Standard name | |
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Human Ortholog | |
Description | Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 9 | 8 | 1 | 2 | – | 7 | 1 | 3 | 4 | 4 | 2 | 7 | 14 | 0 | 2 | 0 | 0 | 2 | 7 | 2 | 4 | 8 |
Bud | 0 | 1 | 0 | 1 | 0 | 2 | – | 1 | 1 | 4 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 8 | 0 | 0 | 0 | – | 3 | 1 | 0 | 2 | 3 | 4 | 14 | 29 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 4 | 15 | 10 | 17 | 10 | – | 54 | 11 | 8 | 15 | 20 | 7 | 23 | 35 | 5 | 16 | 6 | 0 | 0 | 2 | 0 | 0 | 4 |
Endoplasmic Reticulum | 45 | 65 | 79 | 15 | 3 | 7 | – | 32 | 17 | 18 | 20 | 25 | 123 | 374 | 79 | 0 | 3 | 7 | 76 | 81 | 457 | 306 | 410 | 530 |
Endosome | 0 | 0 | 3 | 4 | 10 | 6 | – | 3 | 4 | 9 | 8 | 8 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 4 | 15 |
Golgi | 3 | 0 | 36 | 14 | 6 | 1 | – | 23 | 6 | 7 | 1 | 12 | 12 | 30 | 19 | 0 | 12 | 16 | 0 | 0 | 1 | 3 | 5 | 6 |
Mitochondria | 0 | 1 | 9 | 12 | 7 | 7 | – | 13 | 10 | 23 | 19 | 18 | 0 | 0 | 0 | 2 | 13 | 12 | 0 | 0 | 3 | 4 | 5 | 11 |
Nucleus | 1 | 0 | 2 | 3 | 3 | 20 | – | 25 | 9 | 23 | 29 | 29 | 3 | 2 | 4 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 34 | 19 | 87 | 113 | 107 | 192 | – | 338 | 261 | 249 | 187 | 282 | 142 | 114 | 49 | 4 | 27 | 25 | 7 | 20 | 51 | 155 | 123 | 154 |
Nucleolus | 1 | 0 | 3 | 9 | 41 | 21 | – | 64 | 50 | 31 | 32 | 61 | 1 | 0 | 6 | 0 | 3 | 4 | 0 | 0 | 0 | 2 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 13 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 7 |
Vac/Vac Membrane | 10 | 19 | 163 | 149 | 228 | 205 | – | 355 | 203 | 316 | 178 | 349 | 72 | 34 | 149 | 6 | 63 | 34 | 0 | 3 | 6 | 25 | 34 | 86 |
Unique Cell Count | 64 | 90 | 349 | 303 | 400 | 420 | 787 | 468 | 598 | 421 | 681 | 283 | 454 | 336 | 16 | 119 | 96 | 87 | 111 | 543 | 511 | 598 | 845 | |
Labelled Cell Count | 94 | 113 | 416 | 338 | 440 | 474 | 918 | 575 | 691 | 500 | 814 | 366 | 598 | 391 | 19 | 143 | 108 | 87 | 111 | 543 | 511 | 598 | 845 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.8 | 14.1 | 21.1 | 21.2 | 20.2 | 16.5 | – | 17.2 | 15.9 | 16.0 | 15.8 | 16.4 | 17.5 | 18.6 | 18.5 | 12.8 | 18.8 | 22.7 | 20.6 | 21.0 | 21.8 |
Std Deviation (1e-4) | 2.9 | 2.7 | 4.3 | 4.4 | 4.5 | 3.5 | – | 5.2 | 3.9 | 3.7 | 4.4 | 4.1 | 2.9 | 3.2 | 3.6 | 3.2 | 4.7 | 5.6 | 3.2 | 3.7 | 4.0 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.06 | -0.35 | – | -0.29 | -0.41 | -0.4 | -0.41 | -0.36 | -0.26 | -0.18 | -0.19 | -0.72 | -0.16 | 0.11 | -0.03 | -0.01 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.7 | 0 | 0 | -1.0 | 1.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -0.8 | 0.7 | 3.7 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 0 | -1.5 | – | 1.7 | -1.6 | -2.9 | -0.5 | -1.1 | -1.2 | 0.5 | 3.1 | 0 | 3.5 | 0 |
Endoplasmic Reticulum | -6.4 | -9.6 | -9.2 | – | -9.7 | -8.3 | -9.6 | -7.4 | -9.6 | 5.6 | 16.9 | 0.3 | 0 | -5.0 | -3.4 |
Endosome | 0 | 1.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.7 | -5.2 | -6.5 | – | -5.2 | -5.8 | -6.5 | -6.5 | -6.2 | -2.9 | -1.9 | -2.2 | 0 | -0.1 | 1.7 |
Mitochondria | 1.0 | -0.8 | -0.9 | – | -1.0 | -0.4 | 1.0 | 1.4 | 0.1 | 0 | 0 | 0 | 0 | 3.7 | 0 |
Nucleus | 0 | 0 | 3.5 | – | 2.7 | 0 | 3.0 | 4.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3.4 | 0.6 | 6.0 | – | 5.8 | 8.8 | 5.2 | 5.6 | 5.2 | 6.6 | 0.1 | -3.4 | 0 | -0.5 | 0.2 |
Nucleolus | 2.0 | 5.5 | 3.3 | – | 4.8 | 5.6 | 3.5 | 4.5 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | 2.8 | 0.6 | – | -0.5 | -0.9 | 1.8 | -1.2 | 1.4 | -5.5 | -12.8 | -0.6 | -0.7 | 1.2 | -2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0095 | 0.0002 | 0.0041 | 0.0005 | 0.0119 | 0.0006 | 0.0279 | 0.0004 | 0.0154 | 0.0006 | 0.0188 | 0.0004 | 0.0145 | 0.0017 | 0.0027 | 0.0002 | 0.0004 | 0.0008 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 |
