Standard name
Human Ortholog
Description Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; negatively regulates the GTPase cycle of Gtr1p, a Ran/TC4 homolog; subunit of EGO, a vacuolar/endosomal membrane complex that regulates exit from rapamycin-induced growth arrest and endosome to plasma membrane sorting of Gap1p; activates transcription when chromatin bound; activated by the Lst4p-Lst7p GAP complex; homolog of human RagC
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 – – 0.28 0.06 0.12 – – – – – 0 0 0 0.05 0.09 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – – 0 0.06 0 – – – – – 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 – – 0.06 0.06 0 – – – – – 0 0 0 0 0 0 0 0 0.07 0 0 0.05
Golgi 0 0 – – 0.06 0.06 0 – – – – – 0 0 0 0.07 0.08 0.06 0 0 0 0 0 0
Mitochondria 0.19 0 – – 0 0.29 0.31 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.06 – – 0 0 0 – – – – – 0.06 0 0 0.05 0 0 0 0 0.1 0 0 0
Nuclear Periphery 0.2 0.14 – – 0.06 0.11 0.19 – – – – – 0.08 0.19 0.17 0.23 0.15 0.26 0.08 0 0.2 0.09 0.12 0.09
Nucleolus 0.1 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0.06 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.74 0.86 – – 0.67 0.66 0.56 – – – – – 0.89 0.92 0.9 0.86 0.84 0.84 0.83 0.86 0.57 0.81 0.76 0.77
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 1 0 0 1 2
Bud 1 0 – – 0 0 0 – – – – – 0 0 1 0 0 0 0 3 0 3 3 5
Bud Neck 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 1 0 – – – – – 0 1 0 4 1 2 0 0 0 0 0 0
Cytoplasm 3 19 – – 5 2 2 – – – – – 15 19 21 18 14 22 0 0 0 2 1 0
Endoplasmic Reticulum 0 1 – – 0 2 0 – – – – – 1 0 3 16 6 19 0 2 0 1 1 1
Endosome 0 1 – – 1 2 0 – – – – – 15 5 12 2 0 7 7 3 0 18 17 31
Golgi 0 2 – – 1 2 0 – – – – – 2 5 8 24 13 23 1 1 0 1 2 3
Mitochondria 32 5 – – 0 10 5 – – – – – 6 1 1 10 4 17 3 3 0 7 2 5
Nucleus 14 17 – – 0 1 0 – – – – – 28 10 17 18 5 17 2 10 1 8 11 16
Nuclear Periphery 34 43 – – 1 4 3 – – – – – 37 95 90 80 23 100 13 13 2 44 53 53
Nucleolus 17 2 – – 0 1 0 – – – – – 9 1 3 10 3 12 1 1 0 2 4 6
Peroxisomes 0 0 – – 0 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 1 1 0 – – – – – 0 0 1 0 0 0 1 0 0 0 1 1
Vac/Vac Membrane 125 255 – – 12 23 9 – – – – – 394 462 464 296 132 319 143 264 8 399 336 447
Unique Cell Count 168 297 18 35 16 442 502 515 345 157 380 174 309 15 490 442 582
Labelled Cell Count 226 345 21 49 19 507 599 621 478 201 538 174 309 15 490 442 582


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 11.0 – – 9.6 7.9 7.5 – – – – – 10.1 10.9 11.0 14.3 13.9 14.0 10.5 10.6 11.1
Std Deviation (1e-4) 1.4 2.7 – – 1.6 1.7 2.3 – – – – – 2.2 2.1 1.9 4.0 3.8 3.6 2.2 2.2 2.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2254 4.7414 4.2629 4.016 4.0073 4.1095 4.467 4.7918 4.4167 4.3206 4.2179 4.5942 5.2192 5.8983 5.6074 5.3327 4.716 5.893
Actin 0.0102 0 0.0009 0 0.0047 0 0.0176 0.0011 0.0037 0.0037 0 0.0044 0 0 0 0 0 0
