Standard name
Human Ortholog
Description Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK1 has a paralog, YCK2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.94 0.94 0.9 0.79 0.72 0.72 0.71 0.68 0.98 0.99 0.99 0.98 0.99 0.99 0.9 0.98 0.94 0.96 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.14 0.25 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.05 0.05 0.16 0.21 0.11 0.09 0.1 0 0 0 0 0 0 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 6 1 2 0 0 0 0 0 0 0 0 1 0 1 1
Bud 0 1 0 1 0 1 0 4 3 6 5 0 0 0 0 0 0 0 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 0 3 5 8 6 16 18 31 10 18 0 0 1 3 2 0 0 0 0 0 0 0
Cytoplasm 165 159 370 410 484 381 527 531 407 209 288 279 340 445 153 344 177 159 154 359 319 478 515
Endoplasmic Reticulum 2 2 3 2 7 13 21 13 28 5 20 1 3 4 4 11 10 0 0 7 2 3 7
Endosome 0 0 0 0 0 0 0 1 1 1 0 2 1 0 0 0 0 1 0 1 1 3 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 2 1 1 3
Mitochondria 1 1 3 7 1 14 33 30 79 74 84 0 0 0 2 2 1 0 0 3 0 2 2
Nucleus 6 1 1 0 1 4 6 19 9 6 14 2 0 5 15 17 6 2 0 0 0 1 0
Nuclear Periphery 0 0 1 0 0 1 1 1 1 2 3 0 0 0 0 1 0 0 0 1 0 1 0
Nucleolus 0 0 1 0 1 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 3 23 25 20 108 157 64 27 41 5 2 1 0 1 1 8 0 0 2 4 5
Unique Cell Count 171 162 381 434 517 422 671 736 567 296 424 285 344 450 156 348 178 178 158 384 332 505 550
Labelled Cell Count 175 165 386 448 527 440 714 777 630 342 476 289 346 457 177 378 195 178 158 384 332 505 550


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 11.4 10.6 10.5 10.8 9.3 9.0 9.1 8.3 8.0 8.2 13.1 13.1 13.2 8.6 9.7 11.8 13.5 13.2 13.4
Std Deviation (1e-4) 1.9 1.7 1.5 1.7 1.7 1.6 1.5 1.6 1.4 1.5 1.5 1.7 1.7 1.6 1.2 1.5 1.8 1.6 1.8 1.9
Intensity Change (Log2) -0.01 0.02 -0.2 -0.25 -0.22 -0.36 -0.41 -0.37 0.3 0.3 0.31 -0.3 -0.13 0.15 0.35 0.31 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.9 1.9 3.8 2.4 3.1 0 0 0 0 0 0
Cytoplasm -1.9 -2.4 -3.9 -8.2 -10.0 -9.9 -9.7 -10.7 0.6 1.6 1.9 0.6 1.7 1.8
Endoplasmic Reticulum 0 0 2.3 2.4 1.3 3.5 0 3.3 0 0 0 0 2.3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.5 3.5 3.1 7.1 9.8 8.7 0 0 0 0 0 0
Nucleus 0 0 0 0 2.8 0 0 3.2 0 0 0 0 4.0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.7 3.4 3.4 7.8 9.3 6.2 5.2 5.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5798 11.879 11.4279 10.8334 11.3524 11.174 7.3507 11.2186 9.8777 10.1919 9.767 10.5934 6.8329 10.3579 9.2141 9.3337 7.7721 9.1002
Actin 0.0032 0.0047 0.0099 0.0003 0.0045 0.0046 0.0293 0.002 0.003 0.0425 0.0066 0.0009 0.0795 0.0005 0.0009 0.0433 0.0019 0.0192
Bud 0.0116 0.0105 0.0173 0.0001 0.0045 0.0093 0.0003 0.0001 0.0003 0.001 0.0001 0.0002 0.0003 0.0001 0.0002 0.0002 0.0005 0.0002
