ORF
Human Ortholog
Description Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.68 0.61 0.7 0.72 0.62 0.6 0.55 0.49 0.49 – 0.44 0.49 0.44 0.2 0.31 – – – 0.2 0.31 0.22 0.33 0.27 0.23
Bud 0.05 0.07 0.12 0.08 0.13 0.09 0.12 0.11 0.08 – 0.08 0.07 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.63 0.65 0.61 0.67 0.43 0.45 0.4 0.33 0.42 – 0.35 0.41 0.6 0.68 0.66 – – – 0.74 0.57 0.67 0.58 0.44 0.29
Cytoplasm 0 0.05 0.08 0.12 0.09 0.15 0.15 0.12 0.08 – 0.11 0.08 0.34 0.43 0.27 – – – 0 0 0 0 0.1 0.24
Endoplasmic Reticulum 0 0.16 0 0 0.13 0.09 0.09 0.12 0.07 – 0.08 0.09 0.09 0.09 0.15 – – – 0 0.06 0 0 0.13 0.17
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0.13 0 0.13 0 0.06 0.23 0 – 0 0.11 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.59 0.2 0.07 0.19 0.09 0.28 0.33 0.2 0.59 – 0.63 0.48 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.09 0 0.06 0.06 0 0.05 0 0 0.05 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 89 127 287 143 186 205 198 188 172 – 128 156 101 73 106 – – – 29 68 103 38 89 66
Bud 6 14 50 15 38 30 44 43 29 – 23 21 3 5 7 – – – 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 4 0 3 2
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 82 135 249 132 128 154 144 128 147 – 103 131 140 248 225 – – – 107 127 314 68 144 83
Cytoplasm 3 10 34 23 27 50 53 45 28 – 31 25 80 157 94 – – – 1 1 3 1 31 70
Endoplasmic Reticulum 4 33 12 7 40 30 34 45 23 – 23 30 22 32 52 – – – 3 12 10 3 42 50
Endosome 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 1 3 0 0 1
Golgi 4 9 52 7 39 8 22 90 2 – 8 36 2 1 1 – – – 1 4 3 0 7 5
Mitochondria 77 42 30 37 26 96 120 79 204 – 184 154 4 0 1 – – – 0 1 14 2 4 1
Nucleus 0 0 2 0 2 1 0 3 1 – 0 1 0 0 2 – – – 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 2 0 – 1 2 0 0 0 – – – 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 12 5 24 12 9 18 14 9 18 – 0 7 5 0 2 – – – 0 0 2 0 0 0
SpindlePole 0 0 6 1 2 0 1 3 1 – 0 2 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 1 3 1 0 3 0 8 1 – 1 3 0 0 0 – – – 0 1 3 0 0 0
Unique Cell Count 130 208 411 198 298 343 361 386 348 292 319 232 365 342 145 223 470 118 328 290
Labelled Cell Count 277 377 750 378 497 595 630 643 626 502 568 357 516 490 145 223 470 118 328 290


