Standard name
Human Ortholog
Description Nuclear thiol peroxidase; functions as an alkyl-hydroperoxide reductase during post-diauxic growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.07 0.08 0.12 0.08 0.09 0.06 – 0 0 0 0.05 0.1 0.15 0.2 0.13 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0.16 0 – 0.13 0.06 0.18 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.81 0.88 0.83 0.85 0.81 0.88 – 0.85 0.85 0.82 0.81 0.89 0.82 0.76 0.84 0.83 0.83 0.83 0.8 0.82 0.68 0.65 0.65
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 – 0.13 0.08 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.15 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.06 0 0.07 0.1 0.09 0.19 0.18 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1
Bud 0 1 0 0 0 1 2 – 5 3 3 2 0 1 0 0 0 0 1 3 2 2 9 6
Bud Neck 0 0 0 0 0 0 0 – 1 0 2 2 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 12 1 – 17 9 20 21 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 12 19 37 26 29 29 – 15 18 9 23 17 43 60 19 14 13 4 3 5 3 4 9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 2 3 0 0 0 0 0 1 2
Endosome 0 3 0 5 1 0 1 – 0 3 0 0 0 1 2 0 1 0 5 0 0 1 1 4
Golgi 0 1 1 0 1 0 0 – 0 0 0 0 0 0 0 0 0 1 0 2 1 0 1 1
Mitochondria 9 17 2 12 2 51 16 – 55 31 66 69 3 0 1 3 3 6 2 0 1 2 5 3
Nucleus 181 134 203 262 294 263 443 – 353 407 306 371 158 240 229 121 105 116 196 135 206 106 210 206
Nuclear Periphery 1 0 1 5 15 4 8 – 8 14 3 3 0 0 1 0 0 0 0 0 0 0 0 1
Nucleolus 1 2 1 0 8 16 15 – 55 38 41 51 0 1 6 1 2 3 0 0 0 2 13 14
Peroxisomes 0 0 0 0 0 0 1 – 1 1 0 0 0 1 2 0 0 1 0 0 1 0 0 0
SpindlePole 1 0 4 8 5 1 3 – 1 5 1 8 0 0 4 0 0 1 2 2 4 2 9 11
Vac/Vac Membrane 23 24 7 10 13 10 11 – 2 12 6 2 3 9 14 5 8 6 17 16 21 29 56 48
Unique Cell Count 216 165 230 316 345 326 504 415 480 371 458 177 293 300 144 126 140 235 170 252 155 322 318
Labelled Cell Count 227 194 238 339 365 387 531 513 541 458 552 181 296 319 151 136 147 235 170 252 155 322 318


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.5 6.3 6.6 7.8 6.0 7.2 – 6.3 7.2 6.1 6.1 6.3 6.8 7.0 8.5 8.9 9.0 5.7 6.2 6.9
Std Deviation (1e-4) 0.9 1.5 1.9 2.0 2.2 2.2 1.9 – 1.7 2.2 1.9 1.7 1.7 2.0 2.0 2.9 2.5 2.8 2.0 1.9 2.8
Intensity Change (Log2) – – – 0.05 0.3 -0.08 0.18 – -0.0 0.18 -0.06 -0.05 -0.01 0.1 0.14 0.43 0.49 0.51 -0.16 -0.04 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 3.1 0 3.6 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 -0.3 0.3 -1.3 – -2.5 -2.5 -3.3 -1.7 0.5 2.3 3.8 1.5 0.9 0.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.1 0 5.8 1.9 – 5.3 3.3 6.4 5.8 0 0 0 0 0 0
Nucleus -1.7 -1.0 -2.4 -0.1 – -1.1 -1.2 -1.9 -2.4 0.3 -2.0 -3.5 -1.2 -1.3 -1.5
