Standard name
Human Ortholog
Description Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0.06 0 0.09 0.1
Bud 0 0 0 0.05 0 0 0 0.05 0.05 0.06 0.08 0.05 – 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0 – 0.11 0.12 – – 0 0 0 0 0 0.07 0.07
Cytoplasm 0.89 0.9 0.89 0.85 0.85 0.81 0.81 0.75 0.7 0.72 0.65 0.68 – 1.0 0.93 – – 0.96 0.34 0.39 0.07 0.18 0.24 0.15
Endoplasmic Reticulum 0.1 0.08 0 0 0 0.05 0 0 0 0 0 0 – 0 0 – – 0.06 0.34 0.28 0.58 0.31 0.3 0.32
Endosome 0 0 0 0 0.05 0 0 0.06 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.13 0.17 0.23 0.32 0.33 0.46 0.34 – 0 0 – – 0 0 0 0.09 0.23 0.14 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 0 0.19 0.14 0.1 0.11 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 4 3 2 3 0 2 2 1 1 6 – 0 0 – – 0 9 5 18 3 14 24
Bud 2 1 5 11 5 9 11 22 15 29 29 21 – 0 0 – – 2 3 0 2 1 0 3
Bud Neck 1 0 0 0 0 0 0 0 0 0 1 0 – 0 1 – – 4 13 10 6 5 4 10
Bud Site 0 0 0 0 0 1 0 0 1 2 1 1 – 0 0 – – 0 – – – – – –
Cell Periphery 5 4 11 13 16 19 14 8 14 13 11 16 – 4 15 – – 9 2 3 8 5 10 16
Cytoplasm 311 165 262 202 289 249 312 359 220 364 239 260 – 36 116 – – 422 139 77 21 21 36 36
Endoplasmic Reticulum 36 15 11 4 9 16 10 11 5 7 4 14 – 1 4 – – 27 135 56 175 36 45 75
Endosome 0 1 12 10 16 8 14 27 6 8 3 6 – 0 0 – – 3 2 3 3 0 4 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – 3 0 1 3 0 1 2
Mitochondria 0 4 2 11 11 40 66 112 102 165 171 131 – 0 0 – – 7 17 7 25 27 22 25
Nucleus 0 0 0 2 0 2 1 1 2 1 0 1 – 0 1 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 1 0 0 1 0 1 0 – 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 1 0 1 3 2 1 1 – 0 0 – – 1 0 0 0 0 0 0
Peroxisomes 0 0 2 6 16 1 0 2 2 4 1 2 – 0 0 – – 1 0 0 1 0 1 4
SpindlePole 0 0 3 1 4 0 2 1 1 5 1 2 – 0 0 – – 3 1 0 0 0 0 0
Vac/Vac Membrane 3 0 5 1 13 12 15 6 4 5 2 7 – 0 0 – – 4 75 27 31 12 11 23
Unique Cell Count 348 183 294 237 339 308 386 481 314 506 369 382 36 125 440 403 200 303 119 155 235
Labelled Cell Count 360 193 318 265 381 362 445 552 378 606 466 468 41 137 486 403 200 303 119 155 235


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.4 5.5 5.4 5.7 4.7 4.4 4.7 4.3 4.1 4.0 4.1 – 5.6 5.9 4.7 4.8 6.1 5.7 6.4 6.8
Std Deviation (1e-4) 0.6 0.8 1.8 1.4 2.2 1.4 0.9 1.6 1.2 1.1 1.3 0.8 – 0.9 0.5 1.3 1.3 1.9 1.6 1.5 1.5
Intensity Change (Log2) – – – -0.02 0.06 -0.23 -0.34 -0.23 -0.34 -0.43 -0.46 -0.43 – 0.03 0.09 -0.23 -0.19 0.15 0.05 0.22 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Bud 2.0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Cell Periphery 1.0 0.6 0 0 0 0 0 0 0 – 0 3.2 – – -1.4
Cytoplasm -1.3 -1.4 -2.8 -3.0 -4.9 -5.8 -5.7 -7.2 -6.5 – 2.1 1.2 – – 3.6
Endoplasmic Reticulum -1.4 -0.8 0 0 0 0 0 0 0 – 0 0 – – 1.4
Endosome 0.1 0.4 0 0 0 0 0 0 0 – 0 0 – – -3.2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Mitochondria 2.9 2.3 0 0 0 0 0 0 0 – 0 0 – – 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Peroxisomes 0 3.0 0 0 0 0 0 0 0 – 0 0 – – 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 – – 0
