Standard name
Human Ortholog
Description Protein of unknown function; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0.06 0.09 0.1 0.13 0 0.09 0.09
Bud 0 0 0 0 0.05 0.06 0.06 0.08 0.07 0.09 0.07 0.09 0 0 0 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.57 0.62 0.39 0.34 0.27 0.25 0.22 0.21 0.18 0.16 0.15 0.16 0.76 0.78 0.76 0.59 0.47 0.27 0.23 0.11 0.16 0.14 0.14
Endoplasmic Reticulum 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0.14 0.08 0.27 0.15 0.38 0.09 0.06 0.11 0 0 0 0 0.06 0.06 0.13 0.11 0.2 0.12 0.15 0.2 0.15 0.15 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.2 0.2 0.48 0.31 0.68 0.73 0.71 0.81 0.86 0.86 0.84 0 0 0 0.12 0.15 0.31 0.32 0.29 0.43 0.39 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.13 0.11 0.19 0 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.07 0.1 0.08 0.09 0.07 0.09 0 0 0.09 0.06 0.05 0.06 0.15 0.15 0 0 0.08 0.05 0 0
Vac/Vac Membrane 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.05 0 0.05 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 10 0 1 0 0 0 0 1 0 2 1 56 57 18 7 11 20 18 31 19 44 55
Bud 5 5 8 9 13 15 21 39 21 30 22 32 14 14 12 8 13 4 3 6 5 8 13
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 37 26 23 8 5 4 7 3 9 13 13
Bud Site 0 0 1 0 0 4 3 5 6 5 4 10 0 1 0 0 0
Cell Periphery 1 1 0 2 0 0 1 1 2 1 0 3 3 3 4 0 2 0 0 0 0 2 2
Cytoplasm 104 91 77 72 73 65 72 104 52 54 52 58 631 631 680 134 85 66 43 25 69 72 81
Endoplasmic Reticulum 14 13 2 8 1 3 2 1 3 3 3 1 30 32 11 10 13 2 5 3 6 10 13
Endosome 25 12 53 32 102 23 20 54 11 8 7 5 46 50 113 26 35 30 27 46 66 75 86
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 7 4 9 8 15 23
Mitochondria 38 29 40 101 84 174 240 342 228 288 289 299 2 2 2 27 26 74 59 69 189 205 222
Nucleus 0 0 0 1 1 0 0 7 2 3 0 2 5 5 3 1 1 2 0 0 0 1 0
Nuclear Periphery 0 0 1 0 0 0 1 0 0 0 0 2 0 0 0 1 0 0 0 1 1 1 0
Nucleolus 0 0 0 1 0 1 4 4 3 2 2 5 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 6 0 25 24 52 11 15 31 19 4 9 10 1 0 8 7 1 2 0 8 7 10 18
SpindlePole 7 1 14 14 26 21 29 33 25 14 13 31 53 40 50 34 27 9 2 19 22 23 25
Vac/Vac Membrane 7 0 9 4 14 6 8 14 6 1 7 10 25 22 42 3 6 12 9 4 21 28 28
Unique Cell Count 181 146 196 209 268 256 328 485 282 336 336 356 835 814 894 229 179 242 187 237 437 520 597
Labelled Cell Count 210 164 231 269 366 323 416 636 379 413 410 469 903 883 967 266 227 242 187 237 437 520 597


Ambiguous, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.0 4.8 4.5 4.7 4.0 3.8 4.1 3.8 3.5 3.5 3.5 5.8 5.8 6.0 4.5 4.9 5.8 5.7 5.9 6.2
Std Deviation (1e-4) 0.7 0.7 1.8 1.2 1.8 1.9 1.3 1.5 1.3 1.1 0.9 1.0 0.7 0.7 0.8 0.8 1.0 1.3 0.9 1.2 1.7
Intensity Change (Log2) -0.11 -0.04 -0.27 -0.35 -0.23 -0.34 -0.47 -0.46 -0.45 0.28 0.28 0.32 -0.1 0.03 0.26 0.23 0.29 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.7 3.8 0 0 3.5
Bud 0 0 0 0 0 0 0 0 0 0 0 0 -0.3 1.3
Bud Neck 0 0 0 0 0 0 0 0 0 3.0 2.5 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -2.7 -3.2 -4.3 -4.8 -5.0 -6.0 -6.2 -6.0 9.9 10.5 10.1 4.0 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1.9 2.0 0 2.1 3.1
