Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of protein N-terminal acetyltransferase NatA; NatA comprises Nat1p, Ard1p, Nat5p; acetylates many proteins to influence telomeric silencing, cell cycle, heat-shock resistance, mating, sporulation, early stages of mitophagy; protein abundance increases under DNA replication stress; mutations in human homolog X-linked NAA10 lead to Ogden syndrome (S37P) and intellectual disability (R116W); expression of human NAA10 and NAA15 can complement ard1 nat1 double mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 0 | 3 | 1 | 4 | – | 6 | 8 | 8 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 5 | 2 | – | 8 | 5 | 7 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 11 | 9 | 4 | 15 | 14 | 18 | – | 19 | 29 | 18 | 29 | 6 | 3 | 14 | 8 | 10 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 193 | 379 | 385 | 272 | 311 | 371 | 391 | – | 411 | 429 | 408 | 426 | 229 | 247 | 303 | 114 | 130 | 96 | 190 | 368 | 395 | 200 | 277 | 252 |
Endoplasmic Reticulum | 2 | 7 | 9 | 8 | 14 | 9 | 14 | – | 15 | 13 | 6 | 22 | 4 | 3 | 6 | 4 | 3 | 5 | 3 | 2 | 5 | 10 | 18 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 3 | 10 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
Golgi | 1 | 0 | 0 | 1 | 3 | 0 | 1 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 0 | 0 | 1 | 4 | 3 | 85 | 37 | – | 136 | 184 | 155 | 167 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 4 | 1 | 2 | 2 |
Nucleus | 1 | 3 | 2 | 0 | 4 | 2 | 4 | – | 4 | 5 | 0 | 3 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 3 | 2 | 1 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 3 | 6 | 16 | 15 | 32 | 62 | – | 51 | 63 | 50 | 41 | 0 | 0 | 4 | 3 | 6 | 8 | 0 | 6 | 5 | 2 | 1 | 6 |
Unique Cell Count | 198 | 391 | 401 | 296 | 348 | 449 | 492 | 573 | 648 | 574 | 620 | 232 | 250 | 311 | 120 | 140 | 111 | 205 | 388 | 417 | 221 | 308 | 282 | |
Labelled Cell Count | 202 | 406 | 412 | 305 | 369 | 521 | 535 | 654 | 750 | 656 | 721 | 239 | 254 | 329 | 131 | 153 | 126 | 205 | 388 | 417 | 221 | 308 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.6 | 12.0 | 11.4 | 10.7 | 10.2 | 6.9 | 8.2 | – | 7.3 | 7.2 | 7.2 | 7.1 | 13.2 | 11.6 | 13.2 | 17.5 | 18.1 | 17.7 | 12.2 | 12.9 | 13.0 |
Std Deviation (1e-4) | 2.3 | 2.1 | 1.6 | 1.3 | 1.6 | 1.5 | 1.6 | – | 1.3 | 1.1 | 1.1 | 1.2 | 2.2 | 1.6 | 2.0 | 3.8 | 3.8 | 3.9 | 2.5 | 2.2 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.16 | -0.72 | -0.47 | – | -0.65 | -0.66 | -0.66 | -0.69 | 0.21 | 0.03 | 0.21 | 0.62 | 0.67 | 0.64 | 0.1 | 0.17 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | 1.6 | 0.8 | 1.2 | – | 1.0 | 1.9 | 0.8 | 2.0 | 0.3 | 0 | 1.7 | 0 | 0 | 0 |