Bud Neck | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0001 | 0.0012 | 0.0001 |
Bud Periphery | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0006 | 0.0002 | 0.0006 | 0.0003 | 0.0013 | 0 | 0.001 | 0 | 0.0003 | 0.0001 | 0.0064 | 0 | 0.0041 | 0 |
Cell Periphery | 0.0004 | 0.0026 | 0.0012 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.002 | 0.0011 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0019 | 0.0003 | 0.0008 | 0.0003 | 0.0002 |
Cytoplasm | 0.004 | 0.0039 | 0.0058 | 0.0014 | 0.0005 | 0.001 | 0.0203 | 0.0004 | 0.001 | 0.0008 | 0.0007 | 0.0002 | 0.0167 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0049 | 0.0224 | 0.0173 | 0.0059 | 0.0041 | 0.006 | 0.0122 | 0.0778 | 0.0265 | 0.015 | 0.0116 | 0.0036 | 0.0003 | 0.0001 | 0.1909 | 0 | 0.0781 | 0.0001 |
Eisosomes | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.1102 | 0.3321 | 0.2091 | 0.4244 | 0.3367 | 0.2091 | 0.1057 | 0.333 | 0.2831 | 0.4548 | 0.425 | 0.1821 | 0.0172 | 0.1325 | 0.0001 | 0.944 | 0.0001 | 0.0073 |
Endosome | 0.0034 | 0.0006 | 0.0076 | 0.0011 | 0.0111 | 0.0026 | 0.0262 | 0.0009 | 0.002 | 0.001 | 0.0093 | 0.0005 | 0.0103 | 0.0024 | 0.0008 | 0.0016 | 0.0009 | 0.0004 |
Golgi | 0.0025 | 0.0003 | 0.0028 | 0.0003 | 0.009 | 0.0009 | 0.0065 | 0.0015 | 0.0017 | 0.0006 | 0.0074 | 0.0008 | 0.0003 | 0.0014 | 0.0037 | 0.0002 | 0.0023 | 0.001 |
Lipid Particles | 0.006 | 0.0098 | 0.0147 | 0.0082 | 0.0147 | 0.0143 | 0.032 | 0.0644 | 0.0595 | 0.0477 | 0.067 | 0.0155 | 0.0001 | 0.0025 | 0.099 | 0.0003 | 0.0658 | 0.0045 |
Mitochondria | 0.0013 | 0.0005 | 0.0023 | 0.0004 | 0.0039 | 0.0014 | 0.0019 | 0.0006 | 0.0004 | 0.0007 | 0.0029 | 0.0001 | 0.0098 | 0.003 | 0.0111 | 0.0001 | 0.0011 | 0.0008 |
None | 0.0052 | 0.0001 | 0.0014 | 0.0001 | 0.0004 | 0.0008 | 0.0093 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.004 | 0.0003 | 0.0014 | 0 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.7694 | 0.4806 | 0.517 | 0.4826 | 0.5348 | 0.6825 | 0.6859 | 0.3548 | 0.4364 | 0.4003 | 0.3225 | 0.7421 | 0.6467 | 0.7304 | 0.0011 | 0.0445 | 0.0009 | 0.9502 |
Nucleolus | 0.0092 | 0.0213 | 0.0534 | 0.0036 | 0.0025 | 0.0062 | 0.0015 | 0.0422 | 0.0432 | 0.0028 | 0.0301 | 0.0027 | 0.0008 | 0.0001 | 0.153 | 0 | 0.463 | 0.0015 |
Nucleus | 0.0199 | 0.0421 | 0.0381 | 0.0109 | 0.0468 | 0.0162 | 0.0142 | 0.008 | 0.0122 | 0.0276 | 0.0301 | 0.0047 | 0.2633 | 0.0052 | 0.0125 | 0.0009 | 0.0293 | 0.0037 |
Peroxisomes | 0.0003 | 0 | 0.0066 | 0 | 0.0041 | 0.0001 | 0.0005 | 0.0009 | 0.0003 | 0.0001 | 0.0028 | 0 | 0.0001 | 0 | 0.0149 | 0 | 0.0029 | 0 |
Punctate Nuclear | 0.0235 | 0.0582 | 0.093 | 0.0377 | 0.0028 | 0.0404 | 0.019 | 0.0813 | 0.0805 | 0.0174 | 0.0554 | 0.0257 | 0.0046 | 0.0003 | 0.4983 | 0 | 0.3476 | 0.0016 |
Vacuole | 0.0048 | 0.0107 | 0.0051 | 0.0061 | 0.0038 | 0.0046 | 0.0038 | 0.0076 | 0.0053 | 0.0032 | 0.0023 | 0.0014 | 0.002 | 0.009 | 0.0003 | 0.0045 | 0.0004 | 0.0004 |
Vacuole Periphery | 0.0235 | 0.0139 | 0.0187 | 0.0156 | 0.0105 | 0.0118 | 0.0315 | 0.0225 | 0.0294 | 0.0266 | 0.0112 | 0.0199 | 0.0081 | 0.1078 | 0.0006 | 0.0021 | 0.0002 | 0.027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.525 | 64.6412 | 53.2961 | 57.5458 | 59.0301 | 64.2404 | 73.6293 | 60.1784 | 50.4592 | 74.135 |
Translational Efficiency | 1.161 | 1.148 | 0.9316 | 1.0434 | 1.0967 | 1.248 | 0.9274 | 1.0164 | 1.1284 | 0.8944 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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