Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.05 0.05 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 – – – 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0.06 0.09 0 0 0 – – – 0 0.06 0 0 0.07 0.05
Cytoplasm 0.95 0.94 0.97 0.97 0.93 0.84 0.81 0.72 – 0.68 0.61 0.6 0.99 0.99 0.99 – – – 0.71 0.64 0.65 0.64 0.62 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.11 0.1 0.32 0.35 0.42 – 0.46 0.51 0.48 0 0 0 – – – 0.1 0.09 0.16 0.16 0.11 0.1
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 0 0 0 0 2 3 – 6 12 13 1 0 1 – – – 14 16 12 1 11 7
Bud 2 9 1 2 9 7 12 10 – 18 15 14 0 0 1 – – – 2 7 3 1 4 14
Bud Neck 5 1 3 0 3 0 3 4 – 2 1 0 4 5 2 – – – 5 13 6 0 7 8
Bud Site 0 1 0 0 1 0 0 1 – 1 0 3 0 0 0 – – – – – – – – –
Cell Periphery 9 8 3 3 1 6 5 6 – 12 19 33 6 8 14 – – – 11 21 11 1 16 11
Cytoplasm 270 330 296 196 280 314 358 248 – 244 197 228 519 471 515 – – – 209 227 200 79 145 148
Endoplasmic Reticulum 1 2 2 0 3 0 2 1 – 3 4 8 2 2 0 – – – 3 2 3 1 0 4
Endosome 1 1 0 0 0 0 0 0 – 0 0 1 2 1 0 – – – 3 6 5 2 3 3
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 1 1 0 0 0 0
Mitochondria 3 17 10 23 30 120 152 144 – 165 164 180 0 1 0 – – – 29 32 47 19 26 24
Nucleus 1 0 0 1 0 2 1 3 – 2 1 2 0 0 4 – – – 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 5 1 5 0 0 0 – – – 0 0 0 0 0 1
Nucleolus 1 0 0 1 0 1 1 0 – 1 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 1 – 2 0 2 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 0 3 0 2 3 5 2 – 9 5 10 0 0 0 – – – 0 5 2 2 4 4
Vac/Vac Membrane 1 0 1 0 2 1 7 1 – 1 2 6 0 0 1 – – – 6 7 4 4 6 8
Unique Cell Count 284 350 305 203 302 374 440 346 361 324 377 523 477 519 293 356 309 123 236 244
Labelled Cell Count 299 374 319 226 331 455 548 424 471 421 506 534 488 538 293 356 309 123 236 244


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.7 3.4 3.4 3.3 2.7 2.7 3.1 – 2.9 3.1 3.4 4.9 5.0 5.2 3.9 4.2 4.4 4.9 5.2 5.8
Std Deviation (1e-4) 0.6 0.7 1.2 1.7 1.4 1.3 1.1 1.1 – 1.3 1.6 2.1 0.8 0.9 1.0 0.9 1.0 1.0 1.5 1.4 2.5
Intensity Change (Log2) – – – 0.02 -0.01 -0.3 -0.32 -0.14 – -0.22 -0.11 0.0 0.55 0.56 0.61 0.22 0.32 0.39 0.53 0.62 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6603 0.4536 1.3755 0.4032 0.6245 0.4233 2.7954 2.5679 2.2313 1.7706 1.7243 2.5596 1.1438 1.9446 1.8906 2.2268 2.0059 1.9462
Actin 0.0155 0.0042 0.0222 0.0001 0.0019 0.0019 0.0255 0.0004 0.005 0.0005 0.004 0.007 0.0073 0.0008 0.0005 0.0027 0.0001 0.0005
Bud 0.0011 0.0025 0.0163 0 0.0023 0.0017 0.0025 0.0017 0.0047 0.0004 0.0004 0.001 0.0037 0.0087 0.0034 0.0091 0 0.0002
Bud Neck 0.0073 0.0005 0.0041 0.0001 0.0004 0.0001 0.0067 0.0003 0.0064 0.0261 0.0099 0.0016 0.0024 0.0012 0.0013 0.0212 0 0.0003
