Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 7 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 21 | 12 | 7 | 6 | 0 | 0 | 3 | 9 | 2 | 5 | 19 | 20 |
Bud | 4 | 20 | 6 | 6 | 3 | 9 | 4 | 16 | 12 | 21 | 11 | 11 | 5 | 4 | 8 | 4 | 1 | 1 | 0 | 4 | 0 | 1 | 3 | 1 |
Bud Neck | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 8 | 7 |
Bud Site | 2 | 0 | 0 | 1 | 0 | 5 | 2 | 5 | 2 | 9 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 6 | 20 | 5 | 10 | 6 | 0 | 5 | 5 | 1 | 4 | 1 | 1 | 14 | 21 | 19 | 8 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 2 |
Cytoplasm | 6 | 26 | 8 | 19 | 18 | 27 | 17 | 31 | 10 | 18 | 20 | 8 | 32 | 54 | 77 | 15 | 1 | 3 | 0 | 2 | 0 | 3 | 1 | 1 |
Endoplasmic Reticulum | 2 | 8 | 6 | 0 | 2 | 3 | 1 | 1 | 2 | 1 | 1 | 1 | 14 | 20 | 18 | 6 | 3 | 3 | 0 | 1 | 0 | 0 | 1 | 5 |
Endosome | 15 | 34 | 14 | 64 | 70 | 11 | 69 | 49 | 24 | 17 | 7 | 2 | 36 | 89 | 153 | 27 | 7 | 9 | 30 | 39 | 18 | 33 | 59 | 59 |
Golgi | 4 | 5 | 3 | 2 | 4 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 24 | 22 | 14 | 6 | 4 | 0 | 12 | 20 | 4 | 13 | 40 | 45 |
Mitochondria | 218 | 414 | 54 | 161 | 128 | 268 | 236 | 404 | 344 | 303 | 203 | 196 | 184 | 226 | 150 | 8 | 7 | 3 | 184 | 394 | 166 | 290 | 381 | 384 |
Nucleus | 2 | 0 | 0 | 1 | 6 | 12 | 10 | 12 | 4 | 11 | 3 | 4 | 0 | 1 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Nuclear Periphery | 6 | 1 | 0 | 0 | 0 | 1 | 13 | 11 | 5 | 6 | 3 | 5 | 4 | 1 | 0 | 1 | 0 | 0 | 5 | 0 | 1 | 6 | 6 | 5 |
Nucleolus | 2 | 0 | 1 | 1 | 0 | 2 | 1 | 11 | 9 | 6 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 2 |
Peroxisomes | 12 | 27 | 6 | 58 | 31 | 5 | 19 | 19 | 15 | 20 | 3 | 3 | 20 | 43 | 110 | 14 | 4 | 3 | 19 | 17 | 11 | 30 | 38 | 39 |
SpindlePole | 9 | 10 | 25 | 24 | 39 | 20 | 34 | 38 | 23 | 36 | 13 | 11 | 25 | 12 | 23 | 13 | 5 | 4 | 7 | 5 | 2 | 10 | 15 | 5 |
Vac/Vac Membrane | 28 | 53 | 14 | 17 | 49 | 21 | 45 | 40 | 27 | 17 | 13 | 7 | 14 | 40 | 51 | 22 | 6 | 6 | 3 | 19 | 2 | 15 | 16 | 15 |
Unique Cell Count | 252 | 480 | 124 | 247 | 260 | 313 | 354 | 509 | 385 | 362 | 239 | 218 | 304 | 446 | 503 | 101 | 36 | 26 | 272 | 526 | 216 | 422 | 600 | 606 |
