Standard name | |
---|---|
Human Ortholog | |
Description | Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 149 | 104 | 82 | 109 | 108 | 110 | 129 | 127 | 105 | 138 | 121 | 146 | 89 | 112 | 105 | 163 | 140 | 127 | 78 | 68 | 42 | 2 | 12 | 18 |
Bud | 62 | 51 | 36 | 47 | 71 | 51 | 61 | 68 | 63 | 53 | 73 | 69 | 23 | 44 | 39 | 74 | 57 | 68 | 96 | 87 | 71 | 24 | 68 | 74 |
Bud Neck | 3 | 1 | 3 | 3 | 2 | 2 | 3 | 6 | 4 | 5 | 4 | 4 | 0 | 2 | 0 | 4 | 16 | 5 | 57 | 49 | 43 | 14 | 49 | 34 |
Bud Site | 69 | 20 | 16 | 28 | 25 | 37 | 28 | 23 | 32 | 29 | 19 | 32 | 26 | 20 | 2 | 23 | 27 | 27 | – | – | – | – | – | – |
Cell Periphery | 33 | 27 | 22 | 43 | 12 | 24 | 37 | 27 | 46 | 31 | 29 | 53 | 33 | 57 | 99 | 44 | 39 | 25 | 29 | 9 | 16 | 0 | 0 | 0 |
Cytoplasm | 16 | 27 | 22 | 30 | 42 | 44 | 53 | 67 | 40 | 31 | 33 | 45 | 35 | 32 | 42 | 59 | 53 | 47 | 0 | 4 | 3 | 4 | 8 | 19 |
Endoplasmic Reticulum | 9 | 8 | 9 | 14 | 9 | 15 | 34 | 28 | 19 | 29 | 27 | 16 | 25 | 18 | 28 | 8 | 13 | 8 | 3 | 2 | 1 | 0 | 1 | 0 |
Endosome | 1 | 3 | 0 | 3 | 3 | 1 | 3 | 0 | 3 | 3 | 1 | 5 | 1 | 3 | 3 | 4 | 5 | 5 | 1 | 0 | 1 | 0 | 2 | 0 |
Golgi | 2 | 2 | 1 | 1 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 1 | 1 | 4 | 48 | 25 | 30 | 3 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 44 | 7 | 1 | 3 | 0 | 24 | 21 | 31 | 84 | 64 | 170 | 105 | 2 | 1 | 3 | 41 | 26 | 32 | 4 | 3 | 5 | 0 | 0 | 0 |
Nucleus | 2 | 7 | 7 | 4 | 8 | 12 | 20 | 12 | 7 | 7 | 9 | 15 | 8 | 7 | 3 | 23 | 8 | 10 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 6 | 3 | 0 | 4 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 3 | 0 | 2 | 7 | 6 | 8 | 12 | 9 | 9 | 11 | 24 | 0 | 4 | 0 | 11 | 6 | 14 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 15 | 4 | 3 | 7 | 2 | 8 | 13 | 11 | 19 | 22 | 17 | 30 | 8 | 13 | 29 | 13 | 25 | 21 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 7 | 6 | 13 | 5 | 17 | 11 | 10 | 20 | 10 | 13 | 4 | 15 | 15 | 32 | 26 | 35 | 37 | 38 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 9 | 6 | 3 | 3 | 10 | 9 | 10 | 17 | 16 | 8 | 15 | 7 | 7 | 5 | 20 | 9 | 11 | 1 | 4 | 2 | 0 | 0 | 1 |
Unique Cell Count | 234 | 178 | 142 | 191 | 206 | 228 | 278 | 294 | 291 | 284 | 299 | 364 | 186 | 229 | 260 | 366 | 308 | 299 | 282 | 238 | 191 | 51 | 149 | 156 |
Labelled Cell Count | 419 | 279 | 221 | 302 | 309 | 356 | 431 | 445 | 458 | 453 | 532 | 578 | 273 | 357 | 389 | 571 | 486 | 468 | 282 | 238 | 191 | 51 | 149 | 156 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 10.6 | 9.5 | 9.1 | 9.2 | 8.3 | 8.1 | 8.1 | 7.3 | 7.3 | 6.6 | 7.1 | 9.9 | 10.0 | 10.2 | 12.6 | 12.2 | 11.6 | 10.6 | 9.7 | 10.2 |
Std Deviation (1e-4) | 1.6 | 2.6 | 2.0 | 1.5 | 2.2 | 2.2 | 1.9 | 1.8 | 1.7 | 1.6 | 1.4 | 1.5 | 2.2 | 2.4 | 2.3 | 4.2 | 4.2 | 3.8 | 2.1 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.06 | -0.2 | -0.24 | -0.23 | -0.38 | -0.38 | -0.53 | -0.43 | 0.05 | 0.07 | 0.1 | 0.4 | 0.36 | 0.29 | 0.15 | 0.03 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | -1.0 | -1.8 | -2.2 | -2.8 | -4.3 | -1.8 | -3.4 | -3.6 | -1.8 | -1.7 | -3.3 | -2.7 | -2.4 | -3.0 |
