Standard name
Human Ortholog
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.05 0.09 0.05 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.14 0.13 0.14 0.08 0.23 0.22 0.19 0.23 0.31 0.36 0.38 0 0 0 0 0.09 0.1 0 0 0 0 0 0.05
Nucleus 0 0.07 0 0 0.05 0 0 0 0 0.05 0.05 0 0.05 0 0 0.12 0.1 0.05 0 0 0 0 0 0
Nuclear Periphery 0.84 0.75 0.85 0.82 0.72 0.72 0.66 0.61 0.7 0.59 0.61 0.58 0.85 0.84 0.77 0.64 0.65 0.68 0.89 0.75 0.9 0.89 0.84 0.79
Nucleolus 0.18 0.14 0.26 0.22 0.26 0.34 0.39 0.41 0.41 0.41 0.42 0.45 0.11 0.09 0.07 0.13 0.09 0.19 0 0.06 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.14 0.07 0.07 0.09 0.06 0 0 0.06 0 0 0 0.07 0.13 0.13 0.23 0.25 0.22 0 0.11 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 0 0 0 1 1 0 0 0 0 2 1 1 0 0 0 0 3 1 4
Bud 0 0 0 0 0 0 0 2 0 1 3 0 0 0 0 0 0 1 1 2 1 4 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 1 0 0 0 0 2 1 0 0 0 2 0 2 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 0 0 1 0 0 0 0 0 0
Cytoplasm 0 0 9 11 22 12 10 12 6 6 5 5 1 15 22 9 11 7 0 0 1 1 0 1
Endoplasmic Reticulum 0 0 1 0 1 1 3 2 3 5 1 0 5 2 3 8 9 5 0 0 0 0 1 3
Endosome 1 0 4 1 9 0 6 8 3 0 1 2 3 1 1 5 4 7 0 0 4 3 3 15
Golgi 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 2 2
Mitochondria 7 8 30 35 25 78 83 111 82 131 134 129 2 0 0 8 19 16 1 0 0 7 18 23
Nucleus 5 4 7 11 14 5 17 25 12 19 20 11 6 12 6 23 23 9 2 1 1 0 5 4
Nuclear Periphery 110 42 193 209 219 247 252 352 249 246 228 196 102 238 184 125 144 114 156 48 207 339 404 395
Nucleolus 24 8 59 56 79 117 147 233 147 172 158 151 13 26 17 25 20 32 6 4 2 12 12 13
Peroxisomes 0 0 1 0 1 0 1 1 1 0 0 1 0 0 2 0 0 1 0 0 0 0 1 2
SpindlePole 0 1 2 0 1 0 3 0 1 1 0 1 0 6 9 0 4 1 0 0 2 2 3 2
Vac/Vac Membrane 14 8 16 17 29 19 13 23 22 12 13 10 8 37 30 44 54 37 6 7 2 4 18 24
Unique Cell Count 131 56 226 254 306 344 380 575 357 417 372 337 120 284 238 195 220 167 177 65 230 383 481 500
Labelled Cell Count 161 72 323 341 404 479 538 770 527 594 563 508 140 339 281 248 289 233 177 65 230 383 481 500


