Standard name | |
---|---|
Human Ortholog | |
Description | Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 0 | 3 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | – | 2 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 6 | 10 | 4 | 6 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 17 | 14 | 17 | 28 | 24 | 57 | 74 | 130 | – | 146 | 147 | 203 | 75 | 75 | 133 | 51 | 80 | 45 | 1 | 1 | 2 | 2 | 3 | 2 |
Endoplasmic Reticulum | 1 | 1 | 3 | 1 | 3 | 5 | 2 | 3 | – | 2 | 4 | 2 | 1 | 2 | 4 | 8 | 16 | 12 | 1 | 2 | 0 | 2 | 2 | 2 |
Endosome | 16 | 14 | 11 | 3 | 16 | 1 | 5 | 1 | – | 0 | 0 | 0 | 13 | 20 | 7 | 10 | 12 | 8 | 9 | 10 | 7 | 19 | 19 | 34 |
Golgi | 2 | 5 | 1 | 0 | 3 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 3 | 3 | 1 | 2 | 1 | 3 | 3 |
Mitochondria | 14 | 46 | 36 | 16 | 6 | 39 | 47 | 49 | – | 57 | 41 | 45 | 1 | 1 | 0 | 14 | 29 | 5 | 8 | 4 | 4 | 6 | 9 | 6 |
Nucleus | 83 | 86 | 100 | 116 | 56 | 157 | 189 | 231 | – | 162 | 103 | 143 | 12 | 12 | 10 | 140 | 180 | 70 | 8 | 9 | 5 | 7 | 6 | 2 |
Nuclear Periphery | 6 | 5 | 9 | 1 | 0 | 0 | 1 | 3 | – | 1 | 2 | 3 | 0 | 0 | 1 | 5 | 4 | 3 | 6 | 2 | 5 | 4 | 9 | 12 |
Nucleolus | 12 | 13 | 6 | 0 | 2 | 1 | 4 | 5 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 4 | 3 | 0 | 0 | 0 | 1 |
Peroxisomes | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | – | 2 | 1 | 0 | 5 | 3 | 1 | 5 | 10 | 0 | 2 | 1 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 255 | 288 | 188 | 214 | 263 | 326 | 403 | 610 | – | 492 | 356 | 429 | 70 | 63 | 45 | 137 | 151 | 82 | 352 | 398 | 321 | 206 | 272 | 248 |
Unique Cell Count | 360 | 401 | 316 | 301 | 333 | 489 | 607 | 848 | 698 | 521 | 648 | 158 | 154 | 189 | 314 | 408 | 192 | 410 | 448 | 360 | 262 | 334 | 331 | |
Labelled Cell Count | 408 | 476 | 372 | 379 | 375 | 587 | 728 | 1041 | 868 | 658 | 829 | 181 | 180 | 209 | 378 | 487 | 231 | 410 | 448 | 360 | 262 | 334 | 331 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.3 | 10.8 | 9.6 | 9.9 | 13.5 | 12.5 | 12.5 | 12.4 | – | 11.1 | 10.9 | 10.0 | 7.2 | 7.2 | 6.7 | 8.3 | 8.2 | 10.0 | 10.4 | 11.4 | 11.9 |
Std Deviation (1e-4) | 5.7 | 4.1 | 3.7 | 3.8 | 4.8 | 5.9 | 6.4 | 6.1 | – | 5.6 | 5.6 | 4.5 | 2.4 | 2.4 | 1.9 | 3.3 | 4.2 | 4.3 | 4.7 | 4.8 | 4.7 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.49 | 0.38 | 0.38 | 0.37 | – | 0.22 | 0.18 | 0.06 | -0.4 | -0.4 | -0.53 | -0.21 | -0.23 | 0.06 | 0.12 | 0.24 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 1.0 | 3.0 | 3.3 | 4.5 | – | 6.2 | 8.1 | 9.0 | 10.9 | 11.1 | 15.5 | 4.4 | 5.6 | 6.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 2.5 | 3.4 |
Endosome | -2.1 | 0.8 | 0 | -2.9 | 0 | – | 0 | 0 | 0 | 2.2 | 3.9 | 0.1 | -0.2 | -0.4 | 0.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.7 | -5.0 | -1.6 | -1.8 | -3.3 | – | -1.6 | -1.7 | -2.3 | -4.1 | -4.1 | -4.8 | -3.2 | -2.0 | -3.5 |