Bud Periphery 0.0013 0.0071 0.0123 0.0001 0.0077 0.027 0.009 0.0046 0.011 0.0008 0.0028 0.0056 0.0085 0.0051 0.0059 0.02 0 0.004
Bud Site 0.0701 0.026 0.0674 0.0001 0.0003 0.0001 0.0161 0.0136 0.0157 0.002 0.004 0.0001 0.0125 0.0382 0.0055 0.0185 0 0.0001
Cell Periphery 0.0007 0.002 0.0035 0.0006 0.0001 0.0054 0.0066 0.007 0.0114 0.0022 0.0027 0.0001 0.004 0.0019 0.0122 0.0057 0 0.0048
Cytoplasm 0.0601 0.0699 0.0447 0.0701 0.0053 0.0314 0.0338 0.1018 0.0677 0.0621 0.1055 0.0616 0.0668 0.1062 0.0944 0.1034 0.0325 0.111
Cytoplasmic Foci 0.0244 0.0055 0.0072 0.0052 0.0344 0.0016 0.0152 0.0028 0.0066 0.002 0.0012 0.0029 0.0046 0.0048 0.0022 0.0037 0.0016 0.0022
Eisosomes 0.0003 0.001 0.0006 0.0003 0.0009 0.0004 0.0007 0.0007 0.0002 0.0004 0.0002 0.0001 0.0003 0.0002 0.0003 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0088 0.0047 0.006 0.0011 0.0004 0.0005 0.0034 0.0009 0.0006 0.0004 0.0003 0.0003 0.0008 0.0008 0.001 0.0013 0.0001 0.0004
Endosome 0.0266 0.0028 0.0014 0.0006 0.0063 0.0034 0.0046 0.0007 0.0037 0.0002 0.0001 0.0014 0.0006 0.002 0.0004 0.001 0.0001 0.0002
Golgi 0.0034 0.0008 0.001 0.0001 0.002 0.0088 0.0029 0.0001 0.0013 0.0001 0 0.0006 0.0003 0.0001 0.0001 0.0004 0 0
Lipid Particles 0.0051 0.0023 0.0013 0.0003 0.0055 0.0006 0.0039 0.0002 0.0015 0.0001 0 0.0008 0.0006 0.0002 0.0003 0.0011 0.0001 0.0001
Mitochondria 0.0025 0.0017 0.0013 0.0002 0.0013 0.0028 0.002 0.0005 0.0015 0.0002 0.0001 0.0002 0.0004 0.0003 0.0006 0.0005 0 0.0001
None 0.5405 0.8559 0.7977 0.9171 0.8599 0.9133 0.826 0.8599 0.8552 0.8994 0.8678 0.9135 0.8833 0.8249 0.8688 0.8075 0.9645 0.8752
Nuclear Periphery 0.0611 0.0041 0.0026 0.0004 0.002 0.0002 0.0031 0.0002 0.0009 0.0001 0.0001 0.0013 0.0004 0.0003 0.0002 0.0002 0.0001 0.0001
Nucleolus 0.0095 0.0012 0.0014 0.0003 0.0009 0.0001 0.0016 0.0006 0.0007 0.0001 0.0001 0.0001 0.0004 0.0005 0.0004 0.0002 0.0001 0
Nucleus 0.0689 0.0029 0.0042 0.0005 0.0012 0.0001 0.013 0.0014 0.0008 0.0004 0.0002 0.0002 0.0007 0.0016 0.001 0.0008 0.0001 0.0002
Peroxisomes 0.0027 0.0006 0.0004 0.0001 0.004 0.0002 0.0164 0.0001 0.003 0.0001 0 0.0005 0.0009 0.0001 0.0001 0.0002 0.0001 0.0002
Punctate Nuclear 0.0827 0.0024 0.003 0.0022 0.0624 0.0002 0.0045 0.0014 0.0015 0.0002 0.0001 0.0009 0.001 0.0007 0.0005 0.0003 0.0003 0.0001
Vacuole 0.0056 0.0015 0.0012 0.0004 0.0006 0.0002 0.0022 0.0009 0.0006 0.0022 0.0004 0.0003 0.0006 0.0013 0.0007 0.0017 0.0001 0.0002
Vacuole Periphery 0.0018 0.0005 0.0002 0 0.0003 0.0001 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.5883 10.1578 37.2909 24.2858 5.0401 5.3795 17.568 31.3761 29.1377 9.1571
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
