Standard name | |
---|---|
Human Ortholog | |
Description | ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 36 | 29 | 54 | 79 | 108 | 211 | 266 | 201 | 183 | 233 | 264 | 113 | 113 | 80 | 74 | 51 | 25 | 0 | 2 | 0 | 1 | 7 | 6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 1 | 0 | 2 | 3 | 0 | 0 | 10 | 11 | 9 | 0 | 0 | 0 | 0 | 3 | 2 |
Endosome | 5 | 0 | 7 | 10 | 16 | 7 | 7 | 2 | 1 | 0 | 0 | 1 | 7 | 6 | 1 | 4 | 6 | 7 | 16 | 12 | 7 | 12 | 19 | 27 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 2 | 0 | 3 |
Mitochondria | 27 | 67 | 47 | 10 | 16 | 35 | 33 | 44 | 52 | 52 | 55 | 55 | 0 | 0 | 0 | 17 | 15 | 8 | 12 | 6 | 8 | 5 | 12 | 15 |
Nucleus | 70 | 32 | 15 | 13 | 9 | 17 | 26 | 32 | 16 | 16 | 12 | 22 | 5 | 5 | 3 | 87 | 62 | 43 | 7 | 2 | 0 | 1 | 4 | 2 |
Nuclear Periphery | 2 | 2 | 0 | 0 | 1 | 0 | 4 | 5 | 8 | 3 | 2 | 6 | 0 | 0 | 0 | 2 | 0 | 0 | 6 | 0 | 0 | 1 | 4 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 |
Vac/Vac Membrane | 260 | 231 | 164 | 146 | 221 | 213 | 309 | 317 | 212 | 172 | 164 | 203 | 85 | 72 | 19 | 93 | 81 | 89 | 320 | 275 | 206 | 208 | 328 | 293 |
Unique Cell Count | 333 | 290 | 217 | 180 | 275 | 292 | 434 | 514 | 371 | 321 | 362 | 428 | 191 | 183 | 98 | 209 | 173 | 138 | 369 | 305 | 231 | 239 | 391 | 367 |
Labelled Cell Count | 395 | 368 | 263 | 233 | 345 | 383 | 597 | 667 | 490 | 429 | 468 | 556 | 218 | 202 | 106 | 288 | 226 | 186 | 369 | 305 | 231 | 239 | 391 | 367 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 5.6 | 5.7 | 6.4 | 6.8 | 5.7 | 5.6 | 5.6 | 5.1 | 4.8 | 4.9 | 5.1 | 6.2 | 6.2 | 5.9 | 5.1 | 5.8 | 6.8 | 7.0 | 6.7 | 7.1 |
Std Deviation (1e-4) | 1.3 | 1.2 | 1.6 | 1.8 | 1.7 | 1.6 | 1.5 | 1.6 | 1.3 | 1.1 | 1.4 | 1.2 | 1.4 | 1.4 | 1.7 | 1.1 | 1.4 | 1.6 | 1.7 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.26 | 0.01 | -0.01 | -0.02 | -0.15 | -0.24 | -0.2 | -0.16 | 0.13 | 0.13 | 0.06 | -0.15 | 0.03 | 0.27 | 0.3 | 0.25 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.1 | 4.1 | 5.9 | 8.8 | 9.7 | 9.8 | 10.2 | 11.9 | 11.6 | 9.7 | 10.1 | 11.8 | 5.3 | 3.9 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.1 | 1.4 | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | -0.9 | 0.1 | 0.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -4.6 | -5.2 | -2.9 | -5.1 | -4.9 | -2.4 | 0 | -2.0 | -2.9 | -6.8 | -6.7 | -5.0 | -3.9 | -3.5 | -4.0 |
Nucleus | 0.1 | -1.9 | -0.5 | -0.5 | -0.3 | -1.4 | -0.9 | -2.0 | -0.9 | -2.0 | -1.9 | -1.4 | 8.4 | 7.1 | 6.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.3 | 1.3 | -0.7 | -1.2 | -3.6 | -4.5 | -5.2 | -7.1 | -6.8 | -6.4 | -7.3 | -9.4 | -6.6 | -5.8 | -2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6476 | 2.7373 | 2.0418 | 1.9911 | 1.6676 | 2.2762 | 2.4594 | 3.6835 | 3.1717 | 2.9013 | 3.1141 | 3.4375 | 2.6661 | 3.7614 | 3.1885 | 3.5936 | 2.9123 | 3.4412 |
