Standard name
Human Ortholog
Description Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.05 0.07 0.08 0.11 0.07 0.1 0.06 0.08 0.13 0.18 0.17 0.07 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0.11 0.16 0.13 0.27 0.16 0.55 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.95 0.97 0.92 0.92 0.84 0.79 0.82 0.81 0.77 0.73 0.82 0.87 0.79 0.82 0.87 0.83 0.83 0.88 0.92 0.89 0.79 0.72 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0.06 0.06 0.06 0 0.07 0.07 0.15 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 5 1 0 2 3 3 0 0 0 1 0 0 0 3 1 1 2 0
Bud Neck 0 0 0 0 0 2 1 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3 4 1 1 2 3 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 2 2 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 9 3 16 10 21 33 35 12 21 18 16 14 30 38 47 56 63 0 1 1 0 2 0
Endoplasmic Reticulum 0 1 1 0 0 0 1 0 0 1 1 0 0 3 1 20 13 17 0 0 1 0 0 0
Endosome 0 1 0 0 1 1 1 0 0 3 0 0 0 0 2 5 7 7 2 3 0 0 0 4
Golgi 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 2 2 1 1 0 0 1 0
Mitochondria 23 6 0 3 6 33 63 41 45 33 154 52 0 1 2 28 18 21 2 2 1 1 4 4
Nucleus 216 344 192 176 194 248 312 258 134 164 206 170 95 132 182 608 493 529 202 345 182 74 110 93
Nuclear Periphery 2 2 0 0 1 3 1 1 0 1 0 0 0 0 1 1 2 4 1 0 0 0 0 0
Nucleolus 2 0 0 0 1 2 4 2 7 4 8 3 2 0 0 4 1 2 4 1 0 2 2 9
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 0 0 2 2 1 1 4 0 2 1 1 1 5 6 6 0 1 1 1 1 2
Vac/Vac Membrane 0 9 1 1 3 12 12 5 2 7 3 13 0 4 4 36 36 41 13 13 14 6 23 34
Unique Cell Count 228 364 197 191 210 296 393 313 165 212 282 207 109 167 222 699 592 638 230 377 206 94 153 157
Labelled Cell Count 248 372 200 198 217 328 442 350 203 243 398 263 113 171 231 755 634 693 230 377 206 94 153 157


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.8 6.2 6.2 6.4 5.3 5.5 5.7 4.9 5.4 4.7 5.1 6.6 7.2 6.8 7.2 7.3 6.7 5.3 6.6 6.8
Std Deviation (1e-4) 0.8 1.0 0.7 1.5 1.4 1.3 1.7 1.4 1.2 1.7 1.2 1.4 1.5 2.0 1.8 1.3 1.4 1.4 1.1 1.8 1.6
Intensity Change (Log2) – – – -0.0 0.05 -0.23 -0.17 -0.11 -0.34 -0.21 -0.41 -0.28 0.1 0.22 0.14 0.22 0.25 0.12 -0.23 0.1 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.1 1.9 2.8 3.3 4.0 2.7 3.6 2.6 2.9 4.1 5.4 5.4 2.8 3.7 3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 5.9 5.3 7.8 5.8 0 7.5 0 0 0 2.9 0 0
Nucleus -2.4 -2.3 -4.8 -5.9 -5.1 -5.1 -6.0 -7.0 -5.0 -3.6 -5.6 -5.1 -4.2 -5.1 -5.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.4 0 0 0 0 0 3.2 0 0 0 2.9 3.2 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1889 2.0569 2.2944 3.7781 3.1346 3.4952 1.3225 1.3589 1.5462 0.5351 2.3882 1.0805 1.6495 1.6865 1.4919 1.572 1.1657 1.4285
Actin 0.0146 0.0008 0.0079 0.0184 0.0079 0.0028 0.0261 0 0.0082 0 0.0605 0.0008 0.0129 0 0.0018 0.0294 0 0.0005
