Standard name | |
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Human Ortholog | |
Description | Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 2 | 4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 5 | 3 | 4 | 9 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 2 | 7 |
Bud Neck | 1 | 2 | 3 | 0 | 5 | 7 | 14 | – | 6 | 14 | 4 | 8 | 1 | 0 | 1 | 1 | 4 | – | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 4 | 0 | 9 | 9 | 6 | – | 1 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 147 | 156 | 44 | 115 | 218 | 203 | 280 | – | 217 | 290 | 85 | 163 | 158 | 264 | 349 | 349 | 365 | – | 134 | 133 | 39 | 43 | 203 | 235 |
Endoplasmic Reticulum | 2 | 2 | 0 | 1 | 2 | 0 | 1 | – | 0 | 0 | 8 | 2 | 0 | 1 | 2 | 35 | 35 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | – | 2 | 2 | 1 | 1 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 15 | 0 | 0 | 2 | 2 | 8 | 24 | – | 50 | 62 | 73 | 54 | 0 | 0 | 0 | 8 | 6 | – | 18 | 11 | 7 | 1 | 6 | 5 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 2 | 2 | 0 | 1 | 1 | 0 | 0 | 3 | 3 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 1 | 9 | – | 12 | 8 | 4 | 11 | 0 | 0 | 0 | 2 | 3 | – | 1 | 2 | 1 | 0 | 2 | 3 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 5 | 4 | – | 4 | 5 | 1 | 1 | 3 | 2 |
Unique Cell Count | 154 | 161 | 44 | 118 | 223 | 213 | 307 | 256 | 341 | 155 | 218 | 159 | 266 | 350 | 364 | 383 | 169 | 163 | 56 | 51 | 236 | 273 | ||
Labelled Cell Count | 165 | 164 | 48 | 120 | 231 | 222 | 330 | 299 | 381 | 179 | 251 | 165 | 266 | 362 | 415 | 437 | 169 | 163 | 56 | 51 | 236 | 273 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 4.1 | 4.5 | 4.9 | 4.9 | 3.9 | 4.0 | – | 3.5 | 3.6 | 3.0 | 3.3 | 4.9 | 4.9 | 4.8 | 5.3 | 5.5 | 4.3 | – | 4.6 | 4.8 |
Std Deviation (1e-4) | 0.5 | 0.7 | 0.7 | 1.9 | 1.3 | 1.0 | 1.2 | – | 1.2 | 1.1 | 0.5 | 0.7 | 1.0 | 1.4 | 1.1 | 1.4 | 2.0 | 1.1 | – | 1.1 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.14 | -0.2 | -0.17 | – | -0.35 | -0.33 | -0.58 | -0.44 | 0.14 | 0.14 | 0.09 | 0.26 | 0.3 | -0.05 | – | 0.03 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.6737 | -1.5133 | -1.4459 | -1.8773 | -1.1112 | -1.5234 | -0.9404 | -0.6439 | -1.0332 | -1.1217 | -0.4848 | -0.6729 | -2.2257 | -2.0215 | -1.9645 | -1.7589 | -1.5072 | -1.9284 |
Actin | 0.0083 | 0.0007 | 0.0158 | 0.0049 | 0.0012 | 0.0006 | 0.0264 | 0.0004 | 0.0006 | 0.0231 | 0.0252 | 0.001 | 0.0124 | 0.0016 | 0.0085 | 0.0158 | 0.0134 | 0.0032 |
Bud | 0.0013 | 0 | 0.0013 | 0.0014 | 0 | 0 | 0.0008 | 0.0015 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0009 | 0.0002 | 0.0007 | 0.0005 | 0.0013 | 0.0005 |
Bud Neck | 0.0007 | 0.0001 | 0.0009 | 0.0001 | 0.0009 | 0.0018 | 0.0009 | 0 | 0.0002 | 0.0006 | 0.0003 | 0.0233 | 0.0014 | 0.0001 | 0.0007 | 0.0007 | 0.0018 | 0.0007 |
Bud Periphery | 0.0016 | 0 | 0.0043 | 0.0009 | 0 | 0 | 0.0016 | 0.004 | 0.0007 | 0.0001 | 0.0011 | 0.0004 | 0.0013 | 0.0004 | 0.0015 | 0.0006 | 0.003 | 0.0031 |
