Standard name | |
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Human Ortholog | |
Description | Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 2 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 3 | 8 | 1 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 11 | 9 | 5 | 25 | 26 | 32 | 21 | 16 | 13 | 19 | 13 | 17 | 32 | 14 | 10 | 11 | 0 | 0 | 0 | 2 | 2 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 4 | 0 | 0 | 0 | 1 | 1 | 0 |
Endosome | 1 | 6 | 3 | 1 | 0 | 2 | 3 | 4 | 0 | 8 | 2 | 4 | 3 | 3 | 10 | 2 | 2 | 2 | 1 | 5 | 1 | 4 | 6 | 8 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 11 | 27 | 0 | 0 | 14 | 4 | 12 | 10 | 64 | 44 | 22 | 58 | 0 | 1 | 0 | 4 | 3 | 2 | 0 | 1 | 0 | 1 | 1 | 3 |
Nucleus | 40 | 163 | 112 | 154 | 70 | 192 | 205 | 204 | 155 | 205 | 150 | 187 | 85 | 73 | 91 | 54 | 72 | 71 | 35 | 162 | 96 | 79 | 76 | 34 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 |
Nucleolus | 57 | 88 | 29 | 8 | 10 | 12 | 21 | 23 | 32 | 47 | 13 | 35 | 25 | 17 | 30 | 16 | 21 | 17 | 56 | 120 | 72 | 26 | 70 | 43 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 8 | 2 | 3 | 3 | 5 |
Vac/Vac Membrane | 2 | 34 | 18 | 8 | 14 | 27 | 45 | 31 | 33 | 35 | 20 | 18 | 7 | 20 | 24 | 7 | 14 | 25 | 2 | 19 | 12 | 19 | 54 | 45 |
Unique Cell Count | 88 | 273 | 161 | 174 | 98 | 252 | 295 | 287 | 260 | 310 | 198 | 281 | 126 | 125 | 174 | 94 | 115 | 120 | 102 | 324 | 192 | 146 | 226 | 156 |
Labelled Cell Count | 113 | 321 | 176 | 180 | 113 | 265 | 316 | 309 | 312 | 370 | 224 | 335 | 135 | 133 | 189 | 97 | 127 | 132 | 102 | 324 | 192 | 146 | 226 | 156 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.3 | 9.5 | 12.8 | 11.5 | 7.0 | 9.0 | 8.4 | 8.9 | 7.5 | 7.5 | 7.8 | 7.2 | 14.2 | 12.0 | 11.5 | 13.3 | 16.1 | 14.9 | 12.6 | 14.0 | 13.6 |
Std Deviation (1e-4) | 2.6 | 1.9 | 3.0 | 2.4 | 2.0 | 2.7 | 2.4 | 2.1 | 2.0 | 2.0 | 1.8 | 1.8 | 4.3 | 4.0 | 4.2 | 4.0 | 4.8 | 5.2 | 3.9 | 4.0 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.88 | -0.51 | -0.61 | -0.53 | -0.77 | -0.77 | -0.73 | -0.83 | 0.14 | -0.1 | -0.16 | 0.05 | 0.33 | 0.22 | -0.03 | 0.12 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -0.6 | 1.1 | 0.7 | 1.5 | 0.5 | -0.7 | -0.1 | 0 | 1.1 | 1.9 | 3.2 | 2.1 | 0.6 | 0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 4.9 | 0 | 0 | 0 | 6.8 | 5.0 | 4.4 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 4.3 | 0.3 | 1.5 | 0 | 0.3 | -2.1 | -0.8 | 1.3 | -0.7 | -0.4 | -2.0 | -3.2 | -2.0 | -1.2 | -1.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.9 | -1.7 | -4.4 | -3.6 | -3.2 | -1.6 | -0.8 | -3.4 | -1.6 | 0.4 | -1.0 | -0.2 | -0.2 | 0.1 | -0.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.2 | 0.7 | -0.1 | 1.2 | -0.1 | 0.5 | 0 | -0.3 | -1.8 | -1.7 | 1.2 | 0.7 | -1.0 | 0.3 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.7564 | 8.5559 | 7.5916 | 6.7489 | 7.0445 | 7.36 | 12.8937 | 14.1821 | 13.8088 | 13.4395 | 12.5359 | 13.4745 | 9.9465 | 10.8991 | 10.2399 | 8.8909 | 10.1809 | 10.2744 |
Actin | 0.0026 | 0 | 0 | 0 | 0.0001 | 0 | 0.0047 | 0 | 0.002 | 0.0322 | 0 | 0.0026 | 0.0133 | 0 | 0.0001 | 0.0268 | 0.013 | 0 |
