Standard name
Human Ortholog
Description Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.07 0.07 0 0 0 0 0 0 0.1 0.1 0.17 0.09 0.09 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.07 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.81 0.79 0.67 0.57 0.56 0.65 0.6 0.61 0.64 0.71 0.68 0.78 0.77 0.64 0.78 0.59 0.67 0.87 0.85 0.72 0.78 0.7 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0.05 0 0 0.09 0.14 0.18 0.13 0.13 0.16 0 0 0 0 0.07 0 0.07 0 0.15 0.06 0.11 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.15 0.12 0.24 0.38 0.36 0.23 0.25 0.17 0.18 0.12 0.15 0.14 0.18 0.2 0.15 0.26 0.2 0 0.06 0.07 0.1 0.12 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 1 0 1 0
Bud 0 0 0 0 0 1 0 0 1 2 0 1 0 0 0 0 0 0 5 1 2 1 7 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 2 1 3 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 6 12 20 21 14 16 15 12 9 4 13 13 30 7 8 7 1 0 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 3 4 0 0 0 0 0 0 0
Endosome 0 0 1 0 1 0 1 3 0 0 0 0 0 0 2 0 1 0 0 0 1 1 1 5
Golgi 0 0 1 2 6 2 2 3 1 0 0 0 0 0 2 0 0 0 0 0 2 3 0 2
Mitochondria 0 3 2 0 2 2 4 13 22 25 30 18 0 0 0 0 1 1 0 2 2 0 2 2
Nucleus 371 94 165 133 163 178 252 263 226 239 217 247 101 101 113 61 52 43 402 129 187 152 202 233
Nuclear Periphery 0 0 1 0 0 0 2 0 2 4 0 0 0 0 0 0 1 0 1 0 1 0 0 1
Nucleolus 5 6 9 9 11 14 35 60 66 49 40 58 1 1 3 1 6 1 30 6 39 12 32 32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 3
Vac/Vac Membrane 42 17 26 48 109 114 89 111 61 68 38 54 18 24 35 12 23 13 15 8 17 19 35 81
Unique Cell Count 411 116 208 199 287 319 385 440 369 373 307 363 130 132 177 78 88 64 462 152 261 195 290 379
Labelled Cell Count 421 124 211 204 312 333 399 471 395 402 337 385 134 139 186 84 98 66 462 152 261 195 290 379


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.2 15.3 19.5 21.8 22.2 21.4 20.0 18.4 17.6 15.9 16.0 16.5 19.4 19.4 19.5 14.2 16.3 18.9 21.0 21.7 23.3
Std Deviation (1e-4) 3.8 4.3 4.5 4.6 5.1 4.5 4.4 4.6 4.6 4.0 4.5 4.4 4.9 4.9 6.1 5.8 6.2 6.2 4.8 5.4 6.8
Intensity Change (Log2) – – – 0.16 0.19 0.13 0.03 -0.08 -0.15 -0.3 -0.28 -0.24 -0.01 -0.01 -0.0 -0.46 -0.26 -0.05 0.11 0.16 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 2.0 1.9 0.5 0.5 0.7 0.2 0 0 2.8 2.7 4.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.9 3.2 4.1 2.5 0 0 0 0 0 0
Nucleus -2.8 -5.2 -5.5 -3.5 -4.9 -4.5 -3.8 -2.2 -2.9 -0.4 -0.6 -3.4 -0.2 -3.6 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 -0.3 0 2.1 3.6 4.7 3.4 3.3 4.2 0 0 -1.5 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 6.3 5.9 3.1 3.7 1.3 1.8 0 0.8 0.4 1.4 1.9 0.6 2.9 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0352 0 0.0012 0.0007 0.0001 0.0119 0.0007 0 0.0044 0.0005 0.0142 0.0001 – – – – – –