Bud 0.0002 0.0008 0.0104 0.0002 0.0054 0.0029 0.0004 0.0027 0.0025 0.0004 0 0.0005 0.0001 0.0005 0.0005 0.0001 0.0001 0.0003
Bud Neck 0.0004 0.0002 0.0004 0.0001 0.0005 0.0004 0.0029 0.0004 0.0009 0.0006 0 0.0011 0.0001 0.0002 0.0003 0 0.0001 0.0001
Bud Periphery 0.0004 0.0009 0.003 0.0002 0.0017 0.0018 0.0006 0.0035 0.0025 0.0007 0.0001 0.0011 0.0002 0.0008 0.0011 0.0005 0.0002 0.0006
Bud Site 0.0004 0.0014 0.0054 0.0001 0.0014 0.0008 0.0033 0.0065 0.0044 0.0024 0 0.0021 0.0001 0.001 0.0049 0 0.0001 0.0002
Cell Periphery 0.0005 0.0007 0.001 0.0003 0.0008 0.0005 0.0007 0.001 0.0011 0.0005 0.0001 0.0004 0.0004 0.0007 0.0006 0.0002 0.0002 0.0004
Cytoplasm 0.0077 0.0075 0.0129 0.0024 0.0037 0.0038 0.0132 0.0087 0.0052 0.0038 0.0003 0.007 0.0032 0.0026 0.0016 0.0005 0.0007 0.0017
Cytoplasmic Foci 0.0065 0.0029 0.0035 0.0003 0.009 0.0214 0.0211 0.0054 0.0045 0.024 0.0002 0.0066 0.0026 0.0008 0.0004 0.0002 0.0012 0.0013
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0039 0.001 0.0021 0.0002 0.0026 0.0009 0.0045 0.001 0.0018 0.0059 0.0003 0.0022 0.0009 0.0006 0.0029 0.0019 0.0006 0.0007
Endosome 0.073 0.0359 0.069 0.0327 0.034 0.0602 0.1026 0.0441 0.0741 0.071 0.044 0.0815 0.0413 0.027 0.0392 0.0257 0.0239 0.0283
Golgi 0.003 0.0011 0.0023 0.0001 0.003 0.0012 0.0075 0.0014 0.0101 0.009 0.0004 0.0051 0.0007 0.0004 0.0007 0.0003 0.0003 0.0006
Lipid Particles 0.0046 0.0007 0.002 0.0001 0.0069 0.0025 0.0249 0.002 0.0037 0.0314 0.0001 0.0055 0.0013 0.0003 0.0004 0.0001 0.0008 0.0006
Mitochondria 0.006 0.0044 0.0303 0.0001 0.0057 0.0057 0.0079 0.003 0.0231 0.0044 0.0002 0.0143 0.0016 0.0014 0.0114 0.0013 0.0013 0.0034
None 0.0031 0.0002 0.0058 0 0.0046 0.0009 0.004 0.0017 0.0004 0.002 0 0.0009 0.0041 0.0001 0 0 0.0001 0.0001
Nuclear Periphery 0.0203 0.0118 0.0164 0.001 0.0823 0.0368 0.0271 0.0096 0.0137 0.0113 0.0029 0.0226 0.0141 0.0076 0.0102 0.0103 0.0224 0.0212
Nucleolus 0.0021 0.0042 0.0017 0.0002 0.0082 0.0086 0.0027 0.0142 0.0115 0.002 0.0001 0.0017 0.001 0.0013 0.0031 0.0001 0.0005 0.0008
Nucleus 0.0243 0.0095 0.0052 0.0012 0.0073 0.0082 0.0256 0.0067 0.0053 0.003 0.0004 0.0028 0.0084 0.0048 0.0023 0.0006 0.0011 0.0062
Peroxisomes 0.0018 0.0003 0.0005 0 0.0016 0.0003 0.0123 0.001 0.001 0.0037 0 0.0011 0.0001 0.0001 0.0001 0 0.0001 0.0003
Punctate Nuclear 0.0026 0.0001 0.0027 0 0.0046 0.0003 0.0096 0.0018 0.0003 0.0079 0 0.0034 0.0011 0 0 0 0 0
Vacuole 0.3921 0.4663 0.3544 0.5448 0.2702 0.2942 0.3478 0.4046 0.3712 0.3573 0.3449 0.2578 0.3681 0.4174 0.4003 0.3408 0.226 0.2844
Vacuole Periphery 0.4368 0.4502 0.47 0.416 0.5415 0.5484 0.3635 0.4794 0.4586 0.4548 0.6057 0.578 0.5506 0.5324 0.5198 0.6175 0.7205 0.6489

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5281 4.8069 12.1276 18.3093 13.9436 11.8233 17.2539 19.354 20.3765 20.3546
Translational Efficiency 3.0865 5.3435 1.8837 1.3866 1.4859 2.0606 1.6965 1.1791 1.155 1.2713

