Standard name
Human Ortholog
Description Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0.06 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0.05 0 0.05 0.07 0 0.06 – 0.11 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0.05 – 0 0 0.09 0.14 0.17 0.07 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.11 0 0 0.08 0 0 0 0 – 0.06 0 0.21 0.14 0.14 0.19 0.28 0.18 0.09 0.11 0.13 0.15 0.12 0.12
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.24 0.25 0.29 0.09 0.23 0.23 0.22 0.38 – 0.32 0.38 0.08 0.06 0.19 0.09 0 0.11 0 0 0 0 0 0
Nucleus 0.07 0.09 0.1 0.13 0.08 0.07 0.07 0.08 0.08 – 0.06 0.07 0.1 0.13 0.1 0.2 0.14 0.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.74 0.71 0.48 0.44 0.52 0.43 0.47 0.45 0.47 – 0.46 0.49 0.71 0.56 0.53 0.6 0.71 0.62 0 0.1 0.05 0.08 0.07 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.81 0.42 0.68 0.62 0.76 0.66 0.65 0.65 0.6 – 0.56 0.64 0.5 0.45 0.36 0.33 0.44 0.39 0.74 0.67 0.7 0.57 0.59 0.62
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 2 1 4 6 9 15 28 17 – 15 10 0 0 2 2 2 0 0 1 1 0 4 3
Bud Neck 0 0 6 5 4 6 9 10 3 – 5 4 2 0 0 0 0 0 0 0 2 2 2 3
Bud Site 0 1 8 16 6 16 34 16 18 – 35 34 0 0 4 1 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 2 5 8 9 6 12 19 15 14 – 12 4 18 40 56 22 16 29 0 0 1 1 1 1
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 – 1 1 0 1 0 2 3 7 0 0 0 0 1 0
Endosome 23 21 5 3 24 7 20 7 2 – 17 8 43 38 46 64 95 70 12 21 37 25 27 45
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 2 1 1 0 4
Mitochondria 5 45 70 87 27 75 120 115 116 – 98 138 17 17 62 29 12 43 0 3 4 5 5 11
Nucleus 9 17 28 38 25 23 37 45 23 – 18 24 21 36 32 68 47 78 0 8 9 2 8 7
Nuclear Periphery 1 5 1 1 0 0 1 1 0 – 4 0 2 0 2 1 4 1 0 0 0 0 0 0
Nucleolus 90 134 132 130 163 138 241 239 143 – 140 179 144 156 173 201 239 236 5 21 16 13 16 20
Peroxisomes 3 3 4 7 10 7 19 13 7 – 14 11 5 4 7 5 6 4 1 0 1 3 3 7
SpindlePole 98 80 188 184 238 213 337 345 182 – 170 232 102 127 116 110 149 148 96 136 208 98 136 230
Vac/Vac Membrane 0 2 0 1 4 1 1 2 0 – 2 1 2 4 8 7 6 13 1 3 4 2 7 8
Unique Cell Count 121 189 275 298 314 321 515 530 303 306 365 202 281 324 333 337 383 130 203 299 173 230 369
Labelled Cell Count 231 315 452 485 513 508 853 836 525 532 646 356 423 508 512 581 632 130 203 299 173 230 369


Microtubule, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.8 4.1 3.9 4.3 3.7 3.7 3.7 3.4 – 3.7 3.1 5.2 5.1 4.6 7.2 8.4 8.0 5.4 4.9 5.5
Std Deviation (1e-4) 0.8 0.9 1.2 1.1 1.0 0.9 1.3 1.1 1.2 – 2.9 0.9 1.2 1.4 1.5 1.6 2.0 2.0 1.5 1.4 1.5
Intensity Change (Log2) – – – -0.06 0.06 -0.16 -0.15 -0.14 -0.26 – -0.15 -0.41 0.34 0.31 0.15 0.8 1.04 0.96 0.38 0.26 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0343 2.2145 2.0832 2.0862 1.9172 2.107 2.4428 2.7884 3.057 2.765 2.738 2.7677 2.6407 3.146 3.0701 3.2821 2.3454 2.6937