Bud | -0.2 | 1.8 | -0.7 | -0.8 | -0.5 | -0.9 | -1.6 | -0.2 | -1.6 | -3.0 | -1.4 | -2.5 | -1.3 | -1.7 | -0.6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 |
Bud Site | 0.9 | 0.2 | 1.3 | -0.4 | -1.2 | -0.1 | -0.3 | -1.8 | -0.9 | 0.7 | -0.8 | -4.9 | -1.9 | -0.8 | -0.7 |
Cell Periphery | 1.6 | -3.0 | -1.4 | -0.6 | -2.0 | 0.1 | -1.3 | -1.8 | -0.3 | 0.5 | 2.1 | 4.7 | -1.0 | -0.8 | -2.3 |
Cytoplasm | 0.1 | 1.2 | 0.9 | 0.9 | 1.8 | -0.5 | -1.3 | -1.3 | -0.9 | 0.8 | -0.4 | 0.2 | 0.2 | 0.5 | 0.1 |
Endoplasmic Reticulum | 0.4 | -0.8 | 0.1 | 1.9 | 1.1 | 0.1 | 1.3 | 1.0 | -0.9 | 2.1 | 0.5 | 1.5 | 0 | -1.0 | -1.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 3.1 | 3.6 |
Mitochondria | 0 | 0 | 3.7 | 3.0 | 3.7 | 6.9 | 5.9 | 11.3 | 7.0 | 0 | 0 | 0 | 3.9 | 3.2 | 3.7 |
Nucleus | 0 | -0.5 | 0.1 | 0.9 | -0.4 | 0 | 0 | -1.0 | -0.4 | -0.3 | -0.9 | 0 | 0.6 | 0 | -0.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1.3 | 0 | 2.0 | 2.3 | 1.7 | 2.5 | 0 | 1.6 | 3.2 | 0 | 2.5 | 2.1 |
SpindlePole | -2.6 | -0.3 | -1.6 | -2.4 | -0.9 | -2.5 | -1.9 | -4.0 | -2.2 | -0.4 | 1.4 | 0.3 | 0.1 | 0.9 | 1.1 |
Vacuole | 0 | 0 | 0.1 | -0.5 | -0.4 | 0.7 | 0.6 | 0 | -0.1 | -0.2 | 0 | 0 | 0.6 | 0 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.5826 | 5.0796 | 5.2029 | 5.5727 | 4.9884 | 5.6679 | 4.5366 | 4.5413 | 4.6073 | 3.3403 | 4.3862 | 5.4315 | 4.7128 | 5.2291 | 4.6257 | 5.2348 | 3.1636 | 5.553 |
Actin | 0.2117 | 0.1099 | 0.1177 | 0.1065 | 0.4105 | 0.1765 | 0.214 | 0.0748 | 0.164 | 0.2209 | 0.0932 | 0.1664 | 0.2602 | 0.1223 | 0.078 | 0.2548 | 0.1016 | 0.2256 |
Bud | 0.04 | 0.0741 | 0.25 | 0.1069 | 0.055 | 0.0155 | 0.0237 | 0.0464 | 0.2137 | 0.0572 | 0.0441 | 0.0069 | 0.0358 | 0.0233 | 0.3564 | 0.0879 | 0.0424 | 0.0077 |
Bud Neck | 0.0655 | 0.0075 | 0.0111 | 0.0405 | 0.0354 | 0.6015 | 0.0781 | 0.0203 | 0.0172 | 0.0056 | 0.1496 | 0.6823 | 0.0737 | 0.0353 | 0.0105 | 0.0052 | 0.0013 | 0.6416 |
Bud Periphery | 0.1179 | 0.0968 | 0.4612 | 0.6177 | 0.3534 | 0.0868 | 0.0863 | 0.0698 | 0.3928 | 0.4114 | 0.2825 | 0.0345 | 0.1031 | 0.0344 | 0.4254 | 0.4952 | 0.2837 | 0.0398 |
Bud Site | 0.3353 | 0.5922 | 0.1207 | 0.0325 | 0.0357 | 0.0313 | 0.3173 | 0.7192 | 0.1329 | 0.0118 | 0.0664 | 0.0204 | 0.2393 | 0.6547 | 0.0791 | 0.0227 | 0.0042 | 0.0134 |
Cell Periphery | 0.121 | 0.0152 | 0.017 | 0.0291 | 0.0326 | 0.057 | 0.0894 | 0.0205 | 0.0167 | 0.0263 | 0.0456 | 0.0377 | 0.0589 | 0.0173 | 0.0088 | 0.0172 | 0.014 | 0.0219 |
Cytoplasm | 0.0371 | 0.0639 | 0.0114 | 0.0314 | 0.0521 | 0.0091 | 0.0277 | 0.0301 | 0.0146 | 0.0077 | 0.0463 | 0.0129 | 0.0463 | 0.0723 | 0.0084 | 0.0359 | 0.0126 | 0.0073 |
Cytoplasmic Foci | 0.0041 | 0.0133 | 0.0005 | 0.0003 | 0.0008 | 0.0022 | 0.027 | 0.0015 | 0.0051 | 0.0303 | 0.0224 | 0.0034 | 0.0245 | 0.0028 | 0.0035 | 0.0111 | 0.0108 | 0.0027 |