Vacuole 0 1.6 1.6 1.7 0 0 0 0 0.1 – 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0347 0.0145 0.0269 0.0136 0.0145 0.0096 0.0713 0.0032 0.0078 0.002 0.0126 0.0042 0.0026 0.017 0.0011 0.0022 0.0005 0.0003
Bud 0.0026 0.0027 0.0092 0.0134 0.0012 0.0012 0.0009 0.0007 0.0013 0.0001 0.0003 0.0003 0.0005 0.0065 0.0009 0.0005 0.0001 0
Bud Neck 0.0109 0.0006 0.0025 0.0024 0.0058 0.0028 0.0022 0.0008 0.0013 0.0014 0.0004 0.0053 0.0005 0.0011 0.0138 0.0003 0.0004 0.0004
Bud Periphery 0.0057 0.0073 0.0039 0.0404 0.0018 0.0011 0.0017 0.0015 0.0033 0.0003 0.0006 0.0008 0.0005 0.0107 0.0025 0.0023 0.0002 0
Bud Site 0.0132 0.0182 0.0137 0.0121 0.0036 0.0007 0.0051 0.0118 0.0071 0.0005 0.0006 0.0037 0.003 0.072 0.0079 0.0003 0.0002 0.0001
Cell Periphery 0.0024 0.0016 0.0018 0.0023 0.001 0.0004 0.0025 0.0025 0.0055 0.0017 0.0004 0.0009 0.0039 0.0084 0.0138 0.0043 0.0006 0.0006
Cytoplasm 0.2989 0.461 0.425 0.357 0.4038 0.4289 0.3929 0.5497 0.5264 0.5801 0.6714 0.5989 0.5106 0.5988 0.651 0.273 0.714 0.4946
Cytoplasmic Foci 0.0358 0.018 0.0181 0.0508 0.058 0.0285 0.0353 0.0185 0.01 0.0112 0.0291 0.0145 0.0031 0.051 0.0063 0.0062 0.0044 0.0101
Eisosomes 0.0008 0.0003 0.0006 0.0003 0.0002 0.0001 0.0012 0.0004 0.0004 0.0002 0.0001 0.0002 0.0001 0.0007 0.0002 0.0005 0 0
Endoplasmic Reticulum 0.0326 0.0276 0.0217 0.0132 0.0125 0.0126 0.0331 0.0307 0.0335 0.0151 0.0264 0.0299 0.0131 0.0572 0.1104 0.0061 0.006 0.0435
Endosome 0.0252 0.0058 0.0128 0.0322 0.0338 0.0136 0.0301 0.0121 0.0067 0.0054 0.0244 0.0036 0.0207 0.0141 0.0081 0.0021 0.0007 0.0052
Golgi 0.008 0.0009 0.0063 0.0113 0.0137 0.0041 0.0073 0.003 0.002 0.0064 0.0069 0.0007 0.0007 0.0018 0.0019 0.0005 0.0001 0.0003
Lipid Particles 0.0144 0.0025 0.0108 0.0132 0.0287 0.0114 0.0136 0.0091 0.0051 0.009 0.0265 0.0028 0.0014 0.0306 0.0042 0.0012 0.0002 0.0025
Mitochondria 0.0073 0.0008 0.0035 0.0086 0.0072 0.0046 0.0035 0.0021 0.0054 0.0017 0.0027 0.0005 0.0042 0.0003 0.0014 0.0012 0 0.0001
None 0.4426 0.4157 0.4248 0.3724 0.3792 0.4496 0.363 0.3285 0.3726 0.3544 0.1611 0.3266 0.0124 0.1192 0.1364 0.6919 0.2696 0.4387
Nuclear Periphery 0.0157 0.002 0.0015 0.0039 0.0046 0.0033 0.0082 0.0015 0.0019 0.0007 0.0026 0.001 0.1718 0.0022 0.0027 0.0011 0.0005 0.001
Nucleolus 0.0016 0.0006 0.0009 0.0035 0.0004 0.0006 0.001 0.0008 0.0006 0.0002 0.0001 0.0002 0.0048 0.0004 0.0013 0.0003 0.0001 0
Nucleus 0.0063 0.0019 0.0029 0.0063 0.0013 0.0022 0.0045 0.0019 0.0013 0.0006 0.0014 0.0009 0.2131 0.0015 0.0017 0.0007 0.0008 0.0004
Peroxisomes 0.0157 0.0037 0.0054 0.0166 0.0218 0.0115 0.012 0.0146 0.0019 0.0067 0.0283 0.0018 0.0003 0.0003 0.0002 0.0003 0 0
Punctate Nuclear 0.0162 0.0087 0.002 0.0117 0.0026 0.0107 0.0074 0.0015 0.0014 0.0004 0.0012 0.0016 0.0155 0.002 0.0004 0.0004 0.0004 0.0005
Vacuole 0.0075 0.0053 0.0053 0.0123 0.0034 0.0023 0.0026 0.0047 0.0042 0.0017 0.0026 0.0015 0.0099 0.0043 0.031 0.0043 0.0011 0.0015