Actin 0.0035 0 0.0255 0.0001 0.0824 0.0034 0.0124 0.0002 0.0012 0.0109 0.0223 0.0029 0.0003 0 0.0016 0.0001 0.0002 0.0001
Bud 0.0001 0 0 0 0.0002 0.0001 0.0002 0 0.0001 0.0007 0.0003 0 0 0 0 0 0 0
Bud Neck 0.0016 0.0006 0.0008 0.0003 0.0022 0.0015 0.0007 0.0004 0.0005 0.0003 0.001 0.0007 0.0004 0.0004 0.0002 0.0002 0.0004 0.0005
Bud Periphery 0.0004 0 0 0 0.0004 0.0002 0.0003 0 0.0002 0.001 0.0005 0.0001 0 0.0001 0.0001 0 0.0001 0
Bud Site 0.0012 0.0003 0.0003 0.0001 0.0007 0.0002 0.0007 0.0004 0.0022 0.0032 0.0014 0.0001 0.0001 0.0018 0.0001 0.0002 0.0001 0
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0
Cytoplasm 0.0023 0.0001 0.0002 0.0001 0.0004 0.0001 0.0009 0.0002 0.0026 0.0024 0.0008 0.0003 0.0001 0.0015 0 0.0002 0.0001 0
Cytoplasmic Foci 0.0072 0.0027 0.0021 0.0104 0.0065 0.002 0.0155 0.0101 0.0135 0.0062 0.0075 0.0036 0.0096 0.0017 0.0006 0.0016 0.0028 0.0007
Eisosomes 0.0001 0 0.0001 0 0.0005 0.0001 0.0001 0 0 0.0001 0.0003 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0013 0 0.0001 0 0.0017 0.0004 0.0018 0 0.0007 0.0076 0.0006 0.0002 0 0.0001 0.0002 0 0 0
Endosome 0.0099 0.0001 0.0004 0.0006 0.0075 0.0016 0.0246 0.0008 0.0097 0.0175 0.0073 0.0031 0.0008 0.0003 0.0005 0.0008 0.0009 0.0002
Golgi 0.0028 0.0001 0.0013 0.0007 0.009 0.0011 0.0062 0.0005 0.0014 0.0071 0.0136 0.0018 0.0005 0.0001 0.0003 0.0003 0.0007 0.0002
Lipid Particles 0.0133 0.001 0.0018 0.0085 0.0532 0.0068 0.0214 0.0028 0.0039 0.0086 0.027 0.0064 0.003 0.0033 0.0013 0.0018 0.0123 0.0013
Mitochondria 0.003 0 0.0002 0 0.0066 0.0011 0.0035 0.0001 0.0002 0.0076 0.0181 0.0003 0 0.0002 0.0005 0 0.001 0
None 0.005 0 0.0002 0.0001 0.0005 0.0003 0.0015 0.0001 0.0007 0.0007 0.0004 0.0002 0.0002 0.0053 0.0001 0.0002 0.0003 0
Nuclear Periphery 0.022 0.0008 0.0008 0.0029 0.0094 0.0095 0.0124 0.0007 0.0075 0.0091 0.0094 0.0089 0.0014 0.0025 0.0031 0.0116 0.013 0.0017
Nucleolus 0.0651 0.1187 0.0638 0.0138 0.069 0.075 0.0403 0.064 0.055 0.0466 0.0419 0.0607 0.0293 0.0633 0.0447 0.0433 0.0334 0.0485
Nucleus 0.042 0.1637 0.0384 0.014 0.0058 0.0392 0.0278 0.1536 0.0523 0.0644 0.0169 0.0703 0.0109 0.1196 0.0525 0.0127 0.0067 0.0264
Peroxisomes 0.007 0.0001 0.005 0.0032 0.0171 0.0013 0.0092 0.003 0.0025 0.0024 0.0026 0.0022 0.0034 0.0001 0.0003 0.0002 0.001 0.0001
Punctate Nuclear 0.8095 0.7117 0.8591 0.945 0.7262 0.856 0.8184 0.763 0.8447 0.8006 0.8258 0.8381 0.9399 0.7989 0.8937 0.9267 0.9258 0.9202
Vacuole 0.0014 0 0 0 0.0003 0.0001 0.0012 0.0001 0.0008 0.0017 0.0015 0.0001 0 0.0005 0 0 0.0004 0
Vacuole Periphery 0.0013 0 0 0 0.0004 0.0003 0.0009 0 0.0002 0.0011 0.0008 0.0001 0 0.0001 0.0001 0.0001 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.9029 13.399 3.3142 7.0265 19.1845 18.8216 13.7215 7.2682 5.2625 18.5325
