Standard name
Human Ortholog
Description SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.75 0.48 0.49 0.54 0.48 0.56 0.44 – 0.43 0.4 0.4 0.42 0.41 0.45 0.47 0.48 0.55 0.46 0.79 0.71 0.63 0.55 0.56 0.5
Bud 0.12 0.15 0.14 0.21 0.2 0.16 0.15 – 0.18 0.13 0.13 0.16 0.07 0.14 0.12 0.1 0.09 0.09 0.05 0 0.07 0.13 0.1 0.1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.11 0.09 0.09
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.12 0.2 0.09 0.08 0.07 0.09 – 0.09 0.08 0.11 0.09 0.12 0.15 0.25 0.15 0.14 0.12 0 0 0 0 0 0
Cytoplasm 0.06 0.34 0.36 0.27 0.31 0.26 0.27 – 0.21 0.24 0.17 0.17 0.51 0.41 0.36 0.31 0.23 0.33 0 0.1 0.07 0.08 0.11 0.2
Endoplasmic Reticulum 0.06 0.17 0.1 0.17 0.09 0.2 0.19 – 0.16 0.2 0.22 0.15 0.06 0.1 0.09 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.13 0.15 0.12 0 0 0 0 0 0
Mitochondria 0.19 0 0 0 0 0.05 0.08 – 0.2 0.17 0.44 0.27 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0.07 – 0.05 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0.06 – 0 0.05 0 0 0.05 0.06 0 0.07 0.1 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 61 34 86 98 111 131 – 121 111 89 118 33 86 103 150 186 98 61 105 81 41 130 133
Bud 2 19 10 34 41 32 44 – 50 36 29 44 6 26 26 32 32 19 3 5 8 10 23 25
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 3 0 2 7 7 8 22 23
Bud Site 0 2 0 2 2 5 1 – 7 4 8 6 0 2 1 8 7 3 – – – – – –
Cell Periphery 0 16 14 15 17 15 28 – 24 22 25 24 10 29 55 48 49 26 0 3 1 0 2 4
Cytoplasm 1 44 25 43 64 52 81 – 60 66 38 48 41 78 78 98 79 69 3 14 8 5 26 52
Endoplasmic Reticulum 1 22 7 27 18 41 57 – 46 54 49 42 5 19 19 16 19 12 0 1 3 1 1 3
Endosome 0 0 0 0 12 2 15 – 10 11 2 11 1 1 0 3 2 5 3 3 6 1 8 6
Golgi 0 3 2 2 7 1 2 – 4 5 2 6 1 1 4 41 50 26 0 1 1 0 1 0
Mitochondria 3 0 0 1 0 10 25 – 56 48 98 76 0 0 1 16 8 2 2 1 4 1 9 5
Nucleus 0 1 0 1 0 1 0 – 1 2 1 1 0 0 1 2 0 6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 – 2 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 2 – 1 0 1 2 0 0 0 0 3 1 0 0 0 0 0 0
Peroxisomes 0 3 2 3 11 8 20 – 14 10 4 18 3 9 8 7 7 7 0 1 2 1 0 0
SpindlePole 0 2 1 3 12 1 18 – 9 14 1 9 4 12 8 22 34 17 0 0 0 0 1 1
Vac/Vac Membrane 0 0 2 2 13 0 5 – 6 9 1 6 1 1 0 12 15 12 0 0 0 0 2 1
Unique Cell Count 16 128 69 160 205 200 301 280 276 223 282 81 192 218 313 340 212 78 148 129 76 235 267
Labelled Cell Count 19 173 97 220 296 279 430 411 393 348 411 105 264 306 455 494 303 78 148 129 76 235 267


