Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0.07 0 0 0.07
Bud 0 0.05 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 – 0.05 0.06 0.06 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 – 0 0.09 0 0.06 0.09 0.08
Mitochondria 0.78 0.79 0.82 0.83 0.9 0.9 0.91 0.87 0.89 0.93 0.91 0.95 0.85 0.75 0.68 0.1 0.09 – 0.71 0.66 0.65 0.63 0.55 0.49
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.12 0 0.06 0 0.06 0.16 0.17 0.21 0.11 0.21 0.11 0.16 0 0 0 0.1 0.12 – 0 0 0 0 0 0
Peroxisomes 0.16 0.16 0.15 0.23 0.06 0 0 0 0 0 0 0 0.16 0.23 0.34 0.42 0.28 – 0.07 0 0.09 0.14 0.13 0.16
SpindlePole 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.19 – 0 0 0 0 0.06 0.07
Vac/Vac Membrane 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.1 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 0 1 0 1 1 2 2 3 1 1 – 5 6 7 6 12 29
Bud 7 6 4 5 4 3 0 6 3 0 3 3 6 15 13 2 3 – 3 1 1 3 4 10
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 1 0 1 0 3
Bud Site 4 0 1 2 0 0 1 0 0 0 2 0 1 1 0 4 1 – – – – – – –
Cell Periphery 7 2 1 0 0 2 1 0 1 0 1 0 6 1 6 5 2 – 0 0 0 0 0 0
Cytoplasm 2 1 2 1 1 8 7 7 10 6 6 8 7 6 9 4 3 – 1 0 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 2 3 0 0 0 1 0 0 0 3 0 1 3 – 0 0 1 0 0 0
Endosome 11 3 1 0 0 0 0 0 0 1 1 0 10 16 10 4 8 – 13 8 6 5 15 22
Golgi 0 4 0 0 0 0 1 1 0 0 1 0 3 6 8 1 18 – 7 12 3 14 33 31
Mitochondria 211 102 65 59 86 157 186 165 143 210 139 240 334 353 377 6 7 – 191 89 67 144 200 199
Nucleus 1 1 0 0 0 0 1 2 0 0 0 0 0 0 0 2 0 – 0 0 0 0 1 0
Nuclear Periphery 11 0 2 0 2 2 7 8 7 4 5 3 2 2 2 2 1 – 1 0 0 1 3 2
Nucleolus 33 5 5 3 6 27 34 39 18 47 17 40 11 15 14 6 9 – 0 1 1 1 6 2
Peroxisomes 44 20 12 16 6 1 2 0 0 0 2 1 63 110 188 26 22 – 19 5 9 31 45 66
SpindlePole 20 4 3 3 2 0 2 1 0 0 0 0 8 21 20 5 15 – 10 2 1 9 20 28
Vac/Vac Membrane 13 13 2 1 2 6 5 7 2 6 1 4 21 17 22 5 8 – 1 5 1 6 8 6
Unique Cell Count 269 129 79 71 96 174 204 190 161 225 153 252 395 469 552 62 78 270 136 104 231 362 410
Labelled Cell Count 364 162 99 90 111 209 248 236 185 275 179 300 474 568 672 74 101 270 136 104 231 362 410


