Standard name
Human Ortholog
Description Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0 0.06 – 0 0 0 0 0 0 0 0.15 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0.07 0.13 0 0.09 – 0 0 0.07 0 0.1 0.09 0.08 0.11 0.07 0.08 0 0.07 0 0.06 0.08 0.06
Golgi 0 0.06 0.2 0.09 0.08 0 0 – 0 0 0 0 0.16 0.21 0.31 0.36 0.48 0.36 0.05 0.08 0.05 0.07 0.08 0.09
Mitochondria 0.86 0.89 0.77 0.82 0.64 0.9 0.69 – 0.9 0.88 0.9 0.93 0.8 0.76 0.65 0.14 0.1 0.07 0.78 0.74 0.81 0.71 0.65 0.64
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0 0 0 – 0 0 0 0 0.06 0.05 0.07 0.05 0.07 0.07 0.05 0 0.06 0.07 0.06 0.07
SpindlePole 0 0 0.06 0 0.07 0 0.06 – 0 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.17 0.09 0.08 0.16 0.09 0.17 – 0.09 0.05 0.1 0.05 0.12 0.1 0.11 0.19 0.2 0.22 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 9 3 3 1 0 – 0 0 0 0 1 0 2 4 2 4 1 10 0 3 7 9
Bud 3 7 3 10 6 11 5 – 13 22 18 10 2 3 1 2 2 3 0 2 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 1 0 0 3 2
Bud Site 0 1 1 2 0 3 1 – 2 2 3 4 0 0 1 0 1 0 – – – – – –
Cell Periphery 3 31 13 6 3 0 1 – 3 6 9 2 3 5 6 8 14 13 1 1 1 0 0 0
Cytoplasm 5 7 6 4 21 4 17 – 12 9 6 1 11 10 8 15 12 11 0 1 0 0 0 1
Endoplasmic Reticulum 3 10 4 0 3 4 1 – 4 2 0 3 2 5 3 4 1 3 0 2 0 0 0 5
Endosome 11 17 16 14 32 3 27 – 6 9 35 17 30 30 24 11 9 10 15 49 12 12 29 22
Golgi 13 38 55 18 21 1 5 – 1 2 0 2 49 71 90 37 64 43 15 59 15 13 28 32
Mitochondria 286 603 208 158 162 259 201 – 380 360 466 482 251 251 189 14 14 8 263 543 256 142 239 229
Nucleus 3 5 1 1 0 11 11 – 7 10 6 7 0 0 2 2 2 4 0 1 0 1 0 1
Nuclear Periphery 11 11 5 0 6 7 10 – 23 5 8 10 7 8 6 0 1 1 6 5 0 1 4 5
Nucleolus 14 10 1 1 4 10 5 – 15 8 17 7 13 5 16 0 0 3 1 1 0 0 3 1
Peroxisomes 4 13 3 11 4 0 4 – 4 7 11 6 19 15 21 5 9 9 16 17 18 13 22 25
SpindlePole 7 6 16 8 18 10 18 – 15 35 18 23 5 3 8 5 4 4 2 2 0 0 3 1
Vac/Vac Membrane 53 117 23 15 41 26 50 – 36 21 51 25 36 33 33 19 27 27 10 31 6 7 16 15
Unique Cell Count 331 681 270 192 255 289 292 422 411 517 521 313 331 290 102 134 121 339 733 316 201 366 359
Labelled Cell Count 416 880 364 251 324 350 356 521 499 648 599 429 439 410 126 162 143 339 733 316 201 366 359