Cytoplasm | -2.3 | -3.5 | -6.2 | -7.3 | – | -9.7 | -11.3 | -9.8 | -10.5 | 1.9 | 2.1 | 1.0 | -0.5 | -1.5 | -3.7 |
Endoplasmic Reticulum | 0.4 | 1.4 | -0.2 | 0.6 | – | 0.4 | -0.3 | -1.5 | 1.2 | 0 | 0 | -0.3 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 9.0 | 5.4 | – | 10.4 | 11.6 | 11.2 | 11.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.9 | 2.3 | 4.0 | 6.2 | – | 4.8 | 5.2 | 4.8 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0647 | 7.7634 | 6.9088 | 5.924 | 5.6223 | 7.1159 | 6.2399 | 10.1429 | 9.3233 | 6.9686 | 8.7559 | 8.8344 | 12.1675 | 13.9279 | 13.0839 | 12.0437 | 12.7479 | 13.2297 |
Actin | 0.0204 | 0.0003 | 0.0079 | 0.0128 | 0.0065 | 0.0005 | 0.0228 | 0.0004 | 0.0025 | 0.0067 | 0.0145 | 0.0031 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0005 |
Bud | 0.0006 | 0.0003 | 0.0014 | 0.0006 | 0.0019 | 0.0004 | 0.0008 | 0.0004 | 0.0004 | 0.0008 | 0.0042 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0016 | 0.0003 | 0.0003 |
Bud Neck | 0.0011 | 0.0002 | 0.0004 | 0.0008 | 0.0009 | 0.0008 | 0.0011 | 0.0003 | 0.0003 | 0.0005 | 0.0026 | 0.0007 | 0.0003 | 0.0003 | 0.0003 | 0.0006 | 0.0004 | 0.0008 |
Bud Periphery | 0.0006 | 0 | 0.0008 | 0.0003 | 0.0019 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0006 | 0.0038 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Bud Site | 0.0026 | 0.0003 | 0.0018 | 0.0007 | 0.0014 | 0.0001 | 0.0022 | 0.0013 | 0.0005 | 0.0008 | 0.0105 | 0.0002 | 0.0003 | 0.0043 | 0.0003 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 |
Cytoplasm | 0.7357 | 0.9476 | 0.8534 | 0.8569 | 0.7747 | 0.8772 | 0.6376 | 0.9552 | 0.8859 | 0.702 | 0.8312 | 0.847 | 0.8777 | 0.9459 | 0.9236 | 0.9031 | 0.9063 | 0.9125 |
Cytoplasmic Foci | 0.0206 | 0.0076 | 0.0208 | 0.0123 | 0.0296 | 0.0163 | 0.0585 | 0.0076 | 0.0193 | 0.0499 | 0.035 | 0.0264 | 0.0108 | 0.0087 | 0.0106 | 0.0134 | 0.0116 | 0.0142 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0396 | 0.0063 | 0.0103 | 0.0099 | 0.0051 | 0.0183 | 0.0369 | 0.0064 | 0.0095 | 0.0084 | 0.0024 | 0.0138 | 0.0487 | 0.008 | 0.0178 | 0.0067 | 0.0219 | 0.0192 |
Endosome | 0.0792 | 0.0221 | 0.0629 | 0.042 | 0.0997 | 0.0653 | 0.1007 | 0.0129 | 0.0479 | 0.0872 | 0.0416 | 0.0684 | 0.0331 | 0.0154 | 0.0312 | 0.0409 | 0.0327 | 0.0324 |
Golgi | 0.0146 | 0.0014 | 0.0077 | 0.0076 | 0.0193 | 0.0051 | 0.0248 | 0.0021 | 0.005 | 0.0363 | 0.0247 | 0.0073 | 0.0027 | 0.0017 | 0.0016 | 0.0007 | 0.0016 | 0.0041 |
Lipid Particles | 0.0056 | 0 | 0.0029 | 0.0127 | 0.0152 | 0.0001 | 0.0266 | 0.0002 | 0.0035 | 0.0041 | 0.0008 | 0.0054 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Mitochondria | 0.026 | 0.0008 | 0.0078 | 0.0073 | 0.0176 | 0.0048 | 0.0194 | 0.007 | 0.0015 | 0.0661 | 0.0119 | 0.0021 | 0.0012 | 0.0007 | 0.0005 | 0.0003 | 0.0006 | 0.0037 |