Labelled Cell Count | 316 | 624 | 151 | 364 | 360 | 384 | 459 | 643 | 479 | 472 | 286 | 261 | 393 | 545 | 631 | 136 | 42 | 34 | 272 | 526 | 216 | 422 | 600 | 606 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.2 | 6.0 | 5.6 | 6.1 | 4.5 | 5.5 | 5.6 | 4.9 | 5.1 | 5.2 | 4.9 | 5.6 | 5.7 | 5.7 | 6.2 | 6.4 | 6.0 | 4.9 | 4.9 | 5.2 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.3 | 1.4 | 1.5 | 1.3 | 1.3 | 1.6 | 1.1 | 1.6 | 1.7 | 1.3 | 1.0 | 1.0 | 1.1 | 1.3 | 1.1 | 1.7 | 1.2 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.02 | -0.42 | -0.12 | -0.1 | -0.27 | -0.22 | -0.21 | -0.28 | -0.09 | -0.08 | -0.06 | 0.06 | 0.11 | 0.02 | -0.29 | -0.28 | -0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0 | 0 | 0.1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | -0.1 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 0.3 | 0 | 1.2 | 0 | 0 |
Cytoplasm | 0.4 | 0.2 | 0.8 | -0.7 | -0.2 | 0 | -0.6 | 0.6 | -1.2 | 1.3 | 1.8 | 2.6 | 2.1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | -0.2 | 0 | 0.4 | 0 | 0 |
Endosome | 3.3 | 3.5 | 0 | 2.1 | -0.6 | -1.9 | -2.6 | -3.2 | -4.4 | 0.2 | 2.2 | 4.3 | 3.0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.2 | 0 | 0 | 0 | 0 |
Mitochondria | 4.0 | 1.0 | 0 | 4.5 | 8.0 | 10.7 | 8.7 | 8.2 | 9.3 | 3.2 | 1.4 | -2.9 | -5.9 | -2.6 | -3.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 4.5 | 2.2 | 0 | 0.2 | 0 | -0.5 | 0.3 | 0 | 0 | 0.7 | 1.7 | 4.4 | 2.4 | 0 | 0 |
SpindlePole | -2.8 | -1.3 | 0 | -3.1 | -4.2 | -4.7 | -3.0 | -4.3 | -4.4 | -3.5 | -7.0 | -5.8 | -1.5 | -0.8 | -0.6 |
Vacuole | -1.4 | 1.9 | -1.6 | 0.4 | -1.2 | -1.5 | -2.6 | -2.0 | -3.0 | -2.5 | -0.8 | -0.4 | 2.1 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5951 | 0.8679 | 0.6955 | 0.4996 | 0.3928 | 0.9935 | 1.7733 | 2.459 | 2.0434 | 1.9662 | 1.8023 | 2.199 | 4.9348 | 5.046 | 4.4964 | 5.5289 | 4.9248 | 4.7978 |
Actin | 0.0059 | 0.0026 | 0.0087 | 0.0048 | 0.0021 | 0.0032 | 0.0028 | 0.0045 | 0.0037 | 0.0193 | 0.0305 | 0.0034 | 0.0246 | 0.0299 | 0.0418 | 0.0262 | 0.0019 | 0.0172 |
Bud | 0.0006 | 0.0017 | 0.0006 | 0.0007 | 0.0012 | 0.0003 | 0.0004 | 0.0009 | 0.0009 | 0.0024 | 0.0017 | 0.0005 | 0.0011 | 0.0035 | 0.0044 | 0.0006 | 0.0012 | 0.0007 |
Bud Neck | 0.0005 | 0.0004 | 0.0011 | 0.0037 | 0.0004 | 0.0005 | 0.0007 | 0.0006 | 0.0004 | 0.0007 | 0.0004 | 0.0008 | 0.0007 | 0.0038 | 0.0009 | 0.0012 | 0.0006 | 0.0044 |
Bud Periphery | 0.0018 | 0.0026 | 0.002 | 0.0016 | 0.0054 | 0.0012 | 0.0008 | 0.0018 | 0.0018 | 0.0094 | 0.006 | 0.0011 | 0.0019 | 0.0052 | 0.0066 | 0.0016 | 0.0058 | 0.0013 |
Bud Site | 0.0015 | 0.0049 | 0.0027 | 0.0029 | 0.0019 | 0.0005 | 0.0012 | 0.0052 | 0.0025 | 0.0024 | 0.0014 | 0.0006 | 0.0034 | 0.0563 | 0.0092 | 0.0029 | 0.0019 | 0.0015 |