Eisosomes | 0.0316 | 0.0007 | 0.0005 | 0.0273 | 0.0028 | 0.0059 | 0.0096 | 0.0004 | 0.0007 | 0.0031 | 0.001 | 0.0022 | 0.0112 | 0.0037 | 0.0147 | 0.002 | 0.0007 | 0.002 |
Endoplasmic Reticulum | 0.0094 | 0.0009 | 0.0007 | 0.0019 | 0.0016 | 0.0011 | 0.0117 | 0.0006 | 0.0011 | 0.004 | 0.0073 | 0.0016 | 0.0082 | 0.0025 | 0.0005 | 0.002 | 0.0025 | 0.0004 |
Endosome | 0.004 | 0.0006 | 0.0002 | 0.0003 | 0.0009 | 0.0009 | 0.0231 | 0.0018 | 0.0099 | 0.1043 | 0.0487 | 0.0049 | 0.0347 | 0.0059 | 0.0016 | 0.016 | 0.1342 | 0.0103 |
Golgi | 0.0035 | 0.0014 | 0.0004 | 0.0008 | 0.0022 | 0.0021 | 0.009 | 0.001 | 0.0099 | 0.0534 | 0.0141 | 0.0023 | 0.0279 | 0.0029 | 0.0045 | 0.0392 | 0.0708 | 0.0071 |
Lipid Particles | 0.002 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0155 | 0.0015 | 0.0039 | 0.0142 | 0.0576 | 0.0012 | 0.0139 | 0.0015 | 0.0021 | 0.0018 | 0.0134 | 0.0057 |
Mitochondria | 0.0032 | 0.0023 | 0.001 | 0.001 | 0.01 | 0.0014 | 0.0102 | 0.0006 | 0.0086 | 0.0267 | 0.0415 | 0.0008 | 0.0258 | 0.0035 | 0.0024 | 0.0045 | 0.1641 | 0.0082 |
None | 0.0018 | 0.0019 | 0.0025 | 0.0005 | 0.0009 | 0.0004 | 0.0042 | 0.0037 | 0.0006 | 0.003 | 0.0024 | 0.0031 | 0.0034 | 0.0006 | 0.0007 | 0.0005 | 0.0066 | 0.0001 |
Nuclear Periphery | 0.0018 | 0.0007 | 0.0004 | 0.0006 | 0.0006 | 0.0004 | 0.0103 | 0.0005 | 0.0006 | 0.0013 | 0.0137 | 0.0015 | 0.0123 | 0.0115 | 0.0002 | 0.0006 | 0.0125 | 0.0002 |
Nucleolus | 0.0008 | 0.0007 | 0.0002 | 0.0001 | 0.0006 | 0.0011 | 0.0016 | 0.0006 | 0.0002 | 0.0007 | 0.0027 | 0.0007 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0046 | 0.0002 |
Nucleus | 0.0013 | 0.0014 | 0.0007 | 0.0004 | 0.001 | 0.0011 | 0.0096 | 0.0014 | 0.0006 | 0.0008 | 0.0063 | 0.0031 | 0.0057 | 0.0014 | 0.0003 | 0.0003 | 0.088 | 0.0004 |
Peroxisomes | 0.0009 | 0.0048 | 0.0002 | 0.0001 | 0.001 | 0.0012 | 0.0109 | 0.0006 | 0.0039 | 0.0073 | 0.0361 | 0.0008 | 0.0062 | 0.0006 | 0.0023 | 0.0016 | 0.0029 | 0.0012 |
Punctate Nuclear | 0.0006 | 0.0086 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0104 | 0.0004 | 0.0003 | 0.0012 | 0.0033 | 0.0055 | 0.002 | 0.0003 | 0.0002 | 0.0002 | 0.0072 | 0.0002 |
Vacuole | 0.0058 | 0.0024 | 0.0026 | 0.0016 | 0.0022 | 0.003 | 0.0079 | 0.0042 | 0.0021 | 0.0065 | 0.0105 | 0.0076 | 0.0046 | 0.0011 | 0.0004 | 0.0009 | 0.013 | 0.0032 |
Vacuole Periphery | 0.0006 | 0.0003 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0023 | 0.0003 | 0.0005 | 0.0024 | 0.0047 | 0.0003 | 0.0014 | 0.0018 | 0.0001 | 0.0003 | 0.009 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.7152 | 20.2245 | 17.1513 | 26.8879 | 18.8569 | 18.0892 | 22.786 | 27.209 | 22.951 | 25.2589 |
Translational Efficiency | 1.1519 | 1.1454 | 1.2381 | 0.6249 | 1.0127 | 1.3208 | 0.9478 | 0.8156 | 1.0543 | 0.8936 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|