Standard name
Human Ortholog
Description Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.91 – 1.0 0.89 0.8 0.84 0.86 0.83 0.87 0.93 0.88 0.99 0.99 0.98 0.92 0.93 0.91 0.49 0.54 0.52 0.62 0.67 0.67
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.06 – 0 0 0 0.07 0 0.06 0 0.07 0 0 0 0 0 0 0 0.12 0.06 0.26 0.09 0.09 0.08
Nucleus 0 0 – 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0.15 0.18 0.13 0.18 0.17 0.15 0 0.11 0 0 0 0.08 0.07 0.07 0.24 0.32 0.18 0.22 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 0 0 7 8
Bud 3 1 – 0 2 3 3 2 1 1 4 2 0 0 0 1 0 1 2 0 0 1 5 7
Bud Neck 0 0 – 2 0 1 1 0 1 0 0 1 0 0 0 0 1 2 6 1 0 0 3 6
Bud Site 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 – 0 0 0 0 3 0 1 1 0 0 0 0 1 2 2 0 0 0 0 2 4
Cytoplasm 251 98 – 45 204 104 172 169 131 167 152 143 302 373 384 231 240 280 147 63 13 79 214 211
Endoplasmic Reticulum 2 2 – 0 2 4 0 0 2 0 1 1 3 2 1 19 16 18 2 0 0 0 3 3
Endosome 1 0 – 0 3 1 0 0 0 0 0 0 0 0 2 2 2 4 18 2 0 1 3 5
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 1 3 0 0 0 0 5 1
Mitochondria 35 6 – 0 1 5 14 6 9 8 11 6 0 0 2 4 4 6 36 6 6 11 30 25
Nucleus 0 2 – 0 2 11 8 7 7 7 1 4 0 0 1 2 2 1 0 1 0 0 1 1
Nuclear Periphery 2 2 – 0 0 1 4 1 0 2 2 2 1 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 – 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 3 0 0 0 7 4
Vac/Vac Membrane 3 2 – 1 35 24 27 36 27 29 5 18 0 0 0 21 19 22 71 37 4 27 24 20
Unique Cell Count 280 108 45 228 130 205 196 157 192 163 163 305 375 391 252 258 307 301 117 27 129 323 314
Labelled Cell Count 301 114 48 251 154 230 224 178 215 177 179 306 376 392 281 288 339 301 117 27 129 323 314


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.1 – 4.6 5.6 4.6 4.5 4.9 4.4 4.5 4.4 4.5 5.3 5.3 5.4 5.6 6.3 5.8 5.0 5.3 5.8
Std Deviation (1e-4) 0.6 0.8 – 2.1 1.4 1.2 1.2 1.2 1.1 0.9 1.5 1.0 1.3 1.4 1.9 1.2 3.0 1.6 1.7 1.8 2.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7853 -0.6661 -0.8682 -0.4337 -0.5218 -0.9234 0.2096 0.0722 -0.1155 0.3022 0.1794 -0.1278 -0.8987 -0.8787 -1.0545 -0.8127 -1.3151 -0.9764
Actin 0.0294 0.001 0.0158 0.011 0.0448 0.0005 0.0294 0.0006 0.0072 0.0263 0.0333 0.0078 0.0197 0.0002 0.0071 0.0244 0.0001 0.0005
Bud 0.001 0.0074 0.0022 0.0461 0.0002 0.0001 0.0004 0.001 0.0001 0.0007 0.0011 0.0019 0.0008 0.0001 0.0018 0.018 0.0001 0.0001
Bud Neck 0.007 0.0002 0.0005 0.0032 0.0006 0.0157 0.0123 0.0003 0.0001 0.0007 0.0086 0.001 0.0032 0.0001 0.0005 0.0002 0 0.0022
Bud Periphery 0.0012 0.0018 0.0011 0.0093 0.0003 0.0004 0.0007 0.0003 0.0002 0.0011 0.0012 0.0009 0.0014 0.0001 0.0024 0.0031 0.0001 0.0003
Bud Site 0.0016 0.017 0.0054 0.0049 0.0006 0 0.0161 0.0086 0.0004 0.0042 0.011 0.0004 0.0066 0.0004 0.008 0.0012 0 0.0002