Nucleus | 1.8 | -4.4 | 0.1 | -0.2 | -1.5 | – | -2.8 | -3.9 | -3.2 | -5.8 | -5.7 | -6.9 | 3.3 | 3.4 | 1.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | -1.9 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.0 | 5.4 | 2.1 | 2.1 | 4.1 | – | 3.4 | 2.6 | 2.0 | -3.1 | -3.8 | -7.8 | -4.0 | -6.0 | -3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.6146 | 9.1505 | 7.6919 | 7.4673 | 6.8805 | 8.4311 | 6.8343 | 12.6542 | 10.4373 | 10.383 | 11.0128 | 11.1833 | 6.9879 | 11.3743 | 9.3325 | 9.8968 | 9.2704 | 10.0943 |
Actin | 0.0062 | 0 | 0.0017 | 0.0001 | 0.0288 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0016 | 0.0002 | 0.0015 | 0 |
Bud | 0.0004 | 0.0012 | 0.0004 | 0.0002 | 0.0025 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0008 | 0.0008 | 0.0002 | 0.0003 |
Bud Neck | 0.001 | 0.0011 | 0.0012 | 0.0005 | 0.0015 | 0.0012 | 0.0007 | 0.0008 | 0.0007 | 0.0008 | 0.0007 | 0.0006 | 0.0004 | 0.0012 | 0.0036 | 0.0016 | 0.0005 | 0.0012 |
Bud Periphery | 0.0009 | 0.0028 | 0.001 | 0.0006 | 0.0033 | 0.0014 | 0.0008 | 0.0012 | 0.001 | 0.0005 | 0.001 | 0.0011 | 0.0008 | 0.0016 | 0.0028 | 0.002 | 0.0005 | 0.0014 |
Bud Site | 0.0041 | 0.0015 | 0.0014 | 0.0004 | 0.0186 | 0.001 | 0.0005 | 0.0009 | 0.0006 | 0.0004 | 0.0005 | 0.0003 | 0.0004 | 0.0014 | 0.0048 | 0.0016 | 0.0005 | 0.0004 |
Cell Periphery | 0.0064 | 0.0109 | 0.0067 | 0.0057 | 0.0031 | 0.0092 | 0.0073 | 0.013 | 0.0105 | 0.0065 | 0.0088 | 0.0107 | 0.005 | 0.0246 | 0.0124 | 0.0076 | 0.0105 | 0.0141 |
Cytoplasm | 0.009 | 0.0014 | 0.0036 | 0.0042 | 0.0013 | 0.0011 | 0.0007 | 0.001 | 0.0007 | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0018 | 0.0011 | 0.0006 | 0.0005 | 0.0004 |
Cytoplasmic Foci | 0.017 | 0.0023 | 0.0072 | 0.0025 | 0.0145 | 0.0015 | 0.0018 | 0.0029 | 0.0014 | 0.0016 | 0.0007 | 0.0005 | 0.0122 | 0.0082 | 0.0204 | 0.0071 | 0.0363 | 0.001 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0037 | 0.0017 | 0.0027 | 0.0013 | 0.0014 | 0.0015 | 0.0017 | 0.0023 | 0.0023 | 0.0007 | 0.0006 | 0.0016 | 0.0007 | 0.006 | 0.0016 | 0.0007 | 0.0005 | 0.0012 |
Endosome | 0.0961 | 0.0332 | 0.106 | 0.0499 | 0.0782 | 0.0459 | 0.0768 | 0.037 | 0.0747 | 0.067 | 0.0493 | 0.0405 | 0.1454 | 0.0643 | 0.1072 | 0.0878 | 0.1234 | 0.0399 |
Golgi | 0.009 | 0.0029 | 0.0091 | 0.0036 | 0.0191 | 0.0018 | 0.0035 | 0.0015 | 0.0034 | 0.0016 | 0.002 | 0.0017 | 0.0075 | 0.0073 | 0.0266 | 0.0133 | 0.0829 | 0.003 |
Lipid Particles | 0.0287 | 0.0058 | 0.0211 | 0.0386 | 0.008 | 0.0087 | 0.0136 | 0.0049 | 0.0119 | 0.0062 | 0.0042 | 0.0069 | 0.0658 | 0.0653 | 0.0646 | 0.1059 | 0.0708 | 0.0546 |
