Standard name
Human Ortholog
Description Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0.07 0 0 0.06 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0.06 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.08 0.07 0.09 0.05 0.06 0 0.05 0.08 0 0.11 0.12 0.15 0.15 0 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0.08 0.12 0 0 0 0 0 0
Endosome 0.06 0.07 0.11 0.26 0.27 0 0.19 0.1 0.06 0.05 0 0 0.12 0.2 0.3 0.27 0.19 0.35 0.11 0.08 0.09 0.08 0.1 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0.06 0.11 0 0.05 0 0 0 0.07 0.08
Mitochondria 0.87 0.86 0.44 0.65 0.49 0.86 0.67 0.79 0.89 0.84 0.85 0.9 0.61 0.51 0.3 0.08 0.19 0.12 0.68 0.75 0.77 0.69 0.64 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0.06 0.05 0.23 0.12 0 0.05 0 0 0.06 0 0 0.07 0.1 0.22 0.14 0.11 0.12 0.07 0 0.05 0.07 0.06 0.06
SpindlePole 0 0 0.2 0.1 0.15 0.06 0.1 0.07 0.06 0.1 0.05 0.05 0.08 0 0.05 0.13 0.14 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.11 0.11 0.07 0.19 0.07 0.13 0.08 0.07 0.05 0.05 0 0.05 0.09 0.1 0.22 0.17 0.23 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 7 0 2 0 0 0 0 1 0 0 21 12 7 6 0 0 3 9 2 5 19 20
Bud 4 20 6 6 3 9 4 16 12 21 11 11 5 4 8 4 1 1 0 4 0 1 3 1
Bud Neck 0 0 2 0 2 0 0 0 0 2 0 1 0 0 0 0 1 0 0 2 0 1 8 7
Bud Site 2 0 0 1 0 5 2 5 2 9 5 6 0 0 0 0 0 2 – – – – – –
Cell Periphery 6 20 5 10 6 0 5 5 1 4 1 1 14 21 19 8 2 0 0 1 0 1 0 2
Cytoplasm 6 26 8 19 18 27 17 31 10 18 20 8 32 54 77 15 1 3 0 2 0 3 1 1
Endoplasmic Reticulum 2 8 6 0 2 3 1 1 2 1 1 1 14 20 18 6 3 3 0 1 0 0 1 5
Endosome 15 34 14 64 70 11 69 49 24 17 7 2 36 89 153 27 7 9 30 39 18 33 59 59
Golgi 4 5 3 2 4 0 3 1 1 0 0 0 24 22 14 6 4 0 12 20 4 13 40 45
Mitochondria 218 414 54 161 128 268 236 404 344 303 203 196 184 226 150 8 7 3 184 394 166 290 381 384
Nucleus 2 0 0 1 6 12 10 12 4 11 3 4 0 1 1 6 0 0 0 0 0 0 1 3
Nuclear Periphery 6 1 0 0 0 1 13 11 5 6 3 5 4 1 0 1 0 0 5 0 1 6 6 5
Nucleolus 2 0 1 1 0 2 1 11 9 6 3 5 0 0 0 0 1 0 0 1 0 2 1 2
Peroxisomes 12 27 6 58 31 5 19 19 15 20 3 3 20 43 110 14 4 3 19 17 11 30 38 39
SpindlePole 9 10 25 24 39 20 34 38 23 36 13 11 25 12 23 13 5 4 7 5 2 10 15 5
Vac/Vac Membrane 28 53 14 17 49 21 45 40 27 17 13 7 14 40 51 22 6 6 3 19 2 15 16 15
Unique Cell Count 252 480 124 247 260 313 354 509 385 362 239 218 304 446 503 101 36 26 272 526 216 422 600 606
Labelled Cell Count 316 624 151 364 360 384 459 643 479 472 286 261 393 545 631 136 42 34 272 526 216 422 600 606