Bud Neck 0.0007 0.0003 0.0005 0.0006 0.0005 0.0018 0.0006 0.0002 0.0004 0.0008 0.0022 0.001 0.0006 0.0002 0.0014 0.0004 0.0005 0.001
Bud Periphery 0.0025 0.0027 0.0058 0 0.0014 0.0022 0.0005 0 0.0002 0.0009 0 0.0001 0.0004 0 0.0001 0.0002 0.0007 0.0002
Bud Site 0.0045 0.005 0.0057 0.0001 0.0005 0.0005 0.0003 0.0005 0.0006 0.0044 0.0003 0 0.0009 0.0001 0.0019 0.0009 0.0004 0.0005
Cell Periphery 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.867 0.9493 0.9251 0.9819 0.9429 0.9346 0.8074 0.9894 0.9412 0.8726 0.9643 0.9885 0.759 0.9946 0.9468 0.8984 0.8701 0.9486
Cytoplasmic Foci 0.0157 0.0032 0.0075 0.0014 0.0087 0.0086 0.0298 0.0012 0.0114 0.0091 0.0088 0.0018 0.0135 0.0005 0.0167 0.011 0.0029 0.004
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0 0.0002 0 0 0.0004 0 0 0.0008 0 0.0001
Endoplasmic Reticulum 0.0058 0.0013 0.0014 0.0011 0.0024 0.0026 0.0023 0.0003 0.0017 0.0007 0.0026 0.0012 0.0024 0.0013 0.0007 0.0024 0.0018 0.0006
Endosome 0.0085 0.0015 0.0006 0.0004 0.0024 0.0015 0.0232 0.0002 0.0082 0.0034 0.0028 0.0003 0.0283 0.0001 0.0138 0.0032 0.0578 0.0024
Golgi 0.0006 0.0004 0.0002 0 0.0002 0.0001 0.0103 0.0001 0.0037 0.0046 0.0004 0.0001 0.0177 0 0.0034 0.0028 0.0204 0.0078
Lipid Particles 0.0011 0.0002 0.0002 0 0.0001 0.0001 0.015 0 0.0032 0.0056 0.0001 0 0.0104 0 0.0018 0.0019 0.0015 0.0019
Mitochondria 0.0111 0.0034 0.0012 0.0001 0.0011 0.0003 0.0036 0.0001 0.0035 0.0045 0.0002 0.0005 0.0123 0 0.0012 0.0007 0.0289 0.0039
None 0.0168 0.0038 0.0044 0.0011 0.0049 0.0042 0.0326 0.0008 0.0018 0.0016 0.0034 0.0009 0.0175 0.0011 0.0012 0.0247 0.0006 0.0007
Nuclear Periphery 0.0072 0.0016 0.0025 0.0011 0.0037 0.0028 0.0046 0.0003 0.0006 0.0009 0.0012 0.0005 0.0073 0.0003 0.0008 0.001 0.0012 0.0005
Nucleolus 0.0054 0.0001 0.0002 0 0.0002 0.0002 0.0007 0 0 0.0001 0 0 0.0012 0 0.0003 0.0001 0.0001 0
Nucleus 0.0266 0.0092 0.0123 0.0112 0.0161 0.0197 0.0034 0.0043 0.0021 0.0391 0.0036 0.0036 0.0042 0.0011 0.0026 0.0039 0.0028 0.0032
Peroxisomes 0.0016 0.0003 0.0005 0 0.0003 0.0002 0.0032 0.0002 0.0166 0.0068 0.0007 0.0001 0.0037 0 0.0039 0.002 0.0003 0.0046
Punctate Nuclear 0.0076 0.0016 0.0037 0.0004 0.0046 0.0056 0.0313 0.0002 0.0004 0.0006 0.0022 0.0002 0.0383 0.0001 0.0005 0.0015 0.0002 0.0003
Vacuole 0.0016 0.0005 0.0006 0.0002 0.0007 0.0008 0.0008 0.0001 0.0006 0.0005 0.0002 0.0001 0.0011 0.0001 0.0014 0.0002 0.0041 0.0001
Vacuole Periphery 0.0005 0.0001 0.0002 0 0.0002 0.0002 0.0005 0 0.0002 0.0002 0.0001 0 0.0008 0 0.0003 0.0001 0.0033 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.5195 57.9504 47.5282 60.9991 48.6603 42.966 70.4665 71.1841 63.8848 61.855
Translational Efficiency 1.3713 1.4443 1.1818 0.849 1.16 1.5518 1.0576 0.8812 0.9828 0.8228