Endosome 0 0 0 0 0 0 0 0 0 -9.2 -8.7 -5.1 -4.1 -1.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -12.9 -12.7 -13.3 -2.4 -1.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 -8.8 -3.8 -4.6
SpindlePole 0 0 0 0 0 0 0 0 0 -0.4 -1.2 -0.8 2.5 2.5
Vacuole -1.5 0.3 -1.3 -1.3 -1.1 -1.5 0 -1.6 -1.1 -1.1 -1.4 0.1 -2.0 -0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4463 1.2147 1.5262 0.7773 1.1448 1.2465 1.2183 2.1977 2.7081 2.7623 2.54 2.2504 2.2307 2.5097 2.4118 2.5815 1.9549 2.337
Actin 0.0842 0.0094 0.0106 0.0047 0.0269 0.0057 0.0113 0.0088 0.0327 0.0012 0.0278 0.0022 0.0044 0.0081 0.0296 0.0327 0.0154 0.0115
Bud 0.0017 0.0036 0.0106 0.0152 0.0048 0.0027 0.0003 0.0004 0.0054 0.0023 0.0008 0.0001 0.0001 0.0009 0.0047 0.0018 0.0092 0.0003
Bud Neck 0.0031 0.0022 0.0055 0.0323 0.0023 0.0031 0.0269 0.0003 0.0012 0.0003 0.0058 0.0035 0.0014 0.0013 0.0052 0.0068 0.0014 0.008
Bud Periphery 0.0043 0.003 0.0177 0.0188 0.0075 0.006 0.0004 0.0008 0.0103 0.0043 0.001 0.0001 0.0001 0.0009 0.0066 0.0039 0.0181 0.0004
Bud Site 0.0045 0.0254 0.0396 0.0339 0.0322 0.0006 0.0094 0.0011 0.0059 0.0007 0.0226 0.0003 0.0015 0.0266 0.0153 0.0025 0.0182 0.0007
Cell Periphery 0.0009 0.0007 0.0019 0.001 0.0005 0.0004 0.0015 0.0004 0.0011 0.0004 0.0003 0.0001 0.0005 0.0009 0.0008 0.0008 0.0006 0.0002
Cytoplasm 0.1474 0.2477 0.186 0.1486 0.1523 0.2379 0.1519 0.2321 0.1748 0.3194 0.1038 0.2415 0.1199 0.3116 0.2737 0.368 0.2237 0.2586
Cytoplasmic Foci 0.1018 0.1533 0.1505 0.1083 0.1889 0.2027 0.2749 0.1748 0.0835 0.1818 0.1351 0.2164 0.3051 0.1748 0.1208 0.0717 0.1799 0.276
Eisosomes 0.0008 0.0005 0.0004 0.0005 0.0004 0.0001 0.0004 0.0003 0.0009 0.0001 0.0004 0.0001 0.0004 0.0001 0.001 0.0006 0.0004 0.0001
Endoplasmic Reticulum 0.0153 0.0057 0.0048 0.0079 0.004 0.0045 0.003 0.0041 0.006 0.0024 0.0015 0.0012 0.0009 0.0011 0.0041 0.008 0.0037 0.003
Endosome 0.1086 0.1047 0.0883 0.3073 0.1423 0.1323 0.083 0.0689 0.1084 0.111 0.1215 0.0685 0.104 0.051 0.0694 0.1041 0.1786 0.1213
Golgi 0.0125 0.0126 0.019 0.0206 0.0195 0.0138 0.0074 0.0053 0.021 0.0077 0.0292 0.0048 0.025 0.0115 0.0183 0.0187 0.0172 0.023
Lipid Particles 0.027 0.0127 0.0083 0.0086 0.0113 0.0307 0.0307 0.0042 0.0153 0.0022 0.0061 0.0044 0.0189 0.0058 0.008 0.0041 0.0263 0.0091
Mitochondria 0.0177 0.0072 0.0148 0.0165 0.0098 0.0214 0.0012 0.0016 0.0217 0.0064 0.0189 0.0027 0.0041 0.0024 0.0123 0.0397 0.0569 0.0087
None 0.3908 0.3798 0.3931 0.2203 0.3424 0.3022 0.382 0.4824 0.4684 0.3306 0.4946 0.4286 0.3539 0.3868 0.4082 0.3143 0.1788 0.2479
Nuclear Periphery 0.0272 0.0019 0.0055 0.0052 0.007 0.0033 0.0004 0.0007 0.0023 0.0008 0.0004 0.0002 0.0001 0.0002 0.0007 0.0027 0.0014 0.0006
Nucleolus 0.0012 0.001 0.0009 0.0062 0.0013 0.0003 0.0001 0.0002 0.0006 0.0002 0.0003 0.0001 0.0002 0.0002 0.0003 0.0003 0.0005 0.0001
Nucleus 0.0258 0.0031 0.0029 0.003 0.0022 0.001 0.0002 0.0005 0.0013 0.0006 0.0004 0.0003 0.0002 0.001 0.0007 0.0014 0.0012 0.0004
Peroxisomes 0.0102 0.0127 0.0242 0.0176 0.0324 0.0217 0.0119 0.0045 0.0285 0.0122 0.0245 0.0214 0.0562 0.0116 0.0155 0.005 0.0538 0.0246