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 12.9 10.0 10.4 22.0 26.4 27.1 28.3 25.4 28.1 24.4 26.0 16.6 17.4 18.4 15.6 20.1 – 11.0 11.2 12.3
Std Deviation (1e-4) 2.6 4.4 3.5 3.1 8.1 10.3 11.1 10.9 9.9 12.6 12.6 10.6 5.9 6.2 5.8 7.1 10.3 – 3.8 3.6 3.8
Intensity Change (Log2) – – – 0.06 1.14 1.4 1.44 1.5 1.35 1.49 1.29 1.38 0.73 0.8 0.88 0.65 1.01 – 0.13 0.17 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 4.5 –
Mitochondria 0.1 1.4 1.8 2.1 1.0 1.4 2.9 1.9 3.7 0.5 -1.4 -2.5 -8.6 -9.2 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 2.0 2.3 2.9 1.2 3.0 1.2 2.2 0 0 0 0 1.1 –
Peroxisomes 1.2 -1.9 0 0 0 0 0 0 0 0.2 1.6 3.4 3.6 2.0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.703 19.0537 16.8669 16.3009 15.421 18.6784 20.246 22.6057 21.0018 18.6216 19.7244 23.4096 27.2899 31.4625 30.8792 29.2855 25.4582 29.5094
Actin 0.0206 0.0021 0.0147 0.0021 0.007 0.0082 0.0195 0.0025 0.0007 0 0.0022 0.0002 0.0093 0.0018 0.0018 0.0019 0 0.0015
Bud 0.0002 0.0001 0.0003 0 0.0002 0.0001 0.0001 0 0 0 0 0 0 0 0.0001 0.0005 0 0.0001
Bud Neck 0.0003 0.0001 0.0007 0 0.0003 0.0001 0.0003 0 0 0 0 0 0 0 0 0.0001 0 0.0001
Bud Periphery 0.0004 0.0001 0.0003 0.0001 0.0005 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0 0.0003 0.001 0 0.0001
Bud Site 0.0011 0.0009 0.0032 0 0.0009 0.0001 0.0009 0 0.0001 0 0 0 0.0001 0 0.0002 0.0008 0 0
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0 0 0.0006 0.0004 0 0
Cytoplasm 0.0024 0 0 0 0.0001 0 0.0013 0 0 0 0 0 0.0007 0 0 0 0 0.0008
Cytoplasmic Foci 0.0146 0.0022 0.0029 0.0009 0.0111 0.0014 0.0133 0.0002 0.0042 0 0.0008 0 0.0058 0.0001 0.0002 0.0009 0 0.0004
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0003 0.0012 0.0004 0.0003 0.0001 0.0001 0.0001 0.0003 0.0003 0.0007 0.0004 0.0002 0 0.0002
Endoplasmic Reticulum 0.002 0 0.0001 0 0.0009 0.0001 0.0004 0 0 0 0 0 0.0007 0 0 0 0 0.0006
Endosome 0.0135 0.0032 0.0035 0.0046 0.0106 0.0019 0.01 0.0017 0.0004 0 0.0009 0.0004 0.0062 0.0001 0.0002 0.0009 0 0.0032
Golgi 0.0095 0.0097 0.0096 0.0358 0.0064 0.0083 0.0039 0.0016 0.0007 0.0001 0.0069 0.0003 0.0022 0.0011 0.0005 0.0007 0 0.0061
Lipid Particles 0.05 0.0143 0.0034 0.0079 0.0156 0.0088 0.027 0.007 0.0025 0.0001 0.0021 0.0006 0.007 0.0017 0.0018 0.0052 0.0001 0.0064
Mitochondria 0.8379 0.9471 0.9461 0.9411 0.9032 0.9535 0.8838 0.9776 0.9773 0.9992 0.9801 0.9907 0.9532 0.9914 0.9587 0.9606 0.9996 0.9616
None 0.0032 0 0 0 0.0003 0 0.0022 0 0 0 0 0 0.0015 0 0 0 0 0.0002
Nuclear Periphery 0.004 0 0.0001 0 0.0029 0.0001 0.001 0 0 0 0 0.0001 0.0032 0 0 0.0001 0 0.008
Nucleolus 0.007 0.0002 0.0001 0 0.0004 0.0002 0.0007 0.0001 0.0003 0 0 0 0.0001 0 0.0098 0.0006 0 0.0002
Nucleus 0.0046 0.0001 0 0 0.0007 0 0.0003 0 0.0001 0 0 0 0.0002 0 0.0011 0.0002 0 0.004
Peroxisomes 0.0134 0.0093 0.0049 0.0028 0.0356 0.0036 0.0116 0.0018 0.0046 0.0002 0.0064 0.0004 0.0031 0.0016 0.003 0.0086 0 0.0005
Punctate Nuclear 0.0058 0 0 0 0.001 0 0.0064 0 0.0001 0 0 0 0.005 0 0 0 0 0.0006
Vacuole 0.0014 0.0019 0.0012 0.0005 0.0009 0.001 0.005 0.0014 0.0028 0 0 0.0012 0.0002 0.0001 0.0069 0.0048 0 0.0006
Vacuole Periphery 0.0073 0.0084 0.0085 0.0038 0.0013 0.0112 0.0116 0.0057 0.0058 0.0002 0.0002 0.0058 0.001 0.0012 0.0143 0.0126 0.0002 0.0047

