Standard name | |
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Human Ortholog | |
Description | Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 3 | 2 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 1 | 3 | – | – | – | 2 | 0 | 0 | 0 | 0 | 1 |
Bud | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 1 | 1 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | 0 | 1 | 3 | 2 | 3 | 0 | 1 | – | 0 | 0 | 0 | 2 | 6 | 7 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 74 | 30 | 79 | 108 | 115 | 145 | 185 | – | 92 | 113 | 111 | 46 | 60 | 109 | – | – | – | 0 | 1 | 2 | 2 | 1 | 3 |
Endoplasmic Reticulum | – | 69 | 30 | 61 | 77 | 60 | 64 | 35 | – | 19 | 19 | 19 | 83 | 94 | 88 | – | – | – | 53 | 114 | 76 | 76 | 129 | 119 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 1 | 2 |
Golgi | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 1 | 3 | 3 | – | – | – | 1 | 0 | 1 | 0 | 0 | 4 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 1 | 1 | 0 | 1 | 0 | 1 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 1 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 19 | 13 | 8 | 5 | 7 | 20 | 24 | – | 23 | 13 | 20 | 10 | 21 | 33 | – | – | – | 14 | 64 | 32 | 33 | 60 | 70 |
Unique Cell Count | 149 | 66 | 140 | 183 | 178 | 222 | 242 | 128 | 138 | 142 | 133 | 162 | 225 | 80 | 183 | 118 | 115 | 200 | 208 | |||||
Labelled Cell Count | 162 | 75 | 155 | 194 | 186 | 237 | 248 | 137 | 148 | 151 | 143 | 187 | 244 | 80 | 183 | 118 | 115 | 200 | 208 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 25.1 | 19.0 | 19.9 | 19.1 | 17.9 | 17.6 | 17.6 | – | 15.6 | 17.5 | 16.5 | 23.2 | 23.8 | 21.7 | – | – | – | 24.3 | 25.6 | 25.7 |
Std Deviation (1e-4) | – | 4.4 | 4.0 | 4.1 | 3.9 | 3.9 | 4.5 | 4.3 | – | 4.4 | 4.4 | 4.5 | 5.3 | 5.6 | 4.5 | – | – | – | 5.9 | 5.4 | 5.8 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.01 | -0.08 | -0.11 | -0.11 | – | -0.28 | -0.12 | -0.2 | 0.29 | 0.33 | 0.2 | – | – | – | 0.36 | 0.43 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 1.5 | 1.9 | 2.7 | 2.9 | 4.9 | – | 3.6 | 5.3 | 4.7 | -1.5 | -1.2 | 0.4 | – | – | – |
Endoplasmic Reticulum | -0.3 | -0.5 | -1.7 | -2.5 | -5.5 | – | -4.6 | -5.0 | -5.1 | 2.3 | 1.7 | -0.9 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | -3.1 | 0 | -4.0 | -2.4 | -2.2 | – | -0.3 | -2.1 | -1.0 | -2.5 | -1.3 | -1.0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.5054 | 22.1586 | 21.1476 | 18.4524 | 19.1968 | 20.7296 | 16.8148 | 23.1437 | 23.4973 | 24.4608 | 19.9697 | 22.796 | 15.396 | 23.2569 | 21.5715 | 21.1734 | 20.3531 | 21.5135 |
Actin | 0.0547 | 0.0001 | 0.0087 | 0.003 | 0.0145 | 0.0003 | 0.2161 | 0.003 | 0.0002 | 0.0002 | 0.0052 | 0.0194 | 0.0441 | 0.0001 | 0.0037 | 0.0227 | 0.043 | 0.0006 |
Bud | 0.0007 | 0.0001 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0009 | 0.0001 |
Bud Neck | 0.0129 | 0.0006 | 0.0013 | 0.0018 | 0.0045 | 0.0066 | 0.0006 | 0.0008 | 0.0005 | 0.0016 | 0.0013 | 0.0014 | 0.0026 | 0.0003 | 0.0009 | 0.0008 | 0.0017 | 0.0008 |
Bud Periphery | 0.0014 | 0.0005 | 0.0013 | 0.0024 | 0.0016 | 0.0008 | 0.0008 | 0.0011 | 0.0007 | 0.002 | 0.0004 | 0.0011 | 0.0006 | 0.0003 | 0.0008 | 0.0009 | 0.0031 | 0.0005 |
Bud Site | 0.0046 | 0.0003 | 0.0091 | 0.0034 | 0.0006 | 0.0001 | 0.0034 | 0.0011 | 0.0001 | 0.0003 | 0.0016 | 0.0003 | 0.0041 | 0.0001 | 0.0009 | 0.0004 | 0.0011 | 0.0001 |