Bud 0.0006 0 0.0001 0.0004 0.0002 0.0001 0.0006 0 0.0001 0.0001 0.0002 0.0001 – – – – – –
Bud Neck 0.0009 0 0.0002 0.0003 0.0003 0.0007 0.0005 0 0 0.0002 0.0005 0.0006 – – – – – –
Bud Periphery 0.0007 0 0.0001 0.0005 0.0003 0.0001 0.0003 0 0.0001 0.0001 0.0003 0.0001 – – – – – –
Bud Site 0.002 0 0.0016 0.0009 0.0002 0.0001 0.0042 0 0.0002 0 0.0012 0 – – – – – –
Cell Periphery 0.0005 0 0.0001 0.0009 0.0012 0.0001 0.0002 0 0 0 0.0001 0.0001 – – – – – –
Cytoplasm 0.0112 0.0005 0.0019 0.0042 0.002 0.0014 0.0041 0.0005 0.0009 0.005 0.0039 0.0002 – – – – – –
Cytoplasmic Foci 0.0196 0 0.0098 0.0168 0.0055 0.0024 0.0151 0 0.0013 0 0.0029 0 – – – – – –
Eisosomes 0.0003 0 0.0001 0.0001 0 0 0 0 0 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0071 0 0.0004 0.0003 0.0007 0.001 0.0002 0 0.0006 0.0002 0.0047 0 – – – – – –
Endosome 0.0216 0 0.002 0.0028 0.0016 0.0052 0.0025 0 0.0061 0.0001 0.0238 0 – – – – – –
Golgi 0.0099 0 0.0017 0.003 0.0002 0.0019 0.0011 0 0.0029 0 0.0042 0 – – – – – –
Lipid Particles 0.018 0 0.0085 0.0551 0.01 0.0042 0.001 0 0.0003 0 0.0032 0 – – – – – –
Mitochondria 0.0051 0.0003 0.0039 0.0026 0.0083 0.0014 0.0024 0.0002 0.0029 0.0007 0.0029 0.0005 – – – – – –
None 0.0178 0 0.0018 0.0034 0.0009 0.0002 0.0011 0 0.0002 0 0.0055 0 – – – – – –
Nuclear Periphery 0.0262 0.0097 0.0131 0.0211 0.0369 0.027 0.0196 0.0109 0.0083 0.0327 0.0314 0.0084 – – – – – –
Nucleolus 0.0406 0.007 0.0083 0.0207 0.0482 0.0109 0.0244 0.0054 0.0055 0.0014 0.0253 0.0252 – – – – – –
Nucleus 0.7483 0.9819 0.9396 0.8397 0.8548 0.9235 0.8341 0.9827 0.9652 0.9578 0.8515 0.9634 – – – – – –
Peroxisomes 0.0049 0 0.0031 0.0217 0.0003 0.0036 0.001 0 0.0003 0 0.0009 0 – – – – – –
Punctate Nuclear 0.0196 0.0001 0.0012 0.0023 0.004 0.0007 0.0843 0.0001 0.0003 0.0003 0.0208 0.0002 – – – – – –
Vacuole 0.0051 0.0001 0.0005 0.0017 0.0075 0.0018 0.001 0 0.0001 0.0003 0.0008 0.0006 – – – – – –
Vacuole Periphery 0.0048 0.0002 0.0009 0.0007 0.0166 0.0018 0.0015 0.0001 0.0003 0.0005 0.0015 0.0004 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 180.0155 145.8725 175.2517 187.8738 155.4387 159.0212 165.2173 177.5365 198.0119 164.5973
Translational Efficiency 1.4824 1.5551 1.2082 1.2662 1.2025 1.6003 1.3256 0.9939 0.9949 1.1584

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1462 469 161 242 1153 531 212 1546 2615 1000 373 1788

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1927.43 1768.59 1649.56 2292.70 1267.49 1697.23 2129.48 1366.00 1636.45 1730.70 1922.33 1491.43
