Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | – | – | 0 | 0 | 0 | 3 | – | 3 | 4 | – | 0 | 0 | 0 | 0 | 0 | – | 6 | 0 | 3 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | – | 0 | 0 | 0 | 2 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | – | 0 | 0 | 0 | 1 | – | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | – | 1 | 1 | 1 | 0 | – | 0 | 0 | – | 0 | 6 | 10 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 44 | 19 | – | – | 28 | 46 | 35 | 48 | – | 18 | 12 | – | 55 | 122 | 126 | 21 | 16 | – | 60 | 30 | 28 | 58 | 61 | 91 |
Endoplasmic Reticulum | 0 | 0 | – | – | 0 | 0 | 0 | 1 | – | 1 | 0 | – | 1 | 3 | 0 | 0 | 2 | – | 3 | 0 | 0 | 3 | 1 | 3 |
Endosome | 11 | 0 | – | – | 0 | 0 | 0 | 3 | – | 0 | 0 | – | 1 | 0 | 0 | 2 | 1 | – | 16 | 2 | 5 | 0 | 0 | 1 |
Golgi | 0 | 1 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 11 | 0 | – | – | 0 | 25 | 28 | 38 | – | 38 | 36 | – | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 1 | 1 | 2 |
Nucleus | 5 | 0 | – | – | 0 | 0 | 0 | 3 | – | 2 | 1 | – | 0 | 1 | 0 | 0 | 0 | – | 17 | 7 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 21 | 2 | – | – | 0 | 1 | 2 | 2 | – | 4 | 4 | – | 0 | 0 | 1 | 0 | 0 | – | 10 | 1 | 3 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | – | 0 | 0 | 1 | 2 | – | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | – | 12 | 1 | 6 | 1 | 0 | 0 |
Vac/Vac Membrane | 36 | 6 | – | – | 0 | 4 | 0 | 6 | – | 8 | 5 | – | 1 | 2 | 0 | 4 | 2 | – | 16 | 13 | 2 | 1 | 1 | 2 |
Unique Cell Count | 105 | 27 | 29 | 62 | 51 | 88 | 56 | 49 | 57 | 126 | 127 | 25 | 18 | 157 | 61 | 61 | 69 | 71 | 106 | |||||
Labelled Cell Count | 130 | 29 | 29 | 77 | 67 | 109 | 75 | 64 | 58 | 135 | 137 | 28 | 21 | 157 | 61 | 61 | 69 | 71 | 106 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 14.8 | – | – | 9.1 | 5.8 | 5.4 | 5.9 | – | 5.2 | 5.7 | – | 8.8 | 9.5 | 9.4 | 22.3 | 9.8 | – | 9.3 | 9.2 | 10.0 |
Std Deviation (1e-4) | 1.7 | 5.3 | – | – | 2.0 | 1.2 | 1.1 | 1.6 | – | 1.4 | 1.5 | – | 1.7 | 1.6 | 1.9 | 35.9 | 1.7 | – | 2.1 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2504.8086 | 2426.3414 | 2747.8678 | 2364.5553 | 2718.5111 | 2232.4192 | 2126.8964 | 1958.7816 | 2178.3014 | 1952.276 |
Translational Efficiency | 1.6633 | 1.7097 | 1.5986 | 1.4843 | 1.7106 | 1.9808 | 1.7353 | 2.1429 | 1.7211 | 2.1266 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1646 | 766 | 2111 | 98 | 492 | 1712 | 203 | 1151 | 2138 | 2478 | 2314 | 1249 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 935.12 | 1019.63 | 1136.15 | 1254.74 | 959.86 | 982.55 | 1262.93 | 1212.94 | 940.81 | 994.01 | 1147.27 | 1216.22 |
Standard Deviation | 152.73 | 199.38 | 177.22 | 207.23 | 138.48 | 147.13 | 240.58 | 204.30 | 149.94 | 165.95 | 187.12 | 204.84 |
Intensity Change Log 2 | — | 0.124822 | 0.280930 | 0.424165 | — | 0.033707 | 0.395879 | 0.337612 | — | 0.079389 | 0.340299 | 0.380973 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000028 | 0.000053 | 0.000055 | 0.000295 | 0.000040 | 0.000031 | 0.000213 | 0.000048 | 0.000031 | 0.000038 | 0.000069 | 0.000067 |
