Standard name
Human Ortholog
Description Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.13 0.07 0.15 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 1.0 0.99 0.99 0.95 – 0.88 0.92 0.81 0.85 0.97 0.99 1.0 0.91 0.95 0.96 0.98 0.97 0.92 0.99 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 – 0.13 0.07 0.3 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 – 1 1 1 0 0 0 0 2 1 0 0 0 0 0 0 0
Bud 0 0 1 0 1 0 7 – 3 6 7 10 1 0 0 0 1 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 1 0 – 1 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 11 7 9 8 9 10 – 0 1 3 2 0 6 6 36 20 24 0 0 0 0 0 0
Cytoplasm 189 187 133 213 311 348 488 – 184 321 300 393 60 254 301 248 267 152 185 181 135 65 188 188
Endoplasmic Reticulum 0 1 0 0 1 0 1 – 1 5 0 14 1 3 0 13 11 14 0 0 1 0 0 0
Endosome 0 0 1 0 0 1 3 – 3 1 2 0 0 0 0 4 2 1 0 0 1 0 0 0
Golgi 0 0 0 0 2 0 0 – 0 0 0 0 0 0 0 3 4 2 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 10 35 – 28 23 110 76 0 1 0 4 2 1 0 1 2 0 1 1
Nucleus 0 1 2 2 2 1 1 – 3 3 2 4 1 1 0 4 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 3 0 0 3 0 0 0 0 0 0 0 0 0 0 2 2
Vac/Vac Membrane 0 0 0 0 0 1 4 – 1 3 3 3 0 1 0 9 7 4 1 1 3 0 0 0
Unique Cell Count 189 188 135 213 314 353 516 208 350 369 463 62 257 301 273 281 158 189 186 148 66 202 202
Labelled Cell Count 190 200 144 224 325 371 554 228 369 429 506 63 266 307 323 317 198 189 186 148 66 202 202