Translational Efficiency 0.7173 0.8848 0.8976 0.5365 0.5865 0.7862 0.717 0.7016 0.7532 0.5398

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
99 1071 220 1337 1798 1966 1664 177 1897 3037 1884 1514

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 674.29 724.26 1077.40 892.76 720.55 790.99 906.82 1042.05 718.14 767.46 926.74 910.21
Standard Deviation 73.72 101.15 115.15 139.97 87.01 97.61 127.43 147.65 86.98 103.89 137.45 148.83
Intensity Change Log 2 — 0.103138 0.676113 0.404903 — 0.134561 0.331718 0.532254 — 0.119456 0.508472 0.472092

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000266 0.000971 0.001073 0.000660 0.000322 0.000380 0.000260 0.000227 0.000319 0.000588 0.000355 0.000609
Bud Neck 0.007171 0.003428 0.003087 0.009263 0.003228 0.005163 0.002540 0.001966 0.003434 0.004551 0.002604 0.008410
Bud Site 0.002534 0.003437 0.003330 0.010221 0.001751 0.002291 0.001131 0.002031 0.001792 0.002695 0.001388 0.009264
Cell Periphery 0.000125 0.000218 0.000110 0.000241 0.000171 0.000086 0.000135 0.000022 0.000168 0.000133 0.000132 0.000216
Cytoplasm 0.054093 0.015674 0.004845 0.037548 0.026541 0.012255 0.022276 0.000267 0.027979 0.013461 0.020241 0.033189
Cytoplasmic Foci 0.150302 0.030823 0.002164 0.006820 0.051721 0.021741 0.003202 0.004454 0.056866 0.024943 0.003080 0.006544
Eisosomes 0.000131 0.000107 0.000052 0.000037 0.000105 0.000076 0.000035 0.000007 0.000106 0.000087 0.000037 0.000034
Endoplasmic Reticulum 0.001438 0.003208 0.004584 0.001723 0.001543 0.001622 0.000944 0.000324 0.001538 0.002181 0.001369 0.001560
Endosome 0.010173 0.005194 0.002118 0.009528 0.005556 0.009215 0.000976 0.000264 0.005797 0.007797 0.001109 0.008445
Golgi 0.007534 0.010637 0.001356 0.005078 0.008090 0.005264 0.000686 0.000272 0.008061 0.007159 0.000764 0.004516
Lipid Particles 0.019978 0.018718 0.002974 0.001495 0.017007 0.014043 0.000952 0.000181 0.017162 0.015692 0.001189 0.001341
Mitochondria 0.004428 0.006609 0.000310 0.007947 0.002249 0.005988 0.000686 0.000384 0.002362 0.006207 0.000642 0.007063
Mitotic Spindle 0.252577* 0.464848* 0.785238* 0.647749* 0.401270* 0.526860* 0.639356* 0.944299* 0.393510* 0.504992* 0.656391* 0.682419*
None 0.013088 0.017470 0.001604 0.012321 0.019137 0.004681 0.019499 0.000290 0.018822 0.009191 0.017410 0.010914
Nuclear Periphery 0.000842 0.000881 0.000817 0.001709 0.001077 0.001178 0.000547 0.000388 0.001065 0.001073 0.000579 0.001554
Nuclear Periphery Foci 0.090278 0.124684 0.053331 0.005521 0.123524 0.085991 0.008280 0.014976 0.121789 0.099636 0.013541 0.006626
Nucleolus 0.150550 0.172295 0.028430 0.037168 0.242248* 0.196725 0.060864 0.015149 0.237462* 0.188110 0.057077 0.034594
Nucleus 0.191695* 0.097447 0.086600 0.195057* 0.079569 0.089596 0.234499* 0.013000 0.085421 0.092365 0.217229* 0.173773*
