Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4 | 7 | 1 | 3 | 2 | 0 | 0 | 33 | 10 | 16 | 16 | 26 |
Bud | 2 | 7 | 3 | 1 | 3 | 3 | 10 | 3 | – | 5 | 6 | 17 | 4 | 0 | 0 | 2 | 3 | 0 | 1 | 10 | 0 | 5 | 6 | 21 |
Bud Neck | 0 | 4 | 4 | 0 | 1 | 5 | 1 | 0 | – | 2 | 1 | 0 | 8 | 10 | 3 | 3 | 4 | 1 | 0 | 12 | 1 | 12 | 17 | 20 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 10 | 6 | 0 | 0 | 1 | 0 | 2 | – | 5 | 1 | 2 | 5 | 6 | 3 | 6 | 4 | 7 | 0 | 8 | 3 | 2 | 5 | 6 |
Cytoplasm | 23 | 369 | 104 | 61 | 114 | 132 | 102 | 135 | – | 59 | 66 | 94 | 477 | 620 | 642 | 198 | 150 | 87 | 8 | 150 | 38 | 45 | 37 | 65 |
Endoplasmic Reticulum | 1 | 9 | 6 | 1 | 0 | 1 | 4 | 0 | – | 4 | 3 | 2 | 2 | 6 | 5 | 23 | 17 | 11 | 0 | 11 | 4 | 10 | 6 | 10 |
Endosome | 0 | 23 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 16 | 10 | 9 | 14 | 24 | 6 | 2 | 60 | 7 | 31 | 23 | 24 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 2 | 0 | 4 | 0 | 1 | 5 | 5 |
Mitochondria | 24 | 74 | 63 | 31 | 59 | 141 | 210 | 84 | – | 224 | 181 | 249 | 2 | 3 | 0 | 20 | 15 | 9 | 38 | 297 | 148 | 346 | 315 | 390 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | – | 0 | 2 | 4 | 0 | 3 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 1 | 0 | 2 | 4 | 0 | 0 | 2 | – | 0 | 0 | 0 | 4 | 1 | 1 | 3 | 8 | 3 | 0 | 1 | 2 | 3 | 3 | 4 |
SpindlePole | 0 | 9 | 9 | 3 | 6 | 6 | 1 | 9 | – | 10 | 3 | 14 | 8 | 1 | 3 | 15 | 11 | 4 | 0 | 17 | 5 | 22 | 18 | 18 |
Vac/Vac Membrane | 2 | 34 | 3 | 0 | 7 | 4 | 1 | 2 | – | 4 | 1 | 1 | 26 | 11 | 12 | 8 | 8 | 12 | 1 | 49 | 9 | 53 | 44 | 64 |
Unique Cell Count | 44 | 498 | 179 | 92 | 175 | 240 | 267 | 203 | 267 | 214 | 308 | 538 | 661 | 666 | 264 | 217 | 123 | 59 | 689 | 241 | 571 | 514 | 675 | |
Labelled Cell Count | 52 | 545 | 200 | 101 | 196 | 295 | 332 | 241 | 317 | 267 | 387 | 556 | 680 | 682 | 300 | 250 | 144 | 59 | 689 | 241 | 571 | 514 | 675 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 4.2 | 3.5 | 3.8 | 3.3 | 3.1 | 2.6 | 3.9 | – | 2.6 | 2.6 | 2.6 | 5.3 | 5.2 | 5.2 | 5.5 | 5.4 | 5.9 | 5.6 | 5.7 | 5.9 |