Cell Periphery 0.0002 0.0006 0.0002 0.0005 0.0001 0 0.001 0.0002 0.0001 0.0002 0.0003 0.0001 0.0003 0.0001 0.0003 0.0002 0 0.0001
Cytoplasm 0.0204 0.0225 0.0291 0.0214 0.0158 0.062 0.0279 0.0577 0.0468 0.0297 0.0545 0.0986 0.1073 0.1006 0.138 0.1763 0.1245 0.2003
Cytoplasmic Foci 0.0291 0.0084 0.0081 0.0138 0.0219 0.013 0.02 0.0129 0.0151 0.0316 0.021 0.0136 0.0416 0.0099 0.0334 0.0149 0.0099 0.0332
Eisosomes 0.0004 0.0003 0.0004 0.0003 0.0003 0.0001 0.0007 0.0002 0.0001 0.0003 0.0005 0.0001 0.0004 0.0003 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.003 0.0005 0.0012 0.0021 0.003 0.0004 0.0022 0.0014 0.0017 0.0023 0.0011 0.001 0.0046 0.0011 0.0021 0.001 0.0008 0.0022
Endosome 0.0129 0.0016 0.0013 0.0148 0.0188 0.0082 0.0158 0.0059 0.0106 0.065 0.0219 0.0059 0.0541 0.0081 0.0354 0.0156 0.0043 0.023
Golgi 0.0031 0.0003 0.0009 0.0056 0.0064 0.0001 0.0051 0.0004 0.001 0.0144 0.0149 0.0005 0.0055 0.0002 0.0036 0.0019 0.0001 0.0005
Lipid Particles 0.0182 0.0008 0.0006 0.0518 0.0118 0.0006 0.0166 0.0007 0.0045 0.0185 0.0275 0.0007 0.0147 0.0007 0.0059 0.0014 0.0005 0.0022
Mitochondria 0.0024 0.0013 0.0015 0.0141 0.0016 0.0005 0.0015 0.0005 0.0012 0.0226 0.035 0.001 0.0042 0.0004 0.0051 0.0015 0.0007 0.0013
None 0.8149 0.9217 0.9152 0.7328 0.845 0.8906 0.8154 0.8944 0.8873 0.7471 0.7174 0.8492 0.6754 0.8574 0.7099 0.7099 0.8529 0.6961
Nuclear Periphery 0.0163 0.0008 0.0012 0.0134 0.0082 0.0007 0.0069 0.0011 0.0006 0.0036 0.0009 0.0011 0.0095 0.0007 0.0023 0.0014 0.0007 0.007
Nucleolus 0.0026 0.0014 0.0014 0.0085 0.0007 0.0003 0.0009 0.0006 0.0004 0.0008 0.0054 0.0008 0.0017 0.0005 0.0013 0.0007 0.0002 0.0006
Nucleus 0.0068 0.0024 0.0029 0.0253 0.0014 0.0009 0.0031 0.0029 0.0008 0.0017 0.0135 0.0033 0.0058 0.002 0.0027 0.0056 0.0009 0.0074
Peroxisomes 0.0084 0.0057 0.0009 0.0125 0.0035 0.0004 0.0087 0.0004 0.0036 0.008 0.0241 0.0005 0.0154 0.0002 0.0137 0.0044 0.0001 0.0013
Punctate Nuclear 0.018 0.0031 0.0094 0.0032 0.0135 0.0008 0.013 0.0035 0.0008 0.0045 0.0031 0.0059 0.0076 0.0086 0.0034 0.0011 0.0006 0.0026
Vacuole 0.0024 0.001 0.0006 0.0032 0.0008 0.004 0.0017 0.006 0.0161 0.0135 0.0029 0.0053 0.0176 0.0077 0.0207 0.0158 0.0027 0.0175
Vacuole Periphery 0.0008 0.0002 0.0002 0.0019 0.0006 0.0005 0.0007 0.0004 0.0013 0.0029 0.0007 0.0004 0.0028 0.0006 0.0022 0.0013 0.0004 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1006.183 1073.2862 1039.6255 857.0846 695.563 1017.3329 1091.8156 988.9671 1013.4425 796.1159
Translational Efficiency 1.2693 1.2395 1.2842 1.4313 1.3456 1.285 1.4733 1.4535 1.3793 1.3617

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2050 1282 1944 1709 1528 2100 468 1254 3578 3382 2412 2963