87 1510 139 1423 97 2058 811 1392 184 3568 950 2815

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1104.99 722.13 1376.94 1137.88 640.44 729.91 894.68 1034.35 860.09 726.62 965.24 1086.69
Standard Deviation 287.05 97.25 268.55 392.15 75.75 94.11 183.22 304.55 309.48 95.53 261.27 355.36
Intensity Change Log 2 — -0.613703 0.317432 0.042315 — 0.188655 0.482308 0.691589 — -0.265501 0.380139 0.315594

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.024374 0.000948 0.019105 0.026948 0.000169 0.000529 0.013288 0.028647 0.011614 0.000706 0.014139 0.027788
Bud Neck 0.014534 0.040532 0.031354 0.033540 0.017760 0.060573 0.049950 0.040812 0.016235 0.052092 0.047229 0.037136
Bud Site 0.128907* 0.013122 0.171630* 0.200385* 0.001648 0.024137 0.150667* 0.239278* 0.061819 0.019475 0.153734* 0.219617*
Cell Periphery 0.189459* 0.002188 0.613076* 0.462409* 0.000309 0.002078 0.084208 0.265399* 0.089744 0.002125 0.161590* 0.364989*
Cytoplasm 0.190662* 0.418116* 0.039018 0.153496* 0.304049* 0.425251* 0.266891* 0.244995* 0.250437* 0.422231* 0.233550* 0.198742*
Cytoplasmic Foci 0.037944 0.266525* 0.004833 0.004346 0.224537* 0.212872* 0.019301 0.012958 0.136311 0.235578* 0.017184 0.008605
Eisosomes 0.014644 0.000614 0.005414 0.012357 0.000265 0.000614 0.005071 0.008283 0.007064 0.000614 0.005121 0.010342
Endoplasmic Reticulum 0.040280 0.001330 0.052746 0.021939 0.000773 0.000609 0.034837 0.013473 0.019453 0.000914 0.037457 0.017752
Endosome 0.018970 0.018241 0.003519 0.001146 0.005480 0.018251 0.003129 0.004302 0.011858 0.018246 0.003186 0.002707
Golgi 0.018766 0.004502 0.003978 0.001382 0.003120 0.006041 0.000932 0.003541 0.010518 0.005390 0.001377 0.002450
Lipid Particles 0.016155 0.008550 0.010051 0.004313 0.012550 0.008839 0.003549 0.006222 0.014254 0.008717 0.004501 0.005257
Mitochondria 0.001942 0.008116 0.000363 0.000921 0.017208 0.015024 0.001174 0.005013 0.009990 0.012101 0.001055 0.002945
Mitotic Spindle 0.005504 0.001636 0.000417 0.000681 0.000878 0.004788 0.002846 0.003259 0.003065 0.003454 0.002490 0.001956
None 0.000054 0.002830 0.000044 0.000418 0.004773 0.002308 0.001436 0.000496 0.002542 0.002529 0.001232 0.000456
Nuclear Periphery 0.000323 0.000304 0.000289 0.000852 0.000817 0.000315 0.000765 0.000494 0.000584 0.000311 0.000696 0.000675
Nuclear Periphery Foci 0.003494 0.000526 0.002007 0.000996 0.000333 0.000169 0.002157 0.001188 0.001828 0.000320 0.002135 0.001091
Nucleolus 0.000083 0.002126 0.000020 0.000041 0.002143 0.001582 0.000183 0.000082 0.001169 0.001812 0.000159 0.000061
Nucleus 0.040765 0.126085 0.003289 0.019033 0.358128* 0.142003* 0.249152* 0.036750 0.208070* 0.135267* 0.213179* 0.027794