Punctate Nuclear 0.008 0.0041 0.005 0.0024 0.0054 0.0022 0.0015 0.0007 0.0034 0.0005 0.0014 0.0012 0.0014 0.0004 0.0011 0.001 0.0035 0.0023
Vacuole 0.0051 0.0072 0.0082 0.0149 0.004 0.0052 0.0012 0.0064 0.0052 0.0124 0.0024 0.0017 0.0014 0.0022 0.003 0.0089 0.0092 0.0023
Vacuole Periphery 0.0018 0.0014 0.002 0.0064 0.0024 0.002 0.0003 0.0014 0.0021 0.0027 0.001 0.0004 0.0003 0.0004 0.0009 0.0028 0.002 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.2485 7.0533 11.2729 9.9513 3.1477 13.9517 9.7373 15.1805 12.466 8.4864
Translational Efficiency 1.0352 0.7991 0.4766 0.793 1.5667 0.3997 0.751 0.4309 0.6123 0.9403

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
122 1047 1757 1067 991 1037 2544 1912 1113 2084 4301 2979

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 903.44 741.56 832.18 867.83 684.30 776.77 814.61 859.07 708.32 759.08 821.79 862.21
Standard Deviation 172.71 101.07 116.66 117.38 76.30 98.24 98.67 108.48 114.63 101.21 106.74 111.82
Intensity Change Log 2 -0.284865 -0.118533 -0.058016 0.182859 0.251481 0.328147 -0.064489 0.052682 0.121210

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001062 0.003056 0.006477 0.002561 0.001085 0.001452 0.005936 0.003278 0.001083 0.002258 0.006157 0.003021
Bud Neck 0.008114 0.020269 0.010391 0.008135 0.029371 0.034851 0.030508 0.015305 0.027041 0.027525 0.022290 0.012737
Bud Site 0.049584 0.027982 0.039397 0.041168 0.009883 0.018801 0.039627 0.032782 0.014235 0.023414 0.039533 0.035785
Cell Periphery 0.000587 0.000173 0.000085 0.000143 0.000285 0.000283 0.000105 0.000089 0.000318 0.000228 0.000097 0.000109
Cytoplasm 0.221663* 0.168394* 0.219358* 0.364910* 0.171699* 0.176330* 0.279644* 0.461623* 0.177176* 0.172343* 0.255016* 0.426983*
Cytoplasmic Foci 0.142896 0.452574* 0.317684* 0.142649 0.457414* 0.418117* 0.210056* 0.130529 0.422939* 0.435428* 0.254023* 0.134870
Eisosomes 0.000108 0.000304 0.000063 0.000115 0.000815 0.000583 0.000106 0.000083 0.000737 0.000443 0.000089 0.000095
Endoplasmic Reticulum 0.002338 0.001687 0.002090 0.001951 0.001924 0.001091 0.001516 0.000773 0.001969 0.001390 0.001750 0.001195
Endosome 0.069118 0.096719 0.054067 0.027361 0.065760 0.097784 0.048221 0.022143 0.066128 0.097249 0.050609 0.024012
Golgi 0.024860 0.068623 0.025254 0.008658 0.075356 0.060684 0.007258 0.003992 0.069821 0.064672 0.014609 0.005663
Lipid Particles 0.004210 0.017218 0.009373 0.004694 0.042593 0.021674 0.009136 0.002601 0.038386 0.019435 0.009233 0.003351
Mitochondria 0.009210 0.027642 0.004106 0.004630 0.050180 0.041163 0.007139 0.003758 0.045689 0.034370 0.005900 0.004070
Mitotic Spindle 0.027204 0.008178 0.162961* 0.050596 0.001012 0.005142 0.072337 0.042012 0.003883 0.006667 0.109358* 0.045086
None 0.000418 0.000782 0.001073 0.002155 0.001526 0.000800 0.001807 0.001148 0.001405 0.000791 0.001507 0.001509
Nuclear Periphery 0.000658 0.000245 0.000676 0.000828 0.000228 0.000363 0.001229 0.000633 0.000275 0.000303 0.001003 0.000703
Nuclear Periphery Foci 0.001781 0.000676 0.006287 0.002433 0.000221 0.000199 0.001278 0.000786 0.000392 0.000439 0.003324 0.001376