Actin | 0.0169 | 0.0053 | 0.0098 | 0 | 0.0036 | 0.0007 | 0.0188 | 0 | 0.0021 | 0.0086 | 0.0561 | 0.0004 | 0.0095 | 0 | 0.0056 | 0 | 0.0102 | 0.0002 |
Bud | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0006 | 0.0018 | 0.0031 | 0.0003 | 0.0005 | 0.0008 | 0.0009 | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0007 |
Bud Neck | 0.0016 | 0.0011 | 0.002 | 0.0007 | 0.0012 | 0.0008 | 0.0011 | 0.0008 | 0.0009 | 0.0106 | 0.0012 | 0.001 | 0.0016 | 0.0014 | 0.001 | 0.0004 | 0.0009 | 0.0012 |
Bud Periphery | 0.0013 | 0.0008 | 0.0009 | 0.0006 | 0.0009 | 0.0009 | 0.0036 | 0.0005 | 0.0012 | 0.0015 | 0.0021 | 0.0006 | 0.0009 | 0.0006 | 0.001 | 0.0003 | 0.0008 | 0.0015 |
Bud Site | 0.0021 | 0.0019 | 0.0021 | 0.0009 | 0.0016 | 0.0005 | 0.0053 | 0.0015 | 0.0014 | 0.0073 | 0.0019 | 0.0006 | 0.0016 | 0.001 | 0.001 | 0.0005 | 0.0016 | 0.0014 |
Cell Periphery | 0.0011 | 0.0009 | 0.001 | 0.0006 | 0.0008 | 0.001 | 0.0009 | 0.0012 | 0.0012 | 0.0026 | 0.0006 | 0.0009 | 0.0017 | 0.0132 | 0.0011 | 0.0006 | 0.0006 | 0.0011 |
Cytoplasm | 0.0161 | 0.0253 | 0.0106 | 0.0048 | 0.01 | 0.0118 | 0.0091 | 0.0194 | 0.0161 | 0.0038 | 0.0031 | 0.0062 | 0.0126 | 0.0148 | 0.0164 | 0.0037 | 0.0165 | 0.0141 |
Cytoplasmic Foci | 0.0186 | 0.0086 | 0.0236 | 0.0086 | 0.0156 | 0.0076 | 0.0171 | 0.0168 | 0.0148 | 0.0204 | 0.0391 | 0.01 | 0.0142 | 0.011 | 0.0143 | 0.0062 | 0.014 | 0.0078 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0057 | 0.0032 | 0.0037 | 0.0016 | 0.0052 | 0.0031 | 0.0043 | 0.006 | 0.0069 | 0.002 | 0.0048 | 0.0044 | 0.0053 | 0.0078 | 0.0062 | 0.0011 | 0.0035 | 0.0057 |
Endosome | 0.3194 | 0.2078 | 0.3173 | 0.3336 | 0.334 | 0.2216 | 0.3369 | 0.2589 | 0.3461 | 0.3719 | 0.381 | 0.3074 | 0.3343 | 0.2175 | 0.3149 | 0.3079 | 0.2662 | 0.2709 |
Golgi | 0.014 | 0.0049 | 0.0157 | 0.009 | 0.0183 | 0.0103 | 0.0172 | 0.0058 | 0.0195 | 0.0254 | 0.0203 | 0.0118 | 0.0109 | 0.0055 | 0.01 | 0.0048 | 0.0083 | 0.007 |
Lipid Particles | 0.0104 | 0.0062 | 0.0156 | 0.0067 | 0.0103 | 0.0205 | 0.0104 | 0.0074 | 0.0056 | 0.0192 | 0.0183 | 0.0059 | 0.0197 | 0.0036 | 0.0049 | 0.0038 | 0.0123 | 0.0118 |
Mitochondria | 0.0218 | 0.008 | 0.0175 | 0.0325 | 0.0081 | 0.0184 | 0.038 | 0.004 | 0.0291 | 0.0136 | 0.021 | 0.0102 | 0.0298 | 0.0041 | 0.0284 | 0.002 | 0.0071 | 0.0052 |
None | 0.014 | 0.0043 | 0.013 | 0.0002 | 0.0019 | 0.0035 | 0.0042 | 0.0045 | 0.023 | 0.0011 | 0.0004 | 0.0028 | 0.0095 | 0.001 | 0.0175 | 0.0001 | 0.0027 | 0.0004 |
Nuclear Periphery | 0.0252 | 0.0076 | 0.0071 | 0.0031 | 0.0168 | 0.0061 | 0.0115 | 0.0238 | 0.0095 | 0.0025 | 0.0038 | 0.0068 | 0.0052 | 0.0082 | 0.0073 | 0.0025 | 0.0128 | 0.0063 |