Bud | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0004 | 0.0004 | 0.0004 | 0.0008 | 0.0024 | 0.0022 | 0.0013 | 0.0006 | 0.0012 | 0.0005 | 0.0011 | 0.0016 | 0.0011 | 0.0005 | 0.0007 | 0.0055 | 0.0009 | 0.0025 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0005 | 0.0007 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 |
Bud Site | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0012 | 0.0002 | 0.0016 | 0.0007 | 0.0004 | 0.0002 | 0.0012 | 0.0004 | 0.0003 | 0.0019 | 0.0005 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0.005 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0007 | 0.0026 | 0 | 0.0001 | 0.0101 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.01 | 0 | 0.0038 | 0.0047 | 0.0105 | 0.0026 | 0.0049 | 0 | 0.0003 | 0.0046 | 0.0018 | 0.0003 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0026 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0012 | 0 | 0.0001 | 0.0016 | 0 | 0 |
Endosome | 0.0003 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0203 | 0 | 0.0028 | 0.0039 | 0.0108 | 0.0036 | 0.008 | 0.0001 | 0.0028 | 0.0109 | 0.001 | 0.0005 |
Golgi | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0034 | 0 | 0.0016 | 0.0099 | 0.0018 | 0.0012 | 0.0016 | 0 | 0.0006 | 0.003 | 0.0042 | 0.0005 |
Lipid Particles | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0009 | 0.014 | 0 | 0.0058 | 0.0109 | 0.0076 | 0.0039 | 0.0059 | 0 | 0.0009 | 0.0036 | 0.0012 | 0.0035 |
Mitochondria | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.01 | 0.0001 | 0.0021 | 0.0039 | 0.0027 | 0.0003 | 0.0008 | 0.0001 | 0.001 | 0.0015 | 0.0019 | 0.0002 |
None | 0 | 0 | 0 | 0 | 0 | 0 | 0.0058 | 0 | 0.0034 | 0.0002 | 0.0001 | 0.0019 | 0.0027 | 0 | 0 | 0.0205 | 0 | 0 |
Nuclear Periphery | 0.0005 | 0.0034 | 0.0006 | 0.0004 | 0.0041 | 0.0005 | 0.0092 | 0.002 | 0.002 | 0.0131 | 0.001 | 0.0022 | 0.0054 | 0.0003 | 0.0017 | 0.006 | 0.0004 | 0.0002 |
Nucleolus | 0.4181 | 0.436 | 0.4996 | 0.5179 | 0.3348 | 0.4748 | 0.4401 | 0.4627 | 0.5341 | 0.3926 | 0.4479 | 0.5164 | 0.4566 | 0.4104 | 0.5402 | 0.3806 | 0.4753 | 0.5187 |
Nucleus | 0.5689 | 0.558 | 0.4969 | 0.4778 | 0.6519 | 0.5172 | 0.4535 | 0.5317 | 0.4303 | 0.5149 | 0.4974 | 0.457 | 0.4767 | 0.5859 | 0.4437 | 0.5082 | 0.4797 | 0.4701 |
Peroxisomes | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0.0022 | 0 | 0.0022 | 0.0079 | 0.0015 | 0.0011 | 0.0039 | 0 | 0.0001 | 0.0062 | 0.0058 | 0.0001 |
Punctate Nuclear | 0.0036 | 0.001 | 0.0014 | 0.0013 | 0.0027 | 0.0024 | 0.0079 | 0.002 | 0.0021 | 0.002 | 0.011 | 0.003 | 0.0121 | 0.0004 | 0.0061 | 0.0069 | 0.0133 | 0.002 |
Vacuole | 0.0004 | 0.0004 | 0.0003 | 0.0006 | 0.0011 | 0.0007 | 0.0046 | 0.0003 | 0.0017 | 0.0005 | 0.0034 | 0.0008 | 0.0011 | 0.0013 | 0.0008 | 0.0006 | 0.0003 | 0.0006 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0028 | 0.0001 | 0.0005 | 0.0003 | 0.0016 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 47.6237 | 31.4383 | 33.0488 | 32.5829 | 35.1478 | 13.1808 | 30.7939 | 29.0526 | 21.4646 | 35.2131 |
Translational Efficiency | 1.3769 | 1.9327 | 1.4994 | 1.3061 | 1.4675 | 3.9774 | 1.7077 | 1.7971 | 2.0508 | 1.4642 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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