Nuclear Periphery 0 2.8 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.0 2.2 – 5.5 4.1 5.0 5.0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.6 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 0.5 0 -0.7 – 0 -0.4 0 0 0 0 0.9 0 1.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4787 -0.2525 -0.3616 -0.8535 -0.9824 -0.8784 2.5812 1.7689 1.5923 1.5384 1.4861 1.7457 4.363 3.7356 4.1867 3.9781 3.873 4.1002
Actin 0.0535 0 0.0007 0 0.0045 0.0003 0.0761 0.002 0.0029 0.0021 0.0002 0.0038 0.0385 0 0 0 0 0.0001
Bud 0.0005 0.0001 0.0001 0 0.0002 0 0.0005 0.0003 0.0001 0 0 0 0.0002 0 0 0 0 0
Bud Neck 0.0009 0.0002 0.0006 0.0002 0.0008 0.0013 0.0027 0.0014 0.0003 0.0001 0.0004 0.0013 0.0028 0.0001 0.0001 0.0001 0.0002 0.0009
Bud Periphery 0.0007 0.0001 0.0001 0 0.0002 0.0001 0.0011 0.0005 0.0001 0 0.0001 0.0001 0.0002 0 0 0 0 0
Bud Site 0.002 0.0003 0.0004 0 0.0005 0.0001 0.0013 0.0013 0.0002 0.0001 0.0002 0.0001 0.0007 0.0001 0 0 0.0001 0.0001
Cell Periphery 0.0002 0 0 0 0.0001 0 0.0002 0.0001 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0162 0.0141 0.0209 0.0223 0.0245 0.0068 0.0097 0.0087 0.0055 0.0006 0.0009 0.0005 0.0058 0.0146 0.0031 0.0023 0.0011 0.006
Cytoplasmic Foci 0.0191 0.0032 0.0125 0.0002 0.012 0.001 0.0124 0.0002 0.0023 0.0026 0.0031 0.0011 0.0046 0.0002 0 0 0 0.0004
Eisosomes 0.0003 0 0 0 0 0 0.0005 0 0.0001 0 0 0 0.0003 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0017 0.0006 0.0015 0.0002 0.0017 0.0002 0.0049 0.0009 0.0004 0.0002 0.0001 0.0006 0.005 0.0004 0.0001 0 0 0.0005
Endosome 0.0283 0.0089 0.0091 0.0001 0.0646 0.0002 0.0253 0.0014 0.0044 0.0051 0.0031 0.0028 0.01 0.0005 0 0 0 0.0004
Golgi 0.0139 0.0002 0.0043 0 0.071 0 0.0106 0.0003 0.0016 0.0051 0.0014 0.0016 0.0027 0 0 0 0 0
Lipid Particles 0.0352 0.0001 0.0039 0 0.0049 0.0002 0.0174 0.0005 0.0023 0.0159 0.0139 0.0083 0.0034 0 0 0 0 0.0001
Mitochondria 0.0212 0.0005 0.0007 0.0001 0.0075 0.0001 0.0069 0.0005 0.0037 0.0006 0.0008 0.0029 0.0007 0.0002 0.0001 0.0001 0.0002 0.0001
None 0.0133 0.0044 0.0488 0.0613 0.0061 0.0479 0.0094 0.0115 0.0173 0.0002 0.0004 0.0089 0.0087 0.0096 0.0197 0.0027 0.0003 0.0283
Nuclear Periphery 0.0116 0.0078 0.0151 0.0048 0.0434 0.0064 0.0318 0.0123 0.004 0.0008 0.0026 0.0083 0.0117 0.0066 0.0018 0.001 0.0046 0.0024
Nucleolus 0.0059 0.0065 0.0039 0.0027 0.0044 0.0088 0.0055 0.015 0.0036 0.0042 0.0268 0.0132 0.0035 0.0049 0.0031 0.0031 0.0068 0.0139
Nucleus 0.7462 0.9468 0.8695 0.904 0.7422 0.8896 0.7506 0.9254 0.9377 0.9386 0.9243 0.9315 0.8818 0.9526 0.9706 0.988 0.9806 0.9129
Peroxisomes 0.0119 0 0.0019 0 0.0012 0 0.0115 0.0001 0.0013 0.002 0.0145 0.0095 0.0092 0 0 0 0 0
Punctate Nuclear 0.013 0.003 0.0048 0.0038 0.0064 0.0365 0.0172 0.0162 0.0112 0.0215 0.0068 0.005 0.0091 0.0098 0.0012 0.0025 0.0059 0.0334
Vacuole 0.0029 0.0026 0.0009 0.0001 0.0022 0.0003 0.0018 0.0011 0.0008 0.0001 0.0003 0.0002 0.0005 0.0003 0.0001 0.0001 0.0001 0.0003
Vacuole Periphery 0.0017 0.0006 0.0004 0 0.0015 0.0001 0.0027 0.0003 0.0003 0.0001 0.0001 0.0003 0.0004 0.0001 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.0154 42.6212 33.8858 41.0752 34.2199 45.9497 46.9419 47.2643 55.8499 50.696
