Standard name | |
---|---|
Human Ortholog | |
Description | SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 61 | 34 | 86 | 98 | 111 | 131 | – | 121 | 111 | 89 | 118 | 33 | 86 | 103 | 150 | 186 | 98 | 61 | 105 | 81 | 41 | 130 | 133 |
Bud | 2 | 19 | 10 | 34 | 41 | 32 | 44 | – | 50 | 36 | 29 | 44 | 6 | 26 | 26 | 32 | 32 | 19 | 3 | 5 | 8 | 10 | 23 | 25 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 7 | 7 | 8 | 22 | 23 |
Bud Site | 0 | 2 | 0 | 2 | 2 | 5 | 1 | – | 7 | 4 | 8 | 6 | 0 | 2 | 1 | 8 | 7 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 16 | 14 | 15 | 17 | 15 | 28 | – | 24 | 22 | 25 | 24 | 10 | 29 | 55 | 48 | 49 | 26 | 0 | 3 | 1 | 0 | 2 | 4 |
Cytoplasm | 1 | 44 | 25 | 43 | 64 | 52 | 81 | – | 60 | 66 | 38 | 48 | 41 | 78 | 78 | 98 | 79 | 69 | 3 | 14 | 8 | 5 | 26 | 52 |
Endoplasmic Reticulum | 1 | 22 | 7 | 27 | 18 | 41 | 57 | – | 46 | 54 | 49 | 42 | 5 | 19 | 19 | 16 | 19 | 12 | 0 | 1 | 3 | 1 | 1 | 3 |
Endosome | 0 | 0 | 0 | 0 | 12 | 2 | 15 | – | 10 | 11 | 2 | 11 | 1 | 1 | 0 | 3 | 2 | 5 | 3 | 3 | 6 | 1 | 8 | 6 |
Golgi | 0 | 3 | 2 | 2 | 7 | 1 | 2 | – | 4 | 5 | 2 | 6 | 1 | 1 | 4 | 41 | 50 | 26 | 0 | 1 | 1 | 0 | 1 | 0 |
Mitochondria | 3 | 0 | 0 | 1 | 0 | 10 | 25 | – | 56 | 48 | 98 | 76 | 0 | 0 | 1 | 16 | 8 | 2 | 2 | 1 | 4 | 1 | 9 | 5 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 1 | 0 | – | 1 | 2 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 3 | 2 | 3 | 11 | 8 | 20 | – | 14 | 10 | 4 | 18 | 3 | 9 | 8 | 7 | 7 | 7 | 0 | 1 | 2 | 1 | 0 | 0 |
SpindlePole | 0 | 2 | 1 | 3 | 12 | 1 | 18 | – | 9 | 14 | 1 | 9 | 4 | 12 | 8 | 22 | 34 | 17 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 2 | 2 | 13 | 0 | 5 | – | 6 | 9 | 1 | 6 | 1 | 1 | 0 | 12 | 15 | 12 | 0 | 0 | 0 | 0 | 2 | 1 |
Unique Cell Count | 16 | 128 | 69 | 160 | 205 | 200 | 301 | 280 | 276 | 223 | 282 | 81 | 192 | 218 | 313 | 340 | 212 | 78 | 148 | 129 | 76 | 235 | 267 | |
Labelled Cell Count | 19 | 173 | 97 | 220 | 296 | 279 | 430 | 411 | 393 | 348 | 411 | 105 | 264 | 306 | 455 | 494 | 303 | 78 | 148 | 129 | 76 | 235 | 267 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.5 | 9.9 | 9.6 | 9.2 | 9.6 | 8.2 | 8.2 | – | 7.3 | 7.5 | 7.0 | 7.4 | 9.7 | 9.7 | 9.9 | 12.9 | 13.4 | 12.8 | 8.7 | 9.0 | 9.2 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.6 | 1.5 | 1.7 | 1.5 | 1.5 | – | 1.5 | 1.6 | 1.4 | 1.7 | 1.4 | 1.4 | 1.5 | 2.5 | 2.4 | 2.4 | 1.2 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.01 | -0.24 | -0.23 | – | -0.41 | -0.35 | -0.47 | -0.39 | 0.01 | 0.01 | 0.04 | 0.42 | 0.47 | 0.41 | -0.15 | -0.1 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.6 | -0.2 | 0.9 | -0.9 | – | -0.9 | -1.4 | -1.4 | -1.1 | -1.0 | -0.6 | -0.3 | -0.2 | 0.8 | -0.4 |
Bud | 1.2 | 1.0 | 0.3 | 0 | – | 0.7 | -0.3 | -0.3 | 0.2 | -1.4 | -0.2 | -0.6 | -1.0 | -1.3 | -1.3 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.3 | -2.7 | -3.0 | -2.6 | – | -2.8 | -3.0 | -1.9 | -2.8 | -1.3 | -1.0 | 0.8 | -1.0 | -1.2 | -1.7 |
Cytoplasm | -1.4 | -0.8 | -1.6 | -1.5 | – | -2.6 | -2.1 | -3.4 | -3.5 | 1.8 | 0.6 | -0.1 | -0.8 | -2.3 | -0.6 |
Endoplasmic Reticulum | 1.3 | -0.3 | 1.9 | 1.7 | – | 1.3 | 1.8 | 2.2 | 1.0 | -0.9 | -0.1 | -0.4 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.7 | 2.3 |
Mitochondria | 0 | 0 | 0 | 0 | – | 4.1 | 3.7 | 6.8 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0953 | 3.4087 | 2.822 | 3.4015 | 1.861 | 3.2044 | 4.633 | 5.642 | 5.2213 | 4.8746 | 5.1258 | 5.4166 | 4.6398 | 5.01 | 4.4194 | 4.1453 | 4.5359 | 4.6068 |
Actin | 0.7043 | 0.5886 | 0.738 | 0.8475 | 0.8296 | 0.854 | 0.6631 | 0.4642 | 0.7192 | 0.6562 | 0.7584 | 0.7854 | 0.741 | 0.5332 | 0.7324 | 0.7239 | 0.8813 | 0.8343 |
Bud | 0.0018 | 0.0027 | 0.0298 | 0.0113 | 0.0062 | 0.0014 | 0.0032 | 0.007 | 0.0308 | 0.0333 | 0.0071 | 0.0011 | 0.0059 | 0.0076 | 0.0401 | 0.0401 | 0.0134 | 0.001 |
Bud Neck | 0.0169 | 0.0055 | 0.0127 | 0.0009 | 0.0059 | 0.1035 | 0.0234 | 0.0115 | 0.012 | 0.0021 | 0.0024 | 0.1553 | 0.044 | 0.0091 | 0.009 | 0.0011 | 0.0281 | 0.1206 |
Bud Periphery | 0.0024 | 0.002 | 0.0382 | 0.0593 | 0.0244 | 0.0023 | 0.0063 | 0.0127 | 0.064 | 0.1548 | 0.0552 | 0.0038 | 0.0077 | 0.0065 | 0.067 | 0.0962 | 0.0643 | 0.0031 |
Bud Site | 0.0607 | 0.3045 | 0.0711 | 0.0014 | 0.0023 | 0.0016 | 0.0591 | 0.341 | 0.0565 | 0.0036 | 0.0016 | 0.0032 | 0.0421 | 0.2602 | 0.0237 | 0.0069 | 0.0043 | 0.0012 |
Cell Periphery | 0.0017 | 0.0016 | 0.0007 | 0.0007 | 0.0003 | 0.0007 | 0.0044 | 0.005 | 0.0014 | 0.0011 | 0.0041 | 0.001 | 0.0061 | 0.0015 | 0.0015 | 0.0009 | 0.0028 | 0.0008 |
Cytoplasm | 0.0174 | 0.0468 | 0.0186 | 0.003 | 0.003 | 0.0171 | 0.0373 | 0.1135 | 0.0382 | 0.0436 | 0.0048 | 0.0107 | 0.0305 | 0.1223 | 0.0536 | 0.0323 | 0.002 | 0.0103 |