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.8621 13.4057 20.4188 56.621 26.0949 48.119 35.313 63.3163 72.6359 33.4435
Translational Efficiency 1.4401 1.7942 1.7326 1.3178 1.1218 1.9961 1.8416 1.8752 2.0659 1.8434

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1203 1456 223 1305 837 1439 199 154 2040 2895 422 1459

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 996.18 1083.13 3442.02 1296.63 1575.57 1085.52 1960.56 1402.54 1233.90 1084.32 2743.42 1307.81
Standard Deviation 250.54 301.58 985.12 396.71 611.59 262.70 489.48 495.02 521.25 282.93 1082.92 409.50
Intensity Change Log 2 — 0.120728 1.788777 0.380289 — -0.537488 0.315392 -0.167832 — -0.245953 1.070898 0.069765

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.008370 0.009418 0.007259 0.021916 0.005321 0.008619 0.005537 0.023498 0.007119 0.009021 0.006447 0.022083
Bud Neck 0.004110 0.003025 0.010089 0.003378 0.002746 0.001749 0.010341 0.015878 0.003550 0.002391 0.010208 0.004697
Bud Site 0.012414 0.006588 0.003994 0.015682 0.002790 0.003508 0.005454 0.022498 0.008465 0.005057 0.004682 0.016401
Cell Periphery 0.000470 0.000288 0.000140 0.000547 0.000113 0.000138 0.000209 0.000548 0.000323 0.000213 0.000172 0.000547
Cytoplasm 0.002874 0.001156 0.000982 0.002236 0.000516 0.001130 0.000561 0.002110 0.001907 0.001143 0.000783 0.002223
Cytoplasmic Foci 0.033310 0.026099 0.108608 0.019680 0.059546 0.031528 0.064847 0.029393 0.044074 0.028798 0.087972 0.020706
Eisosomes 0.000508 0.000592 0.002918 0.000935 0.001675 0.000266 0.000549 0.000439 0.000987 0.000430 0.001801 0.000882
Endoplasmic Reticulum 0.000573 0.000192 0.000136 0.000756 0.000226 0.000203 0.000307 0.002068 0.000431 0.000197 0.000217 0.000895
Endosome 0.016334 0.012466 0.001502 0.016453 0.003835 0.017925 0.014203 0.032006 0.011206 0.015179 0.007491 0.018094
Golgi 0.025546 0.028914 0.019908 0.056360 0.022635 0.047895 0.061037 0.096280 0.024351 0.038349 0.039303 0.060574
Lipid Particles 0.014666 0.009488 0.018044 0.004304 0.017227 0.007872 0.017005 0.004100 0.015717 0.008685 0.017554 0.004282
Mitochondria 0.805724* 0.838927* 0.721963* 0.802319* 0.830027* 0.823310* 0.726673* 0.663494* 0.815696* 0.831164* 0.724184* 0.787666*
Mitotic Spindle 0.002450 0.002157 0.022508 0.007825 0.003032 0.003444 0.019060 0.011049 0.002689 0.002797 0.020882 0.008165
None 0.001463 0.000623 0.007347 0.000794 0.002828 0.000474 0.002253 0.001217 0.002023 0.000549 0.004945 0.000839
Nuclear Periphery 0.000395 0.000200 0.006549 0.000652 0.000600 0.000123 0.005622 0.003032 0.000479 0.000162 0.006112 0.000903
Nuclear Periphery Foci 0.000773 0.000676 0.001703 0.000359 0.000691 0.000220 0.002181 0.001050 0.000739 0.000449 0.001928 0.000432
Nucleolus 0.003331 0.000700 0.007110 0.000146 0.002779 0.000223 0.002005 0.000516 0.003104 0.000463 0.004703 0.000185
Nucleus 0.000668 0.000169 0.000575 0.000113 0.000219 0.000075 0.000340 0.002596 0.000484 0.000123 0.000464 0.000375
Peroxisomes 0.048188 0.052303 0.023801 0.025086 0.033247 0.045788 0.020834 0.025890 0.042058 0.049065 0.022402 0.025171
Vacuole 0.009295 0.003986 0.015656 0.008637 0.005356 0.003001 0.016691 0.030318 0.007679 0.003496 0.016144 0.010926
Vacuole Periphery 0.008537 0.002034 0.019210 0.011822 0.004590 0.002510 0.024291 0.032020 0.006917 0.002271 0.021606 0.013954

