ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.98 0.96 0.88 0.79 0.76 0.66 0.66 0.57 0.59 0.99 0.99 0.99 0.95 0.96 0.95 0.95 0.96 0.94 0.89 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0.05 0.15 0.29 0.26 0.35 0.45 0.55 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.05 0.1 0.16 0.1 0.12 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 0 0 2
Bud 0 0 2 0 1 0 6 2 11 7 11 4 0 0 0 0 2 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 2 1 1 6 2 1 1 4 5 0 1 0 6 4 10 0 0 0 0 0 0
Cytoplasm 109 157 218 215 268 285 328 331 250 267 167 276 270 407 379 171 135 122 103 156 204 55 227 235
Endoplasmic Reticulum 0 1 5 0 0 1 1 0 0 4 0 9 3 3 1 5 0 3 0 0 2 0 0 0
Endosome 0 0 0 0 1 2 0 0 3 0 0 1 0 1 0 1 1 0 0 1 0 1 0 1
Golgi 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1 0 0 0
Mitochondria 6 2 1 9 13 50 118 113 132 181 160 227 0 0 0 3 0 2 0 0 0 0 0 2
Nucleus 0 0 0 0 3 3 8 8 9 9 3 9 0 1 3 4 0 3 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 2 2 0 1 0 3 2 5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 0 1 2 4 18 22 43 59 42 34 28 1 4 6 4 9 5 0 0 1 1 0 1
Unique Cell Count 112 160 226 219 279 325 413 433 380 402 293 470 273 411 383 180 140 129 108 163 218 62 234 252
Labelled Cell Count 115 160 233 230 293 362 490 500 467 515 382 566 274 417 389 195 152 145 108 163 218 62 234 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.4 7.3 6.2 6.5 5.6 5.3 5.4 5.1 4.9 4.4 4.4 7.8 7.9 7.8 10.0 10.4 9.9 6.0 8.0 8.5
Std Deviation (1e-4) 0.7 1.2 2.6 1.4 2.0 1.2 1.3 1.5 1.7 1.7 1.3 0.8 1.2 1.1 1.1 1.8 1.9 1.8 0.7 1.3 1.4
Intensity Change (Log2) – – – -0.23 -0.16 -0.37 -0.46 -0.44 -0.51 -0.58 -0.74 -0.72 0.1 0.11 0.09 0.46 0.52 0.44 -0.28 0.14 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8
Cytoplasm 1.1 -0.2 -3.6 -5.8 -6.5 -8.7 -8.6 -10.2 -10.3 1.8 2.3 2.1 -0.7 0 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.9 6.0 8.7 8.3 9.9 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 3.2 4.6 6.0 4.8 5.0 3.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9958 3.6647 3.1233 2.8488 2.3801 2.9698 1.5087 2.7771 2.7045 2.2129 2.2702 2.3797 0.5499 1.6438 1.4577 0.9659 1.8264 1.1453
Actin 0.0395 0.0002 0.0148 0.0028 0.0068 0.0005 0.0518 0.002 0.0093 0.0001 0.0003 0.0026 0.0041 0.0006 0.0097 0.026 0 0.0001
Bud 0.0003 0.0002 0.0002 0.0004 0.0061 0.0001 0.0018 0.0076 0.0005 0.0001 0.0001 0.0017 0.0001 0.0022 0.0001 0.0032 0 0
Bud Neck 0.0019 0.0001 0.0003 0.0006 0.001 0.0009 0.0036 0.0002 0.0004 0.0002 0.0004 0.0015 0.0002 0.0008 0.0002 0.0011 0.0002 0.0005
Bud Periphery 0.0004 0.0001 0.0002 0.0007 0.0089 0 0.002 0.0042 0.0003 0 0.0001 0.0021 0.0001 0.0006 0.0001 0.0038 0 0