Standard Deviation 346.32 390.46 252.01 511.98 275.58 317.06 270.55 373.73 455.96 355.18 354.29 506.70
Intensity Change Log 2 — -0.124079 -0.224597 0.250369 — 0.421208 0.748527 0.107983 — 0.117417 0.242240 0.195551

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001372 0.000807 0.001842 0.001567 0.000868 0.001014 0.001611 0.000991 0.001150 0.000917 0.001711 0.001069
Bud Neck 0.025575 0.016976 0.018700 0.051651 0.014771 0.014625 0.035746 0.020885 0.020811 0.015728 0.028388 0.025049
Bud Site 0.003645 0.001083 0.001725 0.002986 0.001194 0.000839 0.001526 0.002446 0.002564 0.000954 0.001612 0.002519
Cell Periphery 0.000161 0.000109 0.000126 0.000292 0.000190 0.000064 0.000115 0.000231 0.000173 0.000085 0.000120 0.000240
Cytoplasm 0.001666 0.003721 0.001452 0.025845 0.001910 0.000576 0.000706 0.040862 0.001774 0.002051 0.001028 0.038829
Cytoplasmic Foci 0.001557 0.000454 0.000181 0.000868 0.000412 0.000220 0.000173 0.000619 0.001052 0.000330 0.000176 0.000653
Eisosomes 0.000034 0.000024 0.000035 0.000026 0.000035 0.000026 0.000043 0.000009 0.000034 0.000025 0.000040 0.000011
Endoplasmic Reticulum 0.001185 0.001845 0.003541 0.003655 0.004077 0.002761 0.002124 0.002899 0.002460 0.002331 0.002736 0.003002
Endosome 0.001255 0.000395 0.000262 0.000883 0.000838 0.000443 0.000182 0.000836 0.001071 0.000420 0.000216 0.000843
Golgi 0.001040 0.000200 0.000165 0.000340 0.000109 0.000225 0.000199 0.000591 0.000630 0.000213 0.000184 0.000557
Lipid Particles 0.000560 0.000165 0.000210 0.000732 0.000066 0.000194 0.000108 0.000212 0.000342 0.000181 0.000152 0.000283
Mitochondria 0.004045 0.001728 0.003015 0.009167 0.001550 0.002032 0.006220 0.003711 0.002945 0.001889 0.004836 0.004449
Mitotic Spindle 0.007705 0.006088 0.000658 0.047530 0.000897 0.002780 0.001178 0.005979 0.004703 0.004331 0.000954 0.011603
None 0.001253 0.002229 0.001054 0.005681 0.003667 0.000955 0.001713 0.014256 0.002317 0.001553 0.001429 0.013096
Nuclear Periphery 0.001191 0.000680 0.001012 0.040925 0.000824 0.000751 0.010857 0.008287 0.001029 0.000718 0.006607 0.012704
Nuclear Periphery Foci 0.000286 0.003042 0.000761 0.013317 0.000243 0.001737 0.000692 0.003602 0.000267 0.002349 0.000722 0.004917
Nucleolus 0.018994 0.017952 0.024492 0.011954 0.037558 0.029055 0.038381 0.007811 0.027179 0.023848 0.032386 0.008372
Nucleus 0.925757* 0.940997* 0.937124* 0.766541* 0.929122* 0.940159* 0.894473* 0.881573* 0.927241* 0.940552* 0.912882* 0.866004*
Peroxisomes 0.000561 0.000428 0.000912 0.000626 0.000459 0.000512 0.001397 0.000152 0.000516 0.000473 0.001188 0.000216