Cell Periphery | 0.0006 | 0.0006 | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0009 | 0.0004 | 0.0009 | 0.0004 | 0.0002 | 0.0009 | 0.0049 | 0.0018 | 0.0005 | 0.0197 | 0.0007 |
Cytoplasm | 0.0047 | 0.0066 | 0.0026 | 0.0008 | 0.0024 | 0.0023 | 0.0051 | 0.0036 | 0.0109 | 0.0047 | 0.0048 | 0.0041 | 0.0105 | 0.0256 | 0.0184 | 0.001 | 0.0143 | 0.0163 |
Cytoplasmic Foci | 0.0362 | 0.039 | 0.0326 | 0.0184 | 0.0453 | 0.0233 | 0.0328 | 0.0294 | 0.0212 | 0.0218 | 0.0505 | 0.0277 | 0.0512 | 0.0427 | 0.0437 | 0.027 | 0.0451 | 0.0448 |
Eisosomes | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0007 | 0.0038 | 0.0013 | 0.0003 | 0.0006 | 0.0004 |
Endoplasmic Reticulum | 0.002 | 0.0015 | 0.0004 | 0.0002 | 0.001 | 0.001 | 0.0021 | 0.0009 | 0.0007 | 0.0017 | 0.0006 | 0.001 | 0.0104 | 0.0019 | 0.0042 | 0.0003 | 0.0041 | 0.0024 |
Endosome | 0.0979 | 0.0866 | 0.0511 | 0.0318 | 0.0698 | 0.0493 | 0.11 | 0.0732 | 0.0566 | 0.0475 | 0.065 | 0.0502 | 0.1038 | 0.0825 | 0.0656 | 0.0302 | 0.0919 | 0.0748 |
Golgi | 0.0612 | 0.0642 | 0.0535 | 0.0262 | 0.0421 | 0.0463 | 0.066 | 0.072 | 0.0607 | 0.0392 | 0.0597 | 0.0641 | 0.085 | 0.0692 | 0.053 | 0.0368 | 0.1022 | 0.0746 |
Lipid Particles | 0.0316 | 0.0187 | 0.0167 | 0.0037 | 0.0048 | 0.0278 | 0.0298 | 0.0064 | 0.0084 | 0.0068 | 0.007 | 0.0088 | 0.0649 | 0.0107 | 0.017 | 0.0144 | 0.0542 | 0.0364 |
Mitochondria | 0.6964 | 0.7027 | 0.7554 | 0.8703 | 0.7766 | 0.7809 | 0.6991 | 0.7612 | 0.7849 | 0.8139 | 0.7161 | 0.7883 | 0.5018 | 0.5829 | 0.6752 | 0.7695 | 0.538 | 0.6359 |
None | 0.003 | 0.0034 | 0.0095 | 0.0007 | 0.0006 | 0.0042 | 0.0036 | 0.0014 | 0.0028 | 0.0025 | 0.001 | 0.0026 | 0.0182 | 0.0072 | 0.0081 | 0.001 | 0.0018 | 0.0182 |
Nuclear Periphery | 0.0039 | 0.0014 | 0.0003 | 0.0001 | 0.0012 | 0.0037 | 0.0008 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0019 | 0.0237 | 0.0003 | 0.0034 | 0.0002 | 0.0245 | 0.0009 |
Nucleolus | 0.0006 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0009 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0017 | 0.0008 | 0.0012 | 0.0002 | 0.0006 | 0.0009 |
Nucleus | 0.0005 | 0.0006 | 0.0005 | 0.0003 | 0.0006 | 0.0017 | 0.0007 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0032 | 0.0007 | 0.0126 | 0.0002 | 0.0226 | 0.0006 |
Peroxisomes | 0.032 | 0.0406 | 0.0523 | 0.0289 | 0.0355 | 0.0433 | 0.0306 | 0.0297 | 0.0348 | 0.0202 | 0.0472 | 0.0388 | 0.0651 | 0.052 | 0.0208 | 0.0803 | 0.0463 | 0.0589 |