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 9.9 9.6 9.2 9.6 8.2 8.2 – 7.3 7.5 7.0 7.4 9.7 9.7 9.9 12.9 13.4 12.8 8.7 9.0 9.2
Std Deviation (1e-4) 1.1 1.5 1.6 1.5 1.7 1.5 1.5 – 1.5 1.6 1.4 1.7 1.4 1.4 1.5 2.5 2.4 2.4 1.2 1.4 1.4
Intensity Change (Log2) – – – -0.08 -0.01 -0.24 -0.23 – -0.41 -0.35 -0.47 -0.39 0.01 0.01 0.04 0.42 0.47 0.41 -0.15 -0.1 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -0.2 0.9 -0.9 – -0.9 -1.4 -1.4 -1.1 -1.0 -0.6 -0.3 -0.2 0.8 -0.4
Bud 1.2 1.0 0.3 0 – 0.7 -0.3 -0.3 0.2 -1.4 -0.2 -0.6 -1.0 -1.3 -1.3
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.3 -2.7 -3.0 -2.6 – -2.8 -3.0 -1.9 -2.8 -1.3 -1.0 0.8 -1.0 -1.2 -1.7
Cytoplasm -1.4 -0.8 -1.6 -1.5 – -2.6 -2.1 -3.4 -3.5 1.8 0.6 -0.1 -0.8 -2.3 -0.6
Endoplasmic Reticulum 1.3 -0.3 1.9 1.7 – 1.3 1.8 2.2 1.0 -0.9 -0.1 -0.4 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 2.4 2.7 2.3
Mitochondria 0 0 0 0 – 4.1 3.7 6.8 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 2.3 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0953 3.4087 2.822 3.4015 1.861 3.2044 4.633 5.642 5.2213 4.8746 5.1258 5.4166 4.6398 5.01 4.4194 4.1453 4.5359 4.6068
Actin 0.7043 0.5886 0.738 0.8475 0.8296 0.854 0.6631 0.4642 0.7192 0.6562 0.7584 0.7854 0.741 0.5332 0.7324 0.7239 0.8813 0.8343
Bud 0.0018 0.0027 0.0298 0.0113 0.0062 0.0014 0.0032 0.007 0.0308 0.0333 0.0071 0.0011 0.0059 0.0076 0.0401 0.0401 0.0134 0.001
Bud Neck 0.0169 0.0055 0.0127 0.0009 0.0059 0.1035 0.0234 0.0115 0.012 0.0021 0.0024 0.1553 0.044 0.0091 0.009 0.0011 0.0281 0.1206
Bud Periphery 0.0024 0.002 0.0382 0.0593 0.0244 0.0023 0.0063 0.0127 0.064 0.1548 0.0552 0.0038 0.0077 0.0065 0.067 0.0962 0.0643 0.0031
Bud Site 0.0607 0.3045 0.0711 0.0014 0.0023 0.0016 0.0591 0.341 0.0565 0.0036 0.0016 0.0032 0.0421 0.2602 0.0237 0.0069 0.0043 0.0012
Cell Periphery 0.0017 0.0016 0.0007 0.0007 0.0003 0.0007 0.0044 0.005 0.0014 0.0011 0.0041 0.001 0.0061 0.0015 0.0015 0.0009 0.0028 0.0008
Cytoplasm 0.0174 0.0468 0.0186 0.003 0.003 0.0171 0.0373 0.1135 0.0382 0.0436 0.0048 0.0107 0.0305 0.1223 0.0536 0.0323 0.002 0.0103
Cytoplasmic Foci 0.0525 0.019 0.0127 0.0117 0.018 0.0097 0.0342 0.0136 0.0159 0.0159 0.0118 0.007 0.0305 0.021 0.0204 0.0205 0.001 0.0055
Eisosomes 0.0017 0.0009 0.0004 0.0004 0.0008 0.0007 0.0021 0.0006 0.0013 0.0009 0.007 0.001 0.0017 0.0004 0.0009 0.0006 0.0003 0.0009
Endoplasmic Reticulum 0.004 0.0009 0.0011 0.0001 0.0003 0.0005 0.0079 0.0013 0.0005 0.0005 0.0011 0.0003 0.0024 0.0014 0.0032 0.0042 0.0001 0.0017
Endosome 0.0219 0.0061 0.0058 0.0037 0.0027 0.0004 0.0424 0.0033 0.0072 0.0076 0.0428 0.0039 0.0096 0.0048 0.0102 0.0149 0.0001 0.0041
Golgi 0.0158 0.0066 0.0069 0.058 0.0109 0.0029 0.0227 0.0046 0.0138 0.0198 0.0065 0.0072 0.0114 0.0172 0.0112 0.0101 0.0011 0.0082
Lipid Particles 0.0229 0.0025 0.0089 0.0001 0.0277 0.0005 0.0274 0.0005 0.0031 0.0026 0.0161 0.0063 0.0099 0.0005 0.0039 0.0076 0 0.0015
Mitochondria 0.012 0.0043 0.0349 0.0009 0.0016 0.0003 0.0092 0.0095 0.0194 0.0476 0.0036 0.0065 0.0053 0.0005 0.0024 0.0021 0.0002 0.0008
None 0.0174 0.0031 0.002 0.0001 0.0005 0.0008 0.0134 0.0065 0.0038 0.0021 0.0009 0.0001 0.0237 0.0007 0.0062 0.005 0 0.0002
Nuclear Periphery 0.0027 0.0001 0.0003 0 0.0001 0 0.0112 0.0003 0.0002 0.0002 0.0017 0.0001 0.0056 0.0003 0.002 0.0036 0 0.0028
Nucleolus 0.0012 0.0001 0.0006 0 0 0 0.0011 0.0001 0.0001 0 0.0007 0.0001 0.0012 0 0.0004 0.0001 0 0
Nucleus 0.0015 0.0001 0.0004 0 0 0 0.0041 0.0005 0.0002 0.0002 0.0008 0.0001 0.0011 0.0003 0.0007 0.0006 0 0.0002
Peroxisomes 0.0318 0.0038 0.0153 0.0008 0.064 0.0034 0.0157 0.003 0.0112 0.0066 0.0641 0.006 0.0148 0.012 0.0075 0.0248 0.0009 0.002
Punctate Nuclear 0.0074 0.0002 0.0005 0 0.0013 0.0001 0.0036 0.0003 0.0004 0.0001 0.0005 0.0001 0.0037 0.0003 0.0022 0.0023 0 0.0004
Vacuole 0.0012 0.0005 0.0007 0 0.0001 0 0.006 0.0008 0.0006 0.0005 0.0067 0.0006 0.0009 0.0002 0.001 0.0016 0.0001 0.0001
Vacuole Periphery 0.0009 0.0001 0.0004 0 0.0001 0 0.0021 0.0002 0.0003 0.0005 0.002 0.0003 0.0009 0.0001 0.0003 0.0006 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.6555 16.0766 18.4715 25.4603 28.9582 19.1336 25.6934 29.0202 28.5416 27.5424
Translational Efficiency 1.2438 1.703 1.3537 0.9433 0.9443 1.586 0.9498 0.8001 0.8878 0.9953

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins
Localization
Cell Percentages actin (86%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bzz1

Bzz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bzz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available