Standard name
Human Ortholog
Description Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm – 0.5 0.45 0.56 0.59 0.65 0.65 0.76 – 0.72 0.82 0.78 0.35 0.37 0.48 – – – 0 0 0 0 0 0
Endoplasmic Reticulum – 0.46 0.45 0.44 0.42 0.34 0.29 0.14 – 0.15 0.14 0.13 0.62 0.58 0.39 – – – 0.66 0.62 0.65 0.67 0.65 0.58
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0.13 0.2 0.06 0 0 0.09 0.1 – 0.18 0.09 0.14 0.08 0.13 0.15 – – – 0.19 0.35 0.28 0.29 0.3 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 3 2 0 1 1 – 0 0 0 0 1 3 – – – 2 0 0 0 0 1
Bud – 0 0 0 0 1 1 1 – 1 1 0 0 1 0 – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 1 3 2 3 0 1 – 0 0 0 2 6 7 – – – 0 0 0 0 0 0
Cytoplasm – 74 30 79 108 115 145 185 – 92 113 111 46 60 109 – – – 0 1 2 2 1 3
Endoplasmic Reticulum – 69 30 61 77 60 64 35 – 19 19 19 83 94 88 – – – 53 114 76 76 129 119
Endosome – 0 0 0 0 0 1 0 – 0 0 0 0 0 1 – – – 0 0 0 0 1 2
Golgi – 0 1 0 0 0 0 1 – 1 0 1 1 3 3 – – – 1 0 1 0 0 4
Mitochondria – 0 0 0 0 0 2 0 – 0 1 0 0 0 0 – – – 1 1 0 1 0 1
Nucleus – 0 0 0 0 0 2 0 – 0 0 0 0 1 0 – – – 3 0 0 0 0 0
Nuclear Periphery – 0 0 1 0 0 1 0 – 1 1 0 1 0 0 – – – 1 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 19 13 8 5 7 20 24 – 23 13 20 10 21 33 – – – 14 64 32 33 60 70
Unique Cell Count 149 66 140 183 178 222 242 128 138 142 133 162 225 80 183 118 115 200 208
Labelled Cell Count 162 75 155 194 186 237 248 137 148 151 143 187 244 80 183 118 115 200 208