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.2 6.0 5.6 6.1 4.5 5.5 5.6 4.9 5.1 5.2 4.9 5.6 5.7 5.7 6.2 6.4 6.0 4.9 4.9 5.2
Std Deviation (1e-4) 0.8 0.8 1.3 1.4 1.5 1.3 1.3 1.6 1.1 1.6 1.7 1.3 1.0 1.0 1.1 1.3 1.1 1.7 1.2 1.1 1.4
Intensity Change (Log2) – – – -0.1 0.02 -0.42 -0.12 -0.1 -0.27 -0.22 -0.21 -0.28 -0.09 -0.08 -0.06 0.06 0.11 0.02 -0.29 -0.28 -0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0.5 0 0 0.1 0 0
Bud 0 0 0 0 0 0 0.4 -0.1 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.3 0.3 0 1.2 0 0
Cytoplasm 0.4 0.2 0.8 -0.7 -0.2 0 -0.6 0.6 -1.2 1.3 1.8 2.6 2.1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -0.1 -0.2 0 0.4 0 0
Endosome 3.3 3.5 0 2.1 -0.6 -1.9 -2.6 -3.2 -4.4 0.2 2.2 4.3 3.0 0 0
Golgi 0 0 0 0 0 0 0 0 0 2.1 1.2 0 0 0 0
Mitochondria 4.0 1.0 0 4.5 8.0 10.7 8.7 8.2 9.3 3.2 1.4 -2.9 -5.9 -2.6 -3.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 4.5 2.2 0 0.2 0 -0.5 0.3 0 0 0.7 1.7 4.4 2.4 0 0
SpindlePole -2.8 -1.3 0 -3.1 -4.2 -4.7 -3.0 -4.3 -4.4 -3.5 -7.0 -5.8 -1.5 -0.8 -0.6
Vacuole -1.4 1.9 -1.6 0.4 -1.2 -1.5 -2.6 -2.0 -3.0 -2.5 -0.8 -0.4 2.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5951 0.8679 0.6955 0.4996 0.3928 0.9935 1.7733 2.459 2.0434 1.9662 1.8023 2.199 4.9348 5.046 4.4964 5.5289 4.9248 4.7978
Actin 0.0059 0.0026 0.0087 0.0048 0.0021 0.0032 0.0028 0.0045 0.0037 0.0193 0.0305 0.0034 0.0246 0.0299 0.0418 0.0262 0.0019 0.0172
Bud 0.0006 0.0017 0.0006 0.0007 0.0012 0.0003 0.0004 0.0009 0.0009 0.0024 0.0017 0.0005 0.0011 0.0035 0.0044 0.0006 0.0012 0.0007
Bud Neck 0.0005 0.0004 0.0011 0.0037 0.0004 0.0005 0.0007 0.0006 0.0004 0.0007 0.0004 0.0008 0.0007 0.0038 0.0009 0.0012 0.0006 0.0044
Bud Periphery 0.0018 0.0026 0.002 0.0016 0.0054 0.0012 0.0008 0.0018 0.0018 0.0094 0.006 0.0011 0.0019 0.0052 0.0066 0.0016 0.0058 0.0013
Bud Site 0.0015 0.0049 0.0027 0.0029 0.0019 0.0005 0.0012 0.0052 0.0025 0.0024 0.0014 0.0006 0.0034 0.0563 0.0092 0.0029 0.0019 0.0015
Cell Periphery 0.0006 0.0006 0.0006 0.0004 0.0003 0.0004 0.0004 0.0009 0.0004 0.0009 0.0004 0.0002 0.0009 0.0049 0.0018 0.0005 0.0197 0.0007
Cytoplasm 0.0047 0.0066 0.0026 0.0008 0.0024 0.0023 0.0051 0.0036 0.0109 0.0047 0.0048 0.0041 0.0105 0.0256 0.0184 0.001 0.0143 0.0163
Cytoplasmic Foci 0.0362 0.039 0.0326 0.0184 0.0453 0.0233 0.0328 0.0294 0.0212 0.0218 0.0505 0.0277 0.0512 0.0427 0.0437 0.027 0.0451 0.0448
Eisosomes 0.0004 0.0004 0.0005 0.0004 0.0002 0.0003 0.0002 0.0006 0.0003 0.0005 0.0005 0.0002 0.0007 0.0038 0.0013 0.0003 0.0006 0.0004