Std Deviation (1e-4) | 2.4 | 0.9 | 0.6 | 1.5 | 1.2 | 0.9 | 1.2 | 11.6 | – | 0.3 | 0.5 | 0.4 | 0.8 | 0.9 | 1.0 | 1.3 | 1.3 | 1.9 | 1.1 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.14 | -0.06 | -0.14 | -0.39 | 0.18 | – | -0.43 | -0.43 | -0.41 | 0.62 | 0.57 | 0.58 | 0.66 | 0.63 | 0.77 | 0.68 | 0.71 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2944 | 0.343 | 0.2749 | -0.3705 | -0.1397 | 0.3272 | 2.992 | 2.8328 | 3.3177 | 2.8423 | 2.7971 | 3.3067 | 0.8155 | 1.1334 | 1.2444 | 0.8255 | 0.9296 | 1.1071 |
Actin | 0.0024 | 0.004 | 0.0063 | 0.0038 | 0.0029 | 0.0039 | 0.0294 | 0.0099 | 0.0207 | 0.007 | 0.0232 | 0.0093 | 0.002 | 0.0057 | 0.0045 | 0.0041 | 0.0058 | 0.0024 |
Bud | 0.0016 | 0.0077 | 0.0026 | 0.0032 | 0.0086 | 0.0017 | 0.0024 | 0.0047 | 0.0061 | 0.0056 | 0.0069 | 0.0038 | 0.0015 | 0.0033 | 0.0046 | 0.0034 | 0.0026 | 0.0015 |
Bud Neck | 0.0069 | 0.0019 | 0.0024 | 0.0247 | 0.005 | 0.0096 | 0.0063 | 0.0039 | 0.0043 | 0.0042 | 0.0148 | 0.0135 | 0.0026 | 0.0019 | 0.0045 | 0.0013 | 0.002 | 0.008 |
Bud Periphery | 0.004 | 0.008 | 0.0109 | 0.01 | 0.0285 | 0.0025 | 0.0059 | 0.008 | 0.0135 | 0.0174 | 0.0111 | 0.0072 | 0.0048 | 0.0057 | 0.0108 | 0.0097 | 0.0078 | 0.0043 |
Bud Site | 0.0079 | 0.0327 | 0.0101 | 0.0096 | 0.0201 | 0.0023 | 0.0098 | 0.0571 | 0.016 | 0.0048 | 0.0163 | 0.0032 | 0.0092 | 0.0278 | 0.0207 | 0.0028 | 0.0025 | 0.002 |
Cell Periphery | 0.0017 | 0.0025 | 0.0017 | 0.0014 | 0.0011 | 0.0007 | 0.0024 | 0.0018 | 0.0037 | 0.0015 | 0.001 | 0.0013 | 0.0013 | 0.0018 | 0.0024 | 0.0007 | 0.0006 | 0.0008 |
Cytoplasm | 0.0645 | 0.1198 | 0.0812 | 0.0651 | 0.0551 | 0.115 | 0.0873 | 0.1341 | 0.0807 | 0.0809 | 0.1079 | 0.1267 | 0.1132 | 0.1442 | 0.1362 | 0.0901 | 0.0963 | 0.1208 |
Cytoplasmic Foci | 0.1327 | 0.1145 | 0.1398 | 0.1234 | 0.1452 | 0.1439 | 0.1353 | 0.1809 | 0.1264 | 0.1517 | 0.1929 | 0.1743 | 0.1234 | 0.1231 | 0.1215 | 0.1273 | 0.1823 | 0.159 |
Eisosomes | 0.0011 | 0.0009 | 0.0015 | 0.0008 | 0.0007 | 0.0003 | 0.0013 | 0.0005 | 0.0015 | 0.0009 | 0.0004 | 0.0004 | 0.0006 | 0.0006 | 0.0007 | 0.0003 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.0036 | 0.0096 | 0.0064 | 0.0021 | 0.0043 | 0.0043 | 0.0067 | 0.003 | 0.0039 | 0.0063 | 0.0035 | 0.0052 | 0.0095 | 0.008 | 0.0069 | 0.0063 | 0.0028 | 0.006 |
Endosome | 0.1456 | 0.1655 | 0.1462 | 0.1099 | 0.1525 | 0.1795 | 0.1643 | 0.1769 | 0.1399 | 0.1752 | 0.2333 | 0.1498 | 0.1859 | 0.219 | 0.163 | 0.1983 | 0.2255 | 0.1922 |