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1780 728 1704 576 1737 2234 2307 927 3517 2962 4011 1503

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 874.00 1049.80 1068.50 1204.85 901.98 1009.99 1119.25 1213.42 887.82 1019.77 1097.69 1210.14
Standard Deviation 155.25 180.04 169.09 229.31 145.26 157.00 168.06 209.78 151.05 163.86 170.36 217.51
Intensity Change Log 2 — 0.264409 0.289882 0.463148 — 0.163174 0.311365 0.427912 — 0.213882 0.300833 0.445360

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000013 0.000028 0.000075 0.000279 0.000013 0.000049 0.000051 0.000057 0.000013 0.000044 0.000062 0.000142
Bud Neck 0.000372 0.000600 0.003125 0.003636 0.000287 0.001330 0.003159 0.002681 0.000330 0.001151 0.003145 0.003047
Bud Site 0.004399 0.002572 0.003619 0.008841 0.001483 0.002357 0.002544 0.005506 0.002959 0.002410 0.003001 0.006784
Cell Periphery 0.000655 0.000293 0.000443 0.000332 0.000272 0.000235 0.000421 0.000277 0.000466 0.000249 0.000431 0.000298
Cytoplasm 0.006895 0.002477 0.000964 0.001208 0.001753 0.000854 0.000460 0.002193 0.004356 0.001253 0.000674 0.001815
Cytoplasmic Foci 0.002397 0.003913 0.002267 0.007736 0.001265 0.004762 0.002335 0.004153 0.001838 0.004554 0.002306 0.005526
Eisosomes 0.000003 0.000003 0.000041 0.000024 0.000002 0.000010 0.000032 0.000010 0.000003 0.000008 0.000036 0.000015
Endoplasmic Reticulum 0.001397 0.000646 0.001226 0.000674 0.001037 0.000742 0.001112 0.000642 0.001219 0.000719 0.001160 0.000654
Endosome 0.011741 0.011298 0.007575 0.027507 0.006083 0.016090 0.009078 0.014135 0.008947 0.014912 0.008440 0.019260
Golgi 0.002062 0.003731 0.002412 0.029999 0.001067 0.005679 0.002627 0.011726 0.001570 0.005200 0.002536 0.018729
Lipid Particles 0.000141 0.000358 0.000547 0.001214 0.000254 0.000885 0.000774 0.000290 0.000197 0.000755 0.000678 0.000644
Mitochondria 0.003930 0.005689 0.005710 0.015269 0.004154 0.013805 0.007638 0.004813 0.004041 0.011811 0.006819 0.008820
Mitotic Spindle 0.000088 0.001213 0.000950 0.005578 0.000067 0.001843 0.000765 0.001686 0.000077 0.001688 0.000844 0.003178
None 0.000439 0.000179 0.000322 0.000288 0.000411 0.000355 0.000324 0.000209 0.000425 0.000311 0.000323 0.000239
Nuclear Periphery 0.000216 0.000912 0.001691 0.004516 0.000497 0.002194 0.002140 0.001328 0.000355 0.001879 0.001950 0.002550
Nuclear Periphery Foci 0.000159 0.002465 0.000874 0.005863 0.000158 0.003295 0.001486 0.000955 0.000159 0.003091 0.001226 0.002836
Nucleolus 0.000714 0.000651 0.012620 0.002772 0.000235 0.002837 0.008760 0.002913 0.000477 0.002300 0.010400 0.002859
Nucleus 0.000787 0.000503 0.009013 0.001635 0.000369 0.002060 0.009014 0.003625 0.000580 0.001678 0.009013 0.002862
Peroxisomes 0.000205 0.000346 0.000592 0.001996 0.000122 0.000991 0.000599 0.000268 0.000164 0.000833 0.000596 0.000930
Vacuole 0.210585* 0.198649 0.405373* 0.416347* 0.211556* 0.212730* 0.392974* 0.468284* 0.211064* 0.209269* 0.398242* 0.448380*