Punctate Nuclear | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0014 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0076 | 0.0012 | 0.0017 | 0.0002 | 0.0021 | 0.0014 |
Vacuole | 0.011 | 0.0118 | 0.0046 | 0.0021 | 0.0039 | 0.0035 | 0.0071 | 0.0035 | 0.0035 | 0.0026 | 0.0037 | 0.0024 | 0.0096 | 0.0105 | 0.0047 | 0.0026 | 0.0117 | 0.0046 |
Vacuole Periphery | 0.0075 | 0.0089 | 0.0031 | 0.0016 | 0.0041 | 0.0041 | 0.0048 | 0.003 | 0.0047 | 0.0023 | 0.0031 | 0.0029 | 0.0101 | 0.0045 | 0.0045 | 0.0029 | 0.0089 | 0.0032 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.9865 | 28.5906 | 17.2376 | 21.4605 | 19.4377 | 9.9891 | 20.3259 | 19.5109 | 19.096 | 18.9165 |
Translational Efficiency | 0.9302 | 0.9523 | 1.148 | 0.6866 | 1.2251 | 1.8038 | 1.034 | 1.0571 | 1.142 | 1.3749 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1066 | 416 | 224 | 89 | 1385 | 2375 | 1840 | 1517 | 2451 | 2791 | 2064 | 1606 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 653.51 | 742.17 | 942.81 | 938.45 | 668.10 | 806.95 | 910.89 | 961.17 | 661.75 | 797.29 | 914.35 | 959.91 |
Standard Deviation | 70.26 | 94.66 | 104.27 | 111.94 | 79.12 | 105.73 | 102.71 | 133.23 | 75.74 | 106.68 | 103.36 | 132.24 |
Intensity Change Log 2 | — | 0.183540 | 0.528758 | 0.522071 | — | 0.272415 | 0.447213 | 0.524728 | — | 0.229152 | 0.488111 | 0.523414 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000515 | 0.004979 | 0.007522 | 0.002200 | 0.000647 | 0.001188 | 0.006392 | 0.016271 | 0.000590 | 0.001753 | 0.006514 | 0.015491 |
Bud Neck | 0.014520 | 0.013343 | 0.013253 | 0.027041 | 0.006207 | 0.005134 | 0.009267 | 0.002964 | 0.009823 | 0.006357 | 0.009699 | 0.004298 |
Bud Site | 0.009240 | 0.028076 | 0.051633 | 0.061278 | 0.007066 | 0.010041 | 0.071113 | 0.073281 | 0.008011 | 0.012729 | 0.068999 | 0.072616 |
Cell Periphery | 0.000562 | 0.000848 | 0.000240 | 0.000508 | 0.000635 | 0.000323 | 0.000239 | 0.000849 | 0.000603 | 0.000402 | 0.000239 | 0.000830 |
Cytoplasm | 0.010088 | 0.009586 | 0.031977 | 0.008644 | 0.008975 | 0.004462 | 0.031260 | 0.018692 | 0.009459 | 0.005226 | 0.031338 | 0.018135 |
Cytoplasmic Foci | 0.063296 | 0.071734 | 0.185229* | 0.100960 | 0.078153 | 0.053737 | 0.124260 | 0.061672 | 0.071691 | 0.056419 | 0.130877 | 0.063850 |
Eisosomes | 0.000142 | 0.000157 | 0.000155 | 0.000168 | 0.000120 | 0.000090 | 0.000113 | 0.000162 | 0.000130 | 0.000100 | 0.000117 | 0.000162 |