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 707.73 770.55 800.77 804.77 614.44 726.21 863.87 728.72 667.89 743.02 813.01 772.58
Standard Deviation 75.55 91.47 90.26 105.86 72.27 87.59 90.27 127.51 87.35 91.64 93.65 121.48
Intensity Change Log 2 — 0.122689 0.178189 0.185377 — 0.241115 0.491542 0.246092 — 0.178935 0.332303 0.213911

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000309 0.000435 0.000119 0.000596 0.000444 0.000607 0.000191 0.001233 0.000367 0.000542 0.000133 0.000866
Bud Neck 0.026664 0.029100 0.006489 0.005028 0.044013 0.042851 0.001765 0.007571 0.034073 0.037638 0.005572 0.006104
Bud Site 0.004626 0.014438 0.008222 0.023519 0.006434 0.017756 0.005039 0.034728 0.005398 0.016498 0.007604 0.028263
Cell Periphery 0.000160 0.000157 0.000142 0.000223 0.000364 0.000187 0.000176 0.000243 0.000247 0.000176 0.000149 0.000231
Cytoplasm 0.451388* 0.393322* 0.412403* 0.487669* 0.410633* 0.395619* 0.321065* 0.573577* 0.433983* 0.394748* 0.394680* 0.524027*
Cytoplasmic Foci 0.235084* 0.202661* 0.011777 0.040718 0.257854* 0.217974* 0.027501 0.031863 0.244808* 0.212170* 0.014828 0.036971
Eisosomes 0.000382 0.000167 0.000026 0.000071 0.000953 0.000231 0.000084 0.000057 0.000626 0.000207 0.000037 0.000065
Endoplasmic Reticulum 0.000957 0.001176 0.002505 0.002750 0.001769 0.000932 0.002953 0.001441 0.001304 0.001024 0.002592 0.002196
Endosome 0.023587 0.051132 0.002486 0.007166 0.043605 0.037761 0.004832 0.004209 0.032136 0.042829 0.002941 0.005914
Golgi 0.003116 0.003953 0.000062 0.001960 0.008495 0.005750 0.000079 0.001055 0.005413 0.005068 0.000065 0.001577
Lipid Particles 0.011234 0.007219 0.001049 0.002720 0.017729 0.007474 0.003213 0.001700 0.014008 0.007377 0.001469 0.002288
Mitochondria 0.003980 0.004283 0.000701 0.001525 0.007257 0.008204 0.000326 0.001136 0.005379 0.006718 0.000629 0.001360
Mitotic Spindle 0.002081 0.005167 0.003158 0.011630 0.000804 0.005229 0.004530 0.010906 0.001536 0.005206 0.003424 0.011323
None 0.001015 0.002268 0.004538 0.002928 0.003093 0.002477 0.007219 0.005715 0.001902 0.002397 0.005059 0.004107
Nuclear Periphery 0.000330 0.000603 0.002106 0.001652 0.000239 0.000414 0.001854 0.000868 0.000291 0.000486 0.002057 0.001320
Nuclear Periphery Foci 0.000259 0.000484 0.000766 0.002068 0.000265 0.000275 0.001917 0.001503 0.000262 0.000354 0.000990 0.001829
Nucleolus 0.000696 0.001537 0.000597 0.000500 0.000704 0.001319 0.000782 0.000617 0.000699 0.001402 0.000633 0.000550
Nucleus 0.125693 0.072988 0.505211* 0.219149* 0.076822 0.092826 0.510494* 0.206200* 0.104822 0.085306 0.506236* 0.213669*
Peroxisomes 0.007685 0.014273 0.000218 0.000668 0.010329 0.013523 0.000607 0.001229 0.008814 0.013807 0.000294 0.000905