Bud 0.0041 0.0062 0.0024 0.0004 0.0006 0.0002 0.0003 0 0.0004 0 0.0016 0.0006 0.0004 0 0.0003 0.0005 0 0
Bud Neck 0.0016 0.0002 0.0008 0.0003 0.0033 0.0012 0.0009 0.0001 0.0006 0 0.0046 0.0008 0.002 0 0.0009 0.0002 0.0001 0.0005
Bud Periphery 0.0031 0.0017 0.0034 0.0005 0.0013 0.0011 0.0008 0 0.0008 0 0.0034 0.0006 0.0009 0 0.0009 0.0006 0 0.0001
Bud Site 0.0071 0.002 0.0128 0.0007 0.0014 0.0004 0.0025 0 0.0013 0 0.0045 0.0007 0.0022 0.0001 0.0026 0.0014 0 0
Cell Periphery 0.001 0.0005 0.0003 0.0003 0.0002 0.0003 0.0003 0 0.0001 0 0.0006 0 0.0003 0 0.0002 0 0 0
Cytoplasm 0.0068 0.0006 0.018 0.0114 0.003 0.0001 0.013 0.0006 0.0046 0 0.0014 0.0013 0.0078 0.0002 0.0003 0.0006 0.0003 0.0004
Cytoplasmic Foci 0.0632 0.0044 0.0213 0.0045 0.0453 0.0075 0.0087 0 0.001 0 0.013 0.0023 0.0124 0 0.0038 0.0042 0 0.0002
Eisosomes 0.0007 0 0.0002 0.0001 0.0002 0.0003 0.0006 0 0.0001 0 0.0009 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0079 0 0.004 0.005 0.002 0.0005 0.0044 0.0001 0.0014 0 0.0098 0.0005 0.0035 0 0.0005 0.0003 0 0.0001
Endosome 0.0373 0.0004 0.0171 0.0094 0.0492 0.0009 0.0234 0.0008 0.0111 0 0.0706 0.0037 0.016 0 0.012 0.0031 0.0001 0.0014
Golgi 0.0082 0.0005 0.0048 0.0015 0.0148 0.0003 0.0055 0 0.0016 0 0.0443 0.0007 0.0034 0 0.0042 0.003 0 0.0004
Lipid Particles 0.0333 0.0045 0.0087 0.0019 0.0076 0.0026 0.0178 0 0.0013 0 0.1008 0.0004 0.0065 0 0.0128 0.0017 0 0.0007
Mitochondria 0.0204 0.0068 0.0279 0.0657 0.052 0.0247 0.0223 0.0001 0.0019 0 0.0304 0.0008 0.0123 0.0001 0.0083 0.0014 0.0003 0.0006
None 0.0224 0.0002 0.0026 0.0013 0.0015 0.0002 0.006 0.0003 0.0077 0 0.003 0.0004 0.002 0 0.0003 0.0003 0.0004 0.0036
Nuclear Periphery 0.0428 0.0039 0.0166 0.0151 0.0114 0.0061 0.0122 0.0049 0.0116 0.0001 0.044 0.0097 0.0138 0.0016 0.0039 0.002 0.0092 0.0032
Nucleolus 0.0077 0.0114 0.0128 0.0059 0.0204 0.0367 0.0067 0.0053 0.0102 0.0052 0.0097 0.0103 0.0099 0.0054 0.0083 0.0029 0.0084 0.0142
Nucleus 0.6786 0.9399 0.8294 0.7809 0.7126 0.9084 0.8336 0.9865 0.9251 0.9945 0.5503 0.9625 0.8666 0.9586 0.9271 0.94 0.9799 0.9569
Peroxisomes 0.0136 0.0053 0.0031 0.0047 0.0092 0.0027 0.0063 0 0.0004 0 0.0268 0.0011 0.0019 0 0.0027 0.006 0 0.0001
Punctate Nuclear 0.0116 0.0049 0.0023 0.0684 0.0519 0.0007 0.0036 0.0006 0.0038 0.0001 0.0099 0.0018 0.019 0.0338 0.0053 0.0024 0.0009 0.0167
Vacuole 0.0098 0.0028 0.0021 0.0014 0.0026 0.0012 0.0028 0.0003 0.0053 0 0.0043 0.0007 0.003 0.0001 0.0027 0.0001 0.0001 0.0001
Vacuole Periphery 0.0042 0.0032 0.0016 0.0021 0.0016 0.0012 0.0024 0.0002 0.0015 0 0.0055 0.0003 0.003 0 0.0011 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.9087 5.6884 7.1758 9.5758 9.0754 7.6318 9.4901 9.8989 10.5369 10.3522