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.9 6.4 7.3 7.4 5.8 5.5 – 4.7 4.7 4.2 4.5 6.8 7.8 6.9 9.7 10.1 10.3 5.7 6.6 6.6
Std Deviation (1e-4) 0.8 1.1 0.9 1.1 1.1 1.1 1.0 – 1.1 0.8 0.8 0.7 0.8 1.1 1.0 2.1 2.2 2.4 0.6 1.2 1.2
Intensity Change (Log2) – – – 0.19 0.21 -0.15 -0.22 – -0.44 -0.45 -0.62 -0.53 0.08 0.28 0.1 0.59 0.65 0.67 -0.17 0.03 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 0 0 0 – 0 0 0 0 0 0 0 2.5 0.7 2.8
Cytoplasm 1.8 0.5 0.1 -1.9 – -3.4 -2.7 -4.9 -4.3 -0.8 0.3 2.1 -2.9 -1.7 -1.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3.5
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0008 5.0894 4.3204 3.8214 4.0729 3.9463 5.0595 6.7172 6.0538 5.764 5.206 5.8098 3.2159 5.3269 4.4178 4.3733 4.0145 4.297
Actin 0.0365 0.0004 0.0139 0.0282 0.0434 0.0024 0.0266 0.0002 0.0085 0.0162 0.0035 0.0018 0.0642 0.0003 0.0237 0.0024 0.0005 0.0026
Bud 0.0007 0.0002 0.0004 0.0061 0.0004 0.0002 0.001 0.0002 0.001 0.0032 0.0004 0.0005 0.0035 0.0022 0.004 0.0002 0.0001 0.0009
Bud Neck 0.0024 0.0001 0.0007 0.0084 0.0017 0.0007 0.0021 0.0001 0.0009 0.0021 0.0015 0.001 0.0041 0.0001 0.0004 0.0003 0.0004 0.0012
Bud Periphery 0.0006 0.0001 0.0004 0.0087 0.0003 0.0002 0.0012 0.0001 0.0015 0.0032 0.0003 0.0005 0.0046 0.0003 0.0015 0 0.0002 0.0009
Bud Site 0.0076 0.0014 0.0037 0.0096 0.0041 0.0003 0.0042 0.0004 0.0037 0.011 0.0005 0.0005 0.0114 0.0008 0.004 0.0002 0.0003 0.0078
Cell Periphery 0.0006 0.0004 0.0003 0.0009 0.0007 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0.0006 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.7802 0.9764 0.9157 0.8081 0.9221 0.944 0.7906 0.9626 0.9045 0.8769 0.8061 0.943 0.7208 0.9816 0.9123 0.9256 0.8493 0.9441
Cytoplasmic Foci 0.0342 0.0029 0.0145 0.0134 0.0061 0.005 0.0222 0.0073 0.0125 0.0216 0.0481 0.0088 0.034 0.0026 0.0078 0.0261 0.0044 0.0066
Eisosomes 0.0004 0 0.0001 0.0007 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0001 0 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0066 0.0031 0.0032 0.0037 0.003 0.002 0.0113 0.0014 0.0043 0.0037 0.0044 0.0037 0.0183 0.0018 0.0025 0.0018 0.0043 0.0058
Endosome 0.0305 0.0019 0.0177 0.0235 0.0064 0.0093 0.0411 0.006 0.0193 0.021 0.0483 0.0093 0.0316 0.0011 0.0086 0.0068 0.0323 0.0102
Golgi 0.0117 0.0002 0.0073 0.0232 0.0029 0.0056 0.0134 0.0006 0.0088 0.0059 0.0147 0.0023 0.0117 0.0001 0.0029 0.0009 0.0017 0.0022
Lipid Particles 0.009 0.0001 0.0048 0.0115 0.0007 0.0041 0.0106 0.0005 0.0043 0.0006 0.0143 0.0022 0.0084 0 0.0039 0.0059 0.0052 0.0018
Mitochondria 0.0034 0.0001 0.0056 0.0045 0.0002 0.0183 0.0091 0.0003 0.0051 0.0043 0.0282 0.0144 0.0174 0.0003 0.0094 0.0001 0.0039 0.0072
None 0.0385 0.0104 0.0053 0.0062 0.0039 0.0041 0.021 0.0172 0.0131 0.0179 0.0126 0.0016 0.0204 0.0065 0.0061 0.0037 0.0607 0.0018
Nuclear Periphery 0.0025 0.0004 0.0009 0.0021 0.0005 0.0005 0.0098 0.0003 0.0013 0.0013 0.0008 0.0025 0.0068 0.0003 0.0009 0.0008 0.0173 0.0005
Nucleolus 0.0007 0 0.0001 0.0011 0 0.0001 0.0009 0 0.0002 0.0002 0.0001 0.0004 0.0007 0.0001 0.0001 0.0001 0.0009 0.0003
Nucleus 0.0012 0.0006 0.0007 0.0018 0.0007 0.0005 0.012 0.0007 0.0014 0.0028 0.0011 0.0019 0.004 0.0009 0.0008 0.001 0.0048 0.0009
Peroxisomes 0.0092 0 0.0019 0.0059 0.0002 0.0014 0.0121 0.001 0.003 0.0029 0.0108 0.0017 0.0122 0 0.0085 0.008 0.0002 0.0033
Punctate Nuclear 0.0203 0.0001 0.0004 0.0047 0.0002 0.0002 0.0026 0.0001 0.0028 0.0012 0.0005 0.001 0.0186 0.0001 0.0007 0.013 0.0023 0.0005
Vacuole 0.0025 0.001 0.002 0.022 0.0019 0.0007 0.0062 0.001 0.0028 0.0033 0.0028 0.002 0.0047 0.0007 0.0013 0.0025 0.0059 0.0011
Vacuole Periphery 0.0007 0.0001 0.0005 0.0057 0.0005 0.0005 0.0016 0.0002 0.0007 0.0007 0.0009 0.0009 0.0016 0.0001 0.0003 0.0003 0.0053 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.8014 14.7064 21.4358 22.4866 20.5679 17.6165 23.2337 26.1652 28.9999 28.1755
Translational Efficiency 1.9368 2.599 1.4095 1.3949 1.8929 2.2592 1.512 1.6328 1.3933 1.3214