Cytoplasmic Foci | 0.0525 | 0.019 | 0.0127 | 0.0117 | 0.018 | 0.0097 | 0.0342 | 0.0136 | 0.0159 | 0.0159 | 0.0118 | 0.007 | 0.0305 | 0.021 | 0.0204 | 0.0205 | 0.001 | 0.0055 |
Eisosomes | 0.0017 | 0.0009 | 0.0004 | 0.0004 | 0.0008 | 0.0007 | 0.0021 | 0.0006 | 0.0013 | 0.0009 | 0.007 | 0.001 | 0.0017 | 0.0004 | 0.0009 | 0.0006 | 0.0003 | 0.0009 |
Endoplasmic Reticulum | 0.004 | 0.0009 | 0.0011 | 0.0001 | 0.0003 | 0.0005 | 0.0079 | 0.0013 | 0.0005 | 0.0005 | 0.0011 | 0.0003 | 0.0024 | 0.0014 | 0.0032 | 0.0042 | 0.0001 | 0.0017 |
Endosome | 0.0219 | 0.0061 | 0.0058 | 0.0037 | 0.0027 | 0.0004 | 0.0424 | 0.0033 | 0.0072 | 0.0076 | 0.0428 | 0.0039 | 0.0096 | 0.0048 | 0.0102 | 0.0149 | 0.0001 | 0.0041 |
Golgi | 0.0158 | 0.0066 | 0.0069 | 0.058 | 0.0109 | 0.0029 | 0.0227 | 0.0046 | 0.0138 | 0.0198 | 0.0065 | 0.0072 | 0.0114 | 0.0172 | 0.0112 | 0.0101 | 0.0011 | 0.0082 |
Lipid Particles | 0.0229 | 0.0025 | 0.0089 | 0.0001 | 0.0277 | 0.0005 | 0.0274 | 0.0005 | 0.0031 | 0.0026 | 0.0161 | 0.0063 | 0.0099 | 0.0005 | 0.0039 | 0.0076 | 0 | 0.0015 |
Mitochondria | 0.012 | 0.0043 | 0.0349 | 0.0009 | 0.0016 | 0.0003 | 0.0092 | 0.0095 | 0.0194 | 0.0476 | 0.0036 | 0.0065 | 0.0053 | 0.0005 | 0.0024 | 0.0021 | 0.0002 | 0.0008 |
None | 0.0174 | 0.0031 | 0.002 | 0.0001 | 0.0005 | 0.0008 | 0.0134 | 0.0065 | 0.0038 | 0.0021 | 0.0009 | 0.0001 | 0.0237 | 0.0007 | 0.0062 | 0.005 | 0 | 0.0002 |
Nuclear Periphery | 0.0027 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0112 | 0.0003 | 0.0002 | 0.0002 | 0.0017 | 0.0001 | 0.0056 | 0.0003 | 0.002 | 0.0036 | 0 | 0.0028 |
Nucleolus | 0.0012 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0012 | 0 | 0.0004 | 0.0001 | 0 | 0 |
Nucleus | 0.0015 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0041 | 0.0005 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0011 | 0.0003 | 0.0007 | 0.0006 | 0 | 0.0002 |
Peroxisomes | 0.0318 | 0.0038 | 0.0153 | 0.0008 | 0.064 | 0.0034 | 0.0157 | 0.003 | 0.0112 | 0.0066 | 0.0641 | 0.006 | 0.0148 | 0.012 | 0.0075 | 0.0248 | 0.0009 | 0.002 |
Punctate Nuclear | 0.0074 | 0.0002 | 0.0005 | 0 | 0.0013 | 0.0001 | 0.0036 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0037 | 0.0003 | 0.0022 | 0.0023 | 0 | 0.0004 |
