Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.05 0 0 0.09 0 0.08 0.06 0.06 0 0 0.05 0.14 0.12 0.12 0.17 0.17 0.12 0.06 0.05 0.06 0.06 0.08 0.05
Bud 0.06 0.05 0 0 0 0 0 0 0.05 0.05 0.07 0.05 0.09 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.06 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.46 0.39 0.36 0.34 0.32 0.2 0.15 0.08 0.14 0.11 0.09 0.1 0.59 0.51 0.49 0.63 0.61 0.69 0.52 0.35 0.42 0.52 0.63 0.59
Cytoplasm 0.61 0.75 0.79 0.8 0.71 0.81 0.8 0.81 0.77 0.78 0.74 0.74 0.58 0.65 0.66 0.32 0.29 0.27 0.19 0.28 0.25 0.16 0.07 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.09 0.07 0 0.05 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.1 0.07 0.19 0.17 0.22 0.19 0 0 0 0.07 0 0 0 0 0.07 0.05 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 32 12 16 15 41 22 48 32 34 22 19 23 29 41 50 71 44 34 15 14 24 11 21 20
Bud 15 11 2 6 13 15 26 22 27 23 35 27 18 10 15 8 11 10 3 14 16 8 11 15
Bud Neck 0 2 13 9 18 10 20 16 11 13 4 8 0 1 0 0 1 0 9 24 24 12 12 18
Bud Site 1 0 0 0 2 1 1 0 2 1 0 1 0 1 0 0 0 0 – – – – – –
Cell Periphery 107 86 135 124 141 99 92 40 74 54 45 51 124 180 214 268 161 190 129 93 176 103 181 229
Cytoplasm 141 165 298 290 316 405 482 426 406 382 360 369 122 229 286 135 78 75 46 73 105 32 19 51
Endoplasmic Reticulum 4 6 6 1 3 7 5 8 4 4 0 4 1 1 4 20 3 19 23 18 10 10 12 22
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 2 2 3 0 0 1
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 3 2 1 0 2 2 1 0
Mitochondria 3 1 1 11 9 44 63 37 99 83 108 95 0 1 0 28 9 10 9 9 30 9 16 14
Nucleus 2 1 0 0 0 0 0 4 1 1 7 1 0 1 1 3 3 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 2 0 1 2 0 0 0 1 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 3 2 7 6 5 9 4 12 2 12 0 1 3 8 3 10 0 0 2 1 1 2
Vac/Vac Membrane 0 1 1 0 0 2 4 11 2 8 13 10 1 0 0 8 6 1 4 7 15 4 6 8
Unique Cell Count 232 219 376 363 442 497 604 529 524 491 488 499 209 352 434 428 266 277 251 264 424 201 288 387
Labelled Cell Count 308 285 476 458 552 611 749 607 664 605 596 601 295 467 577 551 322 356 251 264 424 201 288 387