Nucleolus | 0.0051 | 0.0014 | 0.0029 | 0.0018 | 0.0015 | 0.0013 | 0.0012 | 0.0022 | 0.002 | 0.0054 | 0.0008 | 0.001 | 0.0062 | 0.0009 | 0.0012 | 0.0007 | 0.0015 | 0.0016 |
Nucleus | 0.0084 | 0.0037 | 0.0035 | 0.0018 | 0.0063 | 0.0029 | 0.0036 | 0.0038 | 0.0064 | 0.0022 | 0.002 | 0.0018 | 0.0059 | 0.0056 | 0.0024 | 0.0016 | 0.0049 | 0.0075 |
Peroxisomes | 0.0065 | 0.0007 | 0.0038 | 0.0009 | 0.0026 | 0.0026 | 0.0045 | 0.0005 | 0.001 | 0.0048 | 0.0729 | 0.0009 | 0.0023 | 0.0007 | 0.0011 | 0.0003 | 0.0112 | 0.0029 |
Punctate Nuclear | 0.0011 | 0.0003 | 0.0009 | 0.0001 | 0.0009 | 0.0004 | 0.001 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0013 | 0.0002 | 0.0002 | 0 | 0.0013 | 0.0001 |
Vacuole | 0.3878 | 0.5646 | 0.4311 | 0.4862 | 0.4708 | 0.5454 | 0.3953 | 0.4898 | 0.3881 | 0.4013 | 0.2996 | 0.4569 | 0.4152 | 0.5802 | 0.4228 | 0.5461 | 0.5231 | 0.5376 |
Vacuole Periphery | 0.1218 | 0.1429 | 0.1175 | 0.106 | 0.089 | 0.1388 | 0.1126 | 0.1525 | 0.1239 | 0.0947 | 0.0694 | 0.1701 | 0.1108 | 0.1222 | 0.1424 | 0.1171 | 0.1 | 0.115 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.1967 | 24.8233 | 20.1577 | 30.1724 | 22.417 | 30.895 | 26.1644 | 25.41 | 28.8634 | 30.7797 |
Translational Efficiency | 1.2312 | 0.9043 | 1.3705 | 0.9627 | 1.021 | 1.0382 | 1.1344 | 1.1439 | 0.8937 | 0.9075 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
171 | 439 | 287 | 1226 | 1963 | 688 | 191 | 1526 | 2134 | 1127 | 478 | 2752 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 919.46 | 836.56 | 983.02 | 856.22 | 810.60 | 833.66 | 942.04 | 903.33 | 819.32 | 834.79 | 966.65 | 882.34 |
Standard Deviation | 127.52 | 125.73 | 106.46 | 121.79 | 119.70 | 113.30 | 99.35 | 122.89 | 123.92 | 118.31 | 105.60 | 124.62 |
Intensity Change Log 2 | — | -0.136318 | 0.096434 | -0.102805 | — | 0.040469 | 0.216798 | 0.156263 | — | -0.050784 | 0.154081 | 0.024384 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000708 | 0.000033 | 0.000834 | 0.001655 | 0.000006 | 0.000029 | 0.000308 | 0.001654 | 0.000062 | 0.000030 | 0.000624 | 0.001655 |
Bud Neck | 0.012869 | 0.010625 | 0.014010 | 0.021973 | 0.004401 | 0.013136 | 0.022414 | 0.019453 | 0.005079 | 0.012158 | 0.017368 | 0.020575 |
Bud Site | 0.022562 | 0.008734 | 0.026925 | 0.048007 | 0.003167 | 0.003650 | 0.013350 | 0.029794 | 0.004721 | 0.005630 | 0.021500 | 0.037908 |
Cell Periphery | 0.000515 | 0.000410 | 0.000119 | 0.000108 | 0.000825 | 0.001038 | 0.000114 | 0.000074 | 0.000800 | 0.000794 | 0.000117 | 0.000089 |
Cytoplasm | 0.112825* | 0.021110 | 0.169182* | 0.191366* | 0.007092 | 0.009532 | 0.238219* | 0.159443* | 0.015564 | 0.014042 | 0.196768* | 0.173665* |
