Standard name | |
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Human Ortholog | |
Description | Regulator of the Fps1p glycerol channel; under nonstress conditions, binds to Fps1p to positively regulate glycerol transport; under osmotic stress, multiple phosphorylation by Hog1p causes Ask10p to dissociate from Fps1p; forms homodimers and heterodimerizes with paralog Rgc1p; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; associates with RNA polymerase II holoenzyme |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 5 | 7 | 29 | 74 | 38 | 51 | 50 | 30 | – | 7 | 6 | 16 | 34 | 33 | 4 | 2 | 6 | 33 | 15 | 20 | 10 | 22 | 33 |
Bud | 9 | 7 | 10 | 10 | 38 | 40 | 28 | 42 | 27 | – | 24 | 33 | 15 | 26 | 38 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 4 | 12 | 17 | 18 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 75 | 57 | 32 | 91 | 133 | 95 | 132 | 91 | 102 | – | 37 | 84 | 149 | 205 | 230 | 24 | 13 | 41 | 109 | 58 | 51 | 42 | 92 | 141 |
Cytoplasm | 145 | 84 | 67 | 98 | 146 | 192 | 301 | 360 | 261 | – | 152 | 286 | 167 | 274 | 370 | 37 | 30 | 59 | 49 | 41 | 23 | 57 | 147 | 155 |
Endoplasmic Reticulum | 6 | 2 | 1 | 0 | 3 | 4 | 5 | 10 | 5 | – | 6 | 7 | 4 | 2 | 4 | 2 | 2 | 0 | 22 | 2 | 5 | 4 | 15 | 26 |
Endosome | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 2 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 1 | 6 | 4 | 16 | 31 | 75 | 137 | 149 | 161 | – | 116 | 245 | 1 | 2 | 9 | 1 | 2 | 0 | 15 | 1 | 7 | 1 | 4 | 3 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | – | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 4 | 1 | 2 | 1 | 3 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 10 | 3 | 1 | 2 | 3 | 4 |
Unique Cell Count | 216 | 123 | 94 | 158 | 277 | 294 | 478 | 544 | 427 | 254 | 484 | 242 | 370 | 472 | 51 | 40 | 96 | 266 | 136 | 118 | 137 | 313 | 392 | |
Labelled Cell Count | 267 | 163 | 121 | 245 | 432 | 448 | 661 | 712 | 594 | 346 | 663 | 352 | 544 | 685 | 68 | 51 | 117 | 266 | 136 | 118 | 137 | 313 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 7.4 | 5.3 | 5.7 | 6.1 | 5.4 | 5.2 | 5.2 | 4.4 | – | 4.1 | 4.0 | 8.9 | 8.3 | 7.1 | 11.0 | 11.0 | 13.0 | 8.2 | 8.4 | 9.1 |
Std Deviation (1e-4) | 1.6 | 1.4 | 1.0 | 1.0 | 1.3 | 1.0 | 1.1 | 1.2 | 0.8 | – | 0.6 | 0.8 | 1.7 | 1.4 | 1.2 | 2.4 | 2.7 | 2.5 | 1.5 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.2 | 0.02 | -0.02 | -0.03 | -0.27 | – | -0.36 | -0.43 | 0.75 | 0.64 | 0.41 | 1.05 | 1.04 | 1.29 | 0.62 | 0.66 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.4 | 3.9 | 1.4 | 0.9 | 0.5 | 0 | – | 0 | 0 | -0.3 | 0.5 | -0.2 | 0 | 0 | -0.3 |
Bud | -1.2 | 0.8 | 0.7 | -1.7 | -1.0 | 0 | – | 0 | 0 | -1.4 | -1.2 | -0.8 | 0 | 0 | -2.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.6 | 2.4 | -0.3 | -1.3 | -3.9 | 0 | – | 0 | 0 | 4.5 | 3.7 | 2.6 | 1.5 | -0.2 | 1.2 |
Cytoplasm | -1.5 | -3.1 | -1.1 | -1.5 | -1.0 | -1.8 | – | -2.0 | -2.2 | -0.4 | 0.5 | 1.5 | 0.2 | 0.4 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.7 | 2.0 | 4.5 | 5.0 | 4.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8091 | 8.045 | 6.9881 | 7.1549 | 5.9715 | 7.167 | 4.0138 | 5.9311 | 5.5743 | 5.1739 | 4.3526 | 5.62 | 1.6736 | 3.2014 | 2.516 | 3.0922 | 1.9689 | 2.4168 |
Actin | 0.0029 | 0.0166 | 0.0072 | 0.002 | 0.0067 | 0.0029 | 0.0639 | 0.0088 | 0.0127 | 0.0087 | 0.0674 | 0.0262 | 0.0426 | 0.0075 | 0.0236 | 0.0155 | 0.006 | 0.0188 |
Bud | 0.0007 | 0.0009 | 0.001 | 0.0006 | 0.0007 | 0.0002 | 0.0019 | 0.0005 | 0.0006 | 0.0008 | 0.002 | 0.0007 | 0.0002 | 0.0003 | 0.005 | 0.0026 | 0.0002 | 0.0003 |
