Standard name
Human Ortholog
Description Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0.06 0.07 0.09 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 1.0 0.96 0.97 0.92 0.89 0.87 0.86 0.88 0.92 0.99 0.99 1.0 1.0 0.99 1.0 0.93 0.94 0.97 0.92 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.08 0.08 0.11 0.07 0.06 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 2 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 1 0 4 4 1 4 2 3 6 2 1 0 0 0 0 0 1 2 1 0 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 19 4 6 12 29 34 38 20 15 21 7 5 2 4 1 1 0 0 0 0 0 0
Cytoplasm 37 145 351 229 306 361 443 439 363 393 319 336 207 249 258 209 138 117 52 136 330 27 168 219
Endoplasmic Reticulum 0 0 1 1 8 6 29 40 33 51 27 21 2 1 0 10 7 5 0 1 3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 1 0 0 0 0 0 0
Nucleus 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 4 0 0 1 0 0 1 1 0 1 0 0 3 0 0 2 1 0 1 2
Unique Cell Count 37 145 354 230 319 372 483 493 415 456 361 367 209 251 258 210 140 117 57 146 340 30 180 232
Labelled Cell Count 38 147 375 234 328 384 502 519 436 470 373 384 217 256 260 223 149 124 57 146 340 30 180 232


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.6 62.0 50.0 49.6 44.3 52.6 53.3 55.4 53.7 50.6 51.5 50.0 49.4 53.8 57.1 118.2 110.6 114.4 – 43.5 50.1
Std Deviation (1e-4) 15.3 18.5 11.3 13.7 15.3 13.2 13.0 13.6 14.1 12.7 13.2 13.1 12.8 17.2 15.9 39.2 48.3 39.8 – 15.2 15.6
Intensity Change (Log2) – – – -0.01 -0.18 0.07 0.09 0.15 0.1 0.02 0.04 -0.0 -0.02 0.11 0.19 1.24 1.14 1.19 – -0.2 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.2 -2.4 -1.4 0.4 0.9 2.0 -0.6 -0.8 0.2 -1.1 -2.1 -3.1 -2.0 -2.4 0
Cytoplasm 0.6 -2.7 -2.1 -4.8 -5.8 -6.3 -6.7 -5.9 -4.8 -0.1 0.1 1.5 0.5 -0.6 1.0
Endoplasmic Reticulum 0 0 0 4.4 5.2 5.2 6.3 5.0 4.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.7322 52.0125 49.1534 45.1801 40.4271 48.6503 28.3489 42.5754 40.0185 39.7163 32.2887 41.7364 34.5985 46.6664 43.2686 43.3988 37.7134 43.2925
Actin 0.0079 0.0001 0.001 0 0.0014 0.0001 0.016 0.0001 0.0009 0.0004 0.0006 0.0001 0.0114 0.0003 0.0081 0.0007 0.0014 0.0039
Bud 0.0007 0.0001 0.0022 0 0.0002 0 0.0008 0 0.0006 0 0.0007 0.0001 0.0014 0.0005 0.0007 0.0006 0.0005 0.0004
Bud Neck 0.0003 0.0001 0.0003 0.0002 0.0004 0.0005 0.001 0.0001 0.0002 0.0001 0.0002 0.0004 0.0007 0.0001 0.0003 0.0005 0.0003 0.0005
Bud Periphery 0.0007 0 0.0008 0 0.0002 0 0.0006 0 0.0003 0 0.0005 0 0.0008 0.0001 0.0005 0.0006 0.001 0.0003
Bud Site 0.0004 0 0.0028 0 0.0002 0 0.0033 0 0.0005 0.0001 0.0002 0 0.0012 0.0005 0.0009 0.0004 0.0004 0.0006
Cell Periphery 0.0004 0 0.0001 0 0.0007 0 0.0002 0 0.0001 0 0.0001 0 0.0004 0 0.0001 0.0004 0.0007 0.0001
Cytoplasm 0.9283 0.9973 0.9882 0.9967 0.834 0.9935 0.8756 0.9976 0.9505 0.9539 0.9115 0.9692 0.9136 0.9897 0.9513 0.9271 0.9171 0.9678
Cytoplasmic Foci 0.0057 0.0002 0.0005 0.0002 0.0184 0.0007 0.0189 0.0004 0.0097 0.0242 0.0048 0.0151 0.0133 0.0008 0.0027 0.0016 0.0056 0.0097
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0021 0.0002 0.0003 0.0001 0.0062 0.0007 0.0049 0.0001 0.0019 0.0001 0.0009 0.0003 0.0035 0.0001 0.0018 0.001 0.0029 0.0005
Endosome 0.0061 0.0001 0.0002 0.0001 0.019 0.0001 0.0163 0 0.0074 0.0005 0.0191 0.0015 0.0113 0.0002 0.0058 0.0024 0.0091 0.0012
Golgi 0.0014 0 0.0001 0 0.0019 0 0.0058 0 0.0013 0.0006 0.0037 0.0002 0.0022 0.0001 0.0011 0.0009 0.0046 0.0011
Lipid Particles 0.0037 0 0 0 0.0151 0 0.0045 0 0.0061 0.0119 0.0008 0.0018 0.0064 0.0004 0.001 0.0075 0.0232 0.0003
Mitochondria 0.0032 0 0.0001 0 0.0163 0 0.0037 0 0.0037 0.0003 0.0348 0.0029 0.0068 0.001 0.0102 0.0326 0.0086 0.001
None 0.0031 0.0002 0.0003 0.0001 0.0029 0.0002 0.0031 0.0003 0.0007 0.0002 0.0004 0.0004 0.0021 0.0002 0.0005 0.0005 0.0007 0.0009
Nuclear Periphery 0.0053 0.0003 0.0006 0.0004 0.016 0.0007 0.0135 0.0002 0.0035 0.0003 0.0012 0.0012 0.0042 0.0004 0.0035 0.0021 0.0009 0.0008
Nucleolus 0.0041 0 0 0 0.0032 0 0.0004 0 0.0003 0 0.0001 0.0002 0.0009 0 0.0005 0.0013 0.0002 0
Nucleus 0.011 0.0011 0.0019 0.0018 0.0055 0.0029 0.0102 0.0011 0.0039 0.0019 0.0028 0.0041 0.0072 0.0016 0.0027 0.0068 0.0025 0.0053
Peroxisomes 0.0028 0 0 0 0.0009 0 0.0088 0 0.0007 0.0048 0.0004 0.0007 0.0006 0.0034 0.0006 0.0019 0.0098 0.0047
Punctate Nuclear 0.0038 0 0.0002 0 0.0033 0.0001 0.0031 0.0001 0.0008 0.0006 0.0001 0.0002 0.0043 0.0001 0.0002 0.0003 0.0002 0.0002
Vacuole 0.0067 0.0002 0.0003 0.0002 0.027 0.0003 0.0063 0.0001 0.0049 0 0.0098 0.001 0.0049 0.0004 0.0053 0.0084 0.0085 0.0005
Vacuole Periphery 0.0025 0 0.0001 0 0.0272 0.0001 0.0027 0 0.0023 0 0.0076 0.0006 0.0026 0.0001 0.0019 0.0024 0.0017 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 495.7524 509.9967 495.8653 484.2017 434.654 478.5189 622.8093 605.7019 608.5388 523.3298
Translational Efficiency 1.7779 2.1037 1.8995 1.8523 1.9354 2.0183 1.8713 1.8109 1.756 1.9551

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Oye2

Oye2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oye2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available