Standard name
Human Ortholog
Description Aspartyl protease; secreted into the periplasmic space of mating type a cell; helps cells find mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.14 0.27 0.47 0.28 0.35 0.37 – 0.48 0.52 0.53 0.64 – 0.63 0.8 0.95 0.94 0.93 0 0 0.05 0 0 0.07
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 – 0 0 0 0 – 0 0 0.07 0 0.06 0 0 0 0.4 0.38 0.4
Endosome 0 0 0.06 0 0 0 0 – 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0 0 – 0 0.05 0.06 0 – 0 0 0 0 0 0 0 0 0.11 0 0
Nucleus 0.2 0.11 0.18 0.16 0.14 0.17 0.23 – 0.19 0.21 0.1 0.09 – 0.07 0.09 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.71 0.82 0.6 0.52 0.73 0.68 0.63 – 0.52 0.48 0.5 0.44 – 0.28 0.19 0 0 0 0.84 0.85 0.87 0.25 0.35 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 1 0 – 0 2 0 0 – 1 2 0 1 0 0 0 0 0 0 1
Bud 0 0 0 1 0 0 1 – 3 3 1 3 – 1 0 0 1 0 2 3 1 1 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 – 0 1 2 1 4 0 0 0 1 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 1 0 – 0 1 3 4 1 0 0 0 0 0 0
Cytoplasm 11 22 24 102 77 100 153 – 190 216 177 241 – 77 138 283 251 172 1 3 5 0 0 8
Endoplasmic Reticulum 5 8 0 5 6 3 2 – 5 3 3 4 – 3 3 22 8 11 5 5 0 9 21 47
Endosome 5 5 5 6 6 8 2 – 2 3 1 0 – 7 2 3 2 3 6 6 2 0 3 2
Golgi 0 4 1 1 7 2 0 – 0 0 0 0 – 1 0 0 2 0 1 0 0 0 1 4
Mitochondria 11 0 1 1 1 6 3 – 9 19 20 12 – 0 0 7 5 2 1 1 0 2 0 0
Nucleus 27 16 16 36 40 48 94 – 76 86 34 35 – 9 16 3 2 1 1 0 0 0 2 0
Nuclear Periphery 1 0 0 0 0 0 0 – 2 0 0 1 – 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 – 0 0 1 0 1 0 0 0 0 0 0
SpindlePole 1 0 0 2 0 0 0 – 1 0 0 1 – 1 1 0 1 0 1 0 0 0 0 0
Vac/Vac Membrane 96 124 53 114 205 193 263 – 209 201 165 167 – 34 33 9 11 8 125 139 98 5 20 46
Unique Cell Count 136 152 88 219 279 282 416 399 416 333 377 123 172 297 268 185 150 164 113 23 57 117
Labelled Cell Count 158 179 100 269 343 361 518 497 533 402 466 134 197 333 289 204 150 164 113 23 57 117