Bud Site | 0.0033 | 0.0003 | 0.0057 | 0.0011 | 0.0001 | 0 | 0.0067 | 0.0006 | 0.0007 | 0.0006 | 0.0018 | 0.0116 | 0.0041 | 0.0009 | 0.0044 | 0.0024 | 0.0033 | 0.0002 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0012 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0203 | 0.0268 | 0.0142 | 0.0253 | 0.0393 | 0.0528 | 0.0172 | 0.0313 | 0.0314 | 0.033 | 0.0092 | 0.0281 | 0.0264 | 0.0294 | 0.0304 | 0.0554 | 0.0215 | 0.0887 |
Cytoplasmic Foci | 0.0314 | 0.0051 | 0.0191 | 0.0084 | 0.0408 | 0.0133 | 0.0492 | 0.0128 | 0.0093 | 0.0118 | 0.0165 | 0.0293 | 0.0301 | 0.0073 | 0.0139 | 0.017 | 0.0195 | 0.0154 |
Eisosomes | 0.0009 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0026 | 0.0003 | 0.0012 | 0.0019 | 0.004 | 0.0002 | 0.0058 | 0.0012 | 0.0003 | 0.0005 | 0.0008 | 0.0002 | 0.0027 | 0.0019 | 0.0022 | 0.001 | 0.0005 | 0.002 |
Endosome | 0.0162 | 0.0013 | 0.0123 | 0.0249 | 0.0093 | 0.0041 | 0.019 | 0.0016 | 0.0006 | 0.0021 | 0.0344 | 0.0002 | 0.0292 | 0.0056 | 0.0231 | 0.0079 | 0.0165 | 0.0162 |
Golgi | 0.004 | 0.0049 | 0.0118 | 0.0129 | 0.0023 | 0.0059 | 0.0096 | 0.0004 | 0.0004 | 0.0051 | 0.0198 | 0.0001 | 0.0044 | 0.0045 | 0.0032 | 0.0123 | 0.0209 | 0.0102 |
Lipid Particles | 0.0263 | 0.0014 | 0.0126 | 0.0001 | 0.0327 | 0.0008 | 0.0127 | 0.0001 | 0.003 | 0.0036 | 0.0187 | 0.0002 | 0.0093 | 0.0008 | 0.0046 | 0.0089 | 0.0204 | 0.0018 |
Mitochondria | 0.0069 | 0.0069 | 0.0054 | 0.0284 | 0.0004 | 0.0053 | 0.0165 | 0.003 | 0.0017 | 0.0008 | 0.0278 | 0.0001 | 0.0245 | 0.0081 | 0.0035 | 0.0588 | 0.1338 | 0.0093 |
None | 0.8447 | 0.9498 | 0.8805 | 0.886 | 0.8277 | 0.9127 | 0.7914 | 0.938 | 0.9387 | 0.9035 | 0.7881 | 0.9034 | 0.8329 | 0.9363 | 0.8833 | 0.7738 | 0.711 | 0.838 |
Nuclear Periphery | 0.0018 | 0.0001 | 0.0019 | 0.0007 | 0.0054 | 0.0001 | 0.0056 | 0.0007 | 0.0002 | 0.0002 | 0.001 | 0.0001 | 0.0038 | 0.0004 | 0.0018 | 0.0004 | 0.0004 | 0.0044 |
Nucleolus | 0.0019 | 0.0002 | 0.0007 | 0 | 0.0009 | 0.0001 | 0.0027 | 0.0001 | 0.0004 | 0.0001 | 0.001 | 0.0001 | 0.001 | 0.0001 | 0.0022 | 0.0001 | 0.0004 | 0.0002 |
Nucleus | 0.0022 | 0.0001 | 0.0008 | 0.0006 | 0.0058 | 0.0003 | 0.0044 | 0.0003 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0011 | 0.0002 | 0.0014 | 0.0004 | 0.0003 | 0.0009 |
Peroxisomes | 0.014 | 0.0005 | 0.0083 | 0.001 | 0.0209 | 0.0007 | 0.0065 | 0.0005 | 0.0058 | 0.0136 | 0.0376 | 0.0002 | 0.0074 | 0.0009 | 0.0046 | 0.041 | 0.0283 | 0.0003 |
Punctate Nuclear | 0.0086 | 0.0003 | 0.0014 | 0.0002 | 0.0071 | 0.0006 | 0.0045 | 0.0004 | 0.0051 | 0.0003 | 0.0023 | 0.0006 | 0.0027 | 0.0004 | 0.0024 | 0.0004 | 0.0005 | 0.0007 |
Vacuole | 0.0023 | 0.0002 | 0.0009 | 0.0005 | 0.0007 | 0.0002 | 0.0134 | 0.0018 | 0.0002 | 0.0004 | 0.0094 | 0.0002 | 0.0031 | 0.0007 | 0.0057 | 0.0017 | 0.0016 | 0.0026 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0029 | 0.0007 | 0.0001 | 0.0001 | 0.0028 | 0 | 0.0007 | 0.0002 | 0.0016 | 0.0008 | 0.0013 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 0.8359 | 0.9963 | 3.6966 | 1.3768 | 1.8063 | 2.7848 | 0.8815 | 0.8499 | 0.7986 | 1.4221 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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