Bud Neck | 0.001 | 0.0016 | 0.0021 | 0.0024 | 0.0042 | 0.0088 | 0.0051 | 0.0017 | 0.0027 | 0.0103 | 0.0083 | 0.0113 | 0.0016 | 0.0014 | 0.0086 | 0.0155 | 0.0054 | 0.0125 |
Bud Periphery | 0.0023 | 0.002 | 0.0039 | 0.0026 | 0.0049 | 0.0011 | 0.0033 | 0.0007 | 0.0009 | 0.0017 | 0.003 | 0.0012 | 0.0005 | 0.001 | 0.0067 | 0.0119 | 0.0006 | 0.002 |
Bud Site | 0.0025 | 0.0298 | 0.0079 | 0.0015 | 0.0022 | 0.0007 | 0.0168 | 0.0252 | 0.0107 | 0.006 | 0.0096 | 0.0021 | 0.0082 | 0.0307 | 0.027 | 0.0062 | 0.001 | 0.0004 |
Cell Periphery | 0.0279 | 0.034 | 0.0409 | 0.0256 | 0.0297 | 0.0135 | 0.0096 | 0.0108 | 0.0087 | 0.0034 | 0.0056 | 0.0027 | 0.0254 | 0.0289 | 0.0237 | 0.0427 | 0.0167 | 0.0195 |
Cytoplasm | 0.8839 | 0.8928 | 0.9099 | 0.9276 | 0.934 | 0.9446 | 0.6813 | 0.9226 | 0.8934 | 0.8978 | 0.6258 | 0.9235 | 0.8079 | 0.8679 | 0.8065 | 0.8522 | 0.9318 | 0.9228 |
Cytoplasmic Foci | 0.0019 | 0.0024 | 0.0018 | 0.0025 | 0.0018 | 0.0015 | 0.0307 | 0.0019 | 0.0142 | 0.0125 | 0.0312 | 0.004 | 0.0063 | 0.003 | 0.0125 | 0.0032 | 0.0012 | 0.0015 |
Eisosomes | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0008 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0261 | 0.009 | 0.0113 | 0.0221 | 0.0053 | 0.0096 | 0.0159 | 0.0021 | 0.0042 | 0.0052 | 0.0051 | 0.0025 | 0.0069 | 0.0034 | 0.0029 | 0.0026 | 0.0017 | 0.002 |
Endosome | 0.0006 | 0.0012 | 0.0007 | 0.0016 | 0.0005 | 0.0008 | 0.0508 | 0.0003 | 0.0082 | 0.0074 | 0.0686 | 0.0029 | 0.0028 | 0.0005 | 0.0046 | 0.0007 | 0.0002 | 0.0002 |
Golgi | 0.0006 | 0.0021 | 0.0009 | 0.0005 | 0.0006 | 0.0005 | 0.0199 | 0.0005 | 0.0029 | 0.0092 | 0.0664 | 0.0022 | 0.0024 | 0.0009 | 0.0031 | 0.0014 | 0.0002 | 0.0004 |
Lipid Particles | 0.0011 | 0.0018 | 0.0023 | 0.0007 | 0.0004 | 0.0006 | 0.0191 | 0.0004 | 0.0037 | 0.0147 | 0.0226 | 0.0027 | 0.0015 | 0.0014 | 0.0072 | 0.0006 | 0.0001 | 0.0002 |
Mitochondria | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0127 | 0.0001 | 0.0011 | 0.0033 | 0.0455 | 0.0004 | 0.0004 | 0.0001 | 0.0026 | 0.0002 | 0 | 0 |
None | 0.0454 | 0.0026 | 0.0061 | 0.0051 | 0.0055 | 0.0128 | 0.0127 | 0.0233 | 0.0161 | 0.0081 | 0.0037 | 0.0135 | 0.0758 | 0.0497 | 0.0556 | 0.0421 | 0.0341 | 0.0186 |
Nuclear Periphery | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0261 | 0.0001 | 0.0025 | 0.0007 | 0.0131 | 0.0009 | 0.0038 | 0.0002 | 0.0019 | 0.0002 | 0.0001 | 0.0001 |
Nucleolus | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 |
Nucleus | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.0005 | 0.0056 | 0.0002 | 0.0022 | 0.0005 | 0.0013 | 0.0006 | 0.008 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0.0001 |
Peroxisomes | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0085 | 0.0001 | 0.0099 | 0.0075 | 0.0107 | 0.0011 | 0.0009 | 0 | 0.0038 | 0.0002 | 0 | 0 |
Punctate Nuclear | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0071 | 0.0001 | 0.0025 | 0.0007 | 0.0032 | 0.0007 | 0.0027 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0 |
Vacuole | 0.0017 | 0.0012 | 0.0025 | 0.0038 | 0.0023 | 0.0011 | 0.005 | 0.0004 | 0.002 | 0.0011 | 0.0036 | 0.0004 | 0.0009 | 0.0018 | 0.002 | 0.0013 | 0.0003 | 0.0004 |
Vacuole Periphery | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0026 | 0 | 0.0004 | 0.0002 | 0.0023 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.1638 | 29.812 | 14.2515 | 22.6725 | 16.2144 | 16.6653 | 29.998 | 24.2568 | 20.5245 | 27.8907 |
Translational Efficiency | 0.6909 | 0.5836 | 0.9242 | 0.5132 | 0.7617 | 1.0027 | 0.5537 | 0.6153 | 0.7177 | 0.6071 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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