Translational Efficiency 0.8806 0.9686 0.9865 0.7318 0.8276 1.215 0.9665 0.7643 0.6852 0.8088

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2089 481 1233 206 1704 1639 609 149 3793 2120 1842 355

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 665.55 781.49 996.75 980.78 744.03 749.09 878.08 955.40 700.81 756.44 957.52 970.13
Standard Deviation 92.20 111.41 128.81 140.35 99.98 112.03 159.16 119.27 103.43 112.71 150.32 132.51
Intensity Change Log 2 — 0.231680 0.582685 0.559383 — 0.009778 0.238992 0.360744 — 0.118813 0.411494 0.457948

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000043 0.000580 0.000744 0.002871 0.000107 0.000511 0.000429 0.001245 0.000072 0.000527 0.000640 0.002188
Bud Neck 0.002976 0.005766 0.014463 0.021577 0.003397 0.006429 0.003976 0.023597 0.003165 0.006279 0.010996 0.022425
Bud Site 0.001117 0.001435 0.002069 0.036020 0.000851 0.002100 0.000615 0.012277 0.000997 0.001949 0.001589 0.026054
Cell Periphery 0.000208 0.000120 0.000068 0.000261 0.000171 0.000155 0.000223 0.000089 0.000192 0.000147 0.000119 0.000189
Cytoplasm 0.037177 0.014442 0.003703 0.017191 0.014869 0.016985 0.021870 0.017823 0.027155 0.016408 0.009709 0.017456
Cytoplasmic Foci 0.003284 0.001898 0.000106 0.001191 0.001090 0.004710 0.001368 0.006493 0.002298 0.004072 0.000524 0.003416
Eisosomes 0.000015 0.000023 0.000026 0.000037 0.000012 0.000023 0.000041 0.000030 0.000014 0.000023 0.000031 0.000034
Endoplasmic Reticulum 0.001320 0.007625 0.005235 0.008784 0.002622 0.004624 0.003607 0.005514 0.001905 0.005305 0.004697 0.007411
Endosome 0.000508 0.000648 0.000221 0.004442 0.000280 0.001967 0.000279 0.008157 0.000406 0.001668 0.000240 0.006001
Golgi 0.000110 0.000206 0.000062 0.013206 0.000074 0.000793 0.000056 0.001885 0.000094 0.000659 0.000060 0.008454
Lipid Particles 0.000814 0.000684 0.000111 0.000537 0.000585 0.002777 0.000369 0.000836 0.000711 0.002302 0.000196 0.000663
Mitochondria 0.001003 0.001601 0.001198 0.001907 0.002208 0.007181 0.000587 0.006750 0.001544 0.005915 0.000996 0.003940
Mitotic Spindle 0.001023 0.000994 0.001386 0.019180 0.001524 0.008439 0.001212 0.018861 0.001248 0.006750 0.001328 0.019046
None 0.021128 0.006372 0.001550 0.011974 0.005840 0.007219 0.024751 0.013225 0.014260 0.007027 0.009221 0.012499
Nuclear Periphery 0.001295 0.000786 0.000593 0.001300 0.000800 0.001971 0.000478 0.000898 0.001073 0.001702 0.000555 0.001131
Nuclear Periphery Foci 0.000284 0.000463 0.000093 0.004540 0.000135 0.000734 0.000163 0.000222 0.000217 0.000672 0.000116 0.002728
Nucleolus 0.005599 0.007632 0.004908 0.003676 0.003790 0.007918 0.006200 0.005470 0.004786 0.007853 0.005335 0.004429
Nucleus 0.920705* 0.945796* 0.961123* 0.808792* 0.959566* 0.917928* 0.931655* 0.865209* 0.938163* 0.924251* 0.951380* 0.832471*
