Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.98 0.98 0.89 0.84 0.81 0.74 0.75 0.67 0.69 1.0 1.0 1.0 0.97 0.97 0.97 0.85 0.92 0.78 0.92 0.89 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.05 0.08 0.28 0.37 0.29 0.42 0.47 0.53 0.5 0 0 0 0 0 0 0.05 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 5 0 1 2
Bud 1 0 1 1 0 12 14 9 15 21 25 25 0 0 0 0 0 0 1 1 6 0 6 10
Bud Neck 0 0 1 0 0 1 1 3 3 3 1 1 3 4 0 0 0 1 1 0 2 0 1 1
Bud Site 0 0 0 0 0 0 1 3 1 4 4 5 0 0 0 0 0 0
Cell Periphery 2 1 7 7 0 4 3 4 2 1 3 1 3 3 2 10 4 4 0 0 1 0 1 0
Cytoplasm 196 263 314 260 302 393 487 449 307 371 274 337 432 428 461 215 203 154 237 241 250 179 371 312
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 1 2 0 1 1 13 17 11 3 0 4 1 2 0
Endosome 1 0 1 0 0 1 1 13 5 1 0 0 1 0 0 2 1 1 2 2 4 0 1 4
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 0 0 1 0 0 0
Mitochondria 16 7 5 13 25 122 215 160 175 234 215 245 0 0 0 0 8 3 13 5 27 3 11 5
Nucleus 0 0 1 1 0 2 4 2 3 2 1 0 1 1 2 0 3 0 0 0 0 1 1 0
Nuclear Periphery 1 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 0 2 2 1 0 0 1 2 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 5 5 13 10 6 10 11 0 1 0 0 0 0 1 1 3 1 6 5
Vac/Vac Membrane 0 0 1 1 1 3 5 2 3 4 9 3 0 0 0 1 0 1 2 2 3 1 6 4
Unique Cell Count 207 268 323 265 309 441 578 552 414 494 409 491 433 429 462 221 210 159 278 263 322 194 417 354
Labelled Cell Count 217 271 335 285 328 546 738 660 525 648 545 634 440 438 466 242 238 178 278 263 322 194 417 354


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.3 4.3 4.3 4.2 3.6 3.5 3.5 3.4 3.1 2.9 3.1 5.8 5.6 5.3 6.1 5.5 5.7 5.7 5.8 6.1
Std Deviation (1e-4) 0.3 0.6 1.4 1.9 1.9 1.9 2.0 3.7 1.7 1.0 1.1 2.6 0.8 0.8 1.0 2.2 1.5 1.9 1.0 1.3 1.6
Intensity Change (Log2) -0.0 -0.03 -0.25 -0.29 -0.29 -0.34 -0.48 -0.58 -0.46 0.42 0.38 0.31 0.5 0.35 0.4 0.41 0.44 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5557 -0.2265 -0.2723 -0.3237 -0.5608 -0.2656 0.8017 0.6155 0.6304 0.6267 1.2483 0.745 -0.2744 -0.1529 -0.4253 0.0348 0.0279 0.0227
Actin 0.0017 0.0006 0.0057 0.0002 0.0003 0.0003 0.0313 0.0006 0.0123 0.0031 0.0066 0.0046 0.0192 0.0007 0.0024 0.029 0.0297 0.0091
Bud 0.0001 0.0025 0.0011 0.0001 0.0001 0 0.0088 0.0053 0.002 0.001 0.0481 0.0036 0.0072 0.0155 0.0154 0.0018 0.0128 0.0021
Bud Neck 0.0079 0.0002 0.0009 0 0.0001 0.0003 0.0055 0.0003 0.0048 0.0011 0.0234 0.0067 0.0009 0.0003 0.0009 0.001 0.0008 0.0022
Bud Periphery 0.0001 0.0005 0.0008 0.0001 0.0002 0 0.0094 0.004 0.0021 0.0018 0.0138 0.0049 0.0025 0.0032 0.0071 0.0013 0.01 0.0015
Bud Site 0.0014 0.0045 0.0041 0 0 0 0.0082 0.0139 0.0142 0.0036 0.0288 0.0013 0.003 0.0278 0.0072 0.0014 0.0009 0.0013