Vacuole | 0.0012 | 0.0005 | 0.0007 | 0 | 0.0001 | 0 | 0.006 | 0.0008 | 0.0006 | 0.0005 | 0.0067 | 0.0006 | 0.0009 | 0.0002 | 0.001 | 0.0016 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0021 | 0.0002 | 0.0003 | 0.0005 | 0.002 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0.0006 | 0 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.6555 | 16.0766 | 18.4715 | 25.4603 | 28.9582 | 19.1336 | 25.6934 | 29.0202 | 28.5416 | 27.5424 |
Translational Efficiency | 1.2438 | 1.703 | 1.3537 | 0.9433 | 0.9443 | 1.586 | 0.9498 | 0.8001 | 0.8878 | 0.9953 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1370 | 1327 | 1070 | 866 | 2442 | 1119 | 2280 | 45 | 3812 | 2446 | 3350 | 911 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 703.27 | 965.21 | 1292.44 | 1108.26 | 927.89 | 943.26 | 1144.59 | 1078.53 | 847.16 | 955.17 | 1191.81 | 1106.79 |
Standard Deviation | 102.10 | 140.43 | 186.67 | 172.79 | 114.94 | 135.92 | 152.28 | 162.25 | 154.36 | 138.82 | 177.95 | 172.41 |
Intensity Change Log 2 | — | 0.456764 | 0.877947 | 0.656146 | — | 0.023702 | 0.302805 | 0.217041 | — | 0.226518 | 0.579226 | 0.422916 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.561366* | 0.726622* | 0.538374* | 0.747793* | 0.687006* | 0.631949* | 0.594860* | 0.475583* | 0.641852* | 0.683310* | 0.576818* | 0.734346* |
Bud Neck | 0.017456 | 0.013543 | 0.019615 | 0.010901 | 0.029910 | 0.022221 | 0.016535 | 0.016187 | 0.025434 | 0.017513 | 0.017519 | 0.011162 |
Bud Site | 0.077382 | 0.118576* | 0.282982* | 0.110647* | 0.151820* | 0.188148* | 0.313458* | 0.072674 | 0.125067* | 0.150404* | 0.303724* | 0.108771* |
Cell Periphery | 0.012942 | 0.007460 | 0.004873 | 0.043384 | 0.018090 | 0.018981 | 0.007468 | 0.009064 | 0.016240 | 0.012731 | 0.006639 | 0.041689 |
Cytoplasm | 0.040881 | 0.040650 | 0.030595 | 0.011157 | 0.035537 | 0.038681 | 0.043183 | 0.012733 | 0.037458 | 0.039749 | 0.039162 | 0.011235 |
Cytoplasmic Foci | 0.060059 | 0.018623 | 0.021771 | 0.002403 | 0.016891 | 0.020032 | 0.002868 | 0.070401 | 0.032405 | 0.019268 | 0.008906 | 0.005762 |
Eisosomes | 0.019448 | 0.006876 | 0.003834 | 0.042846 | 0.011742 | 0.007626 | 0.002656 | 0.030598 | 0.014511 | 0.007219 | 0.003032 | 0.042241 |
Endoplasmic Reticulum | 0.000832 | 0.000400 | 0.004346 | 0.003189 | 0.000218 | 0.001259 | 0.000547 | 0.020517 | 0.000439 | 0.000793 | 0.001760 | 0.004045 |