Cytoplasmic Foci | 0.066472 | 0.016857 | 0.061448 | 0.073866 | 0.005478 | 0.009295 | 0.059373 | 0.063221 | 0.010365 | 0.012240 | 0.060619 | 0.067963 |
Eisosomes | 0.000071 | 0.000017 | 0.000065 | 0.000034 | 0.000006 | 0.000022 | 0.000070 | 0.000023 | 0.000011 | 0.000020 | 0.000067 | 0.000028 |
Endoplasmic Reticulum | 0.003742 | 0.001763 | 0.005504 | 0.001560 | 0.001939 | 0.002716 | 0.004585 | 0.001059 | 0.002084 | 0.002344 | 0.005136 | 0.001282 |
Endosome | 0.072613 | 0.070309 | 0.086713 | 0.118912 | 0.041314 | 0.058457 | 0.076016 | 0.170790 | 0.043822 | 0.063074 | 0.082439 | 0.147679 |
Golgi | 0.004405 | 0.002587 | 0.001225 | 0.004707 | 0.000636 | 0.000980 | 0.000267 | 0.007104 | 0.000938 | 0.001606 | 0.000842 | 0.006036 |
Lipid Particles | 0.002322 | 0.001878 | 0.007365 | 0.002741 | 0.000726 | 0.003372 | 0.005233 | 0.002226 | 0.000854 | 0.002790 | 0.006513 | 0.002455 |
Mitochondria | 0.005774 | 0.001097 | 0.000647 | 0.004832 | 0.001315 | 0.001846 | 0.000276 | 0.003584 | 0.001672 | 0.001555 | 0.000498 | 0.004140 |
Mitotic Spindle | 0.001897 | 0.001771 | 0.015750 | 0.023371 | 0.000116 | 0.000781 | 0.004465 | 0.031665 | 0.000259 | 0.001166 | 0.011241 | 0.027970 |
None | 0.000103 | 0.000221 | 0.000630 | 0.000744 | 0.000057 | 0.000087 | 0.001337 | 0.000792 | 0.000060 | 0.000139 | 0.000913 | 0.000771 |
Nuclear Periphery | 0.001637 | 0.000114 | 0.003271 | 0.001164 | 0.000106 | 0.000376 | 0.002451 | 0.001276 | 0.000228 | 0.000274 | 0.002943 | 0.001226 |
Nuclear Periphery Foci | 0.004453 | 0.000359 | 0.013148 | 0.001897 | 0.000073 | 0.000220 | 0.009156 | 0.001168 | 0.000424 | 0.000274 | 0.011553 | 0.001493 |
Nucleolus | 0.001252 | 0.000376 | 0.003363 | 0.001666 | 0.000437 | 0.001362 | 0.003598 | 0.001667 | 0.000503 | 0.000978 | 0.003457 | 0.001667 |
Nucleus | 0.029631 | 0.004061 | 0.078828 | 0.023481 | 0.001995 | 0.007816 | 0.106458 | 0.024721 | 0.004210 | 0.006353 | 0.089869 | 0.024169 |
Peroxisomes | 0.005903 | 0.002444 | 0.006244 | 0.013528 | 0.000325 | 0.001048 | 0.004504 | 0.010215 | 0.000772 | 0.001591 | 0.005549 | 0.011691 |
Vacuole | 0.642858* | 0.820501* | 0.499744* | 0.458783* | 0.886841* | 0.862527* | 0.446610* | 0.460797* | 0.867290* | 0.846156* | 0.478513* | 0.459900* |
Vacuole Periphery | 0.007387 | 0.034733 | 0.004985 | 0.005606 | 0.043147 | 0.021710 | 0.001198 | 0.009272 | 0.040281 | 0.026783 | 0.003471 | 0.007639 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.97 | -1.03 | -1.10 | -2.19 | -0.53 | -3.18 | -6.66 | -1.86 | -1.82 | -1.25 | 0.76 | -4.87 | -2.67 | -2.71 | -1.15 |
Bud Neck | -0.36 | -1.82 | -4.70 | -4.82 | -3.38 | -4.67 | -3.68 | -11.67 | -2.91 | -0.31 | -5.36 | -6.10 | -14.85 | -5.13 | -2.23 |