Translational Efficiency 2.6257 1.5762 1.8247 1.8968 1.9892 2.7954 1.8235 1.7701 1.7413 1.6269

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
197 1216 1766 1473 835 2605 1393 1963 1032 3821 3159 3436

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 718.13 793.17 938.33 843.27 697.10 811.60 937.76 947.37 701.11 805.73 938.08 902.74
Standard Deviation 117.09 111.48 136.51 169.67 84.42 109.49 140.51 149.53 91.93 110.46 138.29 166.64
Intensity Change Log 2 — 0.143385 0.385850 0.231750 — 0.219403 0.427853 0.442562 — 0.181330 0.406693 0.339439

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000248 0.000390 0.000417 0.000770 0.000106 0.000081 0.000492 0.000528 0.000133 0.000180 0.000450 0.000632
Bud Neck 0.005050 0.014927 0.016296 0.017712 0.005351 0.008822 0.014499 0.019477 0.005293 0.010765 0.015503 0.018720
Bud Site 0.000774 0.004274 0.001366 0.009573 0.001135 0.001878 0.002056 0.006748 0.001066 0.002641 0.001670 0.007959
Cell Periphery 0.000307 0.001031 0.000104 0.000287 0.000173 0.000291 0.000114 0.000189 0.000199 0.000526 0.000108 0.000231
Cytoplasm 0.010201 0.085954 0.003213 0.055973 0.118387* 0.094941 0.009777 0.042336 0.097735 0.092081 0.006107 0.048182
Cytoplasmic Foci 0.002158 0.009257 0.000017 0.001315 0.022818 0.004159 0.000326 0.000963 0.018874 0.005781 0.000153 0.001114
Eisosomes 0.000027 0.000024 0.000021 0.000025 0.000031 0.000015 0.000020 0.000021 0.000030 0.000018 0.000021 0.000023
Endoplasmic Reticulum 0.004353 0.003982 0.003925 0.004597 0.001306 0.001661 0.005110 0.003950 0.001888 0.002400 0.004447 0.004227
Endosome 0.000368 0.001418 0.000182 0.001968 0.001003 0.000852 0.000542 0.001318 0.000882 0.001032 0.000341 0.001597
Golgi 0.000062 0.000412 0.000038 0.001496 0.000202 0.000089 0.000604 0.000375 0.000176 0.000191 0.000287 0.000856
Lipid Particles 0.000737 0.001017 0.000045 0.000316 0.002817 0.000642 0.000097 0.000383 0.002420 0.000761 0.000068 0.000354
Mitochondria 0.000532 0.002307 0.001113 0.003272 0.001951 0.001098 0.002002 0.003049 0.001680 0.001483 0.001505 0.003144
Mitotic Spindle 0.000174 0.004696 0.000785 0.023693 0.001197 0.001766 0.005203 0.016697 0.001001 0.002699 0.002733 0.019696
None 0.008107 0.006593 0.002094 0.016781 0.009902 0.016201 0.002993 0.007530 0.009559 0.013143 0.002490 0.011496
Nuclear Periphery 0.000440 0.001679 0.000628 0.001487 0.001353 0.001345 0.000969 0.001642 0.001179 0.001451 0.000778 0.001575
Nuclear Periphery Foci 0.000235 0.000472 0.000039 0.001303 0.000540 0.000219 0.000177 0.000568 0.000482 0.000299 0.000100 0.000883
Nucleolus 0.008000 0.004186 0.001528 0.002679 0.003936 0.004496 0.001377 0.001242 0.004711 0.004397 0.001461 0.001858
Nucleus 0.957017* 0.849863* 0.966958* 0.844981* 0.822600* 0.857169* 0.949991* 0.883489* 0.848259* 0.854844* 0.959476* 0.866981*