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.55 0.64 -5.11 -4.80 -5.71 -2.46 -0.17 -2.38 -1.93 -2.34 -1.56 0.61 -6.38 -5.57 -6.75
Bud Neck 1.91 -3.89 1.03 -0.62 4.25 2.73 -3.34 -2.07 -2.23 -0.81 3.28 -4.97 -1.38 -2.77 3.59
Bud Site 2.85 4.60 -1.26 -3.73 -5.12 -0.66 -1.57 -2.71 -2.61 -2.31 2.71 2.83 -3.45 -5.19 -5.26
Cell Periphery 2.58 4.93 -0.87 -3.06 -4.89 -1.17 -2.43 -3.19 -3.03 -2.43 2.70 3.46 -2.92 -4.64 -5.07
Cytoplasm 2.43 1.51 0.74 -3.01 -1.17 -3.29 -0.42 -1.82 -1.10 -1.76 1.80 1.64 -0.71 -3.29 -2.59
Cytoplasmic Foci 2.26 -6.45 4.49 2.36 7.68 5.97 -0.57 3.61 0.29 3.23 5.65 -5.79 8.24 3.60 9.01
Eisosomes -1.06 -5.82 -1.44 -1.15 3.93 9.40 6.72 6.28 -1.31 0.72 7.30 -3.41 0.39 -1.72 2.64
Endoplasmic Reticulum 5.56 6.27 -0.90 -2.93 -3.19 0.67 -1.60 -2.44 -2.47 -2.33 5.38 4.51 -2.39 -3.67 -3.54
Endosome 2.03 9.85 -0.06 -2.27 -11.39 -10.37 -3.48 -4.43 -2.19 -2.55 -3.16 2.17 -4.26 -1.84 -5.42
Golgi -1.16 1.70 -7.39 -6.45 -8.02 -6.82 -3.94 -4.83 -3.15 -1.98 -5.95 -2.98 -9.17 -5.42 -3.52
Lipid Particles 2.53 -0.84 5.84 3.18 3.58 4.23 0.05 5.84 2.81 2.95 4.77 -0.60 7.79 3.83 4.54
Mitochondria -2.89 3.82 0.33 3.38 -3.68 0.55 4.13 5.24 5.10 1.64 -1.86 5.53 2.76 4.57 -3.69
Mitotic Spindle 0.45 -3.44 -4.53 -4.85 2.48 -0.49 -3.10 -2.01 -1.89 1.24 -0.19 -4.63 -4.94 -4.75 3.13
None 7.76 -6.10 5.92 -3.01 6.83 11.97 1.98 6.39 -4.56 3.84 14.21 -5.39 10.67 -5.80 7.71
Nuclear Periphery 1.20 -3.20 -1.32 -2.54 3.06 5.78 -3.09 -1.20 -1.43 1.00 3.26 -4.42 -1.61 -2.89 4.02
Nuclear Periphery Foci 0.23 -2.41 3.12 0.74 3.37 3.53 -2.48 -0.89 -2.12 1.61 1.29 -3.44 2.74 0.07 4.28
Nucleolus 3.10 -1.82 3.86 3.01 3.61 3.85 0.81 3.23 -1.21 2.18 4.66 -1.35 5.23 2.69 4.22
Nucleus 3.93 -0.29 3.30 -0.02 1.63 3.65 -0.81 -1.06 -1.12 -1.00 4.77 -0.41 0.18 -1.21 0.41
Peroxisomes -0.76 4.31 5.01 5.86 -0.26 -2.60 2.14 0.93 2.53 -0.58 -1.91 4.81 4.64 6.67 -0.67
Vacuole 4.20 -2.58 0.39 -3.79 2.78 2.76 -3.86 -3.50 -3.84 -1.79 5.18 -4.35 -2.40 -5.88 2.51
Vacuole Periphery 6.11 -2.94 -2.05 -7.91 2.04 3.70 -3.84 -4.49 -4.84 -0.97 6.92 -4.75 -4.92 -9.08 2.36
Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Aim17

Aim17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available