 5.6 4.4 4.4 – 4.0 3.9 3.9 4.1 – – – 6.9 7.3 6.9 – – –
Std Deviation (1e-4) 0.6 0.6 1.0 1.1 1.3 0.8 0.8 – 1.1 0.8 0.9 0.5 – – – 1.2 1.3 1.1 – – –
Intensity Change (Log2) – – – -0.13 -0.04 -0.39 -0.39 – -0.52 -0.57 -0.58 -0.49 – – – 0.25 0.34 0.26 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm -0.5 3.6 1.3 0.5 – -3.2 -1.4 -5.0 -2.2 – – – -3.4 -4.2 -4.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 2.5 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 3.7 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleus -1.0 -1.8 0.2 0.6 – 0.1 -0.7 -0.1 -0.5 – – – 2.3 -0.1 1.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0.2 -2.1 -3.5 -5.6 – -5.6 -6.3 -5.6 -4.6 – – – 3.4 5.0 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4483 0.3797 0.077 -0.3018 0.1209 0.186 0.1209 0.4481 0.2602 0.2592 0.0671 0.2712 -0.9808 -0.1467 -0.9175 0.1626 -0.127 -1.2248
Actin 0.0584 0.0005 0.0002 0.0001 0.0047 0.0134 0.0219 0.0039 0.0132 0.005 0.0007 0.0094 0.0345 0.0006 0.0004 0.0416 0.0322 0.0011
Bud 0.0004 0.0004 0 0 0.0007 0.0003 0.002 0.0015 0.0035 0.0003 0.0001 0.0003 0.0004 0.0001 0.0001 0.0001 0.0006 0
Bud Neck 0.0091 0.0033 0.0001 0.0001 0.0001 0.0016 0.0023 0.0002 0.0005 0.0009 0.0004 0.0018 0.0171 0.0003 0.0003 0.0008 0.0008 0.0003
Bud Periphery 0.0007 0.0003 0 0 0.001 0.0007 0.0028 0.0009 0.0065 0.0003 0 0.0003 0.0006 0.0001 0 0.0001 0.0005 0
Bud Site 0.006 0.0234 0 0 0.0002 0.0002 0.0146 0.0023 0.0047 0.0008 0.0002 0.0003 0.003 0.0018 0.0003 0.0002 0.0037 0
Cell Periphery 0.002 0.0002 0 0 0.0003 0.0001 0.0004 0.0001 0.0002 0.0001 0 0 0.0004 0.0002 0.0001 0.0001 0.0002 0
Cytoplasm 0.1867 0.3979 0.4491 0.2351 0.3093 0.4305 0.4327 0.6213 0.6063 0.491 0.5991 0.6729 0.3812 0.4613 0.4864 0.5715 0.2466 0.4529
Cytoplasmic Foci 0.0291 0.0079 0.0061 0.0047 0.0045 0.0348 0.0662 0.0102 0.0239 0.017 0.0118 0.0171 0.0367 0.012 0.0046 0.0045 0.0099 0.0064
Eisosomes 0.0049 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0002 0.0002 0 0 0.001 0.0002 0.0005 0.0003 0.0002 0.0005
Endoplasmic Reticulum 0.0138 0.0048 0.0026 0.0053 0.0095 0.0079 0.0069 0.0038 0.0055 0.0035 0.0048 0.006 0.0058 0.0096 0.0035 0.0058 0.0168 0.003
Endosome 0.028 0.0042 0.0015 0.0043 0.0024 0.0289 0.0556 0.0034 0.0263 0.0472 0.042 0.0329 0.0111 0.0059 0.0033 0.004 0.0581 0.0038
Golgi 0.0084 0.0005 0.0001 0.0001 0.0005 0.0221 0.0148 0.001 0.0079 0.014 0.0027 0.0097 0.0065 0.0004 0.0001 0.0081 0.0082 0.0001
Lipid Particles 0.0813 0.0181 0.0005 0.0003 0.0006 0.0035 0.0339 0.0016 0.0032 0.0315 0.0062 0.0022 0.0087 0.002 0.0006 0.0015 0.0064 0.0006
Mitochondria 0.0059 0.0004 0.0002 0.0002 0.0002 0.0023 0.0106 0.0007 0.0108 0.0666 0.0006 0.0044 0.0016 0.0002 0.0004 0.0016 0.0034 0.0002
None 0.4769 0.5155 0.526 0.7417 0.6581 0.3833 0.2378 0.3054 0.232 0.2486 0.3097 0.1537 0.4386 0.4519 0.4609 0.3091 0.5675 0.4972
Nuclear Periphery 0.0046 0.0029 0.0025 0.002 0.0026 0.0083 0.0218 0.0033 0.0049 0.0045 0.0032 0.0091 0.0067 0.0047 0.0035 0.0034 0.0238 0.0038
Nucleolus 0.0072 0.0005 0.0001 0.0002 0.0001 0.0004 0.0015 0.0003 0.0003 0.0006 0.0002 0.0004 0.0006 0.0003 0.0012 0.0001 0.0007 0.0004
Nucleus 0.0066 0.0084 0.0071 0.0028 0.0026 0.0321 0.021 0.0321 0.0347 0.0415 0.0107 0.0619 0.0244 0.0431 0.0271 0.04 0.0067 0.0233
Peroxisomes 0.0367 0.0049 0.0003 0.0001 0.0001 0.0012 0.0344 0.0022 0.0092 0.0179 0.0005 0.0006 0.0142 0.0003 0.0001 0.0004 0.0021 0.0003
Punctate Nuclear 0.0111 0.0043 0.0022 0.0011 0.0008 0.0218 0.0121 0.0035 0.0031 0.0026 0.0032 0.0134 0.0043 0.0033 0.0055 0.0056 0.007 0.0049
Vacuole 0.0214 0.0014 0.001 0.0015 0.0015 0.0042 0.0047 0.0019 0.0026 0.0043 0.0032 0.0027 0.0021 0.0016 0.0012 0.0009 0.0027 0.001
Vacuole Periphery 0.0009 0.0002 0.0002 0.0001 0.0004 0.0024 0.0016 0.0003 0.0006 0.0016 0.0008 0.0008 0.0005 0.0002 0.0002 0.0002 0.0017 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.418 11.916 7.8597 13.3284 8.6413 11.3487 14.614 12.6494 12.1839 15.2759
Translational Efficiency 1.1193 0.6917 0.917 0.561 0.8548 0.5525 0.6156 0.5814 0.7427 0.7002

