Standard name | |
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Human Ortholog | |
Description | Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK1 has a paralog, YCK2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 6 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 4 | – | 3 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 5 | 8 | 6 | 16 | 18 | – | 31 | 10 | 18 | 0 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 165 | 159 | 370 | 410 | 484 | 381 | 527 | 531 | – | 407 | 209 | 288 | 279 | 340 | 445 | 153 | 344 | 177 | 159 | 154 | 359 | 319 | 478 | 515 |
Endoplasmic Reticulum | 2 | 2 | 3 | 2 | 7 | 13 | 21 | 13 | – | 28 | 5 | 20 | 1 | 3 | 4 | 4 | 11 | 10 | 0 | 0 | 7 | 2 | 3 | 7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 3 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 3 |
Mitochondria | 1 | 1 | 3 | 7 | 1 | 14 | 33 | 30 | – | 79 | 74 | 84 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 3 | 0 | 2 | 2 |
Nucleus | 6 | 1 | 1 | 0 | 1 | 4 | 6 | 19 | – | 9 | 6 | 14 | 2 | 0 | 5 | 15 | 17 | 6 | 2 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | – | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 1 | 3 | 23 | 25 | 20 | 108 | 157 | – | 64 | 27 | 41 | 5 | 2 | 1 | 0 | 1 | 1 | 8 | 0 | 0 | 2 | 4 | 5 |
Unique Cell Count | 171 | 162 | 381 | 434 | 517 | 422 | 671 | 736 | 567 | 296 | 424 | 285 | 344 | 450 | 156 | 348 | 178 | 178 | 158 | 384 | 332 | 505 | 550 | |
Labelled Cell Count | 175 | 165 | 386 | 448 | 527 | 440 | 714 | 777 | 630 | 342 | 476 | 289 | 346 | 457 | 177 | 378 | 195 | 178 | 158 | 384 | 332 | 505 | 550 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.3 | 11.4 | 10.6 | 10.5 | 10.8 | 9.3 | 9.0 | 9.1 | – | 8.3 | 8.0 | 8.2 | 13.1 | 13.1 | 13.2 | 8.6 | 9.7 | 11.8 | 13.5 | 13.2 | 13.4 |
Std Deviation (1e-4) | 1.9 | 1.7 | 1.5 | 1.7 | 1.7 | 1.6 | 1.5 | 1.6 | – | 1.4 | 1.5 | 1.5 | 1.7 | 1.7 | 1.6 | 1.2 | 1.5 | 1.8 | 1.6 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.02 | -0.2 | -0.25 | -0.22 | – | -0.36 | -0.41 | -0.37 | 0.3 | 0.3 | 0.31 | -0.3 | -0.13 | 0.15 | 0.35 | 0.31 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.9 | 1.9 | – | 3.8 | 2.4 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.9 | -2.4 | -3.9 | -8.2 | -10.0 | – | -9.9 | -9.7 | -10.7 | 0.6 | 1.6 | 1.9 | 0.6 | 1.7 | 1.8 |
Endoplasmic Reticulum | 0 | 0 | 2.3 | 2.4 | 1.3 | – | 3.5 | 0 | 3.3 | 0 | 0 | 0 | 0 | 2.3 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.5 | 3.5 | 3.1 | – | 7.1 | 9.8 | 8.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2.8 | – | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 4.0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.7 | 3.4 | 3.4 | 7.8 | 9.3 | – | 6.2 | 5.2 | 5.5 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.5798 | 11.879 | 11.4279 | 10.8334 | 11.3524 | 11.174 | 7.3507 | 11.2186 | 9.8777 | 10.1919 | 9.767 | 10.5934 | 6.8329 | 10.3579 | 9.2141 | 9.3337 | 7.7721 | 9.1002 |
Actin | 0.0032 | 0.0047 | 0.0099 | 0.0003 | 0.0045 | 0.0046 | 0.0293 | 0.002 | 0.003 | 0.0425 | 0.0066 | 0.0009 | 0.0795 | 0.0005 | 0.0009 | 0.0433 | 0.0019 | 0.0192 |
Bud | 0.0116 | 0.0105 | 0.0173 | 0.0001 | 0.0045 | 0.0093 | 0.0003 | 0.0001 | 0.0003 | 0.001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0002 |
Bud Neck | 0.0007 | 0.0003 | 0.0005 | 0.0006 | 0.0005 | 0.0018 | 0.0006 | 0.0002 | 0.0004 | 0.0008 | 0.0022 | 0.001 | 0.0006 | 0.0002 | 0.0014 | 0.0004 | 0.0005 | 0.001 |