Endoplasmic Reticulum | 0.000464 | 0.000732 | 0.011967 | 0.002538 | 0.000908 | 0.000258 | 0.002735 | 0.001006 | 0.000715 | 0.000329 | 0.003737 | 0.001091 |
Endosome | 0.063313 | 0.114780 | 0.110293 | 0.175128 | 0.065777 | 0.098784 | 0.107882 | 0.072183 | 0.064705 | 0.101168 | 0.108144 | 0.077888 |
Golgi | 0.095086 | 0.137028* | 0.078341 | 0.077505 | 0.133580* | 0.164086* | 0.126642* | 0.178182* | 0.116838* | 0.160053* | 0.121400* | 0.172603* |
Lipid Particles | 0.006191 | 0.006628 | 0.012987 | 0.016160 | 0.012930 | 0.003108 | 0.002059 | 0.000229 | 0.009999 | 0.003633 | 0.003245 | 0.001112 |
Mitochondria | 0.652519* | 0.483299* | 0.053013 | 0.292912* | 0.595550* | 0.583509* | 0.272804* | 0.456944* | 0.620327* | 0.568573* | 0.248950* | 0.447854* |
Mitotic Spindle | 0.006213 | 0.010641 | 0.141501* | 0.070746 | 0.008970 | 0.009874 | 0.075934 | 0.028504 | 0.007771 | 0.009988 | 0.083050 | 0.030845 |
None | 0.000784 | 0.001429 | 0.000411 | 0.000204 | 0.000892 | 0.000304 | 0.000255 | 0.000220 | 0.000845 | 0.000472 | 0.000272 | 0.000220 |
Nuclear Periphery | 0.000094 | 0.000084 | 0.001631 | 0.003297 | 0.000347 | 0.000052 | 0.000277 | 0.000052 | 0.000237 | 0.000057 | 0.000424 | 0.000232 |
Nuclear Periphery Foci | 0.000107 | 0.000217 | 0.006150 | 0.005011 | 0.000354 | 0.000137 | 0.000634 | 0.000102 | 0.000247 | 0.000149 | 0.001232 | 0.000374 |
Nucleolus | 0.001128 | 0.001374 | 0.005627 | 0.000783 | 0.000908 | 0.000299 | 0.000689 | 0.000067 | 0.001004 | 0.000459 | 0.001225 | 0.000107 |
Nucleus | 0.002432 | 0.002114 | 0.036157 | 0.003244 | 0.001569 | 0.000688 | 0.005647 | 0.000317 | 0.001945 | 0.000901 | 0.008958 | 0.000479 |
Peroxisomes | 0.054850 | 0.088552 | 0.124791* | 0.063537 | 0.051186 | 0.031251 | 0.058573 | 0.053749 | 0.052780 | 0.039792 | 0.065760 | 0.054291 |
Vacuole | 0.006930 | 0.014356 | 0.106597 | 0.057096 | 0.009502 | 0.018617 | 0.078751 | 0.018984 | 0.008383 | 0.017982 | 0.081773 | 0.021096 |
Vacuole Periphery | 0.011526 | 0.010044 | 0.020524 | 0.031039 | 0.015722 | 0.014057 | 0.024475 | 0.015570 | 0.013897 | 0.013459 | 0.024046 | 0.016427 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.91 | -6.22 | -3.63 | 2.24 | 4.43 | -2.33 | -8.80 | -9.21 | -8.85 | -5.43 | -4.61 | -10.07 | -9.30 | -8.49 | -5.22 |
Bud Neck | 0.33 | 0.34 | -1.82 | -1.90 | -1.96 | 1.10 | -3.23 | 3.65 | 2.97 | 9.15 | 2.91 | -0.07 | 4.92 | 2.35 | 7.20 |
Bud Site | -4.67 | -7.91 | -3.78 | -2.33 | -0.67 | -2.24 | -19.73 | -16.42 | -15.70 | -0.43 | -3.90 | -20.92 | -16.81 | -15.33 | -0.77 |