Vacuole Periphery 0.002 0.0003 0.0007 0.0026 0.0012 0.0004 0.0008 0.0004 0.0003 0.0001 0.0004 0.0001 0.0074 0.0002 0.0027 0.0004 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.3363 10.9008 14.4288 15.7668 14.742 12.5168 15.8625 16.0843 15.6373 18.0648
Translational Efficiency 0.2692 0.2739 0.2912 0.2591 0.2091 0.3125 0.277 0.1968 0.2536 0.2112

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1920 1367 1647 1367 729 1214 1591 1757 2649 2581 3238 3124

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 768.10 756.82 866.45 865.84 613.40 789.30 825.93 821.27 725.53 772.10 846.54 840.77
Standard Deviation 89.10 115.80 114.15 104.24 76.59 97.32 112.76 102.18 110.19 108.72 115.26 105.43
Intensity Change Log 2 — -0.021344 0.173822 0.172806 — 0.363746 0.429191 0.421028 — 0.162417 0.292818 0.288318

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000449 0.001440 0.000905 0.002468 0.002013 0.000851 0.010338 0.009167 0.000880 0.001163 0.005540 0.006236
Bud Neck 0.007135 0.026951 0.004751 0.006874 0.052724 0.047875 0.016342 0.011892 0.019681 0.036793 0.010447 0.009696
Bud Site 0.019434 0.016775 0.012848 0.028135 0.015542 0.014553 0.090362 0.035960 0.018363 0.015729 0.050935 0.032536
Cell Periphery 0.000398 0.000744 0.000592 0.001024 0.004033 0.000986 0.001204 0.000977 0.001398 0.000858 0.000893 0.000998
Cytoplasm 0.501587* 0.373067* 0.528848* 0.432934* 0.420305* 0.348997* 0.473821* 0.494149* 0.479219* 0.361745* 0.501811* 0.467363*
Cytoplasmic Foci 0.079607 0.269996* 0.073462 0.082131 0.192943* 0.241217* 0.096652 0.102862 0.110797 0.256460* 0.084857 0.093791
Eisosomes 0.000180 0.001229 0.000153 0.000535 0.009364 0.001592 0.000527 0.000922 0.002708 0.001400 0.000337 0.000752
Endoplasmic Reticulum 0.002542 0.021417 0.020133 0.011528 0.026522 0.017700 0.011577 0.008783 0.009141 0.019668 0.015929 0.009985
Endosome 0.007743 0.037053 0.007035 0.007336 0.026846 0.047392 0.010598 0.006403 0.013000 0.041916 0.008786 0.006811
Golgi 0.000934 0.013829 0.000694 0.000802 0.039429 0.017573 0.002363 0.001282 0.011528 0.015590 0.001514 0.001072
Lipid Particles 0.007581 0.040766 0.009451 0.008638 0.065435 0.044316 0.015691 0.015775 0.023502 0.042436 0.012517 0.012652
Mitochondria 0.002185 0.005895 0.000280 0.001249 0.019794 0.015253 0.003729 0.000493 0.007031 0.010297 0.001974 0.000824
Mitotic Spindle 0.005409 0.002716 0.001144 0.005404 0.000068 0.002178 0.004834 0.002330 0.003939 0.002463 0.002957 0.003675
None 0.001650 0.002638 0.002700 0.001064 0.003170 0.001140 0.001642 0.001654 0.002068 0.001934 0.002180 0.001395
Nuclear Periphery 0.001559 0.000803 0.002447 0.001031 0.000609 0.000958 0.000967 0.000775 0.001297 0.000876 0.001720 0.000887
Nuclear Periphery Foci 0.001462 0.001992 0.014722 0.005787 0.000700 0.001002 0.004386 0.004356 0.001252 0.001526 0.009643 0.004982
Nucleolus 0.000537 0.001387 0.000357 0.000250 0.001549 0.000906 0.000338 0.000320 0.000816 0.001160 0.000347 0.000289