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1172 989 149 1211 1654 1515 1808 1704 2826 2504 1957 2915

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.24 778.82 962.37 819.37 712.26 807.82 919.45 824.95 695.66 796.37 922.72 822.63
Standard Deviation 75.30 104.49 110.96 127.72 85.23 104.23 118.22 118.53 83.62 105.29 118.23 122.46
Intensity Change Log 2 — 0.212314 0.517615 0.285539 — 0.181630 0.368367 0.211903 — 0.196610 0.442764 0.248126

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000084 0.000220 0.001218 0.000732 0.000103 0.000355 0.000349 0.002327 0.000095 0.000302 0.000415 0.001665
Bud Neck 0.002142 0.007756 0.003134 0.012834 0.003327 0.012201 0.008334 0.005212 0.002836 0.010445 0.007938 0.008379
Bud Site 0.001523 0.005532 0.002467 0.016912 0.001662 0.006624 0.011800 0.013412 0.001604 0.006193 0.011090 0.014866
Cell Periphery 0.000139 0.000137 0.000233 0.000738 0.000141 0.000118 0.000130 0.000278 0.000140 0.000125 0.000138 0.000469
Cytoplasm 0.359244* 0.473612* 0.097850 0.294932* 0.450510* 0.402275* 0.205502* 0.333554* 0.412660* 0.430451* 0.197306* 0.317509*
Cytoplasmic Foci 0.040301 0.082107 0.003639 0.025921 0.097625 0.107309 0.001942 0.005809 0.073851 0.097355 0.002071 0.014165
Eisosomes 0.000040 0.000053 0.000088 0.000386 0.000066 0.000065 0.000024 0.000033 0.000055 0.000060 0.000029 0.000179
Endoplasmic Reticulum 0.000706 0.001869 0.016747 0.002407 0.001237 0.001416 0.004617 0.002822 0.001017 0.001595 0.005540 0.002650
Endosome 0.001067 0.005136 0.002394 0.013699 0.003277 0.006425 0.001204 0.001549 0.002360 0.005916 0.001295 0.006596
Golgi 0.000252 0.000700 0.000130 0.007298 0.001219 0.003799 0.000150 0.000197 0.000818 0.002575 0.000148 0.003147
Lipid Particles 0.002098 0.003046 0.002578 0.006834 0.005846 0.004778 0.000649 0.000685 0.004292 0.004094 0.000796 0.003240
Mitochondria 0.001420 0.001297 0.000870 0.008600 0.003511 0.008917 0.001397 0.002212 0.002644 0.005907 0.001357 0.004866
Mitotic Spindle 0.000352 0.001927 0.000329 0.021420 0.002802 0.009726 0.020258 0.011696 0.001786 0.006646 0.018740 0.015736
None 0.014346 0.005294 0.002513 0.009315 0.004731 0.003363 0.010322 0.015133 0.008718 0.004126 0.009727 0.012716
Nuclear Periphery 0.000382 0.000853 0.002060 0.001230 0.000811 0.000931 0.002547 0.001558 0.000633 0.000900 0.002510 0.001422
Nuclear Periphery Foci 0.000337 0.001444 0.002420 0.000840 0.000622 0.000549 0.000789 0.000590 0.000504 0.000903 0.000913 0.000694
Nucleolus 0.001501 0.001675 0.000911 0.002727 0.001119 0.001820 0.000641 0.001045 0.001277 0.001763 0.000661 0.001744
Nucleus 0.566443* 0.368609* 0.810345* 0.506147* 0.400027* 0.383550* 0.701885* 0.567114* 0.469043* 0.377649* 0.710142* 0.541786*
Peroxisomes 0.000944 0.002476 0.000362 0.003474 0.001427 0.006034 0.000212 0.000398 0.001227 0.004628 0.000224 0.001676
Vacuole 0.005988 0.035703 0.049427 0.060295 0.019315 0.038853 0.026974 0.033917 0.013788 0.037609 0.028684 0.044875
Vacuole Periphery 0.000692 0.000553 0.000284 0.003259 0.000623 0.000893 0.000275 0.000459 0.000652 0.000759 0.000276 0.001622