Endoplasmic Reticulum 0.002 0.0015 0.0004 0.0002 0.001 0.001 0.0021 0.0009 0.0007 0.0017 0.0006 0.001 0.0104 0.0019 0.0042 0.0003 0.0041 0.0024
Endosome 0.0979 0.0866 0.0511 0.0318 0.0698 0.0493 0.11 0.0732 0.0566 0.0475 0.065 0.0502 0.1038 0.0825 0.0656 0.0302 0.0919 0.0748
Golgi 0.0612 0.0642 0.0535 0.0262 0.0421 0.0463 0.066 0.072 0.0607 0.0392 0.0597 0.0641 0.085 0.0692 0.053 0.0368 0.1022 0.0746
Lipid Particles 0.0316 0.0187 0.0167 0.0037 0.0048 0.0278 0.0298 0.0064 0.0084 0.0068 0.007 0.0088 0.0649 0.0107 0.017 0.0144 0.0542 0.0364
Mitochondria 0.6964 0.7027 0.7554 0.8703 0.7766 0.7809 0.6991 0.7612 0.7849 0.8139 0.7161 0.7883 0.5018 0.5829 0.6752 0.7695 0.538 0.6359
None 0.003 0.0034 0.0095 0.0007 0.0006 0.0042 0.0036 0.0014 0.0028 0.0025 0.001 0.0026 0.0182 0.0072 0.0081 0.001 0.0018 0.0182
Nuclear Periphery 0.0039 0.0014 0.0003 0.0001 0.0012 0.0037 0.0008 0.0006 0.0002 0.0002 0.0002 0.0019 0.0237 0.0003 0.0034 0.0002 0.0245 0.0009
Nucleolus 0.0006 0.0005 0.0007 0.0003 0.0002 0.0009 0.0005 0.0002 0.0002 0.0002 0.0002 0.0001 0.0017 0.0008 0.0012 0.0002 0.0006 0.0009
Nucleus 0.0005 0.0006 0.0005 0.0003 0.0006 0.0017 0.0007 0.0005 0.0003 0.0003 0.0002 0.0004 0.0032 0.0007 0.0126 0.0002 0.0226 0.0006
Peroxisomes 0.032 0.0406 0.0523 0.0289 0.0355 0.0433 0.0306 0.0297 0.0348 0.0202 0.0472 0.0388 0.0651 0.052 0.0208 0.0803 0.0463 0.0589
Punctate Nuclear 0.0003 0.0002 0.0005 0.0002 0.0004 0.0014 0.0005 0.0002 0.0002 0.0001 0.0002 0.0001 0.0076 0.0012 0.0017 0.0002 0.0021 0.0014
Vacuole 0.011 0.0118 0.0046 0.0021 0.0039 0.0035 0.0071 0.0035 0.0035 0.0026 0.0037 0.0024 0.0096 0.0105 0.0047 0.0026 0.0117 0.0046
Vacuole Periphery 0.0075 0.0089 0.0031 0.0016 0.0041 0.0041 0.0048 0.003 0.0047 0.0023 0.0031 0.0029 0.0101 0.0045 0.0045 0.0029 0.0089 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.9865 28.5906 17.2376 21.4605 19.4377 9.9891 20.3259 19.5109 19.096 18.9165
Translational Efficiency 0.9302 0.9523 1.148 0.6866 1.2251 1.8038 1.034 1.0571 1.142 1.3749

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1066 416 224 89 1385 2375 1840 1517 2451 2791 2064 1606

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 653.51 742.17 942.81 938.45 668.10 806.95 910.89 961.17 661.75 797.29 914.35 959.91
Standard Deviation 70.26 94.66 104.27 111.94 79.12 105.73 102.71 133.23 75.74 106.68 103.36 132.24
