Standard name | |
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Human Ortholog | |
Description | Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 5 | 0 | 1 | 2 |
Bud | 1 | 0 | 1 | 1 | 0 | 12 | 14 | 9 | 15 | 21 | 25 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 0 | 6 | 10 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 3 | 3 | 1 | 1 | 3 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 4 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 7 | 7 | 0 | 4 | 3 | 4 | 2 | 1 | 3 | 1 | 3 | 3 | 2 | 10 | 4 | 4 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 196 | 263 | 314 | 260 | 302 | 393 | 487 | 449 | 307 | 371 | 274 | 337 | 432 | 428 | 461 | 215 | 203 | 154 | 237 | 241 | 250 | 179 | 371 | 312 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 13 | 17 | 11 | 3 | 0 | 4 | 1 | 2 | 0 |
Endosome | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 13 | 5 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 2 | 4 | 0 | 1 | 4 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 16 | 7 | 5 | 13 | 25 | 122 | 215 | 160 | 175 | 234 | 215 | 245 | 0 | 0 | 0 | 0 | 8 | 3 | 13 | 5 | 27 | 3 | 11 | 5 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 2 | 4 | 2 | 3 | 2 | 1 | 0 | 1 | 1 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 13 | 10 | 6 | 10 | 11 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 6 | 5 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 1 | 3 | 5 | 2 | 3 | 4 | 9 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 3 | 1 | 6 | 4 |
Unique Cell Count | 207 | 268 | 323 | 265 | 309 | 441 | 578 | 552 | 414 | 494 | 409 | 491 | 433 | 429 | 462 | 221 | 210 | 159 | 278 | 263 | 322 | 194 | 417 | 354 |
Labelled Cell Count | 217 | 271 | 335 | 285 | 328 | 546 | 738 | 660 | 525 | 648 | 545 | 634 | 440 | 438 | 466 | 242 | 238 | 178 | 278 | 263 | 322 | 194 | 417 | 354 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 4.3 | 4.3 | 4.3 | 4.2 | 3.6 | 3.5 | 3.5 | 3.4 | 3.1 | 2.9 | 3.1 | 5.8 | 5.6 | 5.3 | 6.1 | 5.5 | 5.7 | 5.7 | 5.8 | 6.1 |
Std Deviation (1e-4) | 0.3 | 0.6 | 1.4 | 1.9 | 1.9 | 1.9 | 2.0 | 3.7 | 1.7 | 1.0 | 1.1 | 2.6 | 0.8 | 0.8 | 1.0 | 2.2 | 1.5 | 1.9 | 1.0 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.0 | -0.03 | -0.25 | -0.29 | -0.29 | -0.34 | -0.48 | -0.58 | -0.46 | 0.42 | 0.38 | 0.31 | 0.5 | 0.35 | 0.4 | 0.41 | 0.44 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5557 | -0.2265 | -0.2723 | -0.3237 | -0.5608 | -0.2656 | 0.8017 | 0.6155 | 0.6304 | 0.6267 | 1.2483 | 0.745 | -0.2744 | -0.1529 | -0.4253 | 0.0348 | 0.0279 | 0.0227 |
Actin | 0.0017 | 0.0006 | 0.0057 | 0.0002 | 0.0003 | 0.0003 | 0.0313 | 0.0006 | 0.0123 | 0.0031 | 0.0066 | 0.0046 | 0.0192 | 0.0007 | 0.0024 | 0.029 | 0.0297 | 0.0091 |
Bud | 0.0001 | 0.0025 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0088 | 0.0053 | 0.002 | 0.001 | 0.0481 | 0.0036 | 0.0072 | 0.0155 | 0.0154 | 0.0018 | 0.0128 | 0.0021 |
Bud Neck | 0.0079 | 0.0002 | 0.0009 | 0 | 0.0001 | 0.0003 | 0.0055 | 0.0003 | 0.0048 | 0.0011 | 0.0234 | 0.0067 | 0.0009 | 0.0003 | 0.0009 | 0.001 | 0.0008 | 0.0022 |
Bud Periphery | 0.0001 | 0.0005 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0094 | 0.004 | 0.0021 | 0.0018 | 0.0138 | 0.0049 | 0.0025 | 0.0032 | 0.0071 | 0.0013 | 0.01 | 0.0015 |