Vacuole Periphery 0.752803* 0.763471* 0.540560* 0.464285* 0.768915* 0.726895* 0.553703* 0.474250* 0.760760* 0.735885* 0.548120* 0.470431*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.52 -3.94 -3.31 -3.09 -2.47 -4.34 -4.14 -3.10 -0.71 -0.60 -5.02 -5.68 -4.08 -3.05 -2.48
Bud Neck -1.58 -9.19 -6.38 -5.60 -0.80 -5.22 -7.95 -5.85 -3.41 0.28 -5.48 -11.61 -8.42 -5.62 -0.20
Bud Site 2.28 0.98 -2.41 -3.34 -2.82 -2.15 -2.46 -4.41 -3.52 -3.25 1.19 -0.26 -4.31 -4.89 -4.23
Cell Periphery 3.47 2.19 3.29 -0.54 1.85 0.91 -2.85 -0.06 -0.89 2.63 4.14 0.64 3.00 -1.30 3.25
Cytoplasm 2.63 4.79 4.65 1.18 -0.54 1.58 2.23 -2.20 -3.76 -4.25 4.25 5.39 1.91 -2.63 -4.04
Cytoplasmic Foci -1.64 0.16 -3.25 -1.87 -3.16 -5.29 -1.82 -2.93 0.56 -1.66 -4.99 -1.15 -4.22 -0.87 -3.40
Eisosomes -0.51 -12.68 -5.28 -5.15 3.52 -8.34 -14.77 -7.61 -0.02 9.93 -7.79 -19.27 -7.71 -3.92 8.81
Endoplasmic Reticulum 4.69 0.87 4.10 -0.28 3.04 2.61 -0.58 3.12 0.73 3.70 5.17 0.50 5.33 0.51 4.78
Endosome 0.20 3.33 -5.28 -5.02 -6.97 -7.46 -2.91 -5.26 1.04 -3.40 -5.28 0.59 -6.85 -2.68 -7.52
Golgi -2.38 -1.01 -7.74 -7.16 -7.63 -7.97 -5.42 -6.12 -3.26 -5.18 -7.70 -4.24 -9.71 -7.40 -9.13
Lipid Particles -1.47 -9.40 -5.53 -3.49 -3.42 -3.14 -4.21 -0.54 3.08 4.68 -3.77 -6.95 -5.00 0.63 0.22
Mitochondria -1.11 -1.75 -4.89 -3.54 -3.94 -5.83 -3.00 -0.50 5.26 2.30 -6.26 -3.60 -4.11 1.94 -1.63
Mitotic Spindle -2.28 -2.07 -2.62 -2.05 -2.03 -3.92 -2.51 -2.83 -0.25 -1.66 -4.44 -3.14 -3.63 -1.78 -2.54
None 3.09 1.33 1.80 -2.88 0.77 0.23 0.35 0.79 1.96 1.60 0.87 0.78 1.38 1.43 1.77
Nuclear Periphery -2.68 -7.77 -3.10 -2.56 -2.02 -7.06 -5.06 -2.65 2.18 1.78 -8.00 -7.86 -3.89 -1.16 -1.03
Nuclear Periphery Foci -2.48 -6.10 -3.21 -1.68 -2.80 -6.76 -4.82 -2.59 4.17 1.24 -7.02 -6.42 -3.79 0.32 -2.22
Nucleolus -0.18 -9.65 -3.79 -3.40 7.73 -7.66 -12.91 -4.69 -0.36 6.32 -5.95 -15.16 -5.65 -1.28 9.84
Nucleus 0.81 -9.33 -2.12 -3.02 7.99 -4.21 -9.07 -3.84 -2.13 3.15 -3.39 -12.66 -4.14 -2.35 5.97
Peroxisomes -1.18 -2.98 -2.78 -2.47 -2.16 -2.89 -4.04 -2.66 2.34 2.54 -2.92 -4.81 -3.08 -0.29 -1.30
Vacuole 1.49 -26.14 -19.32 -18.64 -1.04 -0.20 -26.85 -28.01 -29.23 -8.39 0.38 -37.59 -34.14 -34.37 -7.24
Vacuole Periphery -0.97 24.89 23.30* 21.45 5.97 5.75 28.71 29.70* 25.53* 8.43 4.32 37.75 37.59 32.99* 10.25
Standard name
Human Ortholog
Description Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; negatively regulates the GTPase cycle of Gtr1p, a Ran/TC4 homolog; subunit of EGO, a vacuolar/endosomal membrane complex that regulates exit from rapamycin-induced growth arrest and endosome to plasma membrane sorting of Gap1p; activates transcription when chromatin bound; activated by the Lst4p-Lst7p GAP complex; homolog of human RagC
Localization
Cell Percentages vacuolar membrane (95%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Gtr2

Gtr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available