Peroxisomes 0.008187 0.017914 0.000479 0.001022 0.012764 0.015501 0.000797 0.001769 0.010600 0.016522 0.000751 0.001391
Vacuole 0.236202* 0.064262 0.037559 0.053196 0.031077 0.056738 0.109162 0.080646 0.128066 0.059922 0.098685 0.066770
Vacuole Periphery 0.008750 0.001536 0.000810 0.000579 0.001219 0.001776 0.000505 0.002392 0.004780 0.001675 0.000549 0.001476

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.64 0.70 -0.67 -14.38 -2.10 -3.66 -9.66 -15.58 -15.35 -7.42 4.29 -0.51 -5.70 -21.03 -8.64
Bud Neck -8.56 -4.23 -7.06 2.54 -0.24 -5.84 -3.34 -2.83 6.27 1.29 -8.64 -5.30 -4.85 6.91 1.84
Bud Site 5.80 -1.74 -3.67 -26.11* -1.09 -11.44 -15.69 -28.87* -25.16* -8.64 3.94 -5.95 -13.36 -35.85* -8.36
Cell Periphery 6.29 -10.81 -8.95 -43.99* 5.55 -2.71 -10.85 -26.50 -26.24* -15.80 5.58 -3.62 -15.86 -48.45* -16.86
Cytoplasm -11.74 7.64 1.36 28.24 -14.36 -4.81 1.36 1.60 18.09 0.51 -10.25 0.91 2.21 32.75 2.31
Cytoplasmic Foci -25.04 4.41 4.63 43.91 0.01 0.37 8.78 9.00 41.70 2.37 -6.83 8.18 8.75 60.41 4.57
Eisosomes 4.27 2.58 0.59 -12.66 -4.88 -3.65 -8.10 -11.45 -10.85 -4.32 3.93 1.48 -1.88 -16.55 -7.81
Endoplasmic Reticulum 2.58 -0.80 1.17 -8.84 2.65 1.28 -10.33 -7.41 -7.67 5.66 2.57 -2.11 0.23 -11.66 5.24
Endosome -0.05 2.57 3.06 15.19 2.23 -9.65 2.64 1.48 11.35 -1.24 -2.47 3.06 3.29 18.51 0.86
Golgi 1.71 1.73 2.11 5.19 1.62 -1.41 1.03 -0.11 2.95 -3.21 1.17 2.25 1.98 5.32 -1.97
Lipid Particles 1.89 0.93 3.04 4.62 1.20 1.47 3.76 2.59 2.53 -2.16 2.39 4.14 4.02 4.89 -0.48
Mitochondria -4.33 1.39 0.87 7.26 -3.11 0.11 1.50 1.14 5.54 -2.58 -0.49 1.57 1.24 8.51 -2.40
Mitotic Spindle 1.00 1.40 1.17 0.96 -1.70 -3.74 -2.02 -2.90 0.54 -0.43 -0.36 -0.06 0.16 1.18 0.35
None -5.19 1.11 -2.40 4.23 -2.75 1.28 1.76 2.33 4.71 3.06 -0.09 1.23 2.08 6.27 2.79
Nuclear Periphery 0.23 0.45 -3.49 -4.37 -4.42 3.59 -0.60 1.96 -1.69 3.58 3.31 -2.35 -0.17 -4.24 2.44
Nuclear Periphery Foci 4.82 2.06 4.15 -2.85 2.63 2.18 -4.39 -3.54 -4.78 2.43 4.86 -1.02 2.30 -6.03 3.09
Nucleolus -4.87 1.95 1.36 5.00 -4.68 0.93 4.28 4.63 6.00 2.55 -2.20 3.89 4.51 7.66 2.94
Nucleus -4.03 2.79 1.63 23.00 -8.82 7.09 2.32 10.20 19.04 23.02 3.65 -1.26 8.36 29.80 22.66
Peroxisomes -1.63 1.18 1.10 13.76 -2.45 -0.92 3.47 3.20 12.03 -2.90 -1.84 2.79 2.62 18.07 -3.31
Vacuole 6.22 6.96 6.07 -0.83 -4.00 -1.43 -8.01 -6.25 -10.17 3.00 4.78 1.17 3.00 -8.48 5.00
Vacuole Periphery 1.18 1.31 1.36 4.82 1.11 -1.11 1.33 -1.29 -0.66 -2.86 1.05 1.49 1.16 0.97 -2.65
Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (35%), cell periphery (26%), mixed (0%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tpo2

Tpo2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpo2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available