Cell Periphery | 0.0129 | 0.0667 | 0.075 | 0.0705 | 0.092 | 0.0444 | 0.0024 | 0.0734 | 0.0319 | 0.042 | 0.0039 | 0.0437 | 0.0139 | 0.0832 | 0.071 | 0.0726 | 0.1138 | 0.0518 |
Cytoplasm | 0.0434 | 0.0526 | 0.0187 | 0.0273 | 0.0886 | 0.0252 | 0.03 | 0.0289 | 0.0126 | 0.0244 | 0.0172 | 0.0058 | 0.0166 | 0.0102 | 0.0147 | 0.0056 | 0.0062 | 0.0077 |
Cytoplasmic Foci | 0.0293 | 0.0011 | 0.0053 | 0.0135 | 0.0057 | 0.001 | 0.0098 | 0.0018 | 0.0007 | 0.0008 | 0.0009 | 0.0198 | 0.0179 | 0.0002 | 0.0096 | 0.003 | 0.0017 | 0.0004 |
Eisosomes | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0021 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.4807 | 0.5645 | 0.5705 | 0.4757 | 0.3648 | 0.6337 | 0.5661 | 0.6073 | 0.7356 | 0.5904 | 0.7647 | 0.6583 | 0.6022 | 0.6634 | 0.5577 | 0.6412 | 0.5459 | 0.7042 |
Endosome | 0.0635 | 0.0074 | 0.0144 | 0.0202 | 0.0191 | 0.0106 | 0.0534 | 0.013 | 0.0042 | 0.0182 | 0.0083 | 0.0069 | 0.0339 | 0.0028 | 0.014 | 0.0053 | 0.0064 | 0.008 |
Golgi | 0.0131 | 0.0005 | 0.0109 | 0.0058 | 0.0069 | 0.0008 | 0.0286 | 0.0034 | 0.0005 | 0.0014 | 0.0008 | 0.0096 | 0.0083 | 0.0001 | 0.0036 | 0.0016 | 0.0089 | 0.0004 |
Lipid Particles | 0.0169 | 0.0245 | 0.019 | 0.0122 | 0.0205 | 0.0136 | 0.0041 | 0.0357 | 0.0769 | 0.0103 | 0.0023 | 0.0092 | 0.0265 | 0.0099 | 0.0157 | 0.0127 | 0.0134 | 0.01 |
Mitochondria | 0.0071 | 0.0002 | 0.0012 | 0.0078 | 0.0531 | 0.0005 | 0.004 | 0.0003 | 0.0001 | 0.0005 | 0.001 | 0.0021 | 0.0127 | 0 | 0.0007 | 0.0004 | 0.0029 | 0.0002 |
None | 0.0127 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0004 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.0045 | 0 | 0.0016 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0865 | 0.0324 | 0.0348 | 0.0283 | 0.0409 | 0.0762 | 0.0425 | 0.0177 | 0.0243 | 0.033 | 0.0372 | 0.0301 | 0.0995 | 0.0403 | 0.0573 | 0.0517 | 0.0287 | 0.0585 |
Nucleolus | 0.0022 | 0.0004 | 0.0005 | 0.0003 | 0.0008 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0006 |
Nucleus | 0.012 | 0.003 | 0.0023 | 0.0023 | 0.0079 | 0.0061 | 0.0041 | 0.002 | 0.0035 | 0.0022 | 0.0302 | 0.0013 | 0.0066 | 0.0014 | 0.0043 | 0.0015 | 0.0017 | 0.004 |
Peroxisomes | 0.006 | 0 | 0.0019 | 0.0032 | 0.0034 | 0 | 0.0032 | 0.0001 | 0 | 0 | 0.0001 | 0.0008 | 0.0103 | 0 | 0.0037 | 0.0113 | 0.001 | 0 |
Punctate Nuclear | 0.0197 | 0.0002 | 0.0011 | 0.0003 | 0.0059 | 0.0002 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0159 | 0.0012 | 0.0033 | 0 | 0.0031 | 0.0001 | 0.0003 | 0.0005 |
Vacuole | 0.1088 | 0.2401 | 0.2179 | 0.2974 | 0.2638 | 0.1671 | 0.021 | 0.205 | 0.1054 | 0.2592 | 0.1019 | 0.1801 | 0.0837 | 0.1846 | 0.2273 | 0.1592 | 0.2148 | 0.1464 |
Vacuole Periphery | 0.0106 | 0.0047 | 0.0053 | 0.0233 | 0.0044 | 0.0116 | 0.0046 | 0.0046 | 0.0023 | 0.0129 | 0.0026 | 0.0083 | 0.0077 | 0.0029 | 0.0089 | 0.0085 | 0.004 | 0.0048 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 251.7893 | 221.2433 | 223.4564 | 206.6776 | 200.5531 | 223.3071 | 214.378 | 197.1149 | 219.1936 | 205.2273 |
Translational Efficiency | 1.0338 | 1.1177 | 1.0844 | 1.0795 | 1.14 | 1.1906 | 1.1638 | 1.2517 | 1.0411 | 1.0068 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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