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2006 958 1704 1328 1975 1880 2405 866 3981 2838 4109 2194

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 623.90 798.54 1065.42 1144.85 819.07 850.91 1101.92 1227.10 720.73 833.23 1086.78 1177.32
Standard Deviation 77.73 128.35 151.73 180.12 101.36 111.01 147.74 164.65 132.91 119.74 150.49 178.76
Intensity Change Log 2 — 0.356050 0.772036 0.875772 — 0.055020 0.427961 0.583194 — 0.192944 0.586884 0.717032

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000170 0.000361 0.000144 0.000928 0.000226 0.000448 0.000176 0.000465 0.000198 0.000419 0.000163 0.000745
Bud Neck 0.019073 0.011608 0.002675 0.003591 0.005397 0.010838 0.001409 0.002554 0.012288 0.011098 0.001934 0.003182
Bud Site 0.013791 0.032682 0.006921 0.031249 0.006272 0.026867 0.013641 0.029968 0.010061 0.028829 0.010855 0.030743
Cell Periphery 0.000271 0.000108 0.000181 0.000126 0.000072 0.000127 0.000234 0.000113 0.000172 0.000121 0.000212 0.000120
Cytoplasm 0.561394* 0.548259* 0.661643* 0.695059* 0.618440* 0.563484* 0.866864* 0.734999* 0.589695* 0.558344* 0.781759* 0.710824*
Cytoplasmic Foci 0.213845* 0.214912* 0.018291 0.051907 0.167977 0.193749* 0.019199 0.065708 0.191090* 0.200893* 0.018823 0.057355
Eisosomes 0.000276 0.000055 0.000036 0.000011 0.000054 0.000069 0.000005 0.000010 0.000166 0.000065 0.000018 0.000011
Endoplasmic Reticulum 0.002104 0.000540 0.001327 0.000412 0.001032 0.000939 0.000159 0.000137 0.001572 0.000804 0.000644 0.000304
Endosome 0.008908 0.036815 0.002416 0.011587 0.015718 0.029519 0.001376 0.008645 0.012286 0.031982 0.001807 0.010425
Golgi 0.005517 0.006124 0.000095 0.005730 0.002486 0.008337 0.000655 0.006386 0.004013 0.007590 0.000423 0.005989
Lipid Particles 0.007625 0.002377 0.000871 0.001242 0.002222 0.002608 0.000561 0.001408 0.004944 0.002530 0.000690 0.001307
Mitochondria 0.006232 0.001109 0.000288 0.004077 0.001216 0.003116 0.000151 0.000923 0.003744 0.002439 0.000208 0.002832
Mitotic Spindle 0.000125 0.001172 0.002615 0.019163 0.002506 0.007238 0.007689 0.012548 0.001306 0.005191 0.005585 0.016552
None 0.006007 0.003615 0.003607 0.000463 0.001335 0.001048 0.001903 0.000557 0.003689 0.001914 0.002610 0.000500
Nuclear Periphery 0.000202 0.000076 0.000717 0.000299 0.000143 0.000081 0.000078 0.000102 0.000173 0.000079 0.000343 0.000221
Nuclear Periphery Foci 0.000252 0.000782 0.002059 0.001824 0.000675 0.000385 0.001100 0.001798 0.000462 0.000519 0.001498 0.001814
Nucleolus 0.000645 0.000903 0.000259 0.000222 0.000491 0.000315 0.000121 0.000699 0.000569 0.000513 0.000178 0.000410
Nucleus 0.099284 0.013499 0.184081* 0.008481 0.039646 0.013510 0.029908 0.008171 0.069698 0.013506 0.093844 0.008359
Peroxisomes 0.002352 0.004404 0.000215 0.004468 0.001564 0.003826 0.000383 0.001955 0.001961 0.004021 0.000313 0.003476
Vacuole 0.050068 0.117986 0.111348 0.155242 0.131109 0.130597 0.054017 0.121113 0.090273 0.126340 0.077792 0.141771
Vacuole Periphery 0.001860 0.002613 0.000211 0.003920 0.001420 0.002899 0.000370 0.001739 0.001642 0.002802 0.000305 0.003059