Vacuole 0.100008 0.191499 0.037194 0.185460 0.106201 0.146233 0.105169 0.112822 0.102653 0.163392 0.050383 0.154718
Vacuole Periphery 0.000744 0.003138 0.000229 0.002001 0.001994 0.002361 0.000204 0.001326 0.001278 0.002655 0.000224 0.001716

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.93 1.21 -0.95 -0.07 -4.05 -1.27 3.33 -0.89 -0.49 -1.45 -1.67 2.57 -1.21 -0.30 -2.44
Bud Neck -0.87 11.84 14.44 12.88 2.00 0.42 18.61 15.73 17.02 -4.41 -1.77 19.49 21.22 21.73 0.63
Bud Site -7.20 -2.35 -5.90 -0.59 -3.77 -7.91 1.33 -5.37 -1.50 -5.44 -10.78 -1.45 -7.89 -1.12 -6.13
Cell Periphery 0.43 1.12 -0.84 -1.00 -1.53 9.51 9.86 6.43 0.03 -0.56 6.21 5.76 4.65 -0.32 -1.73
Cytoplasm 5.84 8.53 4.61 -1.63 -3.48 1.80 9.56 -6.11 -7.83 -13.53 6.10 10.38 -0.63 -6.10 -10.06
Cytoplasmic Foci 5.26 49.14 43.01 30.69 -11.53 5.99 42.47 43.46 40.67 -0.77 7.21 66.88 60.77 50.80 -11.60
Eisosomes 7.73 15.05 13.22 5.41 -15.58 8.84 10.88 11.30 7.08 4.80 10.65 15.96 15.26 8.55 -10.45
Endoplasmic Reticulum -1.22 -5.30 -12.43 -11.31 -0.09 3.02 -5.75 -1.69 -8.07 5.68 1.77 -5.71 -9.41 -14.57 1.48
Endosome -8.33 14.54 12.73 15.77 -4.54 2.08 15.88 16.65 20.51 2.76 -5.10 21.69 20.79 25.36 -2.60
Golgi -1.15 10.81 4.57 4.31 -2.41 2.75 10.98 9.99 7.28 -2.67 0.73 14.53 10.59 8.02 -3.22
Lipid Particles 4.72 16.15 13.84 7.45 -5.67 9.74 14.12 16.08 12.78 4.76 10.34 22.26 21.12 14.24 -2.84
Mitochondria -0.26 5.29 4.42 5.57 -1.19 -0.77 8.13 7.29 8.95 -1.90 -1.75 9.28 8.29 10.26 -1.46
Mitotic Spindle -2.13 -2.38 -4.45 -2.35 -2.80 -4.08 -1.09 -4.76 -1.73 -1.98 -4.04 -3.15 -6.33 -2.99 -3.41
None -3.41 -7.87 -5.88 -0.71 3.55 0.79 -1.39 -1.94 -2.83 0.49 -1.12 -5.10 -4.28 -2.26 2.07
Nuclear Periphery -4.06 -17.10 -19.98 -14.39 1.53 -3.06 -19.14 -11.36 -8.76 5.17 -4.48 -20.57 -22.63 -17.54 4.29
Nuclear Periphery Foci -4.61 -6.76 -14.58 -11.97 -5.43 -0.25 -7.87 -5.35 -5.31 0.93 -3.23 -9.43 -11.89 -11.12 -3.56
Nucleolus -3.03 -0.51 3.54 4.04 3.38 -4.79 -0.27 1.62 5.31 1.59 -5.17 -0.64 3.56 6.93 3.30
Nucleus 12.08 -53.26 -18.18 -26.72 33.09 -4.39 -31.76 -23.38 -20.66 17.48 6.31 -64.65 -28.94 -32.91 38.01
Peroxisomes -4.60 12.13 11.74 11.12 -3.45 -2.67 13.24 12.06 12.82 -0.80 -5.51 18.08 16.82 17.29 -2.21
Vacuole -14.34 12.24 -24.44 -8.14 -31.75 -7.90 -4.98 -11.83 -6.45 -3.71 -15.79 8.07 -26.18 -12.64 -30.18
Vacuole Periphery -8.69 8.76 -0.90 4.76 -2.50 -1.42 9.15 3.47 4.75 -1.90 -8.19 11.50 1.46 6.37 -3.10
Standard name
Human Ortholog
Description Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall
Localization
Cell Percentages cytoplasm (19%), vacuole (9%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bgl2

Bgl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bgl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available