Nucleolus 0.000524 0.000922 0.002061 0.000529 0.001222 0.000743 0.002507 0.000680 0.001145 0.000833 0.002325 0.000626
Nucleus 0.041048 0.008802 0.021551 0.055700 0.015954 0.014897 0.060231 0.060860 0.018705 0.011835 0.044429 0.059012
Peroxisomes 0.017071 0.037587 0.044846 0.024529 0.035799 0.039024 0.090541 0.031102 0.033746 0.038302 0.071874 0.028748
Vacuole 0.372363* 0.051102 0.070270 0.252059* 0.032295 0.057876 0.129807 0.184379 0.069571 0.054473 0.105485 0.208620*
Vacuole Periphery 0.005183 0.007064 0.001931 0.004196 0.005378 0.008145 0.001010 0.001443 0.005356 0.007602 0.001386 0.002429

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.69 -5.55 -1.66 1.84 4.82 -1.33 -7.79 -2.62 -1.45 4.66 -2.96 -9.11 -3.05 0.75 6.59
Bud Neck -6.83 -3.09 -0.39 7.63 4.41 -1.45 -0.48 5.64 8.15 12.34 -0.20 1.64 6.23 10.26 12.41
Bud Site 1.49 0.52 1.57 0.36 3.49 -4.54 -10.78 -6.31 -1.38 4.34 -5.05 -10.55 -4.93 -0.18 5.76
Cell Periphery 2.15 2.56 2.47 3.50 -1.92 0.05 6.47 7.36 8.02 4.64 2.50 6.58 6.84 8.95 1.97
Cytoplasm 3.02 0.38 -4.53 -16.42 -10.71 -0.57 -13.48 -27.81 -27.00 -16.21 0.87 -11.22 -27.11 -32.01 -19.75
Cytoplasmic Foci -22.14 -12.21 1.54 43.44 27.47* 4.05 34.59 47.03 40.29 16.75 -1.77 24.32 42.98 59.54 30.53*
Eisosomes -7.31 2.38 0.83 9.71 -3.74 2.76 11.89 12.34 8.54 6.33 4.72 12.07 12.18 11.44 1.68
Endoplasmic Reticulum 0.25 -0.73 -0.05 -0.51 2.02 2.25 0.66 3.52 0.75 8.29 1.55 -0.15 2.46 0.58 7.11
Endosome -4.79 -0.05 3.72 18.37 14.00 -6.22 6.54 15.56 19.51 13.64 -8.06 5.69 15.90 27.00 19.54
Golgi -6.83 -0.71 1.91 18.15 10.46 2.85 18.03 18.90 16.99 6.11 1.04 15.78 18.44 25.28 12.50
Lipid Particles -6.31 -4.13 -0.08 7.92 9.10 6.40 12.28 14.74 10.52 15.37 6.87 11.76 14.42 13.26 17.35
Mitochondria -4.33 0.59 0.92 10.51 1.46 1.94 12.61 13.82 12.04 5.50 3.02 12.76 13.66 16.06 5.10
Mitotic Spindle 1.28 -10.70 -1.44 -6.82 15.72 -3.16 -19.62* -11.03 -8.64 8.59 -2.10 -28.86 -11.59 -10.82 17.59
None -1.31 -3.51 -3.43 -2.62 -1.89 0.84 -0.22 0.23 -2.49 1.19 0.81 -0.11 -0.21 -3.67 -0.31
Nuclear Periphery 2.51 -0.34 -3.22 -9.11 -5.19 -0.61 -11.65 -11.59 -4.01 5.07 -0.15 -11.64 -12.79 -8.02 2.14
Nuclear Periphery Foci 1.57 -6.34 -1.88 -6.13 6.07 0.69 -9.76 -7.96 -9.40 4.06 -0.36 -10.65 -10.19 -7.28 7.12
Nucleolus -1.28 -4.14 -0.81 0.96 4.04 0.94 -3.56 0.46 -1.57 6.52 0.65 -3.63 0.59 -0.25 7.52
Nucleus 4.41 2.35 -2.91 -18.86 -12.95 0.68 -20.24 -22.51 -22.18 -2.43 4.55 -15.58 -23.39 -29.89 -9.40
Peroxisomes -4.02 -4.85 0.02 8.43 10.04 -1.01 -10.24 5.88 6.51 15.61 -1.90 -9.70 6.64 10.07 18.25
Vacuole 12.87 11.66 2.18 -32.93 -28.94 -6.67 -31.27 -39.46 -31.96 -14.95 3.60 -10.48 -32.30 -45.85 -29.79
Vacuole Periphery -2.54 1.55 1.22 5.71 -0.29 -2.60 11.47 11.01 7.37 0.29 -3.51 10.53 8.79 10.16 0.02
Standard name
Human Ortholog
Description Protein of unknown function; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
Localization
Cell Percentages mitochondrion (11%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Apm2

Apm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available