Standard name | |
---|---|
Human Ortholog | |
Description | Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 12 | 1 | 4 | 2 | 6 | 10 | 10 | 40 | 5 | 11 | 23 |
Bud | 5 | 2 | 3 | 12 | 6 | 13 | 23 | 18 | 27 | 31 | 20 | 20 | 1 | 1 | 0 | 1 | 2 | 2 | 3 | 0 | 4 | 2 | 5 | 10 |
Bud Neck | 0 | 1 | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 5 | 7 | 2 | 0 | 2 | 0 | 5 | 3 | 6 | 6 | 5 | 13 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 5 | 16 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 4 | 4 | 3 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 4 | 3 | 0 | 1 | 0 | 3 | 0 | 5 | 9 | 12 | 17 |
Cytoplasm | 158 | 101 | 226 | 236 | 376 | 299 | 333 | 272 | 162 | 166 | 84 | 80 | 247 | 321 | 288 | 8 | 21 | 24 | 62 | 81 | 65 | 198 | 273 | 294 |
Endoplasmic Reticulum | 1 | 34 | 4 | 1 | 1 | 7 | 3 | 2 | 3 | 1 | 1 | 6 | 32 | 27 | 40 | 1 | 8 | 6 | 79 | 35 | 101 | 38 | 42 | 50 |
Endosome | 3 | 1 | 20 | 10 | 11 | 16 | 23 | 19 | 14 | 6 | 2 | 4 | 5 | 19 | 28 | 25 | 34 | 55 | 5 | 6 | 14 | 3 | 10 | 18 |
Golgi | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 3 | 1 | 1 | 6 | 0 | 1 | 0 |
Mitochondria | 51 | 18 | 7 | 36 | 52 | 159 | 369 | 397 | 402 | 500 | 318 | 413 | 1 | 1 | 0 | 4 | 9 | 18 | 60 | 24 | 64 | 38 | 40 | 62 |
Nucleus | 1 | 0 | 0 | 0 | 2 | 4 | 4 | 4 | 2 | 4 | 5 | 8 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 3 | 3 | 2 | 1 | 1 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 3 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 9 | 11 | 4 | 2 | 1 | 0 | 3 | 3 | 0 | 3 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 10 | 7 | 4 | 5 | 8 | 21 | 6 | 16 | 5 | 11 | 4 | 13 | 29 | 10 | 12 | 17 | 2 | 2 | 2 | 3 | 5 | 7 |
Vac/Vac Membrane | 4 | 0 | 6 | 4 | 4 | 12 | 30 | 40 | 30 | 51 | 28 | 37 | 1 | 11 | 5 | 6 | 7 | 11 | 10 | 6 | 9 | 12 | 14 | 22 |
Unique Cell Count | 203 | 150 | 271 | 279 | 424 | 428 | 649 | 647 | 540 | 641 | 374 | 471 | 290 | 401 | 380 | 49 | 82 | 110 | 251 | 179 | 335 | 328 | 431 | 533 |
Labelled Cell Count | 228 | 165 | 295 | 323 | 463 | 525 | 805 | 781 | 657 | 798 | 470 | 598 | 303 | 421 | 398 | 61 | 100 | 144 | 251 | 179 | 335 | 328 | 431 | 533 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.6 | 4.8 | 4.4 | 4.3 | 3.9 | 3.6 | 3.9 | 3.6 | 3.6 | 3.4 | 3.5 | 5.7 | 5.7 | 5.6 | 6.9 | 6.5 | 6.4 | 5.8 | 5.9 | 6.2 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.4 | 2.0 | 1.6 | 1.7 | 1.4 | 1.6 | 1.2 | 0.9 | 1.3 | 1.3 | 0.9 | 1.1 | 1.0 | 1.4 | 1.3 | 1.2 | 1.1 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.15 | -0.31 | -0.4 | -0.3 | -0.42 | -0.43 | -0.51 | -0.45 | 0.25 | 0.26 | 0.22 | 0.52 | 0.44 | 0.42 | 0.29 | 0.3 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3478 | 2.7268 | 2.4717 | 2.5252 | 1.9419 | 2.7515 | -0.1352 | 0.0177 | -0.0088 | -0.6801 | -0.83 | -0.3719 | -1.2679 | -0.8568 | -1.1226 | -1.1347 | -0.7471 | -1.15 |
Actin | 0.0171 | 0.0027 | 0.0143 | 0.0012 | 0.0042 | 0.003 | 0.05 | 0.0026 | 0.0065 | 0.0012 | 0.0136 | 0.0122 | 0.0352 | 0.0005 | 0.0063 | 0.0057 | 0.0529 | 0.0059 |
Bud | 0.0003 | 0.0003 | 0.0019 | 0.0004 | 0.0004 | 0.0001 | 0.0068 | 0.0028 | 0.001 | 0.0001 | 0.0006 | 0.0008 | 0.0011 | 0.0003 | 0.0002 | 0.0004 | 0.0005 | 0.0002 |
Bud Neck | 0.002 | 0.0003 | 0.0092 | 0.0007 | 0.0003 | 0.0011 | 0.0066 | 0.0006 | 0.0048 | 0.0004 | 0.004 | 0.0026 | 0.0075 | 0.0004 | 0.0031 | 0.0056 | 0.0032 | 0.0084 |
Bud Periphery | 0.0012 | 0.0006 | 0.0048 | 0.001 | 0.0009 | 0.0003 | 0.0056 | 0.0013 | 0.0009 | 0.0001 | 0.0016 | 0.0017 | 0.0012 | 0.0004 | 0.0002 | 0.0006 | 0.0011 | 0.0003 |