Peroxisomes 0.039298 0.021340 0.006476 0.002130 0.014155 0.010774 0.001672 0.000958 0.015467 0.014500 0.002233 0.001993
Vacuole 0.003372 0.001630 0.011304 0.006413 0.000550 0.003967 0.001106 0.000431 0.000697 0.003143 0.002297 0.005713
Vacuole Periphery 0.000125 0.000381 0.000198 0.001370 0.000186 0.002105 0.000353 0.000109 0.000183 0.001497 0.000335 0.001223

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.42 -7.90 -11.54 -3.93 5.18 -4.50 -9.90 -4.14 -3.55 -1.79 -9.85 -12.24 -14.85 -9.65 -2.26
Bud Neck 1.12 -4.46 -6.99 -11.03 -2.17 -4.15 -9.04 -5.22 -4.35 -2.77 -4.59 -10.71 -12.84 -11.47 -7.60
Bud Site -0.96 -6.12 -5.83 -5.64 1.96 -1.11 -5.28 -3.17 -3.02 -2.13 -3.24 -7.93 -7.72 -7.04 -4.76
Cell Periphery -2.59 -4.49 -1.36 0.03 2.04 5.41 0.62 3.03 0.42 2.37 0.42 -1.26 -1.47 -1.57 -1.07
Cytoplasm 0.72 0.98 -1.78 -6.41 -6.73 1.72 -2.56 6.98 5.20 7.81 1.14 -2.14 -6.65 -7.47 -4.98
Cytoplasmic Foci 4.18 5.87 5.94 9.94 0.75 9.73 15.66 9.12 3.25 -0.26 8.82 16.61 15.96 11.89 -0.90
Eisosomes 0.57 1.57 6.84 14.41 5.69 8.46 22.53 23.32 17.04 5.86 6.75 20.75 20.86 16.86 2.57
Endoplasmic Reticulum -6.60 -6.49 -5.68 0.21 5.17 -2.34 -3.52 -0.19 0.42 1.35 -7.91 -6.82 -7.54 -3.60 -0.07
Endosome 2.55 1.92 2.23 0.02 0.60 -0.91 12.12 13.38 8.33 1.95 -0.41 10.80 0.62 0.79 -3.13
Golgi -0.83 4.83 4.72 9.16 0.52 7.84 21.33 20.92 16.68 1.08 3.64 21.60 10.78 8.60 -1.71
Lipid Particles 0.75 3.09 3.61 8.66 4.39 3.36 11.97 12.57 8.82 4.26 1.78 12.14 12.20 11.59 0.73
Mitochondria -0.70 1.45 -1.72 -1.10 -4.22 -3.11 1.90 0.03 2.59 -0.65 -3.41 2.60 -3.56 -1.69 -3.93
Mitotic Spindle -1.43 -5.63 -5.94 -9.95 1.05 -6.05 -14.41 -14.55 -11.66 -6.88 -5.02 -15.44 -14.82 -11.51 -0.21
None -2.87 3.67 -0.47 3.08 -6.30 8.41 0.52 10.92 5.19 6.60 4.48 1.24 3.42 0.26 1.60
Nuclear Periphery 0.25 -4.41 -7.04 -7.82 -2.57 -1.56 -1.52 -2.04 -1.50 -0.69 -0.41 -2.25 -7.65 -7.48 -4.91
Nuclear Periphery Foci -1.35 2.36 6.24 21.46* 6.75 9.60 27.51* 18.70 12.25 0.76 7.18 26.27 30.57 30.75* 7.00
Nucleolus -0.06 4.64 4.45 13.93 -1.01 -1.70 12.50 13.16 14.44 5.07 0.16 13.86 20.75 24.26 7.30
Nucleus -0.79 -3.04 -9.43 -15.44 -5.76 -6.95 -19.04 -3.15 -1.36 6.83 -8.49 -19.46 -19.96 -16.67 -2.61
Peroxisomes 1.84 4.19 5.31 12.43 4.24 3.54 14.05 14.73 11.23 1.14 0.33 14.03 16.42 16.76 2.13
Vacuole -0.13 -5.63 -6.03 -6.28 3.89 -3.72 -6.70 -1.86 -1.37 -1.18 -4.54 -7.49 -7.24 -6.17 -2.62
Vacuole Periphery -4.39 -8.37 -4.91 -4.00 -1.95 -2.10 -2.32 -3.25 1.69 0.84 -2.36 -3.00 -5.14 -0.93 -3.00
Standard name
Human Ortholog
Description Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress
Localization
Cell Percentages spindle pole (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cbf2

Cbf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available