Vacuole 0.001327 0.000844 0.002433 0.014642 0.000864 0.000668 0.002137 0.003043 0.001123 0.000751 0.002265 0.004613
Vacuole Periphery 0.000831 0.000231 0.000299 0.000771 0.000348 0.000364 0.000417 0.001003 0.000618 0.000302 0.000366 0.000971

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.24 -2.87 -0.96 -5.09 2.58 -1.65 -8.67 -3.93 -0.53 6.07 1.25 -5.84 -0.43 -2.52 7.29
Bud Neck 5.81 3.75 -6.09 -8.25 -7.50 -0.52 -8.69 -8.47 -7.27 4.49 5.29 -4.98 -5.64 -9.20 0.92
Bud Site 3.30 2.04 -2.92 -7.24 -5.82 1.33 -2.86 -5.78 -6.31 0.74 3.65 -0.79 -2.05 -7.74 -0.73
Cell Periphery 1.51 0.27 -7.17 -7.99 -7.19 4.30 0.47 -1.32 -9.03 -2.16 4.09 0.40 -4.65 -10.08 -5.21
Cytoplasm -1.39 0.94 -6.11 -5.03 -6.23 2.46 1.92 -14.24 -15.17 -15.04 -0.49 2.28 -15.49 -14.34 -15.99
Cytoplasmic Foci 1.37 2.08 -1.52 -2.56 -2.98 0.92 -0.06 -0.62 -2.61 -0.81 1.74 1.66 -0.15 -2.98 -2.96
Eisosomes 3.94 -0.80 -1.00 -3.94 -0.21 3.75 -4.63 11.81 9.06 10.43 5.41 -4.22 12.32 7.30 11.57
Endoplasmic Reticulum -2.92 -6.46 -7.52 -4.58 0.79 3.25 6.50 3.12 -1.45 -4.14 0.76 -2.05 -5.17 -4.09 -1.65
Endosome 1.21 1.63 -0.92 -4.08 -6.70 0.92 1.25 0.30 -1.52 -3.64 1.45 1.99 0.15 -2.44 -4.47
Golgi 1.21 1.19 0.28 -4.56 -7.18 -1.30 -4.76 -2.12 -1.39 -0.86 0.98 0.68 -0.23 -1.77 -1.39
Lipid Particles 0.76 0.39 -3.78 -5.98 -5.68 -1.15 -2.64 -3.40 -0.85 -0.74 0.39 -0.04 -1.52 -3.73 -3.59
Mitochondria 4.47 0.79 -6.59 -12.18 -8.38 -3.30 -12.10 -12.97 -8.70 5.22 3.39 -6.59 -6.17 -12.86 1.57
Mitotic Spindle 0.35 2.45 -5.32 -5.33 -5.75 -1.12 -1.81 -5.18 -3.54 -1.23 0.02 -0.22 -6.01 -5.50 -4.90
None -1.76 1.57 -7.56 -5.12 -7.87 3.34 1.86 -5.47 -8.80 -7.49 1.79 1.87 -7.72 -8.67 -8.69
Nuclear Periphery 2.72 -0.82 -6.33 -6.48 -6.20 0.30 -2.26 -8.49 -8.57 -0.80 2.42 -2.29 -10.23 -10.58 -3.56
Nuclear Periphery Foci -1.92 -1.06 -3.88 -1.45 -3.35 -1.48 -1.73 -4.83 -1.88 -4.26 -2.42 -1.70 -5.76 -1.69 -4.85
Nucleolus 0.34 -1.22 4.97 3.63 3.37 2.16 -0.96 9.82 6.14 5.52 1.40 -1.97 10.33 6.88 6.36
Nucleus -2.21 -0.72 11.05 11.55 10.73 -1.71 5.22 10.39 10.50 0.18 -2.97 3.93 13.37 14.18 5.24
Peroxisomes 1.14 -2.92 0.53 -0.65 3.22 -0.64 -4.80 4.04 4.35 6.21 0.37 -5.49 5.79 4.48 7.72
Vacuole 2.31 -1.78 -7.50 -7.84 -7.03 0.54 -5.07 -6.12 -6.96 -0.53 2.44 -4.01 -8.15 -9.77 -5.03
Vacuole Periphery 1.75 1.30 0.55 -3.56 -2.17 -0.18 -1.76 -1.74 -1.64 -1.36 1.53 0.80 -1.04 -2.00 -1.60
Standard name
Human Ortholog
Description Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle
Localization
Cell Percentages nucleus (41%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpn1

Rpn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available