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.5 6.7 6.6 7.3 4.8 5.9 – 5.3 5.1 5.3 5.3 7.9 8.0 8.0 8.7 9.8 8.7 6.6 6.9 7.0
Std Deviation (1e-4) 1.0 0.9 0.9 1.0 2.0 1.1 1.4 – 1.3 0.9 1.1 0.9 1.3 1.4 1.2 1.8 2.2 1.6 1.5 1.4 1.5
Intensity Change (Log2) – – – -0.03 0.11 -0.5 -0.19 – -0.34 -0.39 -0.35 -0.35 0.24 0.25 0.24 0.36 0.54 0.36 -0.02 0.03 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.7 0 0 – 0 0 0 0 0 0 -2.3 0 0 0
Bud 2.6 0 0 0 – 1.7 2.9 2.0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -2.4 0 -3.4 – -3.5 -2.6 -2.5 -4.3 -2.8 -2.4 -1.8 1.1 2.1 2.2
Cytoplasm 0 3.1 0 2.2 – 0.5 0 0 0 0.9 0.6 0.4 4.7 3.1 3.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0.6 2.6 0 1.5 – -3.3 -2.5 0.5 -1.8 1.6 1.4 1.1 1.6 0.3 0.9
Golgi -3.2 -3.9 0 -7.2 – -9.5 -9.2 -10.6 -10.3 -1.5 0.3 2.9 3.2 5.7 3.2
Mitochondria 1.4 -3.4 0 -2.2 – 4.7 3.6 5.0 6.2 0.9 -0.3 -3.1 -11.1 -12.7 -12.9
Nucleus 0 0 2.8 2.8 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.4 0 1.2 – 2.3 0 0 0.1 0.3 0.5 0.2 0 0 0
Nucleolus 0 0 0 0 – 2.7 0 2.6 0 3.0 0 3.5 0 0 0
Peroxisomes 2.9 0 0 0 – 0 0 0 0 3.1 2.4 3.6 0 0 0
SpindlePole -0.8 0.5 0 0.1 – -1.5 1.3 -1.6 -0.9 -2.8 -3.5 -1.8 -0.4 -1.3 -1.1
Vacuole -0.3 2.6 0.2 3.0 – 0 -1.8 0.6 -2.1 1.2 0.6 1.1 2.7 3.3 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6653 3.306 3.1733 2.7569 2.8222 2.9914 3.7998 4.6744 4.2043 3.4393 4.4111 4.2801 8.2049 8.1915 7.9475 8.0623 7.6847 8.2116
Actin 0.0401 0.0035 0.0115 0.0007 0.001 0.0005 0.0414 0.0132 0.0388 0.0014 0.002 0.0106 0.01 0.0064 0.006 0.0349 0.0054 0.0017
Bud 0.0004 0.0001 0.0007 0.0001 0.0001 0.0001 0.0009 0.0008 0.0006 0.0002 0.0006 0.0002 0.0034 0.0008 0.0002 0.0012 0.0001 0.0001
Bud Neck 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0003 0.0003 0.0003 0.0002 0.0005 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.001 0.0002 0.0022 0.0001 0.0002 0.0005 0.0016 0.0009 0.0011 0.0005 0.0011 0.0004 0.0085 0.0011 0.0004 0.0022 0.0004 0.0004
Bud Site 0.001 0.0004 0.0027 0.0001 0.0001 0.0001 0.0033 0.003 0.0029 0.0003 0.002 0.0013 0.0006 0.0044 0.0003 0.0009 0.0003 0.0001
Cell Periphery 0.0004 0.0001 0.0005 0.0001 0.0001 0.0001 0.0004 0.0002 0.0011 0.0001 0.0002 0.0002 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0007 0.0001 0.0005 0.0002 0 0.0001 0.0007 0.0003 0.0001 0.0001 0.0001 0.0002 0.0003 0.0003 0.0001 0.0002 0.0001 0.0001
Cytoplasmic Foci 0.0166 0.004 0.0064 0.0087 0.0054 0.0017 0.0323 0.0109 0.0049 0.0076 0.0119 0.0071 0.004 0.0083 0.0053 0.002 0.0033 0.0027
Eisosomes 0.0007 0.0001 0.0003 0.0001 0.0001 0.0001 0.0006 0.0003 0.0002 0.0001 0.0001 0.0002 0.0004 0.0002 0.0001 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0024 0.0002 0.0008 0.0002 0 0.0001 0.0009 0.0003 0.0001 0 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001
Endosome 0.028 0.0204 0.0227 0.0488 0.0136 0.0105 0.0319 0.0177 0.009 0.012 0.0058 0.029 0.0233 0.019 0.0103 0.0159 0.0057 0.007
Golgi 0.0382 0.0375 0.0282 0.0314 0.0212 0.0339 0.0293 0.0405 0.0117 0.023 0.0081 0.0506 0.0196 0.033 0.02 0.0328 0.0301 0.0191
Lipid Particles 0.0487 0.012 0.0226 0.0302 0.0412 0.0042 0.0642 0.0232 0.0105 0.0074 0.0328 0.0093 0.0212 0.0243 0.0086 0.0022 0.0042 0.0047
Mitochondria 0.7523 0.8923 0.8673 0.8621 0.8978 0.9386 0.7141 0.843 0.8748 0.9295 0.8634 0.8509 0.8817 0.8614 0.9243 0.903 0.9308 0.9447
None 0.0012 0 0.0009 0 0.0001 0 0.001 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0 0.0001 0 0.0001
Nuclear Periphery 0.0087 0.0002 0.0018 0.0001 0.0001 0.0001 0.0025 0.0014 0 0 0.0001 0.0001 0.0014 0.0004 0 0 0.0001 0.0005
Nucleolus 0.0016 0.0002 0.0008 0.0002 0.0002 0.0001 0.0023 0.0036 0.0005 0.0001 0.0017 0.0003 0.0022 0.0003 0.0001 0 0 0.0001
Nucleus 0.0017 0.0001 0.0009 0.0001 0.0001 0.0001 0.002 0.0009 0.0002 0.0001 0.0005 0.0002 0.0002 0.0002 0.0001 0 0 0.0002
Peroxisomes 0.0424 0.0186 0.0134 0.0103 0.0139 0.0025 0.0543 0.0326 0.031 0.0151 0.0661 0.0312 0.0093 0.0342 0.0191 0.0019 0.0179 0.0135
Punctate Nuclear 0.0028 0 0.0035 0.0003 0.0003 0 0.0048 0.001 0.0001 0 0.0004 0.0002 0.0001 0.0001 0 0 0 0
Vacuole 0.0043 0.0044 0.0041 0.0022 0.0014 0.0021 0.0049 0.0021 0.0092 0.001 0.0014 0.0039 0.0046 0.0016 0.0015 0.0006 0.0002 0.0021
Vacuole Periphery 0.0063 0.0054 0.008 0.0042 0.0031 0.0047 0.0062 0.0034 0.0028 0.0012 0.0014 0.0033 0.0085 0.0033 0.0031 0.0015 0.0007 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.7151 30.1393 28.4641 33.3879 26.824 24.5065 37.0534 35.5261 36.6007 44.2413
Translational Efficiency 1.13 1.139 1.1769 0.8264 1.2506 1.6742 0.8817 1.0411 0.8821 0.8782

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Tim21

Tim21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tim21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available