Standard name
Human Ortholog
Description Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.96 0.98 1.0 0.97 0.91 0.87 0.9 0.86 0.81 0.84 1.0 1.0 0.99 0.98 0.96 0.98 0.92 0.93 0.93 0.93 0.92 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.08 0.08 0.09 0.06 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 1 0 2 4 3 0 0 0 0 0 0 1 0 0 0 1 2
Bud 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 1 0 0 1 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 3 1 2 3 4 7 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 239 138 27 52 131 146 152 181 107 147 95 138 341 462 567 122 143 113 222 123 56 205 295 292
Endoplasmic Reticulum 1 0 1 0 0 2 9 16 9 15 7 7 2 0 2 8 9 3 1 0 0 0 1 15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 3
Golgi 1 0 0 0 0 0 0 1 0 1 4 2 0 0 0 2 2 0 0 0 0 0 0 6
Mitochondria 0 0 0 1 0 3 1 1 3 5 7 6 0 0 0 0 1 0 0 0 0 0 0 0
Nucleus 2 0 0 0 0 1 0 1 1 0 0 2 0 0 0 0 0 0 3 2 0 3 3 16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 4 0 0 0 0 0 5 7 0 5 5 3 0 2 2 2 1 1 1 3 0 2 5 17
Unique Cell Count 247 138 28 53 131 151 167 208 119 171 117 165 342 463 573 125 149 115 242 133 60 220 320 373
Labelled Cell Count 247 138 28 53 132 152 173 213 122 178 126 170 343 464 573 135 158 120 242 133 60 220 320 373


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 97.3 100.8 75.5 62.2 68.5 55.5 50.5 39.8 41.4 40.6 34.1 34.1 96.1 91.2 87.8 108.7 127.6 152.4 90.1 86.1 91.8
Std Deviation (1e-4) 28.6 28.0 14.2 18.4 18.5 17.8 16.0 15.8 14.0 13.5 13.9 11.8 21.5 22.2 18.5 29.1 37.4 24.4 19.8 22.4 24.4
Intensity Change (Log2) – – – -0.28 -0.14 -0.44 -0.58 -0.93 -0.87 -0.9 -1.15 -1.15 0.35 0.27 0.22 0.52 0.76 1.01 0.25 0.19 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 2.2 0.1 -1.0 -1.4 -1.1 -1.5 -2.0 -1.8 2.3 2.7 1.2 0.4 -0.1 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 155.1946 198.7815 168.8263 170.8355 174.6779 165.4175 105.1144 146.4622 138.2431 117.9642 91.4621 129.7322 151.3499 183.9711 170.2898 170.4221 174.1193 152.9056
Actin 0.0103 0.0001 0.0024 0.0001 0.0001 0.0006 0.0062 0.0004 0.0009 0.0314 0.0025 0.0092 0.0003 0.0001 0.0038 0.0091 0.0001 0.0007
Bud 0.0008 0.0001 0.0003 0 0.0004 0.0004 0.0005 0.0001 0.0019 0.0004 0.0043 0.0003 0.001 0.0001 0.0003 0.0003 0.0001 0.0002
Bud Neck 0.0007 0.0002 0.0017 0.0002 0.0003 0.001 0.0003 0.0001 0.0002 0.0002 0.0004 0.0008 0.0002 0.0001 0.0002 0.0002 0.0002 0.0014
Bud Periphery 0.0006 0 0.0003 0 0.0001 0.0001 0.0002 0 0.0006 0.0002 0.0008 0.0001 0.0004 0 0.0002 0.0002 0 0
Bud Site 0.0003 0.0001 0.005 0 0.0001 0 0.0009 0.0004 0.0002 0.0001 0.0004 0.0003 0.0001 0 0.0016 0.0009 0 0