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.03 -8.46 -4.58 -3.60 3.40 -2.48 -10.61 -2.24 -1.79 -1.54 -3.30 -12.64 -3.03 -2.39 -1.61
Bud Neck -3.99 -2.18 -6.41 -2.18 -5.69 -7.07 -6.86 -5.38 4.21 2.47 -8.05 -7.60 -8.29 1.56 -0.35
Bud Site -3.35 -2.63 -6.45 -3.81 -5.64 -5.45 -4.81 -5.30 -1.40 -0.82 -6.31 -4.95 -8.30 -3.64 -2.49
Cell Periphery -0.08 -5.26 -5.83 -5.78 -3.42 2.19 1.52 -1.81 -2.95 -2.62 1.77 0.74 -5.83 -6.45 -6.13
Cytoplasm -8.71 18.82 11.98 20.65 -10.38 5.35 37.07 22.27 15.81 -12.15 -1.56 38.98 24.53 25.18 -14.20
Cytoplasmic Foci -9.25 16.58 9.32 15.53 -9.05 -1.81 29.23 28.17 25.81 -6.96 -6.48 33.06 28.35 29.80 -12.53
Eisosomes -4.38 -5.90 -3.76 -3.46 -2.38 0.17 6.39 5.05 6.36 -2.47 -1.83 3.94 -3.12 -2.84 -3.69
Endoplasmic Reticulum -5.38 -11.61 -15.29 -9.57 8.25 -1.09 -17.59 -13.58 -13.25 5.15 -4.15 -22.12 -20.27 -16.11 6.92
Endosome -6.49 -5.17 -5.06 -1.62 -3.69 -5.55 6.68 6.53 9.39 -0.61 -8.49 4.23 -3.08 2.69 -4.60
Golgi -2.16 1.39 -3.53 -3.05 -3.67 -2.44 2.95 2.89 3.81 -0.49 -2.74 3.17 -1.72 1.23 -3.70
Lipid Particles -3.02 -1.79 -2.18 -1.17 -1.22 1.46 8.09 8.17 8.50 0.78 0.46 8.81 3.69 3.58 -2.99
Mitochondria 0.26 0.90 -3.27 -3.83 -4.22 -2.96 2.23 1.97 4.39 -0.11 -2.88 1.95 -0.70 2.47 -3.21
Mitotic Spindle -2.76 -0.55 -7.19 -6.50 -7.16 -3.12 -6.96 -4.27 -0.38 3.66 -3.63 -7.56 -8.22 -4.38 1.65
None 6.47 9.21 4.55 -1.86 -5.32 2.50 -3.75 -5.59 -6.81 -2.49 6.90 1.92 -0.38 -6.63 -2.16
Nuclear Periphery -7.47 -11.90 -16.44 -11.64 -1.06 -2.38 -22.88 -18.42 -16.07 8.50 -6.49 -25.43 -24.62 -19.53 8.39
Nuclear Periphery Foci -3.89 -4.24 -6.87 0.62 2.44 0.55 -4.29 -3.18 -3.55 2.13 -3.49 -5.93 -7.07 -1.05 1.99
Nucleolus -0.88 3.31 -0.73 -0.13 -2.52 -2.20 1.72 1.30 2.54 -0.39 -2.34 2.96 -0.09 2.09 -2.16
Nucleus 16.10 -11.84 2.09 -13.70 13.13 1.77 -34.61 -22.69 -23.37 10.01 11.67 -31.09 -14.88 -26.45 16.36
Peroxisomes -3.39 1.92 -1.45 0.84 -2.56 -6.72 8.49 7.51 8.66 -1.41 -7.50 7.00 1.01 7.44 -3.02
Vacuole -12.78 -7.88 -20.87 -12.15 -4.55 -9.53 -13.20 -14.62 -7.18 -2.28 -15.63 -16.74 -24.99 -13.74 -7.08
Vacuole Periphery 0.28 0.83 -1.72 -2.95 -3.66 -1.22 2.68 1.88 3.79 -1.40 -0.41 2.05 -0.92 -0.82 -4.06
Standard name
Human Ortholog
Description Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
Localization
Cell Percentages cytoplasm (28%), nucleus (11%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cul3

Cul3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cul3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available