Endosome | 0.007320 | 0.007440 | 0.022511 | 0.004494 | 0.001773 | 0.006087 | 0.001576 | 0.049417 | 0.003767 | 0.006821 | 0.008263 | 0.006714 |
Golgi | 0.019136 | 0.010857 | 0.011454 | 0.004722 | 0.004487 | 0.011622 | 0.001251 | 0.039347 | 0.009751 | 0.011207 | 0.004510 | 0.006432 |
Lipid Particles | 0.007747 | 0.001760 | 0.015526 | 0.001751 | 0.001189 | 0.001952 | 0.000525 | 0.101815* | 0.003546 | 0.001848 | 0.005316 | 0.006694 |
Mitochondria | 0.011645 | 0.002547 | 0.000883 | 0.003645 | 0.002454 | 0.006643 | 0.000876 | 0.000398 | 0.005757 | 0.004421 | 0.000879 | 0.003484 |
Mitotic Spindle | 0.000200 | 0.000663 | 0.005224 | 0.000823 | 0.000302 | 0.001762 | 0.000930 | 0.002200 | 0.000265 | 0.001166 | 0.002302 | 0.000891 |
None | 0.000365 | 0.000028 | 0.000017 | 0.000043 | 0.000090 | 0.000041 | 0.000030 | 0.000012 | 0.000189 | 0.000034 | 0.000026 | 0.000042 |
Nuclear Periphery | 0.000039 | 0.000002 | 0.000048 | 0.000033 | 0.000002 | 0.000005 | 0.000005 | 0.000042 | 0.000016 | 0.000003 | 0.000019 | 0.000034 |
Nuclear Periphery Foci | 0.000106 | 0.000037 | 0.000447 | 0.000146 | 0.000028 | 0.000046 | 0.000026 | 0.001220 | 0.000056 | 0.000041 | 0.000160 | 0.000199 |
Nucleolus | 0.000252 | 0.000004 | 0.000011 | 0.000005 | 0.000005 | 0.000007 | 0.000002 | 0.000019 | 0.000094 | 0.000005 | 0.000005 | 0.000006 |
Nucleus | 0.002649 | 0.000189 | 0.000627 | 0.000159 | 0.000407 | 0.000196 | 0.000195 | 0.000525 | 0.001213 | 0.000192 | 0.000333 | 0.000177 |
Peroxisomes | 0.159142* | 0.041615 | 0.021502 | 0.004143 | 0.036824 | 0.039949 | 0.010737 | 0.069757 | 0.080784 | 0.040853 | 0.014175 | 0.007384 |
Vacuole | 0.000816 | 0.001707 | 0.015081 | 0.005246 | 0.001163 | 0.002397 | 0.002171 | 0.027225 | 0.001038 | 0.002023 | 0.006295 | 0.006331 |
Vacuole Periphery | 0.000218 | 0.000402 | 0.000280 | 0.002470 | 0.000061 | 0.000397 | 0.000101 | 0.000266 | 0.000117 | 0.000400 | 0.000158 | 0.002361 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -12.22 | 2.80 | -12.45 | -1.25 | -14.15 | 4.30 | 9.22 | 3.67 | 2.71 | 2.04 | -4.51 | 8.52 | -7.23 | -3.67 | -12.88 |
Bud Neck | 1.74 | -0.67 | 3.10 | 1.35 | 3.82 | 2.70 | 6.09 | 1.65 | 0.71 | 0.01 | 4.25 | 4.93 | 7.62 | 3.51 | 3.82 |
Bud Site | -5.34 | -18.27 | -3.94 | 0.80 | 13.89 | -3.74 | -18.73 | 3.58 | 5.02 | 10.69 | -4.05 | -25.99 | 2.03 | 4.87 | 21.78* |