Mitochondria | 0.0096 | 0.0033 | 0.0073 | 0.0273 | 0.0322 | 0.0025 | 0.018 | 0.0035 | 0.0217 | 0.002 | 0.0126 | 0.0131 | 0.0619 | 0.0236 | 0.0821 | 0.0193 | 0.0308 | 0.0049 |
None | 0.0012 | 0.0002 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0118 | 0.0031 | 0.0043 | 0.0031 | 0.0084 | 0.0048 | 0.0043 | 0.0028 | 0.0026 | 0.0009 | 0.001 | 0.0037 | 0.0017 | 0.0055 | 0.0022 | 0.001 | 0.0006 | 0.0024 |
Nucleolus | 0.0055 | 0.0064 | 0.0089 | 0.0036 | 0.0043 | 0.0045 | 0.0044 | 0.0052 | 0.004 | 0.0067 | 0.002 | 0.0033 | 0.0048 | 0.0075 | 0.0104 | 0.0036 | 0.003 | 0.011 |
Nucleus | 0.0081 | 0.007 | 0.0066 | 0.0055 | 0.0139 | 0.0046 | 0.0026 | 0.0036 | 0.002 | 0.0019 | 0.0014 | 0.003 | 0.0022 | 0.0022 | 0.0038 | 0.0018 | 0.001 | 0.0037 |
Peroxisomes | 0.0111 | 0.0004 | 0.0008 | 0.0026 | 0.0174 | 0.0005 | 0.0009 | 0.0029 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0014 | 0.0037 | 0.0097 | 0.0022 | 0.0127 | 0.0006 |
Punctate Nuclear | 0.0095 | 0.0001 | 0.0043 | 0.0007 | 0.0008 | 0.0016 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 |
Vacuole | 0.5304 | 0.6981 | 0.6024 | 0.6653 | 0.5792 | 0.694 | 0.5583 | 0.6781 | 0.6197 | 0.7065 | 0.6706 | 0.6409 | 0.3707 | 0.5591 | 0.468 | 0.5301 | 0.4605 | 0.5839 |
Vacuole Periphery | 0.2303 | 0.2167 | 0.2023 | 0.1841 | 0.1631 | 0.2134 | 0.3032 | 0.2379 | 0.2419 | 0.1953 | 0.2434 | 0.2712 | 0.3182 | 0.2153 | 0.1758 | 0.2127 | 0.1627 | 0.276 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.6137 | 62.8906 | 27.6272 | 46.4053 | 113.6536 | 65.8132 | 73.6992 | 56.9093 | 41.8934 | 150.404 |
Translational Efficiency | 0.9323 | 0.9441 | 1.0187 | 0.3547 | 0.8715 | 1.1312 | 0.686 | 0.6435 | 0.7014 | 0.6468 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1402 | 1159 | 280 | 91 | 1308 | 1613 | 1084 | 1130 | 2710 | 2772 | 1364 | 1221 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 883.07 | 947.32 | 923.02 | 1075.99 | 868.04 | 1060.53 | 825.23 | 982.57 | 875.82 | 1013.20 | 845.30 | 989.53 |
Standard Deviation | 140.13 | 204.74 | 160.54 | 173.27 | 244.22 | 217.14 | 158.40 | 147.98 | 197.49 | 219.27 | 163.68 | 152.01 |
Intensity Change Log 2 | — | 0.101324 | 0.063834 | 0.285065 | — | 0.288952 | -0.072965 | 0.178799 | — | 0.197382 | -0.002358 | 0.233366 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001249 | 0.000090 | 0.002070 | 0.011486 | 0.000044 | 0.000032 | 0.005328 | 0.007578 | 0.000667 | 0.000056 | 0.004659 | 0.007869 |
Bud Neck | 0.022663 | 0.008059 | 0.020682 | 0.050560 | 0.006305 | 0.005480 | 0.037084 | 0.030426 | 0.014767 | 0.006558 | 0.033717 | 0.031926 |
Bud Site | 0.064025 | 0.026159 | 0.060409 | 0.113273* | 0.019069 | 0.005385 | 0.099428 | 0.111671* | 0.042327 | 0.014071 | 0.091418 | 0.111790* |
Cell Periphery | 0.001648 | 0.006667 | 0.000463 | 0.067570 | 0.026560 | 0.017526 | 0.001273 | 0.002074 | 0.013672 | 0.012986 | 0.001107 | 0.006956 |