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 13.4 12.3 12.4 12.5 10.5 10.4 10.6 10.7 10.0 9.9 9.8 15.7 14.4 15.1 10.6 12.0 15.7 16.5 16.0 17.3
Std Deviation (1e-4) 3.2 4.1 4.0 3.6 3.7 2.9 3.6 3.3 3.6 4.3 3.7 3.0 6.3 4.8 6.2 6.4 7.1 9.0 5.6 4.8 5.9
Intensity Change (Log2) – – – 0.02 0.02 -0.22 -0.24 -0.21 -0.19 -0.29 -0.3 -0.33 0.36 0.23 0.3 -0.21 -0.03 0.36 0.43 0.38 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.6 -0.3 -0.9 -1.5 -1.7 -2.0 -2.1 -1.9 0 0.7 2.3 0.3 0.5 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 -1.9 2.8 2.6 2.0 2.9 5.1 6.0 6.5 -3.5 -6.3 -5.8 -3.3 -1.6 -1.1
Nucleus 0.7 0.9 0 0.8 0.8 0.2 0.9 1.3 0.1 0 0.7 -0.4 3.5 3.1 1.1
Nuclear Periphery -0.9 -3.8 -3.8 -5.1 -6.6 -4.3 -6.9 -6.3 -6.9 -0.1 -0.5 -2.2 -5.1 -4.9 -4.1
Nucleolus -1.0 -0.1 2.0 3.2 3.8 3.7 3.8 4.0 4.5 -3.3 -5.1 -5.5 -3.4 -4.7 -1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0
Vacuole -0.2 1.0 -0.8 -2.0 -1.8 -0.4 -2.5 -2.0 -2.3 -0.1 2.2 2.0 4.5 5.1 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.6993 13.9608 13.1094 11.8437 12.254 12.4996 10.6253 16.1353 14.5771 13.5446 12.42 13.7767 11.2872 15.7136 12.3259 12.201 13.2978 11.7674
Actin 0.0142 0.0001 0.0003 0.0002 0.0077 0.0001 0.0123 0.0001 0.0008 0.0051 0.0201 0 0.1224 0.0001 0.002 0.0024 0.0132 0.0001
Bud 0.0003 0 0 0 0 0 0.0002 0 0 0.0003 0.0006 0 0.0008 0 0.0011 0.0001 0.0003 0.0001
Bud Neck 0.0072 0 0 0 0.0002 0 0.0095 0 0 0.0001 0.0006 0 0.0101 0 0.0002 0.0001 0.0001 0.0006
Bud Periphery 0.0005 0 0 0.0001 0.0001 0 0.0002 0.0001 0.0003 0.0011 0.0007 0 0.0026 0.0002 0.0013 0.0007 0.0023 0.0001
Bud Site 0.0062 0 0.0001 0 0.0002 0 0.0009 0 0 0.0002 0.0037 0 0.0115 0 0.0015 0.0001 0.0005 0
Cell Periphery 0.0002 0 0 0 0 0 0.0001 0 0.0001 0.0002 0.0002 0 0.0473 0 0.0003 0.0001 0.056 0
Cytoplasm 0.0025 0.0027 0.002 0 0.0007 0 0.0015 0 0 0.0009 0.0042 0 0.0006 0 0.0132 0.0001 0.0005 0.0015
Cytoplasmic Foci 0.0064 0.0001 0.0008 0 0.0018 0.0001 0.0127 0 0.0005 0.0006 0.0092 0 0.0046 0 0.0062 0.0002 0.0132 0.0001
Eisosomes 0.0004 0 0 0 0.0001 0 0.0001 0 0.0001 0.0002 0.0001 0 0.001 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0043 0.0004 0.0004 0.0006 0.0012 0.0001 0.0016 0.0003 0.0004 0.0032 0.0043 0.0001 0.0018 0.0003 0.0012 0.001 0.0014 0.0001
Endosome 0.0083 0.0002 0.0006 0.0001 0.0088 0.0001 0.0089 0.0001 0.0003 0.01 0.0259 0 0.0039 0.0001 0.0014 0.0003 0.0188 0.0001
Golgi 0.0026 0 0.0001 0.0001 0.0019 0.0001 0.0047 0.0001 0.0006 0.0011 0.0135 0 0.0059 0.0001 0.0021 0.0008 0.015 0.0001
Lipid Particles 0.0066 0.0003 0.0005 0.0008 0.004 0.0059 0.0056 0.0015 0.0191 0.0031 0.0084 0.0004 0.0075 0.0006 0.0082 0.0186 0.0984 0.0008
Mitochondria 0.0034 0.0008 0.0005 0.0007 0.0017 0.0043 0.0008 0.0034 0.0022 0.0116 0.0043 0.0001 0.007 0.0012 0.0153 0.0026 0.0059 0.0002
None 0.0049 0.0002 0.007 0.0001 0.0026 0 0.0009 0.0001 0.0003 0.0025 0.0006 0 0.003 0.0001 0.0006 0.0005 0.0008 0.0005
Nuclear Periphery 0.8767 0.9432 0.9508 0.9646 0.9192 0.968 0.8911 0.9181 0.9126 0.859 0.8617 0.981 0.7082 0.94 0.8593 0.9102 0.6447 0.9526
Nucleolus 0.0095 0.0006 0.0004 0.0003 0.0013 0.0035 0.0007 0.0025 0.0014 0.0116 0.0004 0.0001 0.0014 0.0003 0.0009 0.0016 0.001 0.0013
Nucleus 0.011 0.024 0.004 0.003 0.0093 0.0047 0.0015 0.0191 0.0031 0.0185 0.0011 0.0007 0.0043 0.002 0.0049 0.0041 0.0016 0.0223
Peroxisomes 0.002 0 0.0001 0 0.0008 0.0002 0.0013 0 0.0003 0.0004 0.002 0 0.0053 0 0.0101 0.0002 0.0684 0
Punctate Nuclear 0.0122 0.0004 0.0021 0.0004 0.0153 0.0006 0.0091 0.0004 0.0007 0.004 0.0014 0.0002 0.0026 0.0002 0.0016 0.0019 0.0021 0.0025
Vacuole 0.001 0.0008 0.0002 0.0002 0.0004 0.0002 0.0005 0.0004 0.0006 0.0017 0.0009 0.0001 0.0091 0.0004 0.0012 0.0006 0.0221 0.0001
Vacuole Periphery 0.0196 0.0261 0.0301 0.0287 0.0224 0.0119 0.0356 0.0537 0.0566 0.0647 0.0359 0.0172 0.039 0.0545 0.0674 0.0537 0.0335 0.017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.2872 43.6772 34.2109 46.2429 49.7107 24.4161 45.8487 45.3305 43.4346 44.3396
Translational Efficiency 2.0706 1.9969 1.9289 1.2892 1.5011 2.9953 1.5054 1.6149 1.5998 1.582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
Localization
Cell Percentages nuclear periphery (89%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup57

Nup57


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup57-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available