Bud Neck | 0.000791 | 0.001611 | 0.003612 | 0.005235 | 0.000943 | 0.000767 | 0.021469 | 0.001971 | 0.000826 | 0.001028 | 0.005179 | 0.002227 |
Bud Site | 0.001660 | 0.007328 | 0.003582 | 0.010159 | 0.003214 | 0.002461 | 0.005141 | 0.004335 | 0.002017 | 0.003965 | 0.003719 | 0.004792 |
Cell Periphery | 0.000457 | 0.000616 | 0.000415 | 0.000197 | 0.000252 | 0.000412 | 0.000581 | 0.000484 | 0.000409 | 0.000475 | 0.000430 | 0.000462 |
Cytoplasm | 0.000869 | 0.005653 | 0.000420 | 0.001845 | 0.003210 | 0.000636 | 0.017398 | 0.001266 | 0.001408 | 0.002187 | 0.001909 | 0.001312 |
Cytoplasmic Foci | 0.001710 | 0.005454 | 0.004641 | 0.012607 | 0.003667 | 0.002327 | 0.007137 | 0.003193 | 0.002160 | 0.003293 | 0.004860 | 0.003932 |
Eisosomes | 0.000005 | 0.000008 | 0.000052 | 0.000020 | 0.000006 | 0.000009 | 0.000190 | 0.000015 | 0.000005 | 0.000008 | 0.000064 | 0.000015 |
Endoplasmic Reticulum | 0.001243 | 0.000680 | 0.000693 | 0.000468 | 0.001090 | 0.000801 | 0.001890 | 0.000724 | 0.001208 | 0.000763 | 0.000798 | 0.000704 |
Endosome | 0.006922 | 0.019713 | 0.010717 | 0.039379 | 0.014419 | 0.013962 | 0.030717 | 0.010570 | 0.008648 | 0.015740 | 0.012471 | 0.012830 |
Golgi | 0.001544 | 0.005572 | 0.003453 | 0.019269 | 0.003701 | 0.003771 | 0.002583 | 0.012032 | 0.002040 | 0.004327 | 0.003376 | 0.012600 |
Lipid Particles | 0.000295 | 0.000436 | 0.000939 | 0.000645 | 0.002269 | 0.000580 | 0.006393 | 0.000573 | 0.000749 | 0.000535 | 0.001418 | 0.000579 |
Mitochondria | 0.009106 | 0.007918 | 0.006119 | 0.008956 | 0.013534 | 0.011043 | 0.012866 | 0.012049 | 0.010125 | 0.010077 | 0.006711 | 0.011806 |
Mitotic Spindle | 0.000097 | 0.000272 | 0.000816 | 0.002226 | 0.000333 | 0.000926 | 0.004907 | 0.001816 | 0.000151 | 0.000724 | 0.001175 | 0.001849 |
None | 0.000525 | 0.000467 | 0.000395 | 0.000264 | 0.000145 | 0.000301 | 0.001359 | 0.000256 | 0.000437 | 0.000352 | 0.000479 | 0.000256 |
Nuclear Periphery | 0.000332 | 0.000304 | 0.001828 | 0.002151 | 0.000570 | 0.001762 | 0.006305 | 0.002152 | 0.000387 | 0.001311 | 0.002221 | 0.002151 |
Nuclear Periphery Foci | 0.000169 | 0.000520 | 0.001337 | 0.001729 | 0.000389 | 0.001934 | 0.004608 | 0.001819 | 0.000220 | 0.001497 | 0.001624 | 0.001812 |
Nucleolus | 0.000602 | 0.001027 | 0.013073 | 0.004340 | 0.000433 | 0.002369 | 0.049003 | 0.002439 | 0.000563 | 0.001955 | 0.016225 | 0.002588 |
Nucleus | 0.000628 | 0.000972 | 0.008132 | 0.002613 | 0.000970 | 0.001832 | 0.067829 | 0.002567 | 0.000706 | 0.001567 | 0.013369 | 0.002570 |
Peroxisomes | 0.000245 | 0.000758 | 0.000764 | 0.006454 | 0.000800 | 0.000257 | 0.004316 | 0.000226 | 0.000373 | 0.000412 | 0.001076 | 0.000715 |
Vacuole | 0.178438 | 0.214586* | 0.347168* | 0.465778* | 0.228106* | 0.224079* | 0.389310* | 0.408637* | 0.189868 | 0.221145* | 0.350865* | 0.413121* |