Bud Site | 1.60 | -1.02 | -3.29 | -8.64 | -2.01 | -0.79 | -4.33 | -12.06 | -11.70 | -5.46 | -1.69 | -4.67 | -15.12 | -14.21 | -3.15 |
Cell Periphery | -0.27 | 0.99 | 1.13 | 6.58 | 2.14 | -0.62 | 7.59 | 8.10 | 2.95 | 3.89 | -0.03 | 7.57 | 8.05 | 3.54 | 4.42 |
Cytoplasm | 8.07 | -2.86 | -5.77 | -25.03 | -2.40 | -1.33 | -13.90 | -28.84 | -27.97 | 2.49 | 1.35 | -18.44 | -37.27 | -37.82 | 0.10 |
Cytoplasmic Foci | 5.95 | -0.20 | -1.15 | -12.58 | -1.16 | -2.12 | -8.41 | -20.74 | -16.48 | -1.50 | -1.77 | -12.79 | -25.15 | -20.88 | -1.63 |
Eisosomes | 5.68 | -0.38 | 3.51 | -3.26 | 5.43 | -5.93 | -14.07 | -14.76 | -0.59 | 10.39 | -4.60 | -16.59 | -8.16 | -2.83 | 10.78 |
Endoplasmic Reticulum | 1.90 | -2.91 | 2.35 | 1.11 | 8.26 | -1.98 | -6.03 | 6.43 | 4.44 | 7.53 | -1.12 | -9.11 | 5.46 | 4.37 | 11.47 |
Endosome | -1.89 | -2.23 | -3.37 | -1.40 | -0.82 | -3.49 | -0.77 | -15.25 | -10.18 | -9.15 | -4.95 | -3.90 | -15.75 | -8.68 | -7.12 |
Golgi | 0.14 | 1.29 | -0.03 | -0.26 | -3.10 | -1.03 | 5.02 | -4.30 | -3.76 | -4.73 | -1.91 | 0.55 | -5.18 | -3.30 | -5.34 |
Lipid Particles | 0.22 | -4.41 | -2.30 | -1.16 | 3.64 | -2.13 | -8.68 | -5.56 | 0.94 | 5.69 | -2.29 | -7.62 | -7.22 | 0.28 | 5.23 |
Mitochondria | 1.25 | 1.50 | 0.24 | -1.99 | -2.55 | -0.75 | 2.02 | -1.73 | -1.02 | -3.74 | -0.09 | 2.06 | -1.89 | -2.04 | -4.20 |
Mitotic Spindle | 0.25 | -2.84 | -7.84 | -7.44 | -1.62 | -1.30 | -2.51 | -9.37 | -9.04 | -6.41 | -1.54 | -3.79 | -12.63 | -11.80 | -4.28 |
None | 0.07 | -3.36 | -4.02 | -3.56 | -1.18 | -0.71 | -4.87 | -3.38 | -3.25 | 1.11 | -1.18 | -6.70 | -5.28 | -4.71 | 0.09 |
Nuclear Periphery | 2.76 | -3.48 | -0.36 | -8.72 | 4.68 | -2.86 | -9.34 | -3.76 | -2.98 | 3.56 | -0.75 | -12.07 | -6.05 | -5.75 | 5.57 |
Nuclear Periphery Foci | 2.06 | -3.30 | 0.84 | -3.96 | 5.07 | -2.48 | -8.36 | -8.54 | -7.16 | 7.16 | 0.82 | -8.54 | -5.06 | -6.15 | 7.35 |
Nucleolus | 2.36 | -3.06 | -2.74 | -4.20 | 1.38 | -1.78 | -3.05 | -4.80 | -1.56 | 1.26 | -1.51 | -4.71 | -5.99 | -3.42 | 1.95 |
Nucleus | 5.39 | -5.37 | -0.66 | -14.88 | 5.87 | -4.46 | -9.63 | -14.96 | -11.03 | 7.25 | -1.83 | -13.57 | -19.42 | -17.25 | 9.21 |
Peroxisomes | 0.79 | -0.67 | -2.59 | -4.69 | -2.71 | -3.02 | -3.83 | -8.51 | -7.51 | -4.24 | -2.29 | -5.72 | -10.78 | -8.59 | -4.32 |
Vacuole | -6.58 | 6.70 | 8.45 | 23.94 | 0.80 | 2.80 | 21.60 | 45.72* | 34.59* | -1.20 | 3.03 | 29.87 | 53.37* | 41.95* | -0.09 |
Vacuole Periphery | -8.57 | 0.39 | 0.69 | 10.14 | 0.79 | 8.12 | 23.95 | 19.25 | 7.26 | -8.18 | 5.66 | 22.22 | 20.50 | 12.31 | -4.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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