Intensity Change Log 2 — 0.183540 0.528758 0.522071 — 0.272415 0.447213 0.524728 — 0.229152 0.488111 0.523414

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000515 0.004979 0.007522 0.002200 0.000647 0.001188 0.006392 0.016271 0.000590 0.001753 0.006514 0.015491
Bud Neck 0.014520 0.013343 0.013253 0.027041 0.006207 0.005134 0.009267 0.002964 0.009823 0.006357 0.009699 0.004298
Bud Site 0.009240 0.028076 0.051633 0.061278 0.007066 0.010041 0.071113 0.073281 0.008011 0.012729 0.068999 0.072616
Cell Periphery 0.000562 0.000848 0.000240 0.000508 0.000635 0.000323 0.000239 0.000849 0.000603 0.000402 0.000239 0.000830
Cytoplasm 0.010088 0.009586 0.031977 0.008644 0.008975 0.004462 0.031260 0.018692 0.009459 0.005226 0.031338 0.018135
Cytoplasmic Foci 0.063296 0.071734 0.185229* 0.100960 0.078153 0.053737 0.124260 0.061672 0.071691 0.056419 0.130877 0.063850
Eisosomes 0.000142 0.000157 0.000155 0.000168 0.000120 0.000090 0.000113 0.000162 0.000130 0.000100 0.000117 0.000162
Endoplasmic Reticulum 0.000464 0.000732 0.011967 0.002538 0.000908 0.000258 0.002735 0.001006 0.000715 0.000329 0.003737 0.001091
Endosome 0.063313 0.114780 0.110293 0.175128 0.065777 0.098784 0.107882 0.072183 0.064705 0.101168 0.108144 0.077888
Golgi 0.095086 0.137028* 0.078341 0.077505 0.133580* 0.164086* 0.126642* 0.178182* 0.116838* 0.160053* 0.121400* 0.172603*
Lipid Particles 0.006191 0.006628 0.012987 0.016160 0.012930 0.003108 0.002059 0.000229 0.009999 0.003633 0.003245 0.001112
Mitochondria 0.652519* 0.483299* 0.053013 0.292912* 0.595550* 0.583509* 0.272804* 0.456944* 0.620327* 0.568573* 0.248950* 0.447854*
Mitotic Spindle 0.006213 0.010641 0.141501* 0.070746 0.008970 0.009874 0.075934 0.028504 0.007771 0.009988 0.083050 0.030845
None 0.000784 0.001429 0.000411 0.000204 0.000892 0.000304 0.000255 0.000220 0.000845 0.000472 0.000272 0.000220
Nuclear Periphery 0.000094 0.000084 0.001631 0.003297 0.000347 0.000052 0.000277 0.000052 0.000237 0.000057 0.000424 0.000232
Nuclear Periphery Foci 0.000107 0.000217 0.006150 0.005011 0.000354 0.000137 0.000634 0.000102 0.000247 0.000149 0.001232 0.000374
Nucleolus 0.001128 0.001374 0.005627 0.000783 0.000908 0.000299 0.000689 0.000067 0.001004 0.000459 0.001225 0.000107
Nucleus 0.002432 0.002114 0.036157 0.003244 0.001569 0.000688 0.005647 0.000317 0.001945 0.000901 0.008958 0.000479
Peroxisomes 0.054850 0.088552 0.124791* 0.063537 0.051186 0.031251 0.058573 0.053749 0.052780 0.039792 0.065760 0.054291