Cytoplasm | 0.035091 | 0.016548 | 0.101197* | 0.069917 | 0.008139 | 0.000614 | 0.090820 | 0.062108 | 0.022082 | 0.007276 | 0.092950 | 0.062690 |
Cytoplasmic Foci | 0.042943 | 0.005733 | 0.089306 | 0.034737 | 0.001314 | 0.000538 | 0.105521 | 0.050605 | 0.022850 | 0.002710 | 0.102193 | 0.049423 |
Eisosomes | 0.000066 | 0.000043 | 0.000117 | 0.003170 | 0.000040 | 0.000138 | 0.000123 | 0.000090 | 0.000053 | 0.000099 | 0.000122 | 0.000319 |
Endoplasmic Reticulum | 0.004043 | 0.003741 | 0.005196 | 0.009125 | 0.004943 | 0.005871 | 0.004858 | 0.004121 | 0.004477 | 0.004980 | 0.004927 | 0.004494 |
Endosome | 0.324225* | 0.043732 | 0.134116 | 0.122468 | 0.013316 | 0.024696 | 0.283917* | 0.235138* | 0.174163 | 0.032655 | 0.253166* | 0.226740* |
Golgi | 0.026836 | 0.005407 | 0.007730 | 0.009844 | 0.000879 | 0.001416 | 0.013724 | 0.020543 | 0.014307 | 0.003085 | 0.012494 | 0.019746 |
Lipid Particles | 0.006527 | 0.000755 | 0.010005 | 0.007790 | 0.000428 | 0.000562 | 0.013687 | 0.005342 | 0.003583 | 0.000643 | 0.012931 | 0.005524 |
Mitochondria | 0.007548 | 0.003285 | 0.000288 | 0.007273 | 0.001210 | 0.004502 | 0.001366 | 0.002257 | 0.004489 | 0.003993 | 0.001145 | 0.002631 |
Mitotic Spindle | 0.006104 | 0.000341 | 0.002284 | 0.012819 | 0.000035 | 0.000118 | 0.003793 | 0.007561 | 0.003175 | 0.000211 | 0.003483 | 0.007953 |
None | 0.001381 | 0.000561 | 0.000486 | 0.000083 | 0.000773 | 0.000067 | 0.000593 | 0.000194 | 0.001088 | 0.000273 | 0.000571 | 0.000185 |
Nuclear Periphery | 0.000781 | 0.000097 | 0.000494 | 0.000153 | 0.000036 | 0.000270 | 0.000268 | 0.000172 | 0.000422 | 0.000198 | 0.000314 | 0.000171 |
Nuclear Periphery Foci | 0.002059 | 0.000281 | 0.010148 | 0.001527 | 0.000123 | 0.000359 | 0.004503 | 0.001291 | 0.001125 | 0.000326 | 0.005662 | 0.001309 |
Nucleolus | 0.002370 | 0.001228 | 0.001025 | 0.000298 | 0.000633 | 0.001143 | 0.001690 | 0.000697 | 0.001532 | 0.001179 | 0.001553 | 0.000668 |
Nucleus | 0.005261 | 0.002986 | 0.055912 | 0.005035 | 0.002110 | 0.001859 | 0.008493 | 0.004856 | 0.003740 | 0.002330 | 0.018227 | 0.004869 |
Peroxisomes | 0.010249 | 0.001796 | 0.016862 | 0.013680 | 0.000254 | 0.000508 | 0.015202 | 0.014690 | 0.005425 | 0.001047 | 0.015542 | 0.014615 |
Vacuole | 0.398749* | 0.779432* | 0.475147* | 0.454130* | 0.858947* | 0.871771* | 0.301517* | 0.426295* | 0.620867* | 0.833163* | 0.337159* | 0.428370* |
Vacuole Periphery | 0.036184 | 0.093059 | 0.006064 | 0.005062 | 0.054843 | 0.057142 | 0.006813 | 0.012292 | 0.045189 | 0.072159 | 0.006659 | 0.011753 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.83 | -1.68 | -2.19 | -2.49 | -1.95 | 0.82 | -5.17 | -4.44 | -4.45 | -1.07 | 3.89 | -4.81 | -4.42 | -4.84 | -1.66 |