Bud Site 0.0013 0.0024 0.0005 0.0022 0.0073 0.0001 0.0182 0.0031 0.0009 0.0001 0.0003 0.0007 0.0001 0.0075 0.0004 0.0068 0 0
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0005 0.0001 0 0 0 0.0001 0 0.0001 0 0.0002 0 0
Cytoplasm 0.7472 0.8952 0.8534 0.7887 0.7399 0.8838 0.6225 0.9381 0.9293 0.8681 0.8967 0.868 0.8568 0.9206 0.9217 0.8422 0.9556 0.9598
Cytoplasmic Foci 0.0101 0.004 0.0089 0.0123 0.0111 0.0066 0.0724 0.0058 0.0075 0.0072 0.015 0.019 0.007 0.0037 0.0042 0.0185 0.0039 0.0053
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0 0.0011 0 0 0 0 0 0.0001 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0052 0.0015 0.0024 0.0004 0.0035 0.0011 0.0052 0.0013 0.0023 0.0008 0.0038 0.0022 0.0039 0.0022 0.0025 0.0062 0.0018 0.0014
Endosome 0.0162 0.0012 0.0084 0.0067 0.014 0.0018 0.0458 0.0008 0.0076 0.0136 0.0202 0.019 0.0046 0.0026 0.002 0.0154 0.0044 0.0021
Golgi 0.0037 0.0001 0.0017 0.0081 0.0036 0.0001 0.0184 0.0002 0.0053 0.0078 0.0098 0.0097 0.001 0.0006 0.0004 0.006 0.0001 0
Lipid Particles 0.0027 0.0001 0.0011 0.0285 0.0015 0 0.015 0.0015 0.0031 0.0012 0.0302 0.0147 0.004 0.0005 0.0002 0.0035 0 0
Mitochondria 0.0019 0.0001 0.0013 0.0442 0.0199 0.0001 0.0176 0.0017 0.0026 0.0249 0.0032 0.0124 0.0007 0.0004 0.0001 0.0077 0.0001 0.0001
None 0.1166 0.0914 0.0923 0.005 0.1121 0.0951 0.0549 0.0273 0.0238 0.0618 0.0067 0.0151 0.0955 0.0492 0.0538 0.0286 0.0216 0.0212
Nuclear Periphery 0.0232 0.0005 0.0015 0.0003 0.0105 0.0007 0.0032 0.0007 0.001 0.0008 0.0024 0.0023 0.0063 0.0009 0.0009 0.0047 0.0019 0.001
Nucleolus 0.0007 0 0.0001 0.0011 0.0017 0 0.0009 0.0002 0 0 0.0002 0.001 0.0001 0.0002 0 0.0009 0 0
Nucleus 0.015 0.002 0.0033 0.0033 0.034 0.0079 0.0039 0.0022 0.0031 0.0052 0.0047 0.0123 0.0112 0.0038 0.0026 0.0072 0.0086 0.0073
Peroxisomes 0.0013 0 0.0076 0.0926 0.0048 0.0001 0.0363 0.0011 0.0015 0.006 0.0029 0.006 0.0018 0.0003 0.0001 0.0091 0 0
Punctate Nuclear 0.0105 0.0003 0.001 0.001 0.0074 0.0008 0.0078 0.0009 0.0007 0.0003 0.0007 0.0044 0.0011 0.0015 0.0004 0.006 0.0005 0.0007
Vacuole 0.0011 0.0004 0.0006 0.0007 0.004 0.0003 0.0146 0.0006 0.0007 0.0013 0.0018 0.0038 0.0009 0.0015 0.0004 0.0018 0.001 0.0004
Vacuole Periphery 0.0009 0 0.0001 0.0003 0.0016 0.0001 0.0027 0.0002 0.0003 0.0004 0.0006 0.0014 0.0005 0.0001 0.0001 0.0008 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.3889 26.2378 16.4274 25.957 16.0543 35.7514 30.8814 27.977 29.7917 28.0878
Translational Efficiency 1.3922 1.0411 1.267 0.931 1.7071 1.2201 1.0703 1.1138 1.2522 1.2327

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
261 127 427 385 1778 1449 288 1001 2039 1576 715 1386

