Standard name
Human Ortholog
Description Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of human homolog ALDH4A1 causes type II hyperprolinemia (HPII), an autosomal recessive inborn error of metabolism; human homolog ALDH4A1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.11 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.12 0.28 0.23 0.33 0.09 0.08 0.11 0 0.09 0.06 0.11 0.37 0.42 0.53 0.52 0.5 0.4 0.05 0.12 0.11 0.11 0.16 0.13
Mitochondria 0.96 0.84 0.83 0.93 0.76 0.8 0.8 0.84 0.9 0.87 0.93 0.86 0.85 0.82 0.67 0.17 0.18 0.09 0.9 0.75 0.71 0.72 0.68 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0.08 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0.08 0.08 0.16 0.14 0.11 0.09 0.13 0.12 0.1 0 0 0 0.1 0.09 0.14 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0 0 0.08 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0.13 0 0.06 0.07 0.05 0 0 0.05 0 0.07 0.06 0.07 0.05 0.11 0.11 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 9 6 4 4 0 4 4 0 3 0 0 4 5 6 19 19 18 1 12 3 2 6 12
Bud 2 3 0 1 3 1 2 3 0 0 2 2 2 1 0 6 1 1 0 2 2 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 15 3 1 0 0 1 0 0 1 2 5 3 3 9 8 7 5 0 0 0 0 0 0
Cytoplasm 2 1 1 3 6 5 6 5 4 3 5 3 5 9 8 2 3 11 0 0 0 0 0 0
Endoplasmic Reticulum 0 2 0 0 0 2 1 1 2 1 1 3 0 2 5 5 8 7 0 1 0 0 0 0
Endosome 1 0 0 0 1 0 0 1 0 0 2 1 0 0 0 3 2 4 0 4 1 1 3 3
Golgi 5 46 26 26 66 16 16 30 2 17 14 20 75 101 135 100 92 64 9 53 12 11 30 25
Mitochondria 183 335 76 107 151 145 167 230 149 159 236 161 174 199 171 32 34 15 173 337 82 71 128 145
Nucleus 0 2 0 1 0 0 2 0 0 0 0 0 0 0 0 6 0 3 0 0 0 0 0 0
Nuclear Periphery 7 5 2 2 8 4 11 5 13 3 16 7 3 2 4 3 5 4 0 1 0 0 1 0
Nucleolus 8 18 1 9 16 29 30 30 15 24 30 18 6 4 6 19 16 23 0 5 0 2 3 2
Peroxisomes 3 10 3 1 2 2 4 5 1 0 2 1 2 1 3 10 14 13 4 11 8 2 4 5
SpindlePole 0 0 0 0 0 1 1 2 1 2 1 0 1 0 0 7 6 2 0 1 0 0 0 0
Vac/Vac Membrane 8 45 12 3 11 12 10 8 5 9 9 14 13 18 13 21 21 26 0 13 2 2 7 4
Unique Cell Count 190 397 92 115 198 182 209 273 165 183 254 187 205 242 255 192 184 161 192 449 116 99 190 202
Labelled Cell Count 224 491 131 158 268 217 255 324 192 222 320 235 288 345 360 241 229 196 192 449 116 99 190 202