Vacuole 0.006930 0.014356 0.106597 0.057096 0.009502 0.018617 0.078751 0.018984 0.008383 0.017982 0.081773 0.021096
Vacuole Periphery 0.011526 0.010044 0.020524 0.031039 0.015722 0.014057 0.024475 0.015570 0.013897 0.013459 0.024046 0.016427

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.91 -6.22 -3.63 2.24 4.43 -2.33 -8.80 -9.21 -8.85 -5.43 -4.61 -10.07 -9.30 -8.49 -5.22
Bud Neck 0.33 0.34 -1.82 -1.90 -1.96 1.10 -3.23 3.65 2.97 9.15 2.91 -0.07 4.92 2.35 7.20
Bud Site -4.67 -7.91 -3.78 -2.33 -0.67 -2.24 -19.73 -16.42 -15.70 -0.43 -3.90 -20.92 -16.81 -15.33 -0.77
Cell Periphery -1.73 3.70 0.47 2.06 -3.14 1.19 1.55 -0.79 -4.86 -6.51 1.27 2.44 -1.31 -4.20 -6.67
Cytoplasm 0.22 -4.51 0.56 0.29 4.59 3.42 -10.66 -5.51 -9.93 5.82 3.91 -11.73 -5.56 -9.34 6.54
Cytoplasmic Foci -1.21 -10.85 -2.99 -2.29 4.92 7.07 -10.62 4.50 -2.72 16.64 5.68 -15.62 2.72 -2.61 18.01
Eisosomes -0.67 -1.44 -1.14 -0.50 -0.20 2.23 0.47 -2.73 -8.24 -5.48 3.09 1.11 -2.78 -7.25 -5.11
Endoplasmic Reticulum -0.84 -6.79 -3.37 -2.64 5.33 3.00 -4.48 -0.38 -4.66 4.49 2.77 -7.70 -1.91 -4.70 6.65
Endosome -5.86 -4.57 -5.97 -3.01 -3.46 -7.54 -9.30 -1.53 6.69 8.62 -9.86 -11.23 -3.59 5.93 7.26
Golgi -4.16 2.63 1.37 3.94 -0.39 -4.59 1.42 -5.91 -1.98 -8.03 -8.27 -0.39 -8.78 -1.86 -8.50
Lipid Particles -0.18 -3.36 -1.78 -1.55 -0.35 5.80 6.55 7.92 5.57 5.92 5.58 6.23 8.71 3.83 4.72
Mitochondria 7.87 48.05* 10.02 4.92 -6.89 0.98 27.81 10.58 11.10 -16.39 5.26 39.35* 15.24 11.02 -18.51
Mitotic Spindle -2.20 -8.85 -3.95 -3.67 3.10 -0.66 -17.50 -8.82 -8.56 11.19 -2.04 -19.89 -10.41 -9.31 12.12
None -0.73 1.31 2.80 1.47 3.01 3.14 3.49 3.71 1.70 1.73 2.00 4.10 4.59 1.92 2.93
Nuclear Periphery 0.29 -6.01 -1.86 -1.86 -0.90 2.07 0.33 2.06 -0.07 7.63 2.22 -2.38 0.03 -1.80 2.08
Nuclear Periphery Foci -1.50 -4.82 -1.64 -1.60 0.82 3.16 -2.78 4.05 0.89 5.61 2.10 -5.92 -0.76 -1.33 4.11
Nucleolus -0.24 -3.24 0.81 0.65 3.43 3.19 0.92 4.96 2.10 3.74 2.53 -1.37 5.21 2.27 5.04
Nucleus 0.24 -4.93 -0.59 -0.66 4.76 2.19 -4.34 3.84 1.08 5.57 2.57 -6.18 4.54 1.19 7.36
Peroxisomes -3.53 -4.95 -0.68 1.64 3.22 5.14 -1.70 -0.49 -5.44 1.12 3.93 -3.21 -0.31 -3.53 2.47
Vacuole -2.74 -10.01 -4.80 -4.00 3.91 -6.47 -22.13 -5.98 -0.46 17.81 -8.00 -24.72 -8.17 -1.94 18.64
Vacuole Periphery 0.93 -2.42 -3.08 -3.29 -1.74 1.23 -4.92 0.10 -1.04 4.81 0.42 -6.84 -1.74 -2.12 4.20
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Gtf1

Gtf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available