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.32 -0.86 -3.98 -2.24 -3.52 -1.60 0.65 -1.33 0.52 -3.23 -2.55 0.29 -3.86 -1.43 -4.60
Bud Neck 4.64 13.70 13.06 6.76 -1.64 -7.82 11.83 6.81 12.47 -3.48 1.53 16.45 14.21 13.39 -4.32
Bud Site -7.60 4.71 -5.12 2.75 -9.09 -12.89 -3.48 -7.45 0.91 -5.80 -13.31 1.89 -9.29 2.20 -10.45
Cell Periphery 11.72 4.29 10.18 0.43 1.34 -4.08 -3.09 -3.39 1.72 1.77 4.64 1.57 7.18 1.65 2.17
Cytoplasm 1.21 -6.08 -11.11 -9.99 -5.39 6.30 -28.37 -6.58 -11.12 12.20 4.73 -25.67 -12.05 -14.72 7.39
Cytoplasmic Foci 0.16 46.43 32.57 24.00 -14.57 -4.61 37.34 18.69 22.16 -12.59 -2.03 58.74 36.82 34.15 -18.66
Eisosomes 15.64 17.19 19.21 13.79 5.89 -2.58 19.94 17.25 11.04 -3.79 12.50 20.14 21.34 14.57 4.06
Endoplasmic Reticulum 3.87 1.46 4.62 3.42 5.84 0.33 4.84 5.03 4.38 1.31 3.03 3.71 6.17 4.69 5.54
Endosome -12.63 12.03 0.94 12.37 -5.98 -9.07 19.63 8.04 14.10 -4.86 -15.93 22.87 6.33 18.54 -7.98
Golgi -0.44 14.33 2.84 2.45 -3.42 -8.68 7.92 -0.60 3.36 -2.10 -6.15 15.74 1.22 4.92 -3.78
Lipid Particles 6.58 12.42 10.23 0.68 -5.10 -0.81 13.29 0.30 0.89 -6.02 5.19 14.88 9.48 1.29 -8.29
Mitochondria 8.84 11.19 4.36 -1.09 -2.13 -2.49 2.69 1.57 3.81 -1.55 2.40 10.58 3.88 1.52 -2.44
Mitotic Spindle -2.17 -4.79 -7.30 -6.55 -5.57 -2.89 -3.46 -4.48 -2.43 -2.24 -3.83 -5.27 -8.34 -5.62 -5.32
None 2.03 3.20 6.99 3.20 7.77 0.95 -2.28 4.75 1.93 7.38 3.34 3.08 7.82 3.76 10.83
Nuclear Periphery 5.91 -12.39 -1.12 -3.71 9.43 4.59 -2.38 0.53 -2.74 2.23 7.27 -11.92 -1.17 -4.22 7.60
Nuclear Periphery Foci -4.93 -12.71 -9.48 -6.02 1.96 4.35 -4.86 -7.74 -9.31 -4.98 -1.22 -13.29 -11.91 -11.36 -2.39
Nucleolus -0.94 3.94 4.02 2.70 0.36 2.32 4.58 0.30 -0.53 -1.29 0.58 6.00 2.52 1.70 -1.37
Nucleus 29.51 -16.10 32.27 2.20 38.54 20.40 0.99 22.89 4.30 23.63 34.41 -10.47 37.14 4.58 38.68
Peroxisomes -5.23 13.63 -1.68 1.56 -5.49 -7.33 7.90 -0.22 6.30 -5.69 -8.51 15.10 -1.89 3.27 -6.70
Vacuole -14.25 -20.25 -22.00 -9.27 -4.03 0.19 7.95 -4.75 -4.75 -9.42 -10.45 -9.41 -18.17 -10.04 -11.02
Vacuole Periphery -2.70 11.20 -0.27 1.06 -3.80 -6.68 7.33 1.36 5.23 -2.01 -6.68 12.55 0.18 3.47 -4.23
Standard name
Human Ortholog
Description Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rim101

Rim101


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rim101-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available