Cell Periphery 0.0007 0.0015 0.0008 0.0002 0.0003 0.0003 0.0004 0 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0 0.0002
Cytoplasm 0.8292 0.973 0.8993 0.9099 0.9163 0.8538 0.8002 0.9915 0.9692 0.876 0.6895 0.9098 0.9121 0.9772 0.919 0.867 0.9761 0.9428
Cytoplasmic Foci 0.0062 0.0001 0.0071 0.0001 0.0006 0.0005 0.0051 0.0001 0.0016 0.0004 0.0015 0.0013 0.0052 0.0001 0.0146 0.0282 0.0001 0.0004
Eisosomes 0.0008 0 0.0002 0 0 0 0.0003 0 0 0.0001 0 0.0001 0 0 0.0001 0.0011 0 0
Endoplasmic Reticulum 0.001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0021 0.0001 0.0001 0.0043 0.0005 0.0057 0.0002 0.0001 0.0006 0.0005 0.0001 0.0003
Endosome 0.0021 0 0.0029 0 0.0004 0.0002 0.0019 0 0.0003 0.0035 0.0007 0.0103 0.0002 0 0.0028 0.0031 0 0.0001
Golgi 0.0007 0 0.0013 0 0 0 0.0008 0 0 0.0012 0 0.0007 0 0 0.001 0.0025 0 0
Lipid Particles 0.0026 0 0.0058 0 0 0.0001 0.0022 0 0.0001 0.0001 0 0.0005 0.0006 0 0.0033 0.015 0 0.0001
Mitochondria 0.0056 0.0003 0.0036 0.0003 0.003 0.001 0.0044 0.0001 0.0034 0.0031 0.0587 0.0013 0.0016 0.0003 0.0041 0.0169 0.0001 0.0002
None 0.0181 0.0002 0.0026 0.0003 0.0005 0.0012 0.0044 0.0001 0.0017 0.0008 0.0057 0.006 0.0026 0.0006 0.0044 0.024 0.0002 0.0004
Nuclear Periphery 0.0138 0.0034 0.0067 0.014 0.0098 0.0239 0.0284 0.0009 0.0015 0.0224 0.0407 0.0218 0.0072 0.0019 0.0037 0.0019 0.0033 0.0123
Nucleolus 0.0053 0.0003 0.0063 0.0005 0.0014 0.0024 0.0052 0 0.0008 0.0002 0.0016 0.0005 0.0047 0.0005 0.0028 0.0077 0 0.0003
Nucleus 0.0896 0.0185 0.0337 0.0719 0.0496 0.1083 0.1243 0.0058 0.0128 0.0533 0.1847 0.0227 0.0604 0.0179 0.031 0.0132 0.019 0.0388
Peroxisomes 0.001 0 0.0137 0 0.0001 0 0.0003 0 0.0002 0.0001 0.0006 0.0002 0.0001 0 0.0012 0.0034 0 0
Punctate Nuclear 0.0063 0.0001 0.003 0.0002 0.0002 0.001 0.0072 0.0001 0.0001 0.0005 0.0012 0.0066 0.0007 0.0001 0.0026 0.0012 0.0001 0.0007
Vacuole 0.0026 0.0017 0.0019 0.0011 0.0078 0.0026 0.0023 0.0001 0.0031 0.0006 0.0021 0.0009 0.0015 0.0005 0.0016 0.0014 0.0003 0.0007
Vacuole Periphery 0.0018 0.0004 0.0012 0.0011 0.0089 0.0022 0.0026 0 0.001 0.0012 0.0037 0.0007 0.0007 0.0004 0.001 0.0017 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.9386 46.0592 51.004 69.9686 61.1504 96.6529 97.7979 102.2746 107.5303 84.3273
Translational Efficiency 1.9473 1.9608 1.5951 1.7039 1.8667 1.5011 1.5254 1.4217 1.4677 1.5718

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1077 957 716 1370 139 1608 180 1320 1216 2565 896 2690

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2797.52 7605.52 4335.29 1946.68 5200.37 6979.31 2784.16 1704.59 3072.19 7212.95 4023.68 1827.88