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 819.56 933.71 1142.19 1095.33 830.95 884.54 1190.33 956.93 829.49 888.50 1161.58 995.37
Standard Deviation 111.96 125.59 135.27 161.59 116.37 103.93 149.94 170.40 115.87 106.69 143.32 179.07
Intensity Change Log 2 — 0.188125 0.478881 0.418444 — 0.090166 0.518528 0.203652 — 0.139639 0.498978 0.314302

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000365 0.001205 0.000573 0.001636 0.000084 0.000301 0.000550 0.001068 0.000120 0.000374 0.000564 0.001226
Bud Neck 0.004221 0.005111 0.002925 0.003299 0.001892 0.005283 0.002006 0.001525 0.002190 0.005269 0.002554 0.002018
Bud Site 0.009256 0.030312 0.005398 0.031351 0.003412 0.008982 0.006401 0.009751 0.004160 0.010701 0.005802 0.015751
Cell Periphery 0.000197 0.000082 0.000191 0.000215 0.000094 0.000081 0.000198 0.000124 0.000107 0.000081 0.000194 0.000149
Cytoplasm 0.566992* 0.668744* 0.416228* 0.550453* 0.746919* 0.662124* 0.466940* 0.720113* 0.723888* 0.662657* 0.436655* 0.672985*
Cytoplasmic Foci 0.186551* 0.123772 0.011860 0.029318 0.079468 0.105420 0.015140 0.011346 0.093175 0.106899 0.013182 0.016338
Eisosomes 0.000101 0.000036 0.000042 0.000039 0.000027 0.000042 0.000056 0.000017 0.000037 0.000042 0.000048 0.000023
Endoplasmic Reticulum 0.001630 0.000697 0.004693 0.004479 0.000937 0.000632 0.003668 0.000842 0.001026 0.000637 0.004280 0.001852
Endosome 0.010991 0.007726 0.004047 0.008226 0.003428 0.007906 0.004417 0.002750 0.004396 0.007891 0.004196 0.004271
Golgi 0.001476 0.003154 0.000158 0.032819 0.000435 0.002431 0.000159 0.000965 0.000568 0.002489 0.000158 0.009813
Lipid Particles 0.003372 0.003746 0.001844 0.001190 0.001176 0.001497 0.001682 0.000509 0.001457 0.001678 0.001779 0.000698
Mitochondria 0.007236 0.000430 0.000529 0.016947 0.000305 0.001651 0.000196 0.001837 0.001192 0.001553 0.000395 0.006034
Mitotic Spindle 0.000274 0.005728 0.007531 0.014729 0.001917 0.010574 0.001854 0.018950 0.001707 0.010184 0.005244 0.017777
None 0.000955 0.003025 0.003614 0.004669 0.005467 0.001368 0.003855 0.011503 0.004890 0.001502 0.003711 0.009604
Nuclear Periphery 0.000287 0.000148 0.001659 0.000499 0.000257 0.000265 0.001276 0.000514 0.000261 0.000255 0.001504 0.000510
Nuclear Periphery Foci 0.002030 0.001886 0.005268 0.004015 0.001017 0.000639 0.008389 0.002128 0.001147 0.000740 0.006525 0.002652
Nucleolus 0.000346 0.000220 0.000196 0.000096 0.000287 0.000384 0.000189 0.000201 0.000295 0.000370 0.000193 0.000171
Nucleus 0.088688 0.041343 0.379830* 0.095411 0.114178 0.088987 0.237446* 0.139069* 0.110915 0.085148 0.322478* 0.126942