None | 0.0063 | 0.0007 | 0.0017 | 0.0007 | 0.0008 | 0.0007 | 0.0067 | 0.0009 | 0.001 | 0.0028 | 0.0005 | 0.0008 | 0.007 | 0.0066 | 0.0014 | 0.0009 | 0.0009 | 0.0054 |
Nuclear Periphery | 0.0125 | 0.0038 | 0.0027 | 0.0059 | 0.0013 | 0.0008 | 0.0213 | 0.0007 | 0.0014 | 0.0034 | 0.0008 | 0.0064 | 0.0032 | 0.0005 | 0.0016 | 0.0016 | 0.002 | 0.0008 |
Nucleolus | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Nucleus | 0.0034 | 0.0013 | 0.0013 | 0.0019 | 0.0011 | 0.0008 | 0.0053 | 0.0008 | 0.0008 | 0.0015 | 0.0015 | 0.0013 | 0.0015 | 0.001 | 0.0011 | 0.0017 | 0.0014 | 0.0008 |
Peroxisomes | 0.0033 | 0 | 0.0023 | 0.0113 | 0.0117 | 0.0001 | 0.0174 | 0.0001 | 0.0096 | 0.003 | 0.003 | 0.0075 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.004 | 0.0001 | 0.0009 | 0.0012 | 0.0005 | 0.0001 | 0.0035 | 0.0001 | 0.0002 | 0.0039 | 0.0003 | 0.0012 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0154 | 0.0053 | 0.0095 | 0.0112 | 0.0068 | 0.0061 | 0.009 | 0.003 | 0.0081 | 0.0178 | 0.0083 | 0.0051 | 0.0099 | 0.0045 | 0.0073 | 0.0231 | 0.0169 | 0.0034 |
Vacuole Periphery | 0.0078 | 0.0018 | 0.0033 | 0.0034 | 0.0035 | 0.0026 | 0.0039 | 0.0005 | 0.0021 | 0.0036 | 0.002 | 0.0028 | 0.0018 | 0.0007 | 0.0017 | 0.0046 | 0.0029 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 72.3292 | 46.7738 | 63.2913 | 64.4283 | 74.8228 | 59.9846 | 58.4206 | 53.0427 | 58.071 | 68.8896 |
Translational Efficiency | 1.7115 | 2.4152 | 1.5317 | 1.7368 | 1.5998 | 2.029 | 1.816 | 1.4189 | 1.3991 | 1.5948 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
989 | 1488 | 305 | 1592 | 1836 | 2319 | 320 | 1109 | 2825 | 3807 | 625 | 2701 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 814.08 | 1121.45 | 1548.97 | 1605.55 | 909.76 | 1159.26 | 1578.68 | 1525.29 | 876.26 | 1144.48 | 1564.18 | 1572.60 |
Standard Deviation | 109.55 | 202.43 | 204.21 | 309.02 | 132.78 | 183.85 | 208.35 | 288.78 | 133.20 | 192.22 | 206.87 | 303.45 |
Intensity Change Log 2 | — | 0.462123 | 0.928067 | 0.979825 | — | 0.349646 | 0.795161 | 0.745526 | — | 0.403857 | 0.859453 | 0.860924 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000187 | 0.000263 | 0.000768 | 0.000934 | 0.000106 | 0.000254 | 0.000868 | 0.000804 | 0.000134 | 0.000258 | 0.000819 | 0.000880 |
Bud Neck | 0.003674 | 0.000577 | 0.003197 | 0.001210 | 0.001135 | 0.000567 | 0.002086 | 0.001638 | 0.002024 | 0.000571 | 0.002628 | 0.001385 |
Bud Site | 0.029020 | 0.025203 | 0.033917 | 0.027125 | 0.016268 | 0.020197 | 0.029026 | 0.039272 | 0.020732 | 0.022154 | 0.031413 | 0.032112 |
Cell Periphery | 0.000144 | 0.000304 | 0.000414 | 0.001541 | 0.000100 | 0.000094 | 0.000501 | 0.000857 | 0.000115 | 0.000176 | 0.000459 | 0.001260 |