Cell Periphery | -1.73 | 3.70 | 0.47 | 2.06 | -3.14 | 1.19 | 1.55 | -0.79 | -4.86 | -6.51 | 1.27 | 2.44 | -1.31 | -4.20 | -6.67 |
Cytoplasm | 0.22 | -4.51 | 0.56 | 0.29 | 4.59 | 3.42 | -10.66 | -5.51 | -9.93 | 5.82 | 3.91 | -11.73 | -5.56 | -9.34 | 6.54 |
Cytoplasmic Foci | -1.21 | -10.85 | -2.99 | -2.29 | 4.92 | 7.07 | -10.62 | 4.50 | -2.72 | 16.64 | 5.68 | -15.62 | 2.72 | -2.61 | 18.01 |
Eisosomes | -0.67 | -1.44 | -1.14 | -0.50 | -0.20 | 2.23 | 0.47 | -2.73 | -8.24 | -5.48 | 3.09 | 1.11 | -2.78 | -7.25 | -5.11 |
Endoplasmic Reticulum | -0.84 | -6.79 | -3.37 | -2.64 | 5.33 | 3.00 | -4.48 | -0.38 | -4.66 | 4.49 | 2.77 | -7.70 | -1.91 | -4.70 | 6.65 |
Endosome | -5.86 | -4.57 | -5.97 | -3.01 | -3.46 | -7.54 | -9.30 | -1.53 | 6.69 | 8.62 | -9.86 | -11.23 | -3.59 | 5.93 | 7.26 |
Golgi | -4.16 | 2.63 | 1.37 | 3.94 | -0.39 | -4.59 | 1.42 | -5.91 | -1.98 | -8.03 | -8.27 | -0.39 | -8.78 | -1.86 | -8.50 |
Lipid Particles | -0.18 | -3.36 | -1.78 | -1.55 | -0.35 | 5.80 | 6.55 | 7.92 | 5.57 | 5.92 | 5.58 | 6.23 | 8.71 | 3.83 | 4.72 |
Mitochondria | 7.87 | 48.05* | 10.02 | 4.92 | -6.89 | 0.98 | 27.81 | 10.58 | 11.10 | -16.39 | 5.26 | 39.35* | 15.24 | 11.02 | -18.51 |
Mitotic Spindle | -2.20 | -8.85 | -3.95 | -3.67 | 3.10 | -0.66 | -17.50 | -8.82 | -8.56 | 11.19 | -2.04 | -19.89 | -10.41 | -9.31 | 12.12 |
None | -0.73 | 1.31 | 2.80 | 1.47 | 3.01 | 3.14 | 3.49 | 3.71 | 1.70 | 1.73 | 2.00 | 4.10 | 4.59 | 1.92 | 2.93 |
Nuclear Periphery | 0.29 | -6.01 | -1.86 | -1.86 | -0.90 | 2.07 | 0.33 | 2.06 | -0.07 | 7.63 | 2.22 | -2.38 | 0.03 | -1.80 | 2.08 |
Nuclear Periphery Foci | -1.50 | -4.82 | -1.64 | -1.60 | 0.82 | 3.16 | -2.78 | 4.05 | 0.89 | 5.61 | 2.10 | -5.92 | -0.76 | -1.33 | 4.11 |
Nucleolus | -0.24 | -3.24 | 0.81 | 0.65 | 3.43 | 3.19 | 0.92 | 4.96 | 2.10 | 3.74 | 2.53 | -1.37 | 5.21 | 2.27 | 5.04 |
Nucleus | 0.24 | -4.93 | -0.59 | -0.66 | 4.76 | 2.19 | -4.34 | 3.84 | 1.08 | 5.57 | 2.57 | -6.18 | 4.54 | 1.19 | 7.36 |
Peroxisomes | -3.53 | -4.95 | -0.68 | 1.64 | 3.22 | 5.14 | -1.70 | -0.49 | -5.44 | 1.12 | 3.93 | -3.21 | -0.31 | -3.53 | 2.47 |
Vacuole | -2.74 | -10.01 | -4.80 | -4.00 | 3.91 | -6.47 | -22.13 | -5.98 | -0.46 | 17.81 | -8.00 | -24.72 | -8.17 | -1.94 | 18.64 |
Vacuole Periphery | 0.93 | -2.42 | -3.08 | -3.29 | -1.74 | 1.23 | -4.92 | 0.10 | -1.04 | 4.81 | 0.42 | -6.84 | -1.74 | -2.12 | 4.20 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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