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.0 24.8 20.8 21.5 25.4 25.4 23.0 24.2 21.3 21.6 21.2 21.5 22.3 22.3 22.0 29.3 30.4 28.7 18.8 21.5 23.0
Std Deviation (1e-4) 4.3 6.6 5.6 5.8 6.8 7.9 7.1 7.7 6.7 7.2 7.4 7.7 5.3 5.3 6.2 12.6 12.3 12.8 5.6 5.9 6.1
Intensity Change (Log2) – – – 0.05 0.29 0.29 0.15 0.22 0.03 0.05 0.03 0.05 0.1 0.1 0.08 0.49 0.55 0.46 -0.15 0.05 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.9 1.0 1.2
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.9 0.9 -4.2 -4.8 -4.0 -6.7 -4.1 -5.8 -3.7 1.4 2.3 4.1 3.8 3.4 1.8
Mitochondria 2.3 -1.2 -0.6 -0.5 0.4 1.8 0.9 2.8 0.8 0.5 -0.1 -2.8 -10.7 -10.3 -11.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1.9 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.4 3.7 3.5 2.9 2.5 3.3 3.1 2.7 0 0 0 2.7 2.5 3.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4 1.5
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -2.2 -1.8 -2.5 -3.7 -3.1 -2.4 -3.3 -1.5 -1.9 -1.6 -2.5 -0.5 -0.4 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.9216 15.9046 15.5074 14.9771 13.9787 14.896 13.3457 14.0949 13.5215 12.763 10.4607 12.9438 13.338 13.4495 13.1127 12.1078 11.9805 12.1076
Actin 0.0022 0.0026 0.0042 0.0006 0.0057 0.0011 0.0196 0.0014 0.0095 0.0171 0.0022 0.0051 0.0055 0.0114 0.0087 0.0011 0.0003 0.001
Bud 0.0001 0.0003 0.0001 0 0.0001 0 0.0008 0.0003 0.0006 0.0003 0.0001 0.0001 0.0002 0.0041 0.0001 0 0 0
Bud Neck 0.0001 0.0001 0 0 0.0001 0 0.0057 0.0002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0003 0 0 0 0
Bud Periphery 0.0002 0.0008 0.0002 0 0.0001 0.0001 0.0019 0.0005 0.0048 0.0004 0.0003 0.0002 0.0003 0.0019 0.0004 0.0001 0 0
Bud Site 0.0002 0.0007 0.0003 0 0.0002 0 0.0031 0.0018 0.0012 0.0004 0.0004 0.0001 0.0006 0.0037 0.0001 0.0001 0 0
Cell Periphery 0.0003 0.0008 0.0001 0 0.0002 0.0001 0.0003 0.0003 0.0003 0.0001 0.0001 0.0001 0.0002 0.0009 0.0005 0.0002 0.0001 0
Cytoplasm 0.0001 0 0.0001 0 0.0005 0 0.0026 0 0.0001 0.0004 0.0004 0.0001 0.0001 0.0002 0 0 0 0
Cytoplasmic Foci 0.0013 0.0011 0.0007 0.0001 0.0099 0.0001 0.006 0.0012 0.0012 0.0101 0.005 0.0009 0.0093 0.0043 0.001 0.0015 0.0001 0.0005
Eisosomes 0.0003 0.0007 0.0005 0.0002 0.0002 0.0002 0.0005 0.0003 0.0019 0.0001 0.0001 0.0004 0.0002 0.0003 0.0003 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0004 0.0001 0.0004 0 0.0024 0 0.0008 0.0002 0.0004 0.0005 0.0004 0.0003 0.0001 0.0002 0.0001 0 0 0
Endosome 0.0054 0.0044 0.0045 0.0004 0.0046 0.0012 0.0046 0.0028 0.0031 0.0156 0.0169 0.0044 0.0052 0.0049 0.0013 0.001 0.0003 0.0026
Golgi 0.0049 0.0247 0.0175 0.0008 0.0024 0.0081 0.004 0.01 0.0097 0.0136 0.0131 0.005 0.0188 0.0231 0.0084 0.0023 0.0013 0.0164
Lipid Particles 0.0137 0.0152 0.0057 0.0015 0.0089 0.0083 0.0137 0.006 0.0029 0.0072 0.0341 0.0196 0.0183 0.0071 0.0059 0.0181 0.0003 0.0084
Mitochondria 0.9405 0.9236 0.9573 0.995 0.9449 0.9672 0.9134 0.9603 0.947 0.9042 0.8933 0.9588 0.9148 0.8972 0.9634 0.9551 0.9959 0.9595
None 0.0001 0 0.0002 0 0.0007 0 0.0029 0 0.0003 0.0004 0.0007 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0043 0.0006 0.0022 0 0.0022 0 0.0011 0.0003 0.0009 0.0003 0.0061 0.0004 0.0001 0.0004 0 0 0 0.0001
Nucleolus 0.0004 0.0013 0.0002 0 0.0082 0.0082 0.0004 0.0003 0.0003 0.0001 0.0014 0.0001 0.0002 0.0033 0.0001 0.0001 0 0
Nucleus 0.0004 0.0003 0.0006 0 0.0013 0.0011 0.0007 0.0001 0.0005 0.0001 0.002 0.0001 0.0001 0.0006 0 0 0 0
Peroxisomes 0.0046 0.0028 0.0012 0.0006 0.0008 0.0006 0.0064 0.0064 0.0052 0.0171 0.0128 0.0015 0.0226 0.0072 0.0062 0.0171 0.0007 0.0087
Punctate Nuclear 0.0012 0 0.0002 0 0.0008 0 0.0027 0 0.0003 0.0012 0.0076 0.0002 0.0002 0 0 0 0 0
Vacuole 0.0033 0.0052 0.0008 0.0002 0.0016 0.0003 0.0035 0.0037 0.0027 0.0027 0.0012 0.0003 0.0007 0.0103 0.001 0.0008 0.0001 0.0003
Vacuole Periphery 0.0159 0.0146 0.0031 0.0006 0.0041 0.0033 0.0052 0.004 0.0072 0.008 0.0017 0.0021 0.0024 0.0186 0.0022 0.0021 0.0007 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.7643 46.8072 41.5101 51.4874 33.53 47.8643 54.2526 57.8468 64.9662 58.5267
Translational Efficiency 1.2134 1.048 1.2452 1.015 1.4025 1.6259 1.2561 1.1299 1.1723 1.0698

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of human homolog ALDH4A1 causes type II hyperprolinemia (HPII), an autosomal recessive inborn error of metabolism; human homolog ALDH4A1 can complement yeast null mutant
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Put2

Put2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Put2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available