Vacuole Periphery | 0.794335* | 0.726052* | 0.591789* | 0.415368* | 0.721909* | 0.729741* | 0.365783* | 0.532829* | 0.777669* | 0.728601* | 0.571963* | 0.523613* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.42 | -2.54 | -1.48 | -1.33 | -1.31 | 0.77 | -2.87 | -0.64 | -1.48 | 2.74 | -0.82 | -3.53 | -2.11 | -1.64 | 0.22 |
Bud Neck | -1.55 | -5.86 | -2.81 | -2.27 | -0.97 | 0.46 | -4.22 | -2.03 | -3.03 | 4.00 | -0.65 | -7.26 | -3.04 | -3.01 | 4.59 |
Bud Site | -4.33 | -3.66 | -1.98 | -0.61 | -1.51 | 0.63 | -1.26 | -0.86 | -2.13 | 0.56 | -3.33 | -3.20 | -3.19 | -0.91 | -1.16 |
Cell Periphery | -0.94 | 0.70 | 4.43 | 2.50 | 4.06 | -1.85 | -2.07 | -2.31 | -0.59 | 0.55 | -0.80 | -0.39 | -0.55 | 0.13 | -0.33 |
Cytoplasm | -2.69 | 1.69 | -1.27 | -0.55 | -1.34 | 1.97 | -2.13 | 1.22 | -1.82 | 2.45 | -1.14 | -0.70 | -0.97 | -0.00 | -0.32 |
Cytoplasmic Foci | -3.39 | -3.27 | -2.56 | -1.63 | -1.85 | 1.36 | -1.86 | 0.48 | -1.09 | 2.32 | -1.87 | -3.27 | -2.13 | -0.75 | 0.96 |
Eisosomes | -2.00 | -11.23 | -2.89 | -2.26 | 4.94 | -1.55 | -4.01 | -4.35 | -3.21 | 3.82 | -2.74 | -10.57 | -5.91 | -4.01 | 8.65 |
Endoplasmic Reticulum | 3.59 | 4.50 | 5.46 | 1.43 | 2.03 | 1.68 | -2.14 | 1.64 | 0.43 | 3.08 | 4.06 | 3.74 | 2.91 | 0.37 | 0.59 |
Endosome | -5.00 | -3.38 | -4.05 | -2.32 | -3.56 | 0.17 | -2.50 | 1.55 | 2.19 | 3.27 | -5.00 | -3.15 | -3.21 | 1.91 | -0.22 |
Golgi | -5.44 | -5.14 | -3.91 | -2.98 | -3.48 | -0.05 | 0.66 | -3.98 | -5.69 | -6.69 | -3.73 | -2.78 | -7.78 | -6.08 | -7.05 |
Lipid Particles | -1.00 | -5.91 | -1.37 | -0.79 | 1.11 | 0.90 | -1.19 | 0.90 | 0.06 | 1.99 | 0.48 | -1.31 | 0.38 | -0.35 | 3.02 |
Mitochondria | 0.56 | 1.93 | 0.10 | -0.23 | -0.79 | 0.75 | 0.14 | 0.44 | -0.44 | 0.22 | 0.03 | 2.34 | -0.82 | -0.87 | -2.90 |
Mitotic Spindle | -3.46 | -3.73 | -2.89 | -2.65 | -1.79 | -1.95 | -1.18 | -2.81 | -1.56 | 0.79 | -3.11 | -2.71 | -3.61 | -2.23 | -1.07 |
None | 0.16 | 0.40 | 0.78 | 1.28 | 1.67 | -2.63 | -2.44 | -1.89 | 1.54 | 2.24 | 0.34 | -0.17 | 0.74 | 1.96 | 4.13 |
Nuclear Periphery | 0.43 | -5.55 | -1.60 | -1.62 | -0.25 | -4.12 | -1.90 | -2.86 | -0.64 | 1.35 | -4.74 | -5.08 | -3.45 | -1.55 | 0.14 |
Nuclear Periphery Foci | -2.08 | -8.22 | -3.86 | -2.74 | -0.90 | -3.57 | -2.36 | -3.43 | 0.22 | 1.53 | -4.28 | -6.88 | -4.21 | -0.62 | -0.42 |
Nucleolus | -1.12 | -11.27 | -1.65 | -1.45 | 3.66 | -4.74 | -6.42 | -3.67 | -0.09 | 6.14 | -3.89 | -12.87 | -3.56 | -1.03 | 10.47 |
Nucleus | -1.21 | -9.20 | -1.89 | -1.70 | 2.17 | -1.41 | -5.76 | -1.77 | -0.85 | 5.62 | -2.45 | -9.73 | -2.59 | -1.42 | 7.19 |
Peroxisomes | -2.36 | -3.35 | -1.11 | -1.01 | -1.01 | 2.04 | -3.73 | 2.15 | 0.54 | 4.51 | -0.32 | -4.30 | -0.74 | -0.66 | 0.84 |
Vacuole | -4.49 | -25.94 | -11.77 | -10.08 | -4.87 | 0.45 | -8.14 | -16.39 | -22.60 | -1.11 | -5.72 | -26.38 | -29.10 | -25.40 | -7.86 |
Vacuole Periphery | 6.42 | 26.53* | 13.72 | 10.88 | 6.50 | -0.69 | 15.77 | 14.57 | 20.87 | -7.80 | 7.19 | 28.18 | 29.29 | 23.40 | 5.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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