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 9.6 8.0 7.8 10.5 10.4 9.9 – 7.8 7.3 6.9 6.6 – 8.1 6.5 5.9 5.5 5.5 – 14.2 16.6
Std Deviation (1e-4) 2.0 2.5 3.1 2.6 3.5 4.2 4.2 – 3.4 3.1 2.8 2.8 – 2.5 1.7 1.3 1.6 1.5 – 5.0 6.0
Intensity Change (Log2) – – – -0.03 0.4 0.37 0.31 – -0.03 -0.14 -0.22 -0.27 – 0.02 -0.3 -0.44 -0.54 -0.55 – 0.82 1.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cytoplasm 3.1 0.1 1.4 1.7 – 3.5 4.2 4.3 6.3 – 5.1 8.3 13.9 12.9 11.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – 0 0 2.6 0 0
Endosome 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nucleus -0.4 -0.9 -0.3 0.9 – 0.2 0.5 -2.1 -2.4 – -2.4 -2.1 0 0 -5.6
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Vacuole -1.3 2.4 1.4 0.5 – -1.3 -2.0 -1.8 -2.7 – -4.7 -6.7 -12.8 -11.9 -10.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8235 2.7905 1.732 0.8887 1.512 2.1013 2.0402 3.4024 1.896 1.0949 1.1185 2.7594 1.1444 2.3322 0.9927 0.7406 0.7383 1.7347
Actin 0.0019 0 0.0039 0.0002 0.0038 0.0003 0.0045 0 0.0114 0.0017 0 0.0021 0.0333 0.0002 0.0158 0.0018 0.0002 0.0044
Bud 0.0008 0.0016 0.0025 0.0187 0.0187 0.0035 0.0004 0.0008 0.0007 0.0005 0.0004 0.0005 0.0063 0.015 0.0051 0.0005 0.0011 0.0005
Bud Neck 0.0014 0.0014 0.0031 0.0021 0.0015 0.0028 0.0009 0.001 0.0027 0.0022 0.001 0.0038 0.0039 0.0017 0.0013 0.0029 0.0018 0.0027
Bud Periphery 0.0017 0.0037 0.0044 0.0232 0.0113 0.007 0.0014 0.0013 0.0017 0.0012 0.0006 0.0017 0.0019 0.0072 0.0046 0.0009 0.0042 0.0013
Bud Site 0.0025 0.0076 0.0061 0.0084 0.0029 0.0012 0.0013 0.0024 0.0024 0.0022 0.0009 0.001 0.0041 0.0275 0.01 0.0064 0.0013 0.001
Cell Periphery 0.0031 0.0058 0.004 0.002 0.0024 0.0042 0.0029 0.0075 0.0034 0.0014 0.0015 0.0037 0.002 0.0039 0.0027 0.0021 0.0053 0.0044
Cytoplasm 0.0104 0.0135 0.012 0.0069 0.0159 0.0209 0.0271 0.0183 0.0142 0.0107 0.0034 0.0075 0.0126 0.0263 0.0157 0.005 0.0099 0.016
Cytoplasmic Foci 0.0306 0.0148 0.0294 0.0554 0.0249 0.0265 0.0295 0.009 0.0326 0.012 0.0222 0.0181 0.0421 0.0146 0.0342 0.0195 0.0132 0.0249
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0.0001 0.0006 0 0 0.0003 0 0.0003 0 0 0
Endoplasmic Reticulum 0.0082 0.0062 0.0033 0.0018 0.0024 0.0042 0.0072 0.008 0.0031 0.0014 0.0018 0.0041 0.0058 0.0071 0.0047 0.0008 0.0014 0.0028
Endosome 0.2762 0.1443 0.2113 0.23 0.1944 0.2285 0.2319 0.1308 0.2306 0.1931 0.3781 0.1395 0.2458 0.1682 0.2224 0.153 0.1542 0.1651
Golgi 0.0129 0.0042 0.0076 0.0044 0.0167 0.0066 0.0154 0.0036 0.016 0.0125 0.0119 0.0059 0.015 0.005 0.0092 0.0044 0.0018 0.0045
Lipid Particles 0.0409 0.013 0.03 0.0289 0.025 0.0245 0.0586 0.0184 0.0293 0.0198 0.0154 0.0159 0.0481 0.0183 0.0303 0.0062 0.005 0.0149
Mitochondria 0.036 0.0175 0.0635 0.0043 0.0226 0.0244 0.0226 0.0082 0.0227 0.0068 0.0039 0.0119 0.0335 0.0121 0.0206 0.002 0.0027 0.0062
None 0.0333 0.0278 0.055 0.0236 0.0623 0.0199 0.0578 0.0164 0.0329 0.0744 0.0009 0.001 0.0329 0.0179 0.0589 0.0746 0.0025 0.013
Nuclear Periphery 0.012 0.0076 0.0065 0.0047 0.0043 0.0046 0.014 0.0041 0.0038 0.0022 0.0044 0.0047 0.0067 0.007 0.0105 0.0015 0.0016 0.0039
Nucleolus 0.0094 0.0071 0.0135 0.011 0.0054 0.004 0.0021 0.0037 0.0059 0.0037 0.004 0.003 0.0035 0.0047 0.0059 0.0076 0.0043 0.0026
Nucleus 0.0137 0.0092 0.0154 0.0193 0.0089 0.0065 0.0047 0.0041 0.0036 0.0055 0.002 0.0049 0.0046 0.0058 0.0057 0.0037 0.0044 0.0037
Peroxisomes 0.0076 0.0009 0.0064 0.0036 0.0069 0.0022 0.0167 0.001 0.0102 0.0071 0.0017 0.0074 0.0126 0.0019 0.0076 0.0098 0.0006 0.0015
Punctate Nuclear 0.0042 0.001 0.0044 0.005 0.0196 0.0008 0.0057 0.0002 0.0011 0.0044 0.0002 0.001 0.0066 0.0005 0.0015 0.0006 0.0002 0.0007
Vacuole 0.3754 0.5997 0.3969 0.4372 0.4216 0.5162 0.4121 0.6669 0.4478 0.5423 0.4117 0.6494 0.4056 0.5515 0.4339 0.613 0.7006 0.6146
Vacuole Periphery 0.1175 0.113 0.1207 0.1093 0.1284 0.0911 0.0826 0.0943 0.1237 0.0942 0.1342 0.1133 0.0728 0.1036 0.0989 0.0835 0.0838 0.1116

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9506 12.3017 31.5346 148.0505 69.3409 23.7563 17.668 21.1783 71.7075 75.1904
Translational Efficiency 0.9383 0.7067 1.181 0.7941 0.9564 0.6987 0.7799 0.6298 1.0839 0.9178

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Aspartyl protease; secreted into the periplasmic space of mating type a cell; helps cells find mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
Localization
Cell Percentages vacuole (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bar1

Bar1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bar1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available