Bud Neck | 8.20 | 1.24 | -2.16 | -3.79 | -2.51 | 0.94 | -12.24 | -11.04 | -11.67 | 2.03 | 8.08 | -8.41 | -7.39 | -11.90 | 0.65 |
Bud Site | 8.76 | 1.09 | -2.34 | -4.50 | -2.67 | 6.45 | -13.94 | -15.24 | -18.43 | -1.80 | 11.72 | -9.38 | -11.72 | -17.47 | -3.16 |
Cell Periphery | -6.85 | 5.10 | -2.75 | -2.48 | -2.81 | 4.16 | 14.05 | 13.47 | 11.86 | -1.55 | 0.57 | 13.56 | 4.47 | 4.21 | -3.14 |
Cytoplasm | 5.31 | -6.74 | -2.75 | -4.06 | 1.64 | 5.59 | -15.45 | -12.88 | -15.07 | 3.86 | 7.99 | -14.60 | -9.90 | -13.88 | 4.59 |
Cytoplasmic Foci | 15.14 | -5.87 | 1.19 | -2.42 | 4.46 | 2.18 | -23.25 | -16.37 | -16.72 | 10.10 | 15.25 | -19.45 | -8.37 | -16.01 | 10.82 |
Eisosomes | 2.75 | -3.37 | -2.07 | -2.09 | -2.02 | -8.59 | -5.72 | -5.20 | 3.42 | 2.07 | -5.81 | -5.69 | -2.40 | -1.91 | -1.61 |
Endoplasmic Reticulum | 0.35 | -1.71 | -1.43 | -1.53 | -0.82 | -1.74 | -0.00 | 1.18 | 2.33 | 1.04 | -1.05 | -1.15 | 0.11 | 0.81 | 0.93 |
Endosome | 33.38* | 15.83 | 10.05 | -2.60 | 0.88 | -5.39 | -31.04* | -27.17 | -25.50 | 3.94 | 26.50* | -7.97 | -5.19 | -24.45 | 2.20 |
Golgi | 10.30 | 8.11 | 5.89 | -0.89 | -0.41 | -2.16 | -10.70 | -11.38 | -11.01 | -3.36 | 10.34 | 1.70 | -2.62 | -9.96 | -3.94 |
Lipid Particles | 4.68 | -1.64 | -0.08 | -1.91 | 0.95 | -0.63 | -9.37 | -4.46 | -4.34 | 4.78 | 4.55 | -6.97 | -1.46 | -4.60 | 4.89 |
Mitochondria | 3.13 | 6.74 | 0.34 | -0.49 | -1.09 | -3.06 | -0.37 | -2.04 | 2.17 | -2.38 | 0.52 | 5.51 | 2.62 | 1.82 | -2.72 |
Mitotic Spindle | 4.79 | 2.14 | -0.53 | -1.19 | -0.90 | -1.37 | -4.88 | -4.83 | -4.78 | -1.95 | 4.74 | -0.88 | -2.83 | -4.81 | -2.30 |
None | 1.02 | 1.04 | 1.29 | 0.61 | 0.70 | 2.97 | 0.53 | 2.33 | -2.50 | 1.65 | 1.92 | 1.10 | 2.10 | 0.50 | 1.92 |
Nuclear Periphery | 3.00 | 0.74 | 2.24 | -3.16 | 4.18 | -3.67 | -9.04 | -5.63 | 1.17 | 2.89 | 1.84 | 0.50 | 1.91 | 0.06 | 4.68 |
Nuclear Periphery Foci | 4.24 | -3.77 | 1.00 | -5.58 | 4.04 | -5.61 | -7.17 | -5.99 | -4.81 | 5.10 | 3.76 | -6.44 | -0.26 | -5.54 | 6.50 |
Nucleolus | 2.23 | 2.58 | 3.70 | 3.61 | 3.27 | -2.00 | -3.42 | -0.44 | 1.30 | 2.71 | 1.53 | 0.43 | 2.68 | 1.86 | 2.84 |
Nucleus | 2.67 | -6.27 | -1.30 | -1.64 | 4.03 | 0.35 | -5.88 | -3.18 | -3.41 | 3.08 | 2.61 | -7.68 | -2.10 | -3.52 | 6.12 |
Peroxisomes | 6.65 | -1.41 | -0.21 | -2.34 | 0.81 | -1.29 | -12.04 | -8.85 | -8.64 | 0.22 | 6.90 | -7.18 | -5.41 | -8.50 | 0.43 |
Vacuole | -34.42* | -3.95 | -2.68 | 7.40 | -0.53 | -1.94 | 53.78* | 37.78* | 40.44* | -8.89 | -27.30 | 26.36 | 15.85 | 37.82 | -7.05 |
Vacuole Periphery | -13.52 | 14.46 | 14.44 | 22.42 | 0.74 | -0.56 | 15.32 | 13.17 | 15.98 | -5.13 | -9.45 | 20.95 | 16.76 | 25.20 | -5.22 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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