Standard Deviation 608.42 1726.60 797.39 489.24 1054.61 1768.43 736.82 412.14 1019.58 1778.91 1001.70 468.94
Intensity Change Log 2 — 1.442898 0.631980 -0.523133 — 0.424470 -0.901372 -1.609189 — 0.866777 -0.167854 -1.131221

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000213 0.000062 0.000118 0.001901 0.000307 0.000084 0.000419 0.003421 0.000224 0.000076 0.000178 0.002647
Bud Neck 0.000810 0.000480 0.001494 0.004111 0.001307 0.000357 0.003234 0.002769 0.000867 0.000403 0.001844 0.003452
Bud Site 0.003235 0.000378 0.000875 0.013366 0.000368 0.000445 0.002395 0.019862 0.002907 0.000420 0.001181 0.016554
Cell Periphery 0.000556 0.000576 0.000931 0.000163 0.001559 0.000232 0.000880 0.000178 0.000670 0.000360 0.000921 0.000170
Cytoplasm 0.769934* 0.484716* 0.450222* 0.560536* 0.340031* 0.527203* 0.390343* 0.613632* 0.720792* 0.511351* 0.438193* 0.586590*
Cytoplasmic Foci 0.008640 0.005251 0.009075 0.008416 0.012547 0.002205 0.009257 0.012757 0.009087 0.003341 0.009111 0.010546
Eisosomes 0.000001 0.000001 0.000002 0.000007 0.000003 0.000001 0.000026 0.000008 0.000001 0.000001 0.000007 0.000008
Endoplasmic Reticulum 0.000124 0.000017 0.000043 0.000377 0.000064 0.000026 0.000800 0.000530 0.000118 0.000023 0.000195 0.000452
Endosome 0.000257 0.000010 0.000082 0.006885 0.000048 0.000063 0.001418 0.007778 0.000233 0.000044 0.000351 0.007323
Golgi 0.004195 0.000219 0.000586 0.009126 0.002918 0.000331 0.000650 0.004777 0.004049 0.000289 0.000599 0.006992
Lipid Particles 0.000307 0.000123 0.000425 0.001020 0.000410 0.000076 0.001653 0.001307 0.000319 0.000094 0.000672 0.001161
Mitochondria 0.000442 0.000214 0.000404 0.002056 0.000649 0.000223 0.000673 0.001730 0.000465 0.000220 0.000458 0.001896
Mitotic Spindle 0.002689 0.000861 0.002229 0.053005 0.000089 0.001382 0.010441 0.072962 0.002392 0.001188 0.003879 0.062798
None 0.009513 0.014985 0.010550 0.012509 0.004558 0.019955 0.007281 0.012011 0.008947 0.018100 0.009893 0.012265
Nuclear Periphery 0.000137 0.000134 0.000521 0.000489 0.000724 0.000100 0.001487 0.000435 0.000204 0.000112 0.000715 0.000462
Nuclear Periphery Foci 0.001361 0.000315 0.000654 0.001554 0.001302 0.000381 0.002151 0.002362 0.001354 0.000356 0.000955 0.001951
Nucleolus 0.000168 0.000047 0.000140 0.000449 0.000412 0.000223 0.000409 0.000652 0.000196 0.000157 0.000194 0.000549
Nucleus 0.189324* 0.490000* 0.513075* 0.291816* 0.625106* 0.445108* 0.499296* 0.211336* 0.239138* 0.461857* 0.510307* 0.252324*
Peroxisomes 0.000029 0.000002 0.000008 0.000572 0.000007 0.000027 0.000165 0.000804 0.000026 0.000018 0.000040 0.000686