Peroxisomes 0.002351 0.011759 0.000219 0.000283 0.000373 0.002788 0.000217 0.003184 0.000626 0.003511 0.000218 0.002378
Vacuole 0.111884 0.090609 0.152996 0.196782 0.037988 0.097960 0.245209* 0.072180 0.047447 0.097367 0.190139 0.106792
Vacuole Periphery 0.000796 0.000269 0.000196 0.003548 0.000340 0.000686 0.000153 0.001425 0.000398 0.000653 0.000179 0.002015

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.49 -3.49 -2.16 0.68 0.03 -2.51 -7.14 -3.20 -1.62 0.34 -2.74 -12.18 -4.20 -1.81 0.58
Bud Neck -0.30 -0.37 1.60 1.52 3.06 -5.66 -3.76 0.49 6.12 4.70 -5.40 -4.72 0.55 5.93 5.73
Bud Site -2.18 0.62 -1.11 1.55 -1.94 -4.45 -4.49 -3.95 1.16 0.04 -4.65 -4.25 -4.05 0.74 -1.42
Cell Periphery 3.93 -0.29 2.18 -3.39 4.45 2.30 -6.92 -1.78 -3.04 4.14 4.02 -8.50 -1.19 -4.18 6.18
Cytoplasm -2.86 11.55 5.83 7.52 -5.70 10.69 27.36 17.06 6.90 -14.95 8.11 38.93 20.45 11.64 -18.24
Cytoplasmic Foci 4.00 17.29 15.83 7.59 -4.75 -5.51 23.54 23.53 25.08 -1.32 -2.86 28.39 26.45 25.50 -4.41
Eisosomes 5.24 4.00 5.62 0.10 4.73 -6.37 -9.57 1.95 7.65 10.35 -1.86 -6.97 4.43 6.59 11.66
Endoplasmic Reticulum 2.35 -7.87 -0.05 -2.40 7.81 3.13 -9.99 -0.28 -3.99 10.01 3.96 -14.42 -1.00 -4.67 13.71
Endosome 1.61 4.16 5.05 2.50 2.85 -7.59 -0.92 5.26 10.75 5.62 -5.78 1.05 6.81 10.88 6.83
Golgi -0.83 3.08 -1.32 -0.79 -1.78 -2.64 4.19 0.56 2.72 -1.08 -2.68 5.08 -1.40 0.71 -1.91
Lipid Particles 0.05 1.86 2.81 0.85 2.91 -1.81 -5.16 2.47 4.07 8.20 -0.75 -4.61 2.62 2.50 9.34
Mitochondria 1.61 1.56 0.89 -1.02 -0.93 -2.61 -0.11 -0.75 1.47 -0.74 -0.53 1.29 -0.08 0.34 -1.04
Mitotic Spindle -1.30 -3.79 -3.55 -1.20 -0.65 -3.64 -1.63 -4.67 -0.67 -2.87 -3.85 -3.22 -5.48 -0.72 -2.28
None -2.07 -7.07 -7.19 -0.77 -2.90 5.65 1.62 -2.59 -11.67 -5.33 5.20 2.17 -2.68 -12.21 -7.29
Nuclear Periphery 2.01 -18.85 -9.03 -11.62 10.47 -0.18 -14.50 -5.39 -6.86 10.68 -0.00 -22.12 -7.88 -10.58 16.14
Nuclear Periphery Foci 0.03 -6.44 -3.32 -2.47 3.83 4.16 -9.41 -9.08 -11.62 6.62 4.16 -12.66 -10.40 -13.13 7.94
Nucleolus 2.26 0.96 3.78 1.68 2.31 -2.51 2.69 3.89 5.92 1.83 -2.15 1.29 5.01 6.55 2.55
Nucleus 4.47 -19.25 -2.92 -8.04 17.72 6.03 -12.04 -10.41 -14.63 7.11 6.50 -22.68 -9.73 -14.71 16.88
Peroxisomes -1.95 4.11 4.10 2.50 0.01 -4.22 3.01 -0.85 2.10 -1.10 -4.45 5.22 -0.26 3.35 -1.09
Vacuole -0.04 -7.93 -14.01 -11.39 -6.56 -15.62 -17.40 -19.92 -10.35 7.80 -13.24 -22.35 -25.68 -16.79 3.18
Vacuole Periphery 2.49 2.70 0.41 -1.02 -1.14 -4.07 6.30 -0.34 0.76 -0.99 -3.09 5.45 -0.39 0.63 -1.28
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YGR111W

YGR111W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr111w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available