Cell Periphery 0.0001 0.0002 0.0002 0 0 0 0.0021 0.0004 0.0003 0.0004 0.0005 0.0001 0.0004 0.0005 0.0005 0.0002 0.0001 0.0001
Cytoplasm 0.1124 0.127 0.1338 0.0858 0.1858 0.1673 0.1152 0.1969 0.1824 0.2667 0.1589 0.2612 0.1823 0.2867 0.28 0.2282 0.2484 0.3298
Cytoplasmic Foci 0.0162 0.0071 0.0071 0.0044 0.0073 0.0091 0.0094 0.0064 0.0103 0.0119 0.048 0.0124 0.0209 0.0065 0.0081 0.0159 0.0184 0.0035
Eisosomes 0.0002 0.0001 0.0002 0 0 0 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.001 0.0009 0.0007 0.0005 0.0007 0.0007 0.0035 0.0008 0.0012 0.0014 0.001 0.001 0.003 0.0017 0.001 0.0042 0.0026 0.0013
Endosome 0.0031 0.0014 0.0013 0.0015 0.0037 0.0019 0.009 0.0017 0.0029 0.0124 0.0054 0.0032 0.0061 0.0025 0.0011 0.0208 0.0177 0.0019
Golgi 0.0006 0.0002 0.0008 0.0001 0.0001 0.0004 0.0029 0.0002 0.0012 0.0085 0.0045 0.0007 0.0024 0.0005 0.0002 0.003 0.0091 0.0014
Lipid Particles 0.0022 0.0009 0.0014 0.0001 0.0001 0.0023 0.0045 0.0002 0.005 0.0107 0.0079 0.0007 0.0062 0.0015 0.0002 0.0022 0.0231 0.0009
Mitochondria 0.0004 0.0005 0.004 0.0002 0.0003 0.0028 0.0021 0.0007 0.0009 0.0045 0.0041 0.0031 0.0013 0.0011 0.0015 0.0037 0.0029 0.0018
None 0.8421 0.8303 0.8267 0.9046 0.7989 0.8106 0.7637 0.7596 0.743 0.6626 0.6041 0.6853 0.7246 0.6444 0.6615 0.6593 0.5704 0.6376
Nuclear Periphery 0.0008 0.0004 0.0007 0.0003 0.0003 0.0003 0.0058 0.0004 0.0013 0.0015 0.0016 0.0015 0.0036 0.0008 0.001 0.0048 0.0084 0.0015
Nucleolus 0.0009 0.001 0.0003 0.0001 0.0001 0.0001 0.0021 0.0008 0.0007 0.0006 0.0037 0.0003 0.0008 0.0005 0.0008 0.0016 0.0014 0.0002
Nucleus 0.0016 0.0062 0.0017 0.0005 0.0007 0.0006 0.0039 0.0031 0.0049 0.0021 0.0177 0.0023 0.0038 0.003 0.0035 0.0092 0.0052 0.0016
Peroxisomes 0.004 0.0003 0.0067 0.0001 0.0001 0.0007 0.0051 0.0002 0.0045 0.0012 0.0095 0.0013 0.0031 0.0003 0.0002 0.0014 0.0136 0.0005
Punctate Nuclear 0.0024 0.0144 0.0014 0.0003 0.0005 0.0019 0.0034 0.0032 0.0044 0.0018 0.0107 0.0048 0.0068 0.0012 0.006 0.0079 0.0213 0.0011
Vacuole 0.0006 0.0007 0.0004 0.0009 0.0004 0.0004 0.0029 0.0013 0.001 0.0025 0.0013 0.0007 0.0012 0.001 0.0011 0.002 0.0018 0.0004
Vacuole Periphery 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.001 0.0002 0.0002 0.0009 0.0004 0.0003 0.0004 0.0002 0.0002 0.0008 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.5476 24.4426 25.1584 37.0454 29.1612 17.6306 32.0882 38.7411 33.9851 35.6383
Translational Efficiency 0.9684 1.2584 1.2134 0.7187 0.9115 1.8656 0.9551 0.9273 1.0325 1.0048

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
747 260 1759 1278 567 1951 645 887 1314 2211 2404 2165