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.1 13.7 10.2 9.5 11.1 9.5 10.5 11.1 9.3 – 9.5 11.1 14.7 19.2 18.9 – – – 10.9 12.9 14.2
Std Deviation (1e-4) 1.9 3.1 1.6 1.4 1.6 1.7 1.6 2.0 2.1 – 2.4 2.7 2.4 4.4 5.1 – – – 2.2 2.3 2.6
Intensity Change (Log2) – – – -0.1 0.12 -0.1 0.04 0.12 -0.13 – -0.11 0.13 0.53 0.91 0.89 – – – 0.1 0.34 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -2.1 -2.9 -4.3 -6.1 -5.7 – -6.9 -5.7 -6.5 -13.9 -10.6 – – –
Bud -1.7 0.2 -1.5 0 -0.5 -1.7 – -1.8 -2.5 -4.8 -5.8 -5.2 – – –
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Cell Periphery 1.5 -4.6 -4.3 -5.7 -7.7 -5.0 – -6.6 -5.2 -0.1 2.1 1.5 – – –
Cytoplasm 1.3 0.4 2.7 2.8 1.6 -0.1 – 1.1 -0.2 8.4 11.2 7.0 – – –
Endoplasmic Reticulum 0.4 5.3 3.5 3.8 4.8 2.4 – 3.0 3.7 3.6 3.5 6.0 – – –
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Golgi -3.6 0.2 -5.2 -3.1 3.9 -6.4 – -4.6 -0.6 -5.2 -6.8 -6.6 – – –
Mitochondria 4.2 0.7 7.6 9.1 5.4 15.3 – 15.8 12.6 -3.0 -5.3 -4.8 – – –
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Peroxisomes 0.1 -1.8 -0.4 -1.3 -2.5 -0.4 – -4.2 -2.4 -2.2 -4.7 -3.9 – – –
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Vacuole 0 0 0 0 1.6 0 – 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.5907 17.2879 14.5766 13.4583 9.8424 14.0388 13.8493 17.24 15.0858 14.9492 12.621 16.0362 13.3364 15.3022 13.6455 12.9274 12.6583 13.0282
Actin 0.1217 0.0151 0.0368 0.0031 0.2426 0.0415 0.0651 0.0353 0.0576 0.0369 0.1068 0.0322 0.1546 0.0504 0.1058 0.0665 0.1331 0.0692
Bud 0.0004 0.0003 0.0003 0.0001 0.0005 0.0004 0.0007 0.0001 0.0006 0.0004 0.0021 0.0002 0.0008 0.0003 0.0011 0.0008 0.0004 0.0004
Bud Neck 0.0051 0.0032 0.0061 0.0029 0.0118 0.0033 0.0027 0.003 0.0034 0.0047 0.0118 0.0012 0.0041 0.0086 0.0127 0.0206 0.0077 0.0042
Bud Periphery 0.0017 0.0008 0.0016 0.0016 0.0027 0.0048 0.0041 0.0007 0.0027 0.0034 0.0049 0.0017 0.0025 0.0014 0.0039 0.0057 0.0027 0.0027
Bud Site 0.0075 0.1013 0.0192 0.0031 0.002 0.0012 0.0114 0.0175 0.0081 0.0041 0.0256 0.0007 0.0103 0.0474 0.0358 0.0172 0.0034 0.004
Cell Periphery 0.0537 0.2105 0.2387 0.2755 0.1816 0.2138 0.1349 0.1356 0.1816 0.2499 0.1197 0.1832 0.0632 0.1294 0.1612 0.3247 0.1067 0.1465
Cytoplasm 0.0037 0.0722 0.0048 0.0031 0.0013 0.0019 0.0107 0.0026 0.0099 0.0064 0.0042 0.0017 0.0052 0.0064 0.0096 0.0112 0.0057 0.0101
Cytoplasmic Foci 0.0066 0.0016 0.0004 0.0001 0.0041 0.014 0.0057 0.0001 0.0067 0.0056 0.0142 0.0005 0.0218 0.0002 0.0033 0.0142 0.0042 0.0015
Eisosomes 0.7344 0.5826 0.6731 0.7031 0.3463 0.6681 0.7024 0.7987 0.6844 0.6271 0.6139 0.7516 0.6916 0.7523 0.6354 0.4787 0.6817 0.7392
Endoplasmic Reticulum 0.0015 0.0029 0.0008 0.0032 0.0013 0.0021 0.0061 0.0034 0.005 0.0181 0.0042 0.0038 0.0047 0.0013 0.0035 0.0089 0.0036 0.0027
Endosome 0.006 0.0012 0.0001 0 0.0059 0.0037 0.007 0 0.0126 0.0028 0.0223 0.0014 0.0081 0 0.0029 0.0134 0.0073 0.0007
Golgi 0.0051 0.0006 0.0007 0.0003 0.0069 0.0048 0.0059 0.001 0.0046 0.0097 0.0238 0.0025 0.0068 0.0006 0.0042 0.0082 0.0047 0.0016
Lipid Particles 0.0142 0.0003 0.0005 0.0001 0.0406 0.0084 0.0122 0.0001 0.004 0.0053 0.0123 0.0007 0.0058 0.0002 0.0043 0.0069 0.0082 0.0005
Mitochondria 0.0154 0.0012 0.0137 0.0018 0.1386 0.005 0.0107 0.0002 0.0029 0.0158 0.014 0.0159 0.0029 0.0002 0.0096 0.0021 0.0116 0.0127
None 0.0137 0.0038 0.0006 0.0009 0.001 0.008 0.0039 0.0004 0.0009 0.0005 0.0016 0.001 0.0034 0.0004 0.0012 0.006 0.0051 0.0007
Nuclear Periphery 0.0017 0.0005 0.0002 0.0003 0.0008 0.0004 0.0017 0.0007 0.0033 0.0015 0.0055 0.0005 0.0016 0.0004 0.0009 0.0037 0.004 0.0008
Nucleolus 0.0003 0.0001 0.0001 0 0.0003 0.0002 0.0014 0 0.0004 0.0001 0.0005 0 0.0002 0 0.0001 0.0006 0.0003 0
Nucleus 0.0003 0.0004 0.0001 0 0.0002 0.0001 0.0009 0 0.0054 0.0001 0.0008 0.0001 0.0004 0.0001 0.0002 0.0011 0.0004 0.0004
Peroxisomes 0.0036 0.0002 0.0015 0.0002 0.0078 0.016 0.0076 0.0001 0.0024 0.0068 0.0071 0.0003 0.0058 0.0001 0.0026 0.0043 0.002 0.0005
Punctate Nuclear 0.0026 0.0001 0 0 0.0007 0.0008 0.0035 0 0.0022 0.0001 0.0023 0.0001 0.005 0 0.0004 0.0034 0.0067 0.0012
Vacuole 0.0005 0.001 0.0007 0.0004 0.0015 0.0013 0.0011 0.0003 0.0008 0.0007 0.0015 0.0005 0.001 0.0003 0.0009 0.0017 0.0003 0.0003
Vacuole Periphery 0.0003 0.0001 0 0 0.0016 0.0002 0.0003 0 0.0003 0.0001 0.0008 0.0001 0.0002 0 0.0002 0.0004 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.6089 20.9268 24.4874 44.2066 28.6006 39.1444 36.1128 45.5891 54.6188 35.4943
Translational Efficiency 2.2054 2.1371 2.6099 2.2673 2.1852 1.7884 1.5023 2.0978 1.8926 1.8986

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✔
ORF
Human Ortholog
Description Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
Localization
Cell Percentages cell periphery (28%), actin (10%), mixed (36%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YGR130C

YGR130C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr130c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available