Cytoplasm | 0.605398* | 0.812845* | 0.631626* | 0.857121* | 0.731618* | 0.814693* | 0.795941* | 0.763030* | 0.687430* | 0.813971* | 0.715755* | 0.818488* |
Cytoplasmic Foci | 0.250378* | 0.106647 | 0.107768 | 0.024198 | 0.166682 | 0.108347 | 0.064261 | 0.054708 | 0.195983* | 0.107683 | 0.085493 | 0.036725 |
Eisosomes | 0.000046 | 0.000005 | 0.000007 | 0.000006 | 0.000010 | 0.000006 | 0.000005 | 0.000008 | 0.000023 | 0.000005 | 0.000006 | 0.000007 |
Endoplasmic Reticulum | 0.002557 | 0.001678 | 0.004764 | 0.000520 | 0.002483 | 0.001292 | 0.000773 | 0.001340 | 0.002509 | 0.001443 | 0.002721 | 0.000857 |
Endosome | 0.014003 | 0.006388 | 0.026419 | 0.003841 | 0.012438 | 0.005218 | 0.007443 | 0.012679 | 0.012986 | 0.005675 | 0.016703 | 0.007470 |
Golgi | 0.025789 | 0.012984 | 0.014999 | 0.012938 | 0.012405 | 0.013681 | 0.009277 | 0.023840 | 0.017090 | 0.013409 | 0.012070 | 0.017414 |
Lipid Particles | 0.001449 | 0.000749 | 0.003774 | 0.005667 | 0.001017 | 0.000374 | 0.004104 | 0.004259 | 0.001168 | 0.000521 | 0.003943 | 0.005089 |
Mitochondria | 0.005256 | 0.000880 | 0.000042 | 0.000626 | 0.001002 | 0.001567 | 0.000015 | 0.003480 | 0.002491 | 0.001299 | 0.000028 | 0.001798 |
Mitotic Spindle | 0.000478 | 0.001528 | 0.011573 | 0.002239 | 0.000268 | 0.002404 | 0.004751 | 0.006231 | 0.000341 | 0.002061 | 0.008080 | 0.003878 |
None | 0.001010 | 0.000833 | 0.000397 | 0.000199 | 0.000876 | 0.000594 | 0.000144 | 0.000202 | 0.000923 | 0.000687 | 0.000268 | 0.000200 |
Nuclear Periphery | 0.000032 | 0.000021 | 0.000187 | 0.000050 | 0.000073 | 0.000016 | 0.000041 | 0.000128 | 0.000059 | 0.000018 | 0.000112 | 0.000082 |
Nuclear Periphery Foci | 0.000246 | 0.000384 | 0.005775 | 0.001260 | 0.000297 | 0.000141 | 0.003047 | 0.001985 | 0.000279 | 0.000236 | 0.004378 | 0.001558 |
Nucleolus | 0.000444 | 0.000062 | 0.000061 | 0.000106 | 0.000096 | 0.000041 | 0.000049 | 0.000108 | 0.000218 | 0.000049 | 0.000055 | 0.000107 |
Nucleus | 0.006635 | 0.002305 | 0.003784 | 0.002625 | 0.004517 | 0.001853 | 0.004557 | 0.002957 | 0.005259 | 0.002030 | 0.004179 | 0.002761 |
Peroxisomes | 0.001417 | 0.000535 | 0.000710 | 0.000670 | 0.000619 | 0.000536 | 0.000476 | 0.001371 | 0.000898 | 0.000535 | 0.000590 | 0.000958 |
Vacuole | 0.036346 | 0.018702 | 0.135322 | 0.049607 | 0.037782 | 0.018148 | 0.067970 | 0.068227 | 0.037279 | 0.018365 | 0.100838 | 0.057252 |
Vacuole Periphery | 0.015489 | 0.007109 | 0.014495 | 0.007517 | 0.010208 | 0.009979 | 0.004666 | 0.012876 | 0.012057 | 0.008857 | 0.009463 | 0.009718 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.46 | -5.70 | -2.63 | -2.32 | -0.55 | -2.46 | -4.63 | -5.51 | -3.91 | 0.41 | -2.94 | -7.00 | -4.23 | -3.44 | -0.26 |