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.90 712.36 861.37 813.18 670.47 691.97 907.72 824.29 658.21 694.37 873.81 817.73
Standard Deviation 70.37 85.06 98.27 115.27 73.81 87.39 100.83 134.30 72.66 87.37 101.07 123.55
Intensity Change Log 2 0.134610 0.408637 0.325579 0.045537 0.437075 0.297979 0.090033 0.423158 0.311619

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000769 0.000262 0.001520 0.000083 0.000460 0.000351 0.001822 0.000113 0.000496 0.000286 0.001644
Bud Neck 0.016838 0.030423 0.006471 0.013919 0.005041 0.040055 0.003678 0.015120 0.011747 0.038922 0.005722 0.014411
Bud Site 0.003996 0.021093 0.009516 0.049181 0.002083 0.020901 0.009475 0.038013 0.003171 0.020923 0.009505 0.044606
Cell Periphery 0.000134 0.000206 0.000191 0.000334 0.000157 0.000155 0.000221 0.000594 0.000144 0.000161 0.000199 0.000441
Cytoplasm 0.458742* 0.363612* 0.514040* 0.490799* 0.535736* 0.437957* 0.373503* 0.466182* 0.491965* 0.429215* 0.476334* 0.480713*
Cytoplasmic Foci 0.288711* 0.306011* 0.014043 0.028067 0.214999* 0.256645* 0.023139 0.036588 0.256904* 0.262450* 0.016483 0.031558
Eisosomes 0.000186 0.000194 0.000040 0.000068 0.000121 0.000163 0.000089 0.000185 0.000158 0.000166 0.000053 0.000116
Endoplasmic Reticulum 0.000413 0.000747 0.002390 0.002969 0.000712 0.001109 0.003893 0.001082 0.000542 0.001066 0.002793 0.002196
Endosome 0.015026 0.038844 0.002078 0.006753 0.009328 0.023511 0.004834 0.007667 0.012567 0.025314 0.002818 0.007127
Golgi 0.002485 0.003741 0.000065 0.004568 0.000798 0.007667 0.000220 0.003814 0.001757 0.007205 0.000106 0.004259
Lipid Particles 0.007508 0.006575 0.001298 0.001584 0.006380 0.006266 0.002706 0.003248 0.007021 0.006302 0.001676 0.002265
Mitochondria 0.005814 0.010406 0.000607 0.002867 0.001090 0.013585 0.000822 0.004261 0.003775 0.013211 0.000665 0.003438
Mitotic Spindle 0.000262 0.001409 0.005424 0.027563 0.001007 0.008070 0.006745 0.027024 0.000584 0.007287 0.005778 0.027342
None 0.001834 0.002646 0.004096 0.006319 0.003504 0.001652 0.006569 0.011490 0.002555 0.001769 0.004759 0.008437
Nuclear Periphery 0.000316 0.000281 0.001732 0.001036 0.000376 0.000289 0.001845 0.000834 0.000342 0.000288 0.001763 0.000953
Nuclear Periphery Foci 0.000252 0.000469 0.001020 0.001997 0.000505 0.000314 0.002439 0.001129 0.000361 0.000333 0.001401 0.001641
Nucleolus 0.000833 0.002201 0.000264 0.000284 0.000781 0.001202 0.000759 0.001541 0.000811 0.001319 0.000397 0.000799
Nucleus 0.130654* 0.096481 0.371094* 0.187878* 0.171604* 0.089146 0.433282* 0.260347* 0.148324* 0.090009 0.387779* 0.217568*
Peroxisomes 0.006784 0.023654 0.000240 0.001396 0.002391 0.014113 0.000658 0.002738 0.004888 0.015235 0.000352 0.001946