Bud Site | 0.0014 | 0.0045 | 0.0041 | 0 | 0 | 0 | 0.0082 | 0.0139 | 0.0142 | 0.0036 | 0.0288 | 0.0013 | 0.003 | 0.0278 | 0.0072 | 0.0014 | 0.0009 | 0.0013 |
Cell Periphery | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0021 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.1124 | 0.127 | 0.1338 | 0.0858 | 0.1858 | 0.1673 | 0.1152 | 0.1969 | 0.1824 | 0.2667 | 0.1589 | 0.2612 | 0.1823 | 0.2867 | 0.28 | 0.2282 | 0.2484 | 0.3298 |
Cytoplasmic Foci | 0.0162 | 0.0071 | 0.0071 | 0.0044 | 0.0073 | 0.0091 | 0.0094 | 0.0064 | 0.0103 | 0.0119 | 0.048 | 0.0124 | 0.0209 | 0.0065 | 0.0081 | 0.0159 | 0.0184 | 0.0035 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.001 | 0.0009 | 0.0007 | 0.0005 | 0.0007 | 0.0007 | 0.0035 | 0.0008 | 0.0012 | 0.0014 | 0.001 | 0.001 | 0.003 | 0.0017 | 0.001 | 0.0042 | 0.0026 | 0.0013 |
Endosome | 0.0031 | 0.0014 | 0.0013 | 0.0015 | 0.0037 | 0.0019 | 0.009 | 0.0017 | 0.0029 | 0.0124 | 0.0054 | 0.0032 | 0.0061 | 0.0025 | 0.0011 | 0.0208 | 0.0177 | 0.0019 |
Golgi | 0.0006 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0004 | 0.0029 | 0.0002 | 0.0012 | 0.0085 | 0.0045 | 0.0007 | 0.0024 | 0.0005 | 0.0002 | 0.003 | 0.0091 | 0.0014 |
Lipid Particles | 0.0022 | 0.0009 | 0.0014 | 0.0001 | 0.0001 | 0.0023 | 0.0045 | 0.0002 | 0.005 | 0.0107 | 0.0079 | 0.0007 | 0.0062 | 0.0015 | 0.0002 | 0.0022 | 0.0231 | 0.0009 |
Mitochondria | 0.0004 | 0.0005 | 0.004 | 0.0002 | 0.0003 | 0.0028 | 0.0021 | 0.0007 | 0.0009 | 0.0045 | 0.0041 | 0.0031 | 0.0013 | 0.0011 | 0.0015 | 0.0037 | 0.0029 | 0.0018 |
None | 0.8421 | 0.8303 | 0.8267 | 0.9046 | 0.7989 | 0.8106 | 0.7637 | 0.7596 | 0.743 | 0.6626 | 0.6041 | 0.6853 | 0.7246 | 0.6444 | 0.6615 | 0.6593 | 0.5704 | 0.6376 |
Nuclear Periphery | 0.0008 | 0.0004 | 0.0007 | 0.0003 | 0.0003 | 0.0003 | 0.0058 | 0.0004 | 0.0013 | 0.0015 | 0.0016 | 0.0015 | 0.0036 | 0.0008 | 0.001 | 0.0048 | 0.0084 | 0.0015 |
Nucleolus | 0.0009 | 0.001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0021 | 0.0008 | 0.0007 | 0.0006 | 0.0037 | 0.0003 | 0.0008 | 0.0005 | 0.0008 | 0.0016 | 0.0014 | 0.0002 |
Nucleus | 0.0016 | 0.0062 | 0.0017 | 0.0005 | 0.0007 | 0.0006 | 0.0039 | 0.0031 | 0.0049 | 0.0021 | 0.0177 | 0.0023 | 0.0038 | 0.003 | 0.0035 | 0.0092 | 0.0052 | 0.0016 |
Peroxisomes | 0.004 | 0.0003 | 0.0067 | 0.0001 | 0.0001 | 0.0007 | 0.0051 | 0.0002 | 0.0045 | 0.0012 | 0.0095 | 0.0013 | 0.0031 | 0.0003 | 0.0002 | 0.0014 | 0.0136 | 0.0005 |
Punctate Nuclear | 0.0024 | 0.0144 | 0.0014 | 0.0003 | 0.0005 | 0.0019 | 0.0034 | 0.0032 | 0.0044 | 0.0018 | 0.0107 | 0.0048 | 0.0068 | 0.0012 | 0.006 | 0.0079 | 0.0213 | 0.0011 |
Vacuole | 0.0006 | 0.0007 | 0.0004 | 0.0009 | 0.0004 | 0.0004 | 0.0029 | 0.0013 | 0.001 | 0.0025 | 0.0013 | 0.0007 | 0.0012 | 0.001 | 0.0011 | 0.002 | 0.0018 | 0.0004 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0009 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0008 | 0.001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.5476 | 24.4426 | 25.1584 | 37.0454 | 29.1612 | 17.6306 | 32.0882 | 38.7411 | 33.9851 | 35.6383 |
Translational Efficiency | 0.9684 | 1.2584 | 1.2134 | 0.7187 | 0.9115 | 1.8656 | 0.9551 | 0.9273 | 1.0325 | 1.0048 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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