Bud Periphery | 0.0025 | 0.0027 | 0.0058 | 0 | 0.0014 | 0.0022 | 0.0005 | 0 | 0.0002 | 0.0009 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0007 | 0.0002 |
Bud Site | 0.0045 | 0.005 | 0.0057 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0006 | 0.0044 | 0.0003 | 0 | 0.0009 | 0.0001 | 0.0019 | 0.0009 | 0.0004 | 0.0005 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.867 | 0.9493 | 0.9251 | 0.9819 | 0.9429 | 0.9346 | 0.8074 | 0.9894 | 0.9412 | 0.8726 | 0.9643 | 0.9885 | 0.759 | 0.9946 | 0.9468 | 0.8984 | 0.8701 | 0.9486 |
Cytoplasmic Foci | 0.0157 | 0.0032 | 0.0075 | 0.0014 | 0.0087 | 0.0086 | 0.0298 | 0.0012 | 0.0114 | 0.0091 | 0.0088 | 0.0018 | 0.0135 | 0.0005 | 0.0167 | 0.011 | 0.0029 | 0.004 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 | 0.0008 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0058 | 0.0013 | 0.0014 | 0.0011 | 0.0024 | 0.0026 | 0.0023 | 0.0003 | 0.0017 | 0.0007 | 0.0026 | 0.0012 | 0.0024 | 0.0013 | 0.0007 | 0.0024 | 0.0018 | 0.0006 |
Endosome | 0.0085 | 0.0015 | 0.0006 | 0.0004 | 0.0024 | 0.0015 | 0.0232 | 0.0002 | 0.0082 | 0.0034 | 0.0028 | 0.0003 | 0.0283 | 0.0001 | 0.0138 | 0.0032 | 0.0578 | 0.0024 |
Golgi | 0.0006 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0103 | 0.0001 | 0.0037 | 0.0046 | 0.0004 | 0.0001 | 0.0177 | 0 | 0.0034 | 0.0028 | 0.0204 | 0.0078 |
Lipid Particles | 0.0011 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.015 | 0 | 0.0032 | 0.0056 | 0.0001 | 0 | 0.0104 | 0 | 0.0018 | 0.0019 | 0.0015 | 0.0019 |
Mitochondria | 0.0111 | 0.0034 | 0.0012 | 0.0001 | 0.0011 | 0.0003 | 0.0036 | 0.0001 | 0.0035 | 0.0045 | 0.0002 | 0.0005 | 0.0123 | 0 | 0.0012 | 0.0007 | 0.0289 | 0.0039 |
None | 0.0168 | 0.0038 | 0.0044 | 0.0011 | 0.0049 | 0.0042 | 0.0326 | 0.0008 | 0.0018 | 0.0016 | 0.0034 | 0.0009 | 0.0175 | 0.0011 | 0.0012 | 0.0247 | 0.0006 | 0.0007 |
Nuclear Periphery | 0.0072 | 0.0016 | 0.0025 | 0.0011 | 0.0037 | 0.0028 | 0.0046 | 0.0003 | 0.0006 | 0.0009 | 0.0012 | 0.0005 | 0.0073 | 0.0003 | 0.0008 | 0.001 | 0.0012 | 0.0005 |
Nucleolus | 0.0054 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0 | 0.0012 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0266 | 0.0092 | 0.0123 | 0.0112 | 0.0161 | 0.0197 | 0.0034 | 0.0043 | 0.0021 | 0.0391 | 0.0036 | 0.0036 | 0.0042 | 0.0011 | 0.0026 | 0.0039 | 0.0028 | 0.0032 |
Peroxisomes | 0.0016 | 0.0003 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0032 | 0.0002 | 0.0166 | 0.0068 | 0.0007 | 0.0001 | 0.0037 | 0 | 0.0039 | 0.002 | 0.0003 | 0.0046 |
Punctate Nuclear | 0.0076 | 0.0016 | 0.0037 | 0.0004 | 0.0046 | 0.0056 | 0.0313 | 0.0002 | 0.0004 | 0.0006 | 0.0022 | 0.0002 | 0.0383 | 0.0001 | 0.0005 | 0.0015 | 0.0002 | 0.0003 |
Vacuole | 0.0016 | 0.0005 | 0.0006 | 0.0002 | 0.0007 | 0.0008 | 0.0008 | 0.0001 | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0014 | 0.0002 | 0.0041 | 0.0001 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0008 | 0 | 0.0003 | 0.0001 | 0.0033 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.5195 | 57.9504 | 47.5282 | 60.9991 | 48.6603 | 42.966 | 70.4665 | 71.1841 | 63.8848 | 61.855 |
Translational Efficiency | 1.3713 | 1.4443 | 1.1818 | 0.849 | 1.16 | 1.5518 | 1.0576 | 0.8812 | 0.9828 | 0.8228 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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