Cell Periphery | 3.76 | 5.90 | -7.57 | -9.24 | -10.01 | -0.37 | 7.92 | 2.51 | 2.50 | -0.44 | 2.59 | 9.65 | -6.78 | -7.64 | -9.58 |
Cytoplasm | 0.04 | 0.22 | 7.83 | 7.40 | 6.83 | -0.92 | -3.42 | 4.71 | 4.86 | 6.63 | -0.87 | -2.66 | 9.32 | 8.97 | 10.81 |
Cytoplasmic Foci | 10.61 | 8.94 | 16.31 | 8.67 | 10.30 | -1.28 | 9.66 | -2.29 | -2.14 | -2.87 | 6.24 | 13.11 | 12.76 | 6.83 | 2.51 |
Eisosomes | 6.71 | 8.48 | -5.26 | -8.65 | -9.44 | 3.47 | 10.95 | -1.10 | -1.35 | -1.65 | 7.40 | 13.57 | -6.83 | -8.72 | -9.86 |
Endoplasmic Reticulum | 1.24 | -3.87 | -2.63 | -3.38 | 0.98 | -1.43 | -1.88 | -1.85 | -1.75 | -1.82 | -0.96 | -4.13 | -3.74 | -3.22 | -2.28 |
Endosome | -0.10 | -6.03 | 1.93 | 1.96 | 6.88 | -4.74 | 0.34 | -3.39 | -3.08 | -3.40 | -4.10 | -5.11 | -2.21 | 0.02 | 0.92 |
Golgi | 4.12 | 3.91 | 6.85 | 3.24 | 3.17 | -4.77 | 5.36 | -2.44 | -1.92 | -2.68 | -1.28 | 6.52 | 2.03 | 2.68 | -1.27 |
Lipid Particles | 4.63 | -3.07 | 4.46 | -0.07 | 5.88 | -1.06 | 1.97 | -3.02 | -3.00 | -3.04 | 2.73 | -2.10 | -1.66 | -2.56 | -0.66 |
Mitochondria | 7.00 | 8.99 | 4.28 | -0.69 | -1.87 | -2.81 | 3.28 | 4.06 | 4.36 | 1.92 | 1.51 | 9.14 | 1.55 | 0.61 | -1.87 |
Mitotic Spindle | -1.10 | -3.68 | -1.98 | -0.41 | 3.18 | -1.55 | -1.96 | -1.13 | -0.36 | -0.79 | -1.83 | -4.16 | -1.98 | 0.33 | 2.50 |
None | 5.36 | 5.37 | 5.05 | -1.38 | -1.40 | 4.00 | 4.32 | 6.81 | 4.49 | 2.19 | 6.48 | 6.51 | 5.70 | -0.72 | -0.99 |
Nuclear Periphery | 1.58 | -0.62 | 0.19 | -2.91 | 0.96 | -1.50 | -2.76 | -1.86 | -1.76 | -1.69 | 1.46 | -0.70 | -1.41 | -2.98 | -0.81 |
Nuclear Periphery Foci | 1.37 | -4.37 | -0.35 | -2.50 | 4.47 | -1.16 | -0.81 | -2.28 | -2.24 | -2.26 | 0.94 | -4.17 | -2.56 | -3.23 | 0.45 |
Nucleolus | 1.89 | 1.80 | 1.87 | -1.06 | 2.89 | -0.79 | 3.82 | -1.85 | -1.48 | -2.26 | 1.87 | 1.85 | 1.85 | -0.38 | -0.03 |
Nucleus | 2.85 | 1.29 | 2.84 | -0.09 | 3.15 | 3.06 | 2.18 | -0.58 | -1.46 | -1.20 | 3.24 | 1.71 | 3.17 | -0.48 | 3.21 |
Peroxisomes | 18.13* | 21.98 | 26.31* | 12.59 | 7.05 | -0.85 | 11.17 | -1.39 | -1.25 | -2.52 | 11.77 | 23.36 | 24.19 | 13.00 | 3.78 |
Vacuole | -1.74 | -8.08 | -3.46 | -3.00 | 5.01 | -1.96 | -3.41 | -2.08 | -2.00 | -1.89 | -2.43 | -8.62 | -3.90 | -3.38 | 0.94 |
Vacuole Periphery | -0.79 | -0.64 | -2.14 | -1.93 | -2.08 | -2.23 | -0.72 | -1.36 | 0.61 | -1.03 | -1.99 | -0.83 | -2.25 | -1.95 | -2.21 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|