Peroxisomes 0.000325 0.001538 0.000056 0.000231 0.001769 0.000332 0.000098 0.000227 0.001493 0.000716 0.000074 0.000228
Vacuole 0.000737 0.005611 0.001072 0.009563 0.002895 0.003611 0.003021 0.008361 0.002483 0.004248 0.001931 0.008876
Vacuole Periphery 0.000147 0.000368 0.000103 0.001981 0.000527 0.000331 0.000535 0.000907 0.000455 0.000343 0.000293 0.001367

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.46 -6.14 -8.33 -3.64 -5.20 2.08 -15.94 -11.33 -12.51 -1.32 -1.21 -18.75 -15.10 -11.34 -4.80
Bud Neck -6.06 -8.83 -8.31 -1.68 0.11 -2.79 -7.78 -10.66 -9.94 -2.16 -5.34 -10.91 -12.57 -9.01 -1.57
Bud Site -4.37 -3.63 -6.52 -2.44 -5.67 -2.12 -3.06 -6.03 -5.09 -4.65 -4.44 -3.37 -8.60 -5.68 -7.46
Cell Periphery -0.87 1.54 0.66 0.99 -5.91 -2.86 3.47 1.34 3.73 -2.47 -1.24 3.23 0.76 1.34 -5.85
Cytoplasm -13.29 2.05 -7.99 7.35 -14.24 2.98 15.99 11.67 14.79 -12.08 0.77 16.17 9.71 15.96 -19.33
Cytoplasmic Foci -4.51 2.29 0.98 5.74 -2.47 7.28 8.95 8.79 7.45 -2.16 6.06 9.07 8.70 8.66 -3.40
Eisosomes 0.32 0.88 -1.36 -2.44 -5.23 6.90 4.00 2.51 -9.47 -3.02 6.02 4.09 1.66 -9.11 -5.64
Endoplasmic Reticulum 0.38 -0.06 -1.30 -3.67 -2.77 -1.73 -14.37 -13.89 -12.42 1.94 -2.50 -12.92 -13.24 -12.95 -0.67
Endosome -4.77 1.90 -2.68 0.46 -3.17 1.45 3.06 0.97 -0.13 -1.75 -0.39 4.36 -0.30 -0.02 -3.80
Golgi -2.03 0.88 -2.05 -0.98 -2.10 2.02 -0.64 -0.12 -1.72 0.59 -0.03 -0.14 -1.70 -1.67 -1.28
Lipid Particles -0.77 1.52 0.95 5.50 -7.29 5.06 6.43 5.55 1.49 -2.40 4.61 6.65 5.77 3.76 -3.90
Mitochondria -3.32 -6.78 -7.31 -0.81 -4.94 1.57 -0.33 -1.59 -4.82 -1.55 0.41 0.08 -2.51 -4.47 -4.28
Mitotic Spindle -3.69 -2.55 -7.58 -5.28 -7.09 -0.82 -3.66 -7.18 -6.90 -2.74 -2.64 -3.52 -10.12 -8.87 -7.42
None 0.52 2.22 -1.28 -3.93 -8.44 -3.03 4.17 1.90 6.84 -7.43 -2.16 4.95 0.41 3.95 -11.28
Nuclear Periphery -8.41 -4.28 -11.16 -0.75 -9.40 0.04 0.75 -3.55 -4.72 -6.09 -1.76 1.42 -4.95 -4.58 -10.59
Nuclear Periphery Foci -2.10 0.63 -1.46 -0.76 -1.70 1.67 1.80 1.03 -1.17 -1.44 1.30 1.89 0.24 -1.38 -2.18
Nucleolus 2.78 5.24 4.37 2.77 -2.34 -0.05 4.59 4.43 8.94 -0.85 1.08 6.13 5.29 7.81 -2.22
Nucleus 11.80 -0.76 10.86 -1.76 16.45 -3.66 -12.95 -6.68 -4.69 10.57 -0.85 -14.02 -3.57 -4.35 19.99
Peroxisomes -2.28 3.02 1.17 2.51 -2.51 2.30 2.68 2.53 1.47 -1.46 1.48 2.78 2.56 2.87 -2.70
Vacuole -6.18 -2.89 -11.13 -5.16 -10.58 -0.63 -1.56 -8.38 -7.02 -6.86 -2.86 0.02 -12.38 -8.93 -12.20
Vacuole Periphery -3.04 1.05 -2.87 -2.17 -3.02 0.72 0.46 0.08 -0.97 -0.52 0.50 1.01 -1.12 -2.34 -2.90
Standard name
Human Ortholog
Description Nuclear thiol peroxidase; functions as an alkyl-hydroperoxide reductase during post-diauxic growth
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Dot5

Dot5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dot5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available