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 25.1 19.0 19.9 19.1 17.9 17.6 17.6 – 15.6 17.5 16.5 23.2 23.8 21.7 – – – 24.3 25.6 25.7
Std Deviation (1e-4) – 4.4 4.0 4.1 3.9 3.9 4.5 4.3 – 4.4 4.4 4.5 5.3 5.6 4.5 – – – 5.9 5.4 5.8
Intensity Change (Log2) – – – 0.07 0.01 -0.08 -0.11 -0.11 – -0.28 -0.12 -0.2 0.29 0.33 0.2 – – – 0.36 0.43 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cytoplasm 1.5 1.9 2.7 2.9 4.9 – 3.6 5.3 4.7 -1.5 -1.2 0.4 – – –
Endoplasmic Reticulum -0.3 -0.5 -1.7 -2.5 -5.5 – -4.6 -5.0 -5.1 2.3 1.7 -0.9 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole -3.1 0 -4.0 -2.4 -2.2 – -0.3 -2.1 -1.0 -2.5 -1.3 -1.0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.5054 22.1586 21.1476 18.4524 19.1968 20.7296 16.8148 23.1437 23.4973 24.4608 19.9697 22.796 15.396 23.2569 21.5715 21.1734 20.3531 21.5135
Actin 0.0547 0.0001 0.0087 0.003 0.0145 0.0003 0.2161 0.003 0.0002 0.0002 0.0052 0.0194 0.0441 0.0001 0.0037 0.0227 0.043 0.0006
Bud 0.0007 0.0001 0.0006 0.0009 0.0003 0.0001 0.0007 0.0003 0.0001 0.0003 0.0003 0.0002 0.0003 0 0.0002 0.0001 0.0009 0.0001
Bud Neck 0.0129 0.0006 0.0013 0.0018 0.0045 0.0066 0.0006 0.0008 0.0005 0.0016 0.0013 0.0014 0.0026 0.0003 0.0009 0.0008 0.0017 0.0008
Bud Periphery 0.0014 0.0005 0.0013 0.0024 0.0016 0.0008 0.0008 0.0011 0.0007 0.002 0.0004 0.0011 0.0006 0.0003 0.0008 0.0009 0.0031 0.0005
Bud Site 0.0046 0.0003 0.0091 0.0034 0.0006 0.0001 0.0034 0.0011 0.0001 0.0003 0.0016 0.0003 0.0041 0.0001 0.0009 0.0004 0.0011 0.0001
Cell Periphery 0.0129 0.0667 0.075 0.0705 0.092 0.0444 0.0024 0.0734 0.0319 0.042 0.0039 0.0437 0.0139 0.0832 0.071 0.0726 0.1138 0.0518
Cytoplasm 0.0434 0.0526 0.0187 0.0273 0.0886 0.0252 0.03 0.0289 0.0126 0.0244 0.0172 0.0058 0.0166 0.0102 0.0147 0.0056 0.0062 0.0077
Cytoplasmic Foci 0.0293 0.0011 0.0053 0.0135 0.0057 0.001 0.0098 0.0018 0.0007 0.0008 0.0009 0.0198 0.0179 0.0002 0.0096 0.003 0.0017 0.0004
Eisosomes 0.0005 0 0 0.0001 0.0001 0 0.0021 0 0 0 0.0001 0.0001 0.0003 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.4807 0.5645 0.5705 0.4757 0.3648 0.6337 0.5661 0.6073 0.7356 0.5904 0.7647 0.6583 0.6022 0.6634 0.5577 0.6412 0.5459 0.7042
Endosome 0.0635 0.0074 0.0144 0.0202 0.0191 0.0106 0.0534 0.013 0.0042 0.0182 0.0083 0.0069 0.0339 0.0028 0.014 0.0053 0.0064 0.008
Golgi 0.0131 0.0005 0.0109 0.0058 0.0069 0.0008 0.0286 0.0034 0.0005 0.0014 0.0008 0.0096 0.0083 0.0001 0.0036 0.0016 0.0089 0.0004
Lipid Particles 0.0169 0.0245 0.019 0.0122 0.0205 0.0136 0.0041 0.0357 0.0769 0.0103 0.0023 0.0092 0.0265 0.0099 0.0157 0.0127 0.0134 0.01
Mitochondria 0.0071 0.0002 0.0012 0.0078 0.0531 0.0005 0.004 0.0003 0.0001 0.0005 0.001 0.0021 0.0127 0 0.0007 0.0004 0.0029 0.0002
None 0.0127 0.0001 0.0001 0.0002 0.0008 0.0004 0.0013 0.0001 0.0001 0.0001 0.0011 0.0002 0.0045 0 0.0016 0.0001 0.0001 0.0001
Nuclear Periphery 0.0865 0.0324 0.0348 0.0283 0.0409 0.0762 0.0425 0.0177 0.0243 0.033 0.0372 0.0301 0.0995 0.0403 0.0573 0.0517 0.0287 0.0585
Nucleolus 0.0022 0.0004 0.0005 0.0003 0.0008 0.0008 0.0001 0.0004 0.0001 0.0002 0.003 0.0002 0.0003 0.0001 0.0003 0.0001 0.0003 0.0006
Nucleus 0.012 0.003 0.0023 0.0023 0.0079 0.0061 0.0041 0.002 0.0035 0.0022 0.0302 0.0013 0.0066 0.0014 0.0043 0.0015 0.0017 0.004
Peroxisomes 0.006 0 0.0019 0.0032 0.0034 0 0.0032 0.0001 0 0 0.0001 0.0008 0.0103 0 0.0037 0.0113 0.001 0
Punctate Nuclear 0.0197 0.0002 0.0011 0.0003 0.0059 0.0002 0.0011 0.0002 0.0002 0.0001 0.0159 0.0012 0.0033 0 0.0031 0.0001 0.0003 0.0005
Vacuole 0.1088 0.2401 0.2179 0.2974 0.2638 0.1671 0.021 0.205 0.1054 0.2592 0.1019 0.1801 0.0837 0.1846 0.2273 0.1592 0.2148 0.1464
Vacuole Periphery 0.0106 0.0047 0.0053 0.0233 0.0044 0.0116 0.0046 0.0046 0.0023 0.0129 0.0026 0.0083 0.0077 0.0029 0.0089 0.0085 0.004 0.0048