Bud Neck | 9.82 | 0.90 | 7.26 | -6.88 | 6.83 | 4.99 | -4.06 | -3.65 | -8.13 | 2.01 | 10.80 | -3.41 | 3.51 | -10.51 | 6.19 |
Bud Site | 1.78 | -1.41 | 0.88 | -0.79 | 2.01 | -3.05 | -4.40 | -8.24 | -6.69 | -2.25 | -1.28 | -4.90 | -6.69 | -5.82 | 0.00 |
Cell Periphery | -1.82 | -7.02 | -6.16 | -5.04 | -4.86 | 1.03 | -7.63 | -9.20 | -9.28 | -3.52 | -1.76 | -10.46 | -8.34 | -7.64 | -5.59 |
Cytoplasm | -20.10 | -1.39 | -24.72 | -5.06 | -13.01 | -10.96 | -4.26 | -2.75 | 5.70 | 1.97 | -20.39 | -2.08 | -18.48 | -0.07 | -8.93 |
Cytoplasmic Foci | 18.64 | 15.92 | 33.01 | 20.38 | 13.80 | 10.92 | 16.51 | 21.25 | 12.24 | 1.79 | 19.83 | 20.77 | 39.52 | 24.84 | 12.13 |
Eisosomes | 7.01 | 6.64 | 6.78 | -0.61 | 0.78 | 5.52 | 5.94 | 2.24 | -2.47 | -2.75 | 8.13 | 7.70 | 7.17 | -1.68 | -0.74 |
Endoplasmic Reticulum | 1.45 | -1.22 | 3.68 | 3.86 | 2.57 | 2.63 | 4.41 | 2.42 | 0.04 | -1.39 | 2.95 | -0.15 | 4.93 | 2.44 | 2.27 |
Endosome | 7.78 | -5.39 | 11.07 | 3.97 | 10.51 | 8.75 | 4.21 | 0.20 | -5.45 | -3.06 | 11.46 | -2.74 | 7.26 | -2.42 | 7.18 |
Golgi | 9.58 | 6.50 | 7.40 | 0.44 | 1.19 | -1.46 | 2.80 | -4.14 | -3.53 | -5.12 | 4.98 | 5.34 | 0.42 | -2.22 | -3.11 |
Lipid Particles | 5.25 | -6.48 | -6.64 | -7.67 | -2.53 | 9.79 | -6.07 | -7.90 | -9.48 | -0.02 | 9.85 | -8.80 | -9.58 | -11.12 | -1.99 |
Mitochondria | 8.02 | 11.38 | 9.57 | 0.86 | -3.29 | -1.93 | 7.77 | -2.00 | -1.47 | -2.90 | 4.41 | 13.36 | 1.60 | -0.75 | -3.52 |
Mitotic Spindle | -1.67 | -5.60 | -3.34 | -0.95 | 4.49 | -3.76 | -2.90 | -4.48 | -2.65 | -0.68 | -4.05 | -6.14 | -5.64 | -2.45 | 2.93 |
None | 0.45 | 2.03 | 4.99 | 1.74 | 0.68 | 0.92 | 3.01 | 2.67 | 1.91 | -1.46 | 0.96 | 3.13 | 4.26 | 2.60 | 0.41 |
Nuclear Periphery | 1.40 | -5.09 | -0.75 | -1.19 | 3.48 | 2.03 | 0.77 | -1.43 | -4.00 | -2.67 | 2.21 | -2.44 | -0.95 | -3.45 | 1.38 |
Nuclear Periphery Foci | -2.05 | -5.20 | -3.96 | -3.50 | 4.07 | 3.11 | -8.32 | -3.22 | -3.50 | 1.74 | 1.08 | -7.64 | -4.87 | -5.03 | 4.61 |
Nucleolus | 1.28 | 1.28 | 1.13 | -4.21 | -5.21 | 6.02 | 4.50 | -1.18 | -5.76 | -4.71 | 1.61 | 1.53 | 1.03 | -8.10 | -7.18 |
Nucleus | 5.99 | 3.18 | 4.90 | -3.02 | 1.95 | 7.22 | -0.82 | 1.90 | -4.35 | 2.39 | 9.22 | 1.55 | 5.13 | -5.93 | 3.29 |
Peroxisomes | 3.12 | 2.46 | 2.68 | -1.53 | 0.59 | 1.46 | 1.76 | -1.84 | -2.05 | -2.14 | 3.42 | 2.68 | -0.25 | -2.41 | -1.99 |
Vacuole | 6.80 | -9.06 | -5.29 | -11.88 | 7.34 | 9.44 | -4.56 | -8.51 | -14.41 | 0.02 | 11.61 | -9.82 | -9.49 | -18.69 | 6.02 |
Vacuole Periphery | 5.85 | 0.73 | 5.57 | -0.05 | 3.70 | 0.21 | 3.84 | -1.43 | -1.56 | -4.38 | 3.76 | 2.36 | 3.03 | -0.45 | -0.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|