Golgi | 0.0293 | 0.0283 | 0.0289 | 0.0362 | 0.0276 | 0.0347 | 0.0526 | 0.0536 | 0.0428 | 0.036 | 0.0521 | 0.0417 | 0.0343 | 0.0431 | 0.0354 | 0.0416 | 0.0425 | 0.037 |
Lipid Particles | 0.0513 | 0.0223 | 0.0206 | 0.0091 | 0.0314 | 0.0307 | 0.0353 | 0.0065 | 0.0099 | 0.0162 | 0.0147 | 0.0186 | 0.0366 | 0.0137 | 0.0157 | 0.0301 | 0.0189 | 0.019 |
Mitochondria | 0.1689 | 0.1643 | 0.1837 | 0.2449 | 0.1504 | 0.1634 | 0.1576 | 0.1631 | 0.2343 | 0.251 | 0.1153 | 0.1546 | 0.1731 | 0.1903 | 0.2033 | 0.2328 | 0.2032 | 0.1985 |
None | 0.2992 | 0.24 | 0.2794 | 0.2607 | 0.2896 | 0.2255 | 0.2332 | 0.1439 | 0.2279 | 0.1264 | 0.1369 | 0.208 | 0.2401 | 0.1552 | 0.1974 | 0.1786 | 0.1263 | 0.176 |
Nuclear Periphery | 0.0015 | 0.0048 | 0.0032 | 0.0013 | 0.0012 | 0.0018 | 0.0054 | 0.0007 | 0.0038 | 0.0028 | 0.0012 | 0.0018 | 0.0049 | 0.0032 | 0.0027 | 0.002 | 0.0017 | 0.0019 |
Nucleolus | 0.0052 | 0.0029 | 0.0036 | 0.002 | 0.0021 | 0.0012 | 0.0024 | 0.002 | 0.0015 | 0.0013 | 0.0013 | 0.0013 | 0.0019 | 0.002 | 0.0028 | 0.0023 | 0.0011 | 0.0016 |
Nucleus | 0.0033 | 0.0056 | 0.0044 | 0.0032 | 0.0022 | 0.0024 | 0.0038 | 0.0024 | 0.0032 | 0.0025 | 0.0025 | 0.0029 | 0.0033 | 0.0043 | 0.0049 | 0.0043 | 0.0017 | 0.003 |
Peroxisomes | 0.0419 | 0.0334 | 0.0402 | 0.0699 | 0.0516 | 0.0536 | 0.0332 | 0.0279 | 0.0371 | 0.0777 | 0.0391 | 0.0572 | 0.026 | 0.0152 | 0.0264 | 0.023 | 0.0513 | 0.0447 |
Punctate Nuclear | 0.008 | 0.0041 | 0.0083 | 0.003 | 0.0025 | 0.0034 | 0.0075 | 0.0032 | 0.0043 | 0.0018 | 0.0016 | 0.0028 | 0.0026 | 0.0028 | 0.0041 | 0.0022 | 0.0013 | 0.0039 |
Vacuole | 0.0157 | 0.0212 | 0.0136 | 0.0116 | 0.0128 | 0.0145 | 0.0139 | 0.0125 | 0.0143 | 0.0196 | 0.0198 | 0.012 | 0.018 | 0.0232 | 0.0254 | 0.0312 | 0.0167 | 0.0127 |
Vacuole Periphery | 0.0035 | 0.0061 | 0.0047 | 0.0041 | 0.0046 | 0.0049 | 0.004 | 0.0034 | 0.0041 | 0.0091 | 0.0041 | 0.0046 | 0.005 | 0.0059 | 0.0062 | 0.0075 | 0.0069 | 0.0044 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.2543 | 10.4378 | 2.3236 | 7.2906 | 1.5139 | 4.6679 | 8.1791 | 4.9861 | 8.7377 | 8.0079 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|