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 251.7893 221.2433 223.4564 206.6776 200.5531 223.3071 214.378 197.1149 219.1936 205.2273
Translational Efficiency 1.0338 1.1177 1.0844 1.0795 1.14 1.1906 1.1638 1.2517 1.0411 1.0068

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
43 180 2468 469 92 1555 551 31 135 1735 3019 500

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1398.19 1987.77 3227.47 3085.12 1411.46 1782.66 3422.34 3394.86 1407.23 1803.94 3263.04 3104.32
Standard Deviation 247.75 403.22 545.17 656.07 361.62 354.04 536.94 503.91 329.70 364.86 548.86 651.97
Intensity Change Log 2 — 0.507590 1.206843 1.141766 — 0.336843 1.277795 1.266164 — 0.424339 1.242923 1.205599

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000169 0.000139 0.000080 0.001334 0.000938 0.000194 0.000320 0.000070 0.000693 0.000188 0.000124 0.001255
Bud Neck 0.000876 0.000584 0.000620 0.009124 0.007965 0.000354 0.003259 0.001088 0.005707 0.000378 0.001102 0.008626
Bud Site 0.002463 0.002891 0.000454 0.005933 0.022251 0.001147 0.002861 0.000232 0.015948 0.001328 0.000893 0.005580
Cell Periphery 0.031591 0.054459 0.070492 0.125779* 0.029627 0.027623 0.127323* 0.157314* 0.030252 0.030407 0.080864 0.127734*
Cytoplasm 0.002309 0.000623 0.000698 0.000506 0.018394 0.001686 0.000482 0.000125 0.013270 0.001576 0.000658 0.000482
Cytoplasmic Foci 0.000505 0.000833 0.000035 0.002932 0.022149 0.001122 0.000181 0.000036 0.015255 0.001092 0.000062 0.002753
Eisosomes 0.000022 0.000044 0.000195 0.000432 0.000120 0.000024 0.001083 0.000658 0.000089 0.000026 0.000357 0.000446
Endoplasmic Reticulum 0.247551* 0.717987* 0.800407* 0.420172* 0.419059* 0.829140* 0.584546* 0.593293* 0.364431* 0.817608* 0.761010* 0.430906*
Endosome 0.004972 0.008405 0.002412 0.014035 0.040527 0.012129 0.007372 0.006645 0.029202 0.011743 0.003317 0.013577
Golgi 0.000373 0.001563 0.000992 0.007308 0.012199 0.001794 0.003655 0.010747 0.008433 0.001770 0.001478 0.007521
Lipid Particles 0.000526 0.001545 0.002945 0.038700 0.024241 0.002307 0.024328 0.023251 0.016687 0.002228 0.006847 0.037742
Mitochondria 0.000100 0.000249 0.000162 0.001275 0.000775 0.000311 0.000276 0.000086 0.000560 0.000304 0.000183 0.001202
Mitotic Spindle 0.000011 0.000304 0.000040 0.001267 0.000407 0.000044 0.000198 0.000032 0.000281 0.000071 0.000069 0.001190
None 0.000006 0.000009 0.000080 0.000095 0.000185 0.000248 0.000039 0.000047 0.000128 0.000223 0.000072 0.000092
Nuclear Periphery 0.000192 0.000761 0.002074 0.002969 0.004450 0.000537 0.004728 0.003772 0.003093 0.000560 0.002558 0.003019
Nuclear Periphery Foci 0.000471 0.002604 0.001009 0.007570 0.028756 0.002591 0.004362 0.002235 0.019747 0.002592 0.001621 0.007239
Nucleolus 0.000135 0.000047 0.000017 0.000098 0.000175 0.000074 0.000028 0.000017 0.000162 0.000071 0.000019 0.000093
Nucleus 0.000447 0.000079 0.000064 0.000160 0.001915 0.000128 0.000099 0.000037 0.001447 0.000123 0.000070 0.000153
Peroxisomes 0.000079 0.000033 0.000124 0.000249 0.000755 0.000108 0.000289 0.000061 0.000540 0.000100 0.000154 0.000237
Vacuole 0.680067* 0.199387 0.115980 0.354058* 0.349202* 0.114396 0.231276* 0.197726 0.454589* 0.123214 0.137023 0.344365*
Vacuole Periphery 0.027135 0.007453 0.001121 0.006002 0.015911 0.004042 0.003294 0.002529 0.019486 0.004396 0.001518 0.005787