Peroxisomes 0.000284 0.001008 0.000157 0.000194 0.000193 0.001816 0.000151 0.001328 0.000243 0.001633 0.000155 0.000670
Vacuole 0.000967 0.001674 0.002033 0.035323 0.001568 0.004942 0.001897 0.008631 0.001237 0.004200 0.001988 0.024120
Vacuole Periphery 0.000142 0.000246 0.000151 0.006998 0.000317 0.000779 0.000075 0.001462 0.000221 0.000658 0.000126 0.004674

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.32 -21.47 -4.78 -3.74 -3.19 -2.75 -11.56 -5.97 -3.84 -3.80 -3.90 -23.96 -6.45 -4.81 -4.19
Bud Neck -2.71 -12.72 -5.43 -4.63 -2.15 -3.61 -4.11 -4.15 -3.48 -3.46 -4.71 -13.43 -6.84 -5.79 -3.50
Bud Site -0.87 -2.04 -2.66 -2.58 -2.41 -2.52 -1.19 -2.68 -2.25 -2.57 -2.36 -2.39 -3.41 -3.14 -3.11
Cell Periphery 2.49 4.83 0.71 -2.11 -5.25 0.34 -0.39 1.19 0.86 2.22 1.30 3.41 1.25 -0.38 -2.99
Cytoplasm 5.57 13.58 6.62 0.63 -3.97 -1.29 -0.93 -0.94 -0.26 -0.26 4.72 10.48 4.08 0.50 -3.25
Cytoplasmic Foci 1.33 6.31 2.57 0.54 -1.67 -4.62 0.27 -0.64 1.31 -0.70 -2.63 5.81 0.76 2.40 -1.48
Eisosomes -3.52 -10.58 -8.44 -6.55 -6.11 -6.02 -9.30 -6.43 -4.27 -1.05 -6.49 -14.26 -10.55 -7.92 -5.83
Endoplasmic Reticulum -6.51 -18.60 -9.78 -3.17 -5.55 -5.83 -6.67 -6.14 -4.16 -3.58 -10.14 -17.83 -11.44 -6.70 -6.50
Endosome -0.82 1.64 -2.58 -2.32 -2.96 -3.79 -1.82 -1.88 -0.86 -1.82 -3.58 1.14 -2.97 -1.35 -3.10
Golgi -0.94 0.85 -1.23 -1.20 -1.24 -3.15 -0.19 -1.40 0.05 -1.39 -3.17 0.64 -1.39 -1.06 -1.40
Lipid Particles 0.38 4.26 -0.42 -0.72 -3.23 -3.67 1.01 -0.47 3.46 -1.72 -3.57 3.72 -0.65 3.22 -3.77
Mitochondria -1.32 -2.15 -4.92 -2.04 -4.24 -3.33 1.82 -0.88 1.87 -2.01 -4.13 0.81 -2.08 2.48 -2.91
Mitotic Spindle -0.49 -0.45 -3.33 -3.30 -3.31 -3.95 -0.24 -2.17 -1.22 -2.14 -4.19 -0.38 -3.95 -3.09 -3.92
None 6.34 10.61 6.42 0.09 -4.14 -1.08 -3.89 -1.58 -0.90 2.56 5.22 4.52 4.18 -0.45 -0.34
Nuclear Periphery 1.21 1.34 -3.37 -6.23 -6.66 -3.78 0.69 -3.84 0.98 -4.32 -2.15 1.42 -4.56 -2.00 -7.76
Nuclear Periphery Foci -1.45 2.46 -2.41 -2.12 -2.56 -3.72 -1.67 -2.55 0.68 -1.68 -3.57 1.12 -2.68 -1.87 -2.77
Nucleolus -1.90 -0.07 1.64 2.67 1.56 -4.69 -2.77 -0.31 2.78 1.41 -4.44 -1.75 0.97 4.13 2.04
Nucleus -2.56 -7.41 5.86 6.65 7.76 7.62 4.38 5.28 2.71 3.72 3.60 -2.77 7.86 6.38 8.62
Peroxisomes -1.62 1.52 -0.56 1.45 -1.80 -4.93 0.72 -1.41 2.90 -1.53 -5.07 1.62 -1.53 3.95 -2.12
Vacuole -1.85 -4.30 -5.22 -4.95 -4.98 -3.74 -2.81 -3.67 -3.02 -3.31 -4.51 -4.80 -6.28 -5.61 -5.92
Vacuole Periphery -1.50 -0.61 -1.72 -1.62 -1.70 -1.85 1.21 -1.05 0.17 -1.86 -2.80 0.93 -1.90 -1.25 -2.04
Standard name
Human Ortholog
Description Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cwc22

Cwc22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cwc22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available