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
83 191 859 466 645 1839 1425 1567 728 2030 2284 2033

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1237.79 1163.17 1305.31 1413.55 891.50 1088.73 1324.46 1264.58 930.98 1095.73 1317.26 1298.73
Standard Deviation 139.30 185.58 157.12 196.96 159.62 147.99 193.66 203.37 192.09 153.47 181.02 211.40
Intensity Change Log 2 -0.089705 0.076626 0.191556 0.288340 0.571098 0.504352 0.080770 0.304564 0.330854

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001140 0.000241 0.000331 0.000798 0.000533 0.000304 0.001603 0.001989 0.000603 0.000298 0.001125 0.001716
Bud Neck 0.002075 0.000785 0.000923 0.002235 0.001716 0.001149 0.003547 0.002817 0.001757 0.001115 0.002560 0.002683
Bud Site 0.036472 0.008382 0.003861 0.007425 0.018274 0.008610 0.015839 0.025400 0.020348 0.008588 0.011334 0.021280
Cell Periphery 0.000251 0.000086 0.000124 0.000278 0.000206 0.000058 0.000106 0.000071 0.000211 0.000061 0.000113 0.000119
Cytoplasm 0.385301* 0.833133* 0.757073* 0.711352* 0.721628* 0.838239* 0.783816* 0.783996* 0.683283* 0.837758* 0.773758* 0.767345*
Cytoplasmic Foci 0.171949 0.069733 0.011195 0.015732 0.154565 0.051924 0.012386 0.018804 0.156547 0.053599 0.011938 0.018100
Eisosomes 0.000059 0.000011 0.000005 0.000013 0.000038 0.000009 0.000008 0.000008 0.000040 0.000009 0.000007 0.000009
Endoplasmic Reticulum 0.001177 0.000309 0.000389 0.000921 0.001990 0.000460 0.000325 0.000227 0.001897 0.000446 0.000349 0.000386
Endosome 0.026065 0.001977 0.000626 0.003387 0.009048 0.003002 0.002621 0.003349 0.010988 0.002906 0.001871 0.003358
Golgi 0.011698 0.000399 0.000411 0.001915 0.003151 0.002449 0.003858 0.002861 0.004125 0.002256 0.002562 0.002644
Lipid Particles 0.001174 0.000455 0.000558 0.001587 0.002373 0.000449 0.001526 0.000608 0.002236 0.000450 0.001162 0.000832
Mitochondria 0.047348 0.000018 0.000132 0.001467 0.000301 0.001242 0.001179 0.003596 0.005665 0.001127 0.000785 0.003108
Mitotic Spindle 0.002940 0.000372 0.003273 0.006743 0.001068 0.006436 0.027332 0.025459 0.001282 0.005866 0.018284 0.021169
None 0.000243 0.003671 0.006849 0.005575 0.004805 0.006231 0.007991 0.006723 0.004285 0.005990 0.007562 0.006460
Nuclear Periphery 0.000170 0.000056 0.000243 0.000375 0.000095 0.000062 0.000278 0.000146 0.000103 0.000061 0.000265 0.000198
Nuclear Periphery Foci 0.005164 0.005081 0.003632 0.006802 0.004431 0.002215 0.001855 0.001728 0.004515 0.002485 0.002523 0.002891
Nucleolus 0.000066 0.000090 0.000088 0.000123 0.000450 0.000134 0.000891 0.000159 0.000406 0.000130 0.000589 0.000151
Nucleus 0.012606 0.029456 0.179917* 0.149500* 0.023372 0.041531 0.099230 0.079131 0.022144 0.040395 0.129576 0.095261