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.23 0.49 -1.61 -1.65 -1.68 2.75 2.19 3.51 1.46 2.18 2.54 3.02 -0.82 -1.55 -1.62
Bud Neck 0.79 0.51 -5.89 -6.14 -6.09 2.15 1.05 1.95 -2.49 3.31 2.20 1.80 -1.30 -6.32 -5.62
Bud Site -0.17 1.68 -2.13 -1.39 -3.32 2.96 2.64 3.10 3.26 2.33 2.96 3.01 1.80 -2.88 -3.02
Cell Periphery -2.17 -5.61 -9.48 -6.27 -6.94 0.27 -10.09 -4.11 -4.28 -1.02 -0.03 -8.87 -10.93 -12.99 -6.06
Cytoplasm 2.11 1.92 1.95 -0.34 0.32 3.13 3.35 3.43 4.93 2.70 3.16 3.38 3.40 2.31 0.30
Cytoplasmic Foci -0.48 1.26 -1.81 -1.50 -2.22 3.22 3.36 3.39 4.68 1.91 3.12 3.35 2.64 -1.36 -2.19
Eisosomes -1.59 -7.13 -7.41 -7.10 -4.03 3.24 -1.84 -2.95 -3.51 0.75 3.00 -2.71 -6.37 -8.06 -0.80
Endoplasmic Reticulum -9.60 -12.81 -3.61 10.28 21.28 -10.06 -3.73 -2.49 4.04 -0.25 -14.19 -12.36 -1.71 22.19 19.05
Endosome -1.78 1.69 -4.42 -2.81 -7.51 3.93 4.57 4.62 3.03 0.55 3.37 5.04 2.92 -1.15 -7.00
Golgi -1.94 -2.56 -4.41 -3.46 -4.03 3.19 2.61 0.23 -1.71 -1.35 2.92 3.07 0.38 -3.75 -4.01
Lipid Particles -1.30 -5.37 -7.33 -7.07 -6.81 3.01 -0.13 0.08 -2.76 0.23 2.86 1.86 -3.05 -7.23 -6.18
Mitochondria -0.75 -1.99 -1.31 -1.12 -1.22 1.73 1.84 2.74 2.22 2.67 1.31 2.07 -0.75 -1.06 -1.19
Mitotic Spindle -0.98 -1.68 -1.63 -1.22 -1.57 1.49 0.74 1.55 0.58 1.36 1.24 1.15 -1.27 -1.56 -1.54
None -0.52 -2.16 -1.56 -1.51 -0.20 -0.37 1.53 1.40 1.40 -0.10 -0.67 0.78 0.45 0.97 -0.29
Nuclear Periphery -6.47 -17.88 -12.10 -9.18 -3.69 1.53 -0.12 0.24 -3.77 1.06 1.45 0.29 -0.00 -10.95 -2.04
Nuclear Periphery Foci -3.91 -4.35 -4.29 -3.04 -3.94 2.07 1.88 2.09 0.46 2.46 1.97 2.07 1.30 -3.07 -3.57
Nucleolus 1.78 2.75 0.33 -1.46 -2.46 1.83 3.12 3.42 1.95 2.15 2.09 4.13 1.03 -0.84 -2.40
Nucleus 1.25 1.27 0.88 -1.91 -2.79 2.40 2.40 2.52 2.84 2.66 2.55 2.63 2.44 -1.23 -2.49
Peroxisomes 1.24 -1.19 -1.70 -2.28 -1.33 2.95 1.58 3.10 0.99 1.08 2.88 2.47 1.70 -1.54 -0.89
Vacuole 10.29 13.37 7.26 -6.31 -16.05 6.43 3.01 2.87 -2.12 0.95 10.57 10.09 3.12 -15.30 -14.52
Vacuole Periphery 1.63 2.18 1.76 0.75 -5.80 2.90 3.07 3.24 1.76 0.92 3.20 3.81 2.87 -1.61 -5.37
Standard name
Human Ortholog
Description Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
Localization
Cell Percentages ER (46%), vacuole (10%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vma16

Vma16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available