Nucleus 0.240275* 0.062478 0.225854* 0.140712* 0.047731 0.054949 0.128611 0.164234* 0.187287* 0.058936 0.178073* 0.153941*
Peroxisomes 0.002366 0.010025 0.001542 0.002073 0.016850 0.009986 0.013405 0.008423 0.006352 0.010007 0.007371 0.005644
Vacuole 0.116005 0.106179 0.091856 0.259776* 0.050466 0.126227 0.112064 0.128997 0.097969 0.115609 0.101785 0.186223
Vacuole Periphery 0.000960 0.002620 0.000225 0.000250 0.003907 0.004349 0.000549 0.000247 0.001771 0.003434 0.000384 0.000248

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.34 -2.78 -2.81 -0.93 -1.91 4.33 -5.87 -4.19 -5.01 0.85 -1.51 -6.69 -5.33 -4.97 -0.15
Bud Neck -10.68 3.35 0.61 11.16 -3.21 1.00 9.27 10.41 11.29 2.55 -8.12 7.06 7.64 14.99 0.80
Bud Site 0.66 2.20 -1.79 -2.55 -3.93 0.48 -13.69 -5.79 -6.57 9.23 1.04 -10.23 -4.97 -6.61 5.80
Cell Periphery -4.48 -4.47 -5.85 -0.79 -2.95 5.67 5.42 5.87 0.57 2.06 3.25 3.33 3.24 -0.26 -0.27
Cytoplasm 13.18 -2.91 10.10 -2.45 12.55 6.63 -3.90 -5.49 -14.34 -1.63 16.02 -2.72 4.25 -12.20 7.22
Cytoplasmic Foci -32.75* 1.68 1.82 33.74 0.18 -6.26 14.40 13.97 24.11 -1.17 -32.35 7.84 6.63 40.21 -1.45
Eisosomes -8.18 1.83 -6.82 5.95 -7.44 9.04 10.42 9.97 4.54 -3.51 4.96 9.60 8.06 6.79 -5.95
Endoplasmic Reticulum -9.02 -12.03 -7.08 5.03 5.91 2.59 4.90 5.86 4.80 2.03 -6.48 -5.29 0.06 7.02 6.10
Endosome -15.55 0.49 1.20 16.75 0.93 -8.17 9.81 13.41 20.17 5.05 -19.40 5.09 8.78 26.13 4.56
Golgi -13.84 0.81 1.09 14.22 0.25 8.57 16.81 17.35 12.43 2.98 -3.85 13.92 14.76 18.47 2.36
Lipid Particles -15.21 -2.22 0.11 15.24 2.31 4.97 12.93 12.66 11.44 0.09 -9.91 8.06 7.92 17.81 0.52
Mitochondria -4.63 3.17 1.47 6.45 -1.65 1.82 8.03 10.18 8.95 3.95 -3.09 6.63 8.85 11.02 2.70
Mitotic Spindle 1.52 3.20 -0.29 -1.77 -3.40 -2.94 -5.80 -3.25 -0.42 2.07 1.17 0.67 -0.20 -1.53 -1.01
None -1.59 -1.86 1.49 2.54 3.37 1.70 1.29 1.33 -1.21 0.11 0.05 -0.16 1.58 1.66 2.50
Nuclear Periphery 4.54 -3.80 1.89 -2.91 5.08 -1.40 -2.99 -1.94 0.46 1.45 2.99 -3.01 2.71 -1.26 5.54
Nuclear Periphery Foci -2.43 -14.82 -11.69 -10.00 9.71 -1.66 -9.94 -11.83 -10.63 0.22 -1.72 -16.84 -15.26 -14.14 8.84
Nucleolus -3.27 1.98 3.01 4.44 2.54 1.40 2.55 2.58 4.35 0.37 -1.66 3.17 3.52 5.75 1.94
Nucleus 32.09* 1.90 16.50 -15.92 14.71 -1.47 -14.09 -20.44 -25.04 -6.36 28.75 1.65 7.35 -29.08 6.58
Peroxisomes -8.11 1.30 0.97 8.87 -0.47 3.75 1.77 4.37 1.62 2.67 -4.73 -0.83 1.31 5.45 1.89
Vacuole 4.42 5.03 -22.74 -26.22 -26.49 -14.08 -15.14 -18.93 -4.29 -3.31 -2.05 -2.42 -22.36 -21.45 -20.96
Vacuole Periphery -4.48 2.21 2.23 9.80 0.17 -0.87 11.06 12.17 10.29 4.33 -5.23 5.75 6.38 14.00 3.49
Standard name
Human Ortholog
Description Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress
Localization
Cell Percentages ER (28%), vacuole (5%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ire1

Ire1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ire1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available