Peroxisomes 0.006012 0.000325 0.000047 0.000102 0.001165 0.000851 0.001133 0.002536 0.001718 0.000801 0.000725 0.001978
Vacuole 0.261144* 0.045297 0.030061 0.082075 0.049886 0.034009 0.031591 0.038633 0.073972 0.035071 0.031016 0.048591
Vacuole Periphery 0.026946 0.000121 0.000260 0.001593 0.000905 0.000636 0.002883 0.001760 0.003874 0.000588 0.001897 0.001722

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.22 2.33 0.04 -3.69 -2.65 2.60 -1.13 -1.39 -2.39 -0.09 3.33 -0.63 -1.53 -3.03 -0.76
Bud Neck 3.71 0.02 -4.12 -5.74 -4.18 2.54 -1.93 -0.33 -2.35 1.40 2.87 -1.54 -2.35 -4.30 -0.89
Bud Site 3.02 3.80 2.71 -1.06 -4.53 5.46 5.11 2.85 -2.35 -2.02 6.18 7.12 3.82 -2.56 -3.52
Cell Periphery 2.75 -0.11 -3.54 -7.43 -4.48 9.93 4.47 8.16 -5.57 4.66 10.33 3.97 4.98 -9.72 1.45
Cytoplasm -13.00 -10.06 -5.38 14.60 10.16 -11.25 0.21 1.15 17.16 1.23 -14.32 -2.67 0.75 23.07 5.20
Cytoplasmic Foci 0.26 4.65 4.17 7.03 -3.43 15.74 22.85 22.39 16.16 -2.01 15.03 22.95 22.31 17.34 -3.12
Eisosomes 6.71 7.61 5.26 -4.21 -9.34 9.43 9.56 9.03 -1.74 -2.29 10.70 11.04 9.54 -4.69 -6.46
Endoplasmic Reticulum 2.58 1.43 0.06 -4.95 -2.74 2.74 3.08 3.17 2.52 1.27 2.83 3.02 3.06 1.20 0.56
Endosome 2.34 2.77 2.49 0.90 -1.93 4.97 6.51 6.49 4.37 -0.11 5.41 7.17 6.90 4.41 -0.93
Golgi 1.23 1.26 1.17 -0.36 -0.59 1.19 2.56 3.56 2.67 0.65 1.45 3.42 3.85 2.80 0.37
Lipid Particles 3.98 1.02 -5.43 -9.58 -7.28 3.38 1.24 2.35 -3.98 1.95 3.56 1.59 1.50 -8.04 -0.33
Mitochondria 1.07 1.06 1.04 -1.66 -1.01 -2.08 -0.86 -1.38 0.48 -1.00 0.42 1.01 0.65 0.59 -1.29
Mitotic Spindle 2.10 -3.50 -3.94 -4.69 -1.85 -3.05 -4.88 -4.58 -1.70 1.02 -3.00 -5.28 -5.30 -2.07 0.11
None -2.40 -13.40 -10.30 -0.54 4.58 -0.66 -0.35 -0.06 1.35 0.65 -0.89 -0.88 -0.22 1.52 1.89
Nuclear Periphery 7.14 -3.36 -7.43 -16.28 -5.77 1.20 -8.27 -7.38 -18.65 3.77 2.38 -10.13 -10.27 -23.44 1.66
Nuclear Periphery Foci -0.68 0.32 -1.36 -0.89 -2.17 4.97 5.64 5.92 1.47 0.00 4.57 4.13 3.18 -1.44 -0.82
Nucleolus -2.99 -5.00 -5.99 -3.58 -1.70 2.10 0.45 1.98 -0.99 1.00 2.06 0.83 1.91 -1.34 0.92
Nucleus -1.24 -26.92 -15.68 -15.15 7.39 -7.96 -27.79 -22.84 -19.70 8.49 -8.13 -37.82 -26.97 -24.10 13.07
Peroxisomes 2.30 2.86 2.75 2.36 -4.10 1.56 3.52 1.43 0.08 -1.51 2.13 4.92 2.52 0.56 -1.90
Vacuole 11.69 11.30 5.72 -13.68 -13.60 2.68 -9.67 -15.88 -19.57 -6.79 7.80 0.49 -8.80 -24.63 -13.40
Vacuole Periphery 1.01 1.01 0.98 -0.90 -0.66 0.97 0.20 1.28 0.63 0.97 1.29 1.23 1.45 0.67 0.60
Standard name
Human Ortholog
Description Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK1 has a paralog, YCK2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yck1

Yck1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yck1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available