Vacuole 0.058241 0.087528 0.064944 0.168934 0.042673 0.074168 0.123507 0.110519 0.051524 0.075739 0.080656 0.145001
Vacuole Periphery 0.000836 0.002711 0.000186 0.001967 0.000630 0.002572 0.001264 0.005803 0.000747 0.002589 0.000475 0.003538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.01 -5.91 -4.75 -0.76 -3.66 -4.30 -8.08 -1.82 -1.14 -1.16 -4.81 -9.70 -3.57 -1.97 -2.60
Bud Neck -2.93 5.85 4.09 4.88 -1.35 -16.50 1.62 -3.68 12.09 -4.56 -12.93 5.41 1.82 14.21 -3.30
Bud Site -5.20 -3.42 -7.49 -0.95 -5.76 -11.19 -3.22 -7.32 -1.24 -5.24 -11.54 -4.91 -10.45 -1.61 -7.73
Cell Periphery -1.84 -2.40 -2.70 0.14 -0.75 0.35 -2.77 -3.46 -3.53 -2.72 -1.57 -2.84 -4.34 -3.66 -2.69
Cytoplasm 5.08 2.59 5.83 -1.38 4.05 8.30 17.81 9.71 3.09 -8.27 6.77 11.24 10.73 4.25 -0.27
Cytoplasmic Foci -1.06 34.05 33.22 20.31 -4.07 -4.70 26.53 24.98 43.32 -4.42 -0.74 42.64 40.95 49.85 -6.69
Eisosomes -0.18 11.37 9.86 3.72 -4.50 -4.06 2.44 -0.21 1.22 -0.92 -0.67 12.44 4.94 5.30 -2.36
Endoplasmic Reticulum -2.37 -17.10 -18.21 -11.80 1.03 -1.07 -15.11 -8.02 -2.60 11.12 -1.68 -21.74 -19.18 -5.55 7.28
Endosome -4.03 7.83 7.00 6.34 -2.39 -8.18 3.84 3.23 13.86 -0.41 -7.74 9.00 7.65 16.83 -2.56
Golgi -1.27 5.85 1.49 2.20 -2.55 -8.54 5.67 -1.56 5.56 -2.53 -7.27 6.93 0.26 6.74 -3.62
Lipid Particles 0.68 9.81 9.40 3.51 -2.11 0.23 6.40 5.61 5.76 0.64 1.25 12.28 10.90 8.75 -0.98
Mitochondria -1.30 3.17 2.68 2.98 -2.43 -10.19 1.88 -1.86 8.33 -2.69 -6.48 3.36 2.04 9.84 -3.59
Mitotic Spindle -3.19 -5.00 -6.87 -6.44 -4.52 -3.18 -1.01 -5.43 -3.71 -4.76 -4.63 -4.01 -8.69 -5.30 -6.40
None -0.53 -3.46 -3.05 -1.57 -1.32 1.85 -1.19 -2.02 -3.58 -1.40 1.63 -2.48 -3.35 -4.90 -2.16
Nuclear Periphery 0.76 -28.18 -22.14 -20.61 5.24 2.08 -21.18 -10.14 -10.71 5.95 1.60 -34.50 -22.16 -21.46 7.80
Nuclear Periphery Foci -2.86 -10.30 -12.01 -9.44 -4.10 2.19 -8.11 -5.83 -6.98 4.41 0.45 -12.31 -13.00 -12.08 -0.47
Nucleolus -1.37 3.88 3.54 1.91 -0.69 -3.27 0.63 -0.50 1.06 -0.78 -2.98 4.42 1.75 4.06 -1.43
Nucleus 3.09 -33.26 -12.31 -12.21 21.51 10.81 -22.52 -7.78 -19.54 15.42 11.21 -39.28 -14.12 -27.62 25.83
Peroxisomes -5.87 8.83 7.82 8.29 -2.16 -10.37 3.54 1.21 11.37 -2.14 -9.69 9.49 7.16 14.61 -3.16
Vacuole -4.04 -8.73 -27.48 -19.03 -21.71 -8.41 -16.25 -20.36 -17.10 -3.29 -7.67 -17.15 -34.09 -29.94 -20.24
Vacuole Periphery -2.71 7.05 0.67 2.82 -2.43 -5.95 0.12 -1.97 -0.20 -1.90 -6.25 3.79 -1.69 1.89 -2.66
Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (33%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dma1

Dma1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dma1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available