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 6.4 6.1 5.7 6.1 4.8 4.9 5.2 4.3 4.5 4.3 4.8 7.0 7.0 7.2 10.8 10.6 10.7 6.2 7.0 6.8
Std Deviation (1e-4) 2.4 2.2 1.5 1.7 1.5 1.3 1.3 1.3 1.2 1.4 1.4 1.3 2.1 2.3 2.3 2.2 2.2 2.1 2.1 2.3 2.1
Intensity Change (Log2) – – – -0.1 0.01 -0.35 -0.31 -0.24 -0.52 -0.46 -0.5 -0.35 0.19 0.2 0.24 0.82 0.8 0.81 0.01 0.19 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 2.8 0 2.3 1.2 0 0 0 0 4.2 3.7 3.8 5.6 5.3 3.8
Bud 0 2.6 0 3.4 3.3 0 0 0 0 5.2 2.4 2.9 0 3.2 2.9
Bud Neck -0.8 0.5 0 -0.1 -0.4 0 0 0 0 0 -3.1 -3.9 -3.9 -2.6 -3.1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -1.2 0 -7.5 -10.6 0 0 0 0 5.5 4.1 3.8 7.6 6.2 8.3
Cytoplasm 0.2 -2.6 0.8 0.2 0.5 -0.6 -0.5 -1.9 -1.8 -5.4 -4.3 -4.2 -13.5 -12.7 -13.3
Endoplasmic Reticulum 0 0 0 0 -0.1 0 0 0 0 0 0 0 2.5 0 3.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 0 0 6.3 5.0 0 0 0 0 0 0 0 4.8 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1.3 0 2.5
Vacuole 0 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9378 1.8938 1.0914 1.795 0.8741 1.2894 1.1638 2.019 1.2883 1.3462 -0.1744 0.9986 0.9492 1.2072 0.3882 1.3135 0.9868 1.195
Actin 0.0672 0.0142 0.0084 0.0589 0.0934 0.0182 0.0332 0.0059 0.0217 0.0292 0.0177 0.0257 0.0557 0.0292 0.0212 0.0039 0.0124 0.0043
Bud 0.0012 0.0022 0.0015 0.0026 0.0094 0.003 0.0021 0.0014 0.0029 0.0009 0.0046 0.0011 0.0015 0.0005 0.0014 0.0018 0.0014 0.0005
Bud Neck 0.0086 0.0067 0.0272 0.0318 0.0138 0.0366 0.0055 0.0089 0.0395 0.0107 0.0678 0.0499 0.005 0.0057 0.0223 0.0205 0.04 0.0681
Bud Periphery 0.0083 0.0076 0.0141 0.0443 0.0421 0.0313 0.014 0.0038 0.0125 0.0069 0.0309 0.0128 0.0037 0.0016 0.0116 0.0166 0.0065 0.0063
Bud Site 0.0318 0.1714 0.0731 0.0132 0.0129 0.0043 0.0275 0.1809 0.0711 0.0059 0.0211 0.0037 0.0255 0.105 0.0401 0.0037 0.0303 0.0067
Cell Periphery 0.3241 0.2427 0.2602 0.4082 0.3326 0.3304 0.3763 0.3848 0.3435 0.3546 0.3098 0.3267 0.2392 0.1815 0.1897 0.3061 0.1096 0.2588
Cytoplasm 0.3222 0.4093 0.3505 0.3451 0.3168 0.4063 0.2895 0.3038 0.3202 0.2626 0.3492 0.3551 0.3373 0.4677 0.3955 0.5025 0.4901 0.4269
Cytoplasmic Foci 0.0151 0.0034 0.0141 0.0006 0.007 0.003 0.0139 0.0034 0.0095 0.0072 0.0069 0.0118 0.0189 0.0024 0.0053 0.0293 0.0226 0.0015
Eisosomes 0.0085 0.0035 0.0047 0.0034 0.0023 0.0024 0.0038 0.0019 0.0033 0.0015 0.0005 0.0009 0.0013 0.001 0.0007 0.001 0.0028 0.0017
Endoplasmic Reticulum 0.0097 0.0031 0.0042 0.0037 0.009 0.0067 0.0053 0.0027 0.0032 0.0056 0.0036 0.003 0.0117 0.0118 0.0081 0.0121 0.002 0.0039
Endosome 0.0101 0.0005 0.0066 0.0017 0.0184 0.0042 0.0185 0.0023 0.0055 0.0308 0.0318 0.0066 0.004 0.0003 0.0023 0.0011 0.0004 0.0002
Golgi 0.006 0.0012 0.0036 0.0053 0.015 0.0011 0.0138 0.001 0.0045 0.0297 0.0131 0.0063 0.0042 0.0007 0.0085 0.0007 0.0005 0.0003
Lipid Particles 0.0062 0.0007 0.0054 0.0011 0.0067 0.0013 0.0163 0.0006 0.0025 0.0077 0.002 0.0061 0.0124 0.0015 0.0037 0.0041 0.0023 0.0004
Mitochondria 0.003 0.0002 0.0045 0.0007 0.0154 0.0013 0.0114 0.0005 0.006 0.0581 0.0063 0.002 0.0008 0.0001 0.006 0.0005 0.0004 0.0001
None 0.1596 0.1293 0.2046 0.0742 0.0772 0.1378 0.1552 0.0946 0.1389 0.1775 0.1266 0.1815 0.2644 0.1882 0.2709 0.0809 0.2724 0.218
Nuclear Periphery 0.0025 0.0003 0.0005 0.0013 0.0071 0.0047 0.0013 0.0003 0.0004 0.0004 0.0006 0.0002 0.0009 0.0004 0.0009 0.0007 0.0002 0.0002
Nucleolus 0.0007 0.0003 0.0026 0.0002 0.0008 0.0003 0.0005 0.0002 0.0005 0.0002 0.0002 0.0002 0.0007 0.0001 0.0001 0.0006 0.0011 0.0001
Nucleus 0.0017 0.0006 0.0015 0.0005 0.0029 0.0011 0.0008 0.0005 0.0009 0.0004 0.0007 0.0004 0.0005 0.0004 0.0005 0.0009 0.0007 0.0003
Peroxisomes 0.0044 0.0002 0.0075 0.0001 0.0041 0.0007 0.0055 0.0001 0.0052 0.0049 0.0004 0.0033 0.0081 0.0004 0.0065 0.0009 0.0002 0
Punctate Nuclear 0.0049 0.0003 0.0016 0.0002 0.0032 0.0004 0.001 0.0002 0.0017 0.0002 0.0003 0.0003 0.0016 0.0002 0.0002 0.0041 0.0008 0.0001
Vacuole 0.0038 0.0022 0.0033 0.0025 0.0087 0.0045 0.0039 0.002 0.006 0.0037 0.0052 0.0021 0.0024 0.0012 0.0042 0.0078 0.0031 0.0014
Vacuole Periphery 0.0005 0 0.0002 0.0003 0.0014 0.0005 0.0008 0.0001 0.0005 0.0015 0.0009 0.0002 0.0001 0 0.0002 0.0002 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8454 15.1386 35.1047 66.1825 47.0009 14.8097 19.6116 20.1777 48.8711 53.5981
Translational Efficiency 0.3471 0.5648 0.4836 0.6118 0.6707 0.5439 0.5444 0.5001 0.4686 0.5578

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
Localization
Cell Percentages cell periphery (22%), cytoplasm (19%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Faa3

Faa3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Faa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available