Bud Site | 0.0044 | 0.0035 | 0.0086 | 0.0016 | 0.0006 | 0.0005 | 0.0124 | 0.0162 | 0.0079 | 0.0003 | 0.0008 | 0.0018 | 0.0089 | 0.0038 | 0.0015 | 0.0025 | 0.0014 | 0.0002 |
Cell Periphery | 0.0034 | 0.0018 | 0.0048 | 0.0009 | 0.0004 | 0.0007 | 0.0016 | 0.001 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0011 | 0.0005 | 0.0007 | 0.0002 | 0.0004 | 0.0009 |
Cytoplasm | 0.1263 | 0.2195 | 0.2232 | 0.1758 | 0.1673 | 0.2037 | 0.1661 | 0.4049 | 0.3729 | 0.4613 | 0.379 | 0.4669 | 0.1644 | 0.2275 | 0.2915 | 0.3423 | 0.1949 | 0.283 |
Cytoplasmic Foci | 0.0545 | 0.0415 | 0.0447 | 0.0738 | 0.0324 | 0.0412 | 0.0693 | 0.069 | 0.0623 | 0.0346 | 0.0907 | 0.0541 | 0.1181 | 0.0793 | 0.0702 | 0.0973 | 0.1205 | 0.0662 |
Eisosomes | 0.0041 | 0.0007 | 0.001 | 0.0004 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0094 | 0.0096 | 0.0153 | 0.005 | 0.0032 | 0.0083 | 0.0149 | 0.0287 | 0.0203 | 0.0164 | 0.0081 | 0.0139 | 0.0127 | 0.0132 | 0.0183 | 0.0211 | 0.0095 | 0.0131 |
Endosome | 0.0251 | 0.0479 | 0.0325 | 0.0256 | 0.0128 | 0.0218 | 0.0559 | 0.055 | 0.0572 | 0.039 | 0.099 | 0.0336 | 0.0455 | 0.064 | 0.0533 | 0.0177 | 0.0905 | 0.0337 |
Golgi | 0.0044 | 0.0033 | 0.0032 | 0.0031 | 0.0028 | 0.0017 | 0.0157 | 0.0027 | 0.0086 | 0.0027 | 0.009 | 0.0027 | 0.007 | 0.0029 | 0.0034 | 0.0031 | 0.0222 | 0.003 |
Lipid Particles | 0.0278 | 0.0308 | 0.0267 | 0.0149 | 0.0104 | 0.0217 | 0.0337 | 0.0228 | 0.0196 | 0.017 | 0.0259 | 0.0161 | 0.0274 | 0.0294 | 0.0135 | 0.0123 | 0.028 | 0.0157 |
Mitochondria | 0.0064 | 0.0016 | 0.0022 | 0.0009 | 0.0074 | 0.0007 | 0.0196 | 0.001 | 0.0058 | 0.0032 | 0.0367 | 0.0021 | 0.0097 | 0.0003 | 0.0008 | 0.0017 | 0.0041 | 0.0081 |
None | 0.6885 | 0.6227 | 0.5903 | 0.679 | 0.7318 | 0.6775 | 0.491 | 0.3697 | 0.3936 | 0.3756 | 0.3156 | 0.3744 | 0.5263 | 0.5654 | 0.5151 | 0.4665 | 0.4464 | 0.5472 |
Nuclear Periphery | 0.0035 | 0.0019 | 0.0027 | 0.0016 | 0.0006 | 0.0015 | 0.0052 | 0.0043 | 0.006 | 0.0081 | 0.0027 | 0.0025 | 0.0058 | 0.0017 | 0.0037 | 0.0032 | 0.0049 | 0.0023 |
Nucleolus | 0.002 | 0.0009 | 0.0009 | 0.0006 | 0.0002 | 0.0004 | 0.0026 | 0.0013 | 0.0013 | 0.0002 | 0.0002 | 0.0005 | 0.0011 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.0003 |
Nucleus | 0.0024 | 0.0012 | 0.0015 | 0.0011 | 0.0008 | 0.001 | 0.0026 | 0.0024 | 0.0051 | 0.0019 | 0.0011 | 0.003 | 0.0034 | 0.001 | 0.0016 | 0.0014 | 0.0018 | 0.0012 |
Peroxisomes | 0.0043 | 0.0009 | 0.0037 | 0.0041 | 0.0202 | 0.0053 | 0.0171 | 0.0015 | 0.0136 | 0.0258 | 0.0018 | 0.0021 | 0.0076 | 0.0012 | 0.0057 | 0.0133 | 0.0035 | 0.0023 |
Punctate Nuclear | 0.0039 | 0.003 | 0.0028 | 0.0045 | 0.0011 | 0.0065 | 0.0053 | 0.0034 | 0.0056 | 0.0026 | 0.0047 | 0.0047 | 0.0099 | 0.0026 | 0.0047 | 0.0028 | 0.007 | 0.004 |
Vacuole | 0.0081 | 0.0048 | 0.0054 | 0.0035 | 0.0016 | 0.0025 | 0.0129 | 0.0082 | 0.005 | 0.0067 | 0.0029 | 0.0031 | 0.0047 | 0.0046 | 0.005 | 0.0016 | 0.0051 | 0.0034 |
Vacuole Periphery | 0.0008 | 0.0005 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0046 | 0.0005 | 0.0012 | 0.0025 | 0.0016 | 0.0006 | 0.0008 | 0.0003 | 0.0005 | 0.0003 | 0.0011 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.1925 | 4.6418 | 4.0185 | 8.4359 | 5.0492 | 7.7844 | 5.4755 | 7.1272 | 9.3698 | 9.524 |
Translational Efficiency | 1.4771 | 1.3564 | 1.6893 | 0.7607 | 0.9935 | 1.0384 | 0.9038 | 0.8388 | 0.7188 | 0.6598 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|