Vacuole 0.002440 0.001550 0.008333 0.026077 0.005924 0.001279 0.066773 0.025662 0.002838 0.001380 0.020073 0.025874
Vacuole Periphery 0.005626 0.000060 0.000233 0.005566 0.001665 0.000299 0.000250 0.005026 0.005173 0.000210 0.000236 0.005301

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.05 -0.19 -1.92 -2.55 -1.90 3.47 -3.91 -2.05 -2.66 -1.02 3.71 -2.23 -2.96 -3.60 -2.55
Bud Neck 0.73 -7.10 -7.94 -8.09 -1.19 4.36 -7.64 -5.86 -12.88 5.02 2.51 -9.54 -10.21 -13.76 1.31
Bud Site 4.02 1.79 -2.94 -6.62 -4.86 -1.53 -7.94 -7.69 -7.43 -3.57 3.86 0.71 -5.15 -9.91 -6.94
Cell Periphery -1.20 -3.10 5.88 6.15 5.08 6.35 2.53 6.90 2.73 6.32 4.08 -2.77 8.68 6.16 6.25
Cytoplasm 29.97 36.65 27.41 -4.49 -11.77 -8.69 1.21 -10.64 -5.40 -16.51 25.09 33.47 20.06 -6.03 -19.41
Cytoplasmic Foci 0.94 -6.26 1.28 0.28 7.86 4.97 -0.92 1.27 -8.29 2.03 4.41 -6.29 0.20 -6.85 7.24
Eisosomes -0.77 -6.63 -9.75 -9.59 -3.30 4.00 -3.39 -4.12 -9.92 2.53 0.86 -6.16 -13.05 -13.39 -0.04
Endoplasmic Reticulum 5.77 2.76 -5.16 -11.31 -8.67 2.91 -5.23 -6.60 -7.76 3.29 5.47 -2.92 -6.71 -12.36 -2.19
Endosome 2.85 1.45 -3.67 -4.45 -4.12 -0.70 -5.41 -4.04 -3.98 -1.88 2.33 -0.95 -5.09 -5.83 -4.93
Golgi 5.53 4.87 2.43 -2.70 -1.96 2.40 1.99 1.55 -2.29 -1.10 5.75 5.14 3.41 -3.40 -2.31
Lipid Particles 2.50 -6.30 -8.90 -10.51 -4.07 3.69 -5.13 -7.99 -10.89 2.54 3.84 -7.75 -12.08 -15.21 -1.71
Mitochondria 4.94 -0.92 -1.96 -3.23 -1.76 4.08 -0.17 0.06 -2.78 0.18 5.33 -1.27 -2.12 -4.22 -1.72
Mitotic Spindle 1.73 -0.10 -8.22 -8.85 -8.09 -2.34 -2.44 -10.69 -10.11 -4.51 1.08 -1.66 -12.76 -13.34 -10.92
None -4.94 0.07 -1.14 3.64 -1.23 -11.58 -1.29 -8.59 7.67 -6.65 -9.10 0.57 -1.68 8.21 -2.41
Nuclear Periphery -1.49 -14.57 -18.72 -18.56 0.69 1.96 -3.84 -0.67 -16.93 6.69 1.86 -14.52 -15.00 -24.92 4.23
Nuclear Periphery Foci 3.89 0.81 -2.08 -7.95 -3.95 2.67 -3.80 -2.46 -7.69 2.32 4.17 -1.01 -2.92 -10.75 -2.71
Nucleolus 3.31 -0.44 -1.94 -2.76 -1.88 0.61 0.26 -0.01 -1.31 -0.74 0.35 -0.46 -2.51 -2.54 -2.53
Nucleus -32.00* -34.53 -15.16 18.65 21.48 7.96 5.45 17.79 29.29 12.19 -27.16 -27.15 -4.04 32.03 28.92
Peroxisomes 2.50 0.84 -2.36 -2.86 -2.56 -1.49 -3.68 -3.98 -3.43 -2.48 0.09 -1.00 -4.35 -4.29 -4.16
Vacuole 0.33 -9.58 -20.81 -20.91 -14.16 3.01 -9.08 -20.39 -22.18 2.81 1.64 -11.35 -30.01 -30.40 -9.32
Vacuole Periphery 3.77 3.66 2.90 -2.65 -2.25 2.29 2.44 0.64 -1.74 -1.92 3.78 3.77 2.98 -2.94